##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939437_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1456827 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.960977521696126 31.0 31.0 31.0 30.0 34.0 2 31.188311309441684 31.0 31.0 34.0 30.0 34.0 3 31.157400981722606 31.0 31.0 34.0 28.0 34.0 4 35.11199476670875 35.0 35.0 37.0 32.0 37.0 5 34.7562675595661 35.0 35.0 37.0 32.0 37.0 6 34.73567142838511 35.0 35.0 37.0 32.0 37.0 7 34.60249157930214 35.0 35.0 37.0 30.0 37.0 8 34.623132327997766 35.0 35.0 37.0 30.0 37.0 9 35.930200360097665 37.0 35.0 39.0 30.0 39.0 10-11 35.789234754710066 37.0 35.0 39.0 30.0 39.0 12-13 35.754367539865754 37.0 35.0 39.0 30.0 39.0 14-15 36.504753138155735 38.0 35.0 40.0 30.0 41.0 16-17 36.54522740174365 38.0 35.0 40.0 30.5 41.0 18-19 36.607245747092826 38.0 35.0 40.0 30.0 41.0 20-21 36.55819634040281 38.0 35.0 40.0 30.0 41.0 22-23 36.413524735606906 38.0 35.0 40.0 30.0 41.0 24-25 36.19798884836703 38.0 34.0 40.0 29.5 41.0 26-27 35.79126073308636 38.0 34.0 40.0 28.0 41.0 28-29 35.50040773544148 38.0 34.0 40.0 27.0 41.0 30-31 35.21496443984083 37.5 34.0 40.0 27.0 41.0 32-33 34.89966584913651 37.0 33.0 40.0 27.0 41.0 34-35 34.756637884937604 37.0 33.0 40.0 25.5 41.0 36-37 34.641212374564724 37.0 33.0 40.0 25.0 41.0 38-39 34.5471610561858 37.0 33.0 40.0 25.0 41.0 40-41 34.28111642631555 37.0 33.0 40.0 24.5 41.0 42-43 33.914923323085034 37.0 33.0 40.0 23.5 41.0 44-45 33.61745423444239 37.0 33.0 40.0 23.0 41.0 46-47 33.14061861840836 37.0 32.0 40.0 20.0 41.0 48-49 32.72867849099447 37.0 31.0 40.0 16.5 41.0 50-51 32.442658943031674 36.5 31.0 40.0 15.0 41.0 52-53 32.059304570824125 36.0 31.0 39.0 12.0 41.0 54-55 31.86163319323434 36.0 30.0 39.0 10.0 40.0 56-57 31.504555448244712 35.5 30.0 39.0 8.0 40.0 58-59 31.10516554127566 35.0 29.0 39.0 7.5 40.0 60-61 30.826394280171908 35.0 29.0 39.0 6.5 40.0 62-63 30.448597877441863 35.0 28.0 38.0 2.0 40.0 64-65 30.028855176352444 34.0 28.0 38.0 2.0 40.0 66-67 28.384952365654947 33.5 24.5 37.5 2.0 39.5 68-69 24.819941214708404 29.5 13.0 35.0 2.0 39.0 70-71 24.228904324260878 28.5 10.5 35.0 2.0 38.0 72-73 26.020095385382067 31.0 20.0 35.0 2.0 38.0 74-75 26.608439780426913 31.0 22.0 35.0 2.0 38.0 76-77 26.614797776263067 31.5 24.0 34.5 2.0 36.5 78-79 27.40585155272383 32.5 26.0 35.0 2.0 37.0 80-81 27.264712282240787 33.0 26.0 35.0 2.0 37.0 82-83 26.85377639211794 32.5 25.0 35.0 2.0 36.0 84-85 26.50259673935203 32.0 24.5 35.0 2.0 36.0 86-87 26.0402436253584 32.0 23.5 35.0 2.0 35.5 88-89 25.528824630515494 32.0 21.0 34.0 2.0 35.0 90-91 25.05788813634014 31.0 19.5 34.0 2.0 35.0 92-93 24.518137362912686 31.0 17.0 34.0 2.0 35.0 94-95 23.193444726106804 30.0 4.5 34.0 2.0 35.0 96-97 22.40476254215497 30.0 2.0 34.0 2.0 35.0 98-99 22.07406198539703 30.0 2.0 34.0 2.0 35.0 100-101 20.885332644164336 28.