FastQCFastQC Report
Fri 27 May 2016
SRR939438_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939438_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2275022
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131250.5769174979406793No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113190.4975336502240418No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79320.3486559690411785No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT41240.18127297230532277No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38160.1677346416869815No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29760.13081192181877802No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25720.11305385178692778No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT24540.107867088757823No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA139600.026.892881
GTATCAA188350.025.7280181
GTACATG149300.025.4635471
TACATGG151650.024.7197952
ACATGGG153650.023.2821223
CGCATAG2150.022.3254266-67
GAGTACT114750.020.94433612-13
TATCAAC231400.020.8758722
ATCAACG231850.020.7498343
TCAACGC231900.020.7085424
GTATAGG6900.020.6356581
ATAGCGG1150.003764637320.6138868
CATGGGA101200.020.5382464
CAACGCA233650.020.5353645
AGTACTT117600.020.41660312-13
AACGCAG237100.020.3165476
GTACTTT119800.020.02188714-15
ATGGGAG39550.019.3004365
ACGCATA2400.018.99690464-65
AGAGTAC205350.018.59603510-11
Produced by FastQC (version 0.11.5)