FastQCFastQC Report
Fri 27 May 2016
SRR939438_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939438_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2275022
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93320.4101938354881843No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68760.30223883549257985No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49940.21951436074024777No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT27120.11920763843162835No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25530.11221869502800413No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA163500.030.0895291
GTACATG126050.028.1855851
GGTATCA116050.026.5872671
TACATGG130950.026.5833072
ACATGGG134150.025.353753
TATCAAC199650.024.3907152
ATCAACG205400.024.0069453
TCAACGC209300.023.6921274
CAACGCA209100.023.5720085
AACGCAG212850.023.1373796
GAGTACT96450.022.89905512-13
CATGGGG43750.022.5596434
GTATCGA2253.94557E-822.3340741
CATGGGA90050.022.1855554
GTACTTT105500.021.14061214-15
ACGCAGA231100.020.8907267
AGTACTT101000.020.65392712-13
CGCAGAG233100.020.5867168
CATTCCG4350.020.057989
ACGCGGA3653.6379788E-1219.9201477
Produced by FastQC (version 0.11.5)