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3821529431925583 1101 2 0.31052804703681147 1101 3 0.38433582360084273 1101 4 0.35038916939822684 1101 5 0.4014489401788168 1101 6 0.47507921116223173 1101 7 0.5029545414978216 1101 8 0.48154352891717167 1101 9 0.7298678277929653 1101 10-11 0.8667916423928261 1101 12-13 0.6295577330783217 1101 14-15 0.7321609198125785 1101 16-17 0.6193391473796197 1101 18-19 0.7568926758999339 1101 20-21 0.7995432171115695 1101 22-23 0.8609443458471802 1101 24-25 0.618326877957216 1101 26-27 0.6791013578229936 1101 28-29 0.7261910868166837 1101 30-31 0.6446480527141176 1101 32-33 0.6953643787145154 1101 34-35 0.6954936364382434 1101 36-37 0.6222562301046182 1101 38-39 0.6655807511396432 1101 40-41 0.8580406380603947 1101 42-43 0.8424000697538361 1101 44-45 0.45990777965594276 1101 46-47 0.20829815219010328 1101 48-49 0.386937393076316 1101 50-51 0.2594991993139075 1101 52-53 0.6024362720701184 1101 54-55 0.41679162912582157 1101 56-57 0.3767735993180743 1101 58-59 0.3342400835894992 1101 60-61 0.26761127529247375 1101 62-63 0.03437988982944162 1101 64-65 -0.1409041194852314 1101 66-67 -2.7673953105619873 1101 68-69 -7.497325598066002 1101 70-71 -10.910191260750107 1101 72-73 -10.745681630995762 1101 74-75 -9.14380915739127 1101 76-77 -4.385602997820094 1101 78-79 -1.428997543193276 1101 80-81 -0.8233466788887966 1101 82-83 -0.5979057821496916 1101 84-85 -0.7041483552157892 1101 86-87 -0.6482824374281826 1101 88-89 -0.7021782942558978 1101 90-91 -0.7970376967658517 1101 92-93 -1.4427503234343604 1101 94-95 -4.993545077102624 1101 96-97 -1.8549088293256055 1101 98-99 -0.9898019485208636 1101 100-101 -0.7446213806845705 1102 1 0.39464558069699507 1102 2 0.21065364034529566 1102 3 0.37852525967489825 1102 4 0.2200741220309652 1102 5 0.3500163821368716 1102 6 0.4402113314260703 1102 7 0.4139758788717245 1102 8 0.45493962893021234 1102 9 0.6753876087390509 1102 10-11 0.6581782344828468 1102 12-13 0.5194282880418868 1102 14-15 0.5679360538362204 1102 16-17 0.5856994742819168 1102 18-19 0.6631739149873255 1102 20-21 0.8723730981276461 1102 22-23 0.7893430461512736 1102 24-25 0.6736328954953237 1102 26-27 0.7931713303813126 1102 28-29 0.7206502937204107 1102 30-31 0.6427027052867516 1102 32-33 0.26623767185198943 1102 34-35 0.4641089477312974 1102 36-37 0.5368396664754229 1102 38-39 0.43765212650626495 1102 40-41 0.5131348854971876 1102 42-43 0.7540313889481851 1102 44-45 0.43292395241719106 1102 46-47 0.36670645933318724 1102 48-49 0.4362983359553425 1102 50-51 0.37201012128569033 1102 52-53 0.28695202593720737 1102 54-55 0.33043723718177986 1102 56-57 0.3167642023008419 1102 58-59 0.18634571985149506 1102 60-61 0.0791943354828426 1102 62-63 0.19291483938979326 1102 64-65 0.12488084618643214 1102 66-67 -3.006945812410663 1102 68-69 -7.799416097186999 1102 70-71 -10.796071455524647 1102 72-73 -9.093895073676208 1102 74-75 -7.628888582419709 1102 76-77 -4.28764621210955 1102 78-79 -1.7260740516992996 1102 80-81 -0.8255737869604935 1102 82-83 -0.5740625369864567 1102 84-85 -0.48608022908893034 1102 86-87 -0.4814276805441544 1102 88-89 -0.7452014960459756 1102 90-91 -0.6889974769326024 1102 92-93 -1.1213985617559068 1102 94-95 -4.136251223231486 1102 96-97 -0.919196716765228 1102 98-99 -0.18677174767648097 1102 100-101 2.926898125430455E-4 1103 1 0.33996438210525426 1103 2 0.26764598719427823 1103 3 0.3468368408043645 1103 4 0.29913471440687545 1103 5 0.49195016766441313 1103 6 0.4953800729586604 1103 7 0.5922948967160977 