FastQCFastQC Report
Fri 27 May 2016
SRR939437_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939437_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1456827
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115850.7952213955397587No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91400.6273908981642982No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63800.43793806677114033No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA52430.35989173731678503No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA30440.20894725317419294No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA24030.1649475195064342No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21060.14456074743260525No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20160.13838293771326313No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17140.11765295398835963No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG109550.028.6278761
TACATGG110100.027.716612
GTATCAA174500.026.1859021
ACATGGG113000.025.7209873
GAGTACT99200.024.59634412-13
GGTATCA128850.024.4560781
ACGCATA3000.024.270227
CATGGGA84050.023.9086654
TATCAAC204900.022.5062032
AACGCAT3250.022.392186
GTACTTT110100.022.18509714-15
GTATAGG5000.021.9732631
ATCAACG213000.021.8506363
TCAACGC215350.021.7955534
CAACGCA216500.021.719155
AGTACTT102800.021.42904312-13
CATGGGG28750.021.3017794
CGCATAG2558.407369E-921.1215088
AACGCAG219400.021.1181166
CATTCCG2358.1150574E-820.8608659
Produced by FastQC (version 0.11.5)