FastQCFastQC Report
Fri 27 May 2016
SRR939437_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939437_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1456827
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158301.0866080873020612No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141370.9703966222482149No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101710.6981611406158726No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA76740.5267612420692368No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA40850.28040391892791666No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA34750.23853209749682014No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33930.2329034264191973No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33930.2329034264191973No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25720.17654807331275435No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT22560.15485709696484207No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG18730.12856708449253068No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA17550.12046728952717105No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT16320.11202428291073682No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16190.11113193261794298No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15790.10838623940934648No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15580.10694475047483333No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTC1452.4672772E-829.4001887
ATGGGCG2500.026.5255035
GGTATCA140700.026.3042771
GTATCAA193050.025.9953441
ATACCGT1109.660776E-525.8365276
GAGTACT116850.023.450412-13
GTACATG119050.022.7685851
TACATGG119450.022.21172
GTACTTT124100.021.90865514-15
TATCAAC233250.021.4497492
CATTCCG2658.221832E-1021.4491949
ATCAACG235300.021.1973743
TCAACGC236950.021.0497654
AGTACTT119450.021.01673912-13
ACATGGG120350.020.9776443
CAACGCA239650.020.7533135
AACGCAG242450.020.4941026
ACTTTTT140250.019.94309616-17
GCACCGT2151.1086177E-619.8280336
CATGGGG28650.019.5089074
Produced by FastQC (version 0.11.5)