1103 8 0.5774926464166015 1103 9 0.7097088343815656 1103 10-11 0.6750802847843929 1103 12-13 0.6014798552565352 1103 14-15 0.7533684430138194 1103 16-17 0.6351880784337425 1103 18-19 0.5851548746006472 1103 20-21 0.7529056058583095 1103 22-23 0.6994754391483298 1103 24-25 0.8509622236371825 1103 26-27 0.6587781715598879 1103 28-29 0.6748158276548253 1103 30-31 0.5560074975118283 1103 32-33 0.6863704992191018 1103 34-35 0.5405341121366263 1103 36-37 0.4692056277401022 1103 38-39 0.6931203212568278 1103 40-41 0.7149649314730127 1103 42-43 0.6092770064913395 1103 44-45 0.41133838189084315 1103 46-47 0.4112144116753029 1103 48-49 0.32094518935619476 1103 50-51 0.20005256523135984 1103 52-53 0.4833451443172798 1103 54-55 0.46408223102944746 1103 56-57 0.41951163034331884 1103 58-59 0.5740133174181246 1103 60-61 0.3306159612700412 1103 62-63 0.3143132188899216 1103 64-65 0.29325166640441225 1103 66-67 -2.7281804044435702 1103 68-69 -7.521672769448056 1103 70-71 -10.998728115203946 1103 72-73 -8.789408779923718 1103 74-75 -7.31448348591092 1103 76-77 -3.814638379219204 1103 78-79 -0.9835358552187472 1103 80-81 -0.2257952407970656 1103 82-83 0.03815266042349563 1103 84-85 -0.07983114182773221 1103 86-87 -0.03600459287704538 1103 88-89 0.2571791139743418 1103 90-91 0.24093208022712886 1103 92-93 0.10642291514721691 1103 94-95 0.9961080726995881 1103 96-97 1.3342876050969927 1103 98-99 0.8043833920820873 1103 100-101 0.4226434256578848 1104 1 0.49549840288311486 1104 2 0.2392570112835095 1104 3 0.422741708433513 1104 4 0.20265133793514423 1104 5 0.4565455839396009 1104 6 0.5553764576428435 1104 7 0.549365572495887 1104 8 0.533218147731894 1104 9 0.7718488972559925 1104 10-11 0.7794020085319886 1104 12-13 0.7496163275975434 1104 14-15 0.9511409784216838 1104 16-17 0.9307587334400438 1104 18-19 0.9231978370895604 1104 20-21 0.981437481500187 1104 22-23 0.727513816891971 1104 24-25 0.9114238251200462 1104 26-27 0.9889889823746358 1104 28-29 0.9590897140683126 1104 30-31 0.8571487273460576 1104 32-33 0.7408418649702568 1104 34-35 0.8442485211381765 1104 36-37 0.905492610926693 1104 38-39 1.0284791304184466 1104 40-41 1.0251998468228791 1104 42-43 1.0661685564924 1104 44-45 0.9613073674375983 1104 46-47 1.024380721305242 1104 48-49 0.9629884696430295 1104 50-51 0.8384486405727927 1104 52-53 0.8910135498828247 1104 54-55 0.9658993645650717 1104 56-57 1.3033313427070432 1104 58-59 1.2859171091992572 1104 60-61 1.0616111352607618 1104 62-63 0.9924739415449046 1104 64-65 0.7280181760429478 1104 66-67 -2.145235511040717 1104 68-69 -6.609576695345741 1104 70-71 -8.049122775032611 1104 72-73 -7.223286038493875 1104 74-75 -6.246614678141487 1104 76-77 -2.984303109699596 1104 78-79 -0.14418142962756164 1104 80-81 0.637853232424451 1104 82-83 0.6902543178562901 1104 84-85 0.7862915631573948 1104 86-87 1.0194919627328538 1104 88-89 0.9825264503393889 1104 90-91 0.5508284241661165 1104 92-93 0.7665839570579074 1104 94-95 1.5592884499898645 1104 96-97 1.8092411563065554 1104 98-99 1.5395788195822462 1104 100-101 1.2179493098030498 1105 1 0.5600250846049519 1105 2 0.38816902898790673 1105 3 0.5850699634106107 1105 4 0.22807125960404306 1105 5 0.30917878149499955 1105 6 0.47542475881057555 1105 7 0.6281157722432269 1105 8 0.5763650803665357 1105 9 0.8543543469663959 1105 10-11 0.8490949855663246 1105 12-13 0.8228048830549426 1105 14-15 1.0832410540995454 1105 16-17 1.1576833932665593 1105 18-19 1.0850523516809147 1105 20-21 0.9256129998633824 1105 22-23 1.1051180699273857 1105 24-25 1.181683858466542 1105 26-27 1.202334968885289 1105 28-29 1.0774305900621428 1105 30-31 0.8671948878253346 1105 32-33 0.9848253243831024 1105 34-35 1.3317507447406243 1105 36-37 1.2375224358425 1105 38-39 1.193461009942908 1105 40-41 1.2410362680741827 1105 42-43 1.2473454225848002 1105 44-45 1.14639664319143 1105 46-47 0.9495414236922599 1105 48-49 0.7088891266233333 1105 50-51 0.8697765608788757 1105 52-53 1.2177248192507726 1105 54-55 1.0092200396960003 1105 56-57 0.9172871992990075 1105 58-59 1.0005509007203237 1105 60-61 1.0349463731033097 1105 62-63 1.041179880297097 1105 64-65 0.8483418270323355 1105 66-67 1.7372278786640791 1105 68-69 -4.931765006583753 1105 70-71 -5.0098124988235 1105 72-73 -1.4648314497321913 1105 74-75 1.1503978554386762 1105 76-77 1.3621680709051667 1105 78-79 1.1906859381397048 1105 80-81 1.259230644844667 1105 82-83 1.4140614116757284 1105 84-85 1.1740855686267047 1105 86-87 1.2760184243557369 1105 88-89 1.3096485208373885 1105 90-91 1.4179353892427429 1105 92-93 1.4902666798930255 1105 94-95 2.098826362788472 1105 96-97 2.1326317203930394 1105 98-99 1.9742327968909485 1105 100-101 1.7291110403235344 1106 1 0.27194849887706596 1106 2 0.2678261324341129 1106 3 0.259634220905383 1106 4 0.1424453272087831 1106 5 0.36729727346983765 1106 6 0.42054331825521984 1106 7 0.40432366920933305 1106 8 0.30364687176489014 1106 9 0.5514119137052802 1106 10-11 0.5458920438827306 1106 12-13 0.4131613860198655 1106 14-15 0.3594223379750048 1106 16-17 0.5313501149086264 1106 18-19 0.5745096320479561 1106 20-21 0.6848567382904278 1106 22-23 0.6741499906428601 1106 24-25 0.6144994859732336 1106 26-27 0.7453649629629382 1106 28-29 0.9543289280558938 1106 30-31 0.9760086936472305 1106 32-33 0.946676021196275 1106 34-35 0.923283846177732 1106 36-37 0.8736090370884355 1106 38-39 0.8985154355874414 1106 40-41 0.7949465182372322 1106 42-43 0.7480058770314315 1106 44-45 0.827350104017718 1106 46-47 0.7887359466432358 1106 48-49 0.5534624925420815 1106 50-51 0.5275190960943661 1106 52-53 0.8016954923745594 1106 54-55 0.5302854254358884 1106 56-57 0.6443341582848596 1106 58-59 0.8721725375378462 1106 60-61 0.6879635854008086 1106 62-63 0.7950219109883605 1106 64-65 0.7659752485761935 1106 66-67 1.3180525846081679 1106 68-69 -5.4580784188317715 1106 70-71 -5.787230354333158 1106 72-73 -2.0959385917861226 1106 74-75 0.4892151097173887 1106 76-77 0.8193648503463677 1106 78-79 0.5937027756049353 1106 80-81 0.5513960883087172 1106 82-83 0.593088984336962 1106 84-85 0.6830995348768063 1106 86-87 0.7524191725209306 1106 88-89 0.5387662406577682 1106 90-91 0.35658659282913874 1106 92-93 0.6870444343479072 1106 94-95 1.6075003561764305 1106 96-97 1.637662156772759 1106 98-99 1.4600611387079852 1106 100-101 1.2504633601604098 1107 1 0.011920422416892507 1107 2 0.03438944900848284 1107 3 -0.11771302595774458 1107 4 -0.1614564694201519 1107 5 0.08938415178690917 1107 6 0.13087473762797686 1107 7 0.11187578354061145 1107 8 0.1347886918979384 1107 9 0.07354375970419369 1107 10-11 0.07976839031525884 1107 12-13 0.14299744292098637 1107 14-15 0.0863528780217564 1107 16-17 -0.04804750709455874 1107 18-19 0.2007471407628003 1107 20-21 0.16815370070462876 1107 22-23 0.24824936166213263 1107 24-25 0.33795844540596676 1107 26-27 0.09959938817960534 1107 28-29 0.27687984261918785 1107 30-31 0.060017014557772086 1107 32-33 0.16942154379443508 1107 34-35 0.08966143488971312 1107 36-37 0.20703967443920135 1107 38-39 -0.12264129189895812 1107 40-41 0.11537809461899684 1107 42-43 0.17215730097653648 1107 44-45 -0.06156347301021725 1107 46-47 0.094155497004202 1107 48-49 0.0252133413543163 1107 50-51 0.17571878283212072 1107 52-53 0.2454437564897738 1107 54-55 0.13890542774094783 1107 56-57 0.19330396380908255 1107 58-59 0.023087667651267907 1107 60-61 0.06315200565591539 1107 62-63 0.2551593836157018 1107 64-65 0.09710365398063203 1107 66-67 0.537561756692682 1107 68-69 -5.5652985658647545 1107 70-71 -5.6903083732611535 1107 72-73 -2.4208901759522057 1107 74-75 -0.12351830183704138 1107 76-77 0.007162926560919658 1107 78-79 0.11998903079841838 1107 80-81 -0.2310531010449033 1107 82-83 -0.1121217875011773 1107 84-85 0.057349302008450564 1107 86-87 0.21838411662308133 1107 88-89 0.05037357076778548 1107 90-91 0.15961869937609308 1107 92-93 0.18538610937516253 1107 94-95 0.7379304399334572 1107 96-97 0.8219763712837178 1107 98-99 0.7567480786194523 1107 100-101 0.5226463143859554 1108 1 0.039676469208128395 1108 2 -0.07484972030628612 1108 3 0.057418340740539975 1108 4 -0.06372703058730167 1108 5 0.03821287084333136 1108 6 0.13826404229025968 1108 7 0.17502410733153795 1108 8 0.12306434388522547 1108 9 0.15496111233925802 1108 10-11 0.20769774653189188 1108 12-13 0.2293319601123116 1108 14-15 0.3443271885340877 1108 16-17 0.28458716540601614 1108 18-19 0.29746868490131817 1108 20-21 0.39259113738668816 1108 22-23 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1.872238068562858 2311 68-69 4.2176579211148635 2311 70-71 4.454198825181173 2311 72-73 2.6694085287794884 2311 74-75 1.6242157845442655 2311 76-77 0.9000450758423639 2311 78-79 0.7580418086299261 2311 80-81 0.914488248896749 2311 82-83 0.8087688464646412 2311 84-85 0.6429556410218815 2311 86-87 0.6814273846449765 2311 88-89 0.8789236310503092 2311 90-91 0.9789929216670892 2311 92-93 1.0115126188559564 2311 94-95 1.4049910383744972 2311 96-97 1.6530731263432585 2311 98-99 1.3532433108199875 2311 100-101 1.1944018574766737 2312 1 -0.09439602536297187 2312 2 -0.03502893748307301 2312 3 0.0677153667877235 2312 4 -0.053263849032404664 2312 5 0.022154633337883922 2312 6 0.19654077890377408 2312 7 0.12000586484142417 2312 8 0.1304109558128843 2312 9 -1.1068815060869284 2312 10-11 -0.9707319223801747 2312 12-13 -1.796339642757566 2312 14-15 -1.9449166654429106 2312 16-17 0.11736837783352172 2312 18-19 -1.7959695571734073 2312 20-21 -1.560270314304752 2312 22-23 -0.7040645845542528 2312 24-25 -1.3665443688529493 2312 26-27 -1.5246970470924452 2312 28-29 0.008118116540387632 2312 30-31 0.597071423563392 2312 32-33 0.6331488733754185 2312 34-35 0.8995426106245432 2312 36-37 1.0366206625567713 2312 38-39 0.7311735924037279 2312 40-41 1.0792617719173094 2312 42-43 0.9096494835906697 2312 44-45 1.1180800541942801 2312 46-47 1.0977514945026385 2312 48-49 1.0195848235306784 2312 50-51 0.9996712531260528 2312 52-53 1.2351248071464376 2312 54-55 1.13906177545827 2312 56-57 1.0971501176552358 2312 58-59 0.8828437652122432 2312 60-61 1.1801000982137637 2312 62-63 0.5903688895569168 2312 64-65 1.1286858928799433 2312 66-67 2.536780018106068 2312 68-69 4.366221133947629 2312 70-71 5.052343284024651 2312 72-73 3.381799004785755 2312 74-75 2.4715411102955436 2312 76-77 1.4339921948536016 2312 78-79 1.4084505692599585 2312 80-81 1.3352383311001894 2312 82-83 1.4680845914856882 2312 84-85 1.2826463626713682 2312 86-87 1.3931808645905583 2312 88-89 1.1906174732068102 2312 90-91 1.5051471546606443 2312 92-93 1.3962991800443305 2312 94-95 1.8600380071629772 2312 96-97 2.184234032321868 2312 98-99 1.8027143075125096 2312 100-101 1.749975895374071 2313 1 -0.1786973446900788 2313 2 -0.1578589707863216 2313 3 0.011694939108881641 2313 4 0.14594395407537064 2313 5 -0.08009835439191448 2313 6 -0.050696726845657736 2313 7 -3.315620234663694 2313 8 -1.2191293265771677 2313 9 -0.5491421755343922 2313 10-11 -0.05555286407025051 2313 12-13 -1.4824987997285675 2313 14-15 -0.5989754100352798 2313 16-17 0.08752743364610893 2313 18-19 0.4223789209455475 2313 20-21 0.326338602494026 2313 22-23 -1.121118234871716 2313 24-25 -2.4980969451524757 2313 26-27 -2.038740285995445 2313 28-29 -0.3691881769359995 2313 30-31 -0.19118779805415897 2313 32-33 -2.195916312163355 2313 34-35 -2.5234655563374346 2313 36-37 -1.021101281391978 2313 38-39 -0.46218164278170093 2313 40-41 -0.4840400863945149 2313 42-43 -0.4464506853755097 2313 44-45 0.0646458838276942 2313 46-47 0.1571500602658915 2313 48-49 -0.03276545015027921 2313 50-51 0.26431907166048063 2313 52-53 0.37659960210305954 2313 54-55 0.3513202004532552 2313 56-57 0.7508101558109281 2313 58-59 0.07834387506396823 2313 60-61 0.5323115951845381 2313 62-63 0.28054356170088823 2313 64-65 0.32214900403882396 2313 66-67 1.5987984289388706 2313 68-69 4.581483094745575 2313 70-71 4.757086349535793 2313 72-73 2.8363085365961282 2313 74-75 2.2671062618514064 2313 76-77 1.2902595123061147 2313 78-79 0.9007921654450222 2313 80-81 1.0350017152287485 2313 82-83 0.8248288550918943 2313 84-85 1.0478106423680806 2313 86-87 1.2151776053926504 2313 88-89 1.1595108632942335 2313 90-91 1.1375864477529483 2313 92-93 1.522298712186629 2313 94-95 2.293217732486248 2313 96-97 2.232261759213092 2313 98-99 1.8931507270733867 2313 100-101 1.599711381906566 2314 1 -0.26375872960510804 2314 2 -0.2891776197289495 2314 3 -0.2767520520030864 2314 4 -0.14371735790078333 2314 5 -1.580830928290311 2314 6 -2.746711206328012 2314 7 -0.8861440090755224 2314 8 -0.19226058630169263 2314 9 0.052046983008928294 2314 10-11 0.12705869817543913 2314 12-13 -0.11341015350920003 2314 14-15 -2.807147431127042 2314 16-17 -3.040833050043773 2314 18-19 -0.6538406155262777 2314 20-21 -0.3659596570059449 2314 22-23 -0.239670191082908 2314 24-25 -2.438192705301418 2314 26-27 -1.3466255681080597 2314 28-29 -0.40455119119093297 2314 30-31 -0.2330516093813415 2314 32-33 -0.11926638852622062 2314 34-35 -0.19906551047193233 2314 36-37 0.14521966878822212 2314 38-39 -1.683517300179183 2314 40-41 -4.427045040823639 2314 42-43 -5.077149035332429 2314 44-45 -2.092707406305017 2314 46-47 -3.0775562651912125 2314 48-49 -0.9911588359635033 2314 50-51 -1.0667732756496164 2314 52-53 -2.4522468488152853 2314 54-55 -1.7110226345758797 2314 56-57 -3.4313861485130097 2314 58-59 -5.2525314870425355 2314 60-61 -2.8392104704190437 2314 62-63 -1.5185564075628584 2314 64-65 -1.0341164138547718 2314 66-67 -0.7288500247728109 2314 68-69 1.4915540747861264 2314 70-71 1.3452806822804142 2314 72-73 -0.46492585042266654 2314 74-75 -0.2032834196859774 2314 76-77 -0.4836140358837113 2314 78-79 -0.11279469154222 2314 80-81 0.15217850282850165 2314 82-83 -0.32912473475909465 2314 84-85 -0.3813422951736207 2314 86-87 -0.36533107162666667 2314 88-89 -0.061129247037765566 2314 90-91 0.21219113654440847 2314 92-93 0.26249774932971803 2314 94-95 0.13963560537830944 2314 96-97 0.33800482006609656 2314 98-99 0.05846518441926918 2314 100-101 0.15102818348892555 2315 1 -0.2549244378125337 2315 2 -0.2539500097285696 2315 3 -0.20549905661046353 2315 4 0.02407044380472456 2315 5 -1.1070828181514898 2315 6 -0.20498586017715326 2315 7 0.07400108445641962 2315 8 -0.05283713588826089 2315 9 0.04790273406316459 2315 10-11 -1.7692554002387695 2315 12-13 -1.1920707075497674 2315 14-15 -0.003938999607242977 2315 16-17 -1.5990596979330078 2315 18-19 -2.7834467475406726 2315 20-21 -1.9959342995009806 2315 22-23 -1.2639928787792343 2315 24-25 -0.8867710516255798 2315 26-27 -0.3566575189627059 2315 28-29 -0.28043337540235314 2315 30-31 -2.5754272271343837 2315 32-33 -2.476041802303733 2315 34-35 -1.615184059297583 2315 36-37 -4.409831315134763 2315 38-39 -1.7684216169308797 2315 40-41 -1.419446753102612 2315 42-43 -1.6557274214955555 2315 44-45 -1.4193984212227164 2315 46-47 -1.3253447043202513 2315 48-49 -3.97032753607764 2315 50-51 -3.432349616081588 2315 52-53 -1.714359887911261 2315 54-55 -3.5153176740264236 2315 56-57 -5.595472411836653 2315 58-59 -6.1450225382136985 2315 60-61 -4.91063726332213 2315 62-63 -5.85838332715387 2315 64-65 -5.136085753782783 2315 66-67 -3.4930909629008973 2315 68-69 -1.476051862017318 2315 70-71 0.8661010264492823 2315 72-73 -0.5814100214035562 2315 74-75 -2.4434785509653487 2315 76-77 -4.489059027228691 2315 78-79 -3.4818768614116067 2315 80-81 -2.9885556497268695 2315 82-83 -6.316933013695628 2315 84-85 -4.182843626791225 2315 86-87 -4.698186171313729 2315 88-89 -3.2273513771876985 2315 90-91 -2.9408293546302637 2315 92-93 -4.101625464639419 2315 94-95 -2.237529393695432 2315 96-97 -0.9238836268315431 2315 98-99 -2.5491194584542356 2315 100-101 -1.7324189233054135 2316 1 -0.5459503391694192 2316 2 -0.3939341255175002 2316 3 -1.1595357729283045 2316 4 -0.6586322270737028 2316 5 -0.4331188664193064 2316 6 -2.665140160508898 2316 7 -0.6487713712161778 2316 8 -0.6818819324874568 2316 9 -0.16479790623154145 2316 10-11 -0.22858425026754503 2316 12-13 -1.1426271956251952 2316 14-15 -1.3174064555982454 2316 16-17 -1.5169352027252998 2316 18-19 -0.48897305921965284 2316 20-21 -4.951055996348046 2316 22-23 -4.544325173591158 2316 24-25 -3.0392132190393895 2316 26-27 -2.1261039626176057 2316 28-29 -1.5195586383869681 2316 30-31 -0.7371734330067383 2316 32-33 -2.286600357008737 2316 34-35 -2.7451804667263957 2316 36-37 -5.4024935785987935 2316 38-39 -2.6477346548403133 2316 40-41 -2.3304621290669836 2316 42-43 -3.867246958955022 2316 44-45 -1.683157428183172 2316 46-47 -1.575907320192691 2316 48-49 -1.0844379301430536 2316 50-51 -0.5495563499297376 2316 52-53 -3.22863104653349 2316 54-55 -1.4327852597825377 2316 56-57 -0.5493961844449196 2316 58-59 -0.41303796103770196 2316 60-61 -1.6209090130965436 2316 62-63 -1.2871140441181375 2316 64-65 -0.37527289550190446 2316 66-67 0.9208429265800788 2316 68-69 3.295643242834096 2316 70-71 3.525668259796994 2316 72-73 1.8252432041606497 2316 74-75 0.8939666142628901 2316 76-77 -0.6708221025657508 2316 78-79 -0.20468295989131136 2316 80-81 -0.4297287984412925 2316 82-83 -0.2702379989588799 2316 84-85 -0.130409509620538 2316 86-87 -0.5010699606472748 2316 88-89 -0.25668550192802186 2316 90-91 -0.2425825053392252 2316 92-93 0.07464842625936186 2316 94-95 0.9161803866290512 2316 96-97 0.8217503177996051 2316 98-99 -0.16493722498036334 2316 100-101 -0.2528437118062996 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 28.0 4 51.0 5 139.0 6 397.0 7 892.0 8 1764.0 9 3098.0 10 5149.0 11 7672.0 12 9776.0 13 12140.0 14 14954.0 15 18833.0 16 22914.0 17 25262.0 18 21570.0 19 16454.0 20 15662.0 21 15804.0 22 16678.0 23 18693.0 24 22541.0 25 27659.0 26 34367.0 27 42597.0 28 51696.0 29 61226.0 30 72453.0 31 83776.0 32 96523.0 33 111345.0 34 129816.0 35 147880.0 36 152152.0 37 130861.0 38 59918.0 39 4081.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.598753997602465 21.27685722877752 15.745850663715293 20.378538109904717 2 15.462697306044323 22.828080191849683 39.729718782031874 21.97950372007412 3 19.099522455308694 28.145895154331985 31.064635677400265 21.689946712959056 4 12.201723334342375 18.757958220159292 37.75273247956003 31.2875859659383 5 11.778696823571808 40.3975769224169 33.70679388395998 14.11693237005131 6 28.34269271505814 38.6069176367544 17.724959792755076 15.325429855432388 7 25.94762452919942 36.18150954094069 20.671569101890615 17.199296827969278 8 25.951262572700806 35.17864509650082 19.697053939829505 19.173038390968866 9 26.92310068388354 17.158591926151836 20.988010244181364 34.93029714578327 10-11 24.027801516583644 28.59670365801842 26.49672884975361 20.878765975644328 12-13 26.2274106671554 26.503833330930853 27.6672521857434 19.60150381617035 14-15 22.983923279840365 26.98130937990578 28.089848691711506 21.944918648542348 16-17 19.55694671107839 30.614196307374442 28.801106517780994 21.027750463766175 18-19 19.333419823738048 28.166629942566253 33.14144132827296 19.35850890542274 20-21 20.97318350085494 26.83293898314625 33.05512596897229 19.138751547026516 22-23 20.296953584742734 27.155969789137625 32.852768379498734 19.69430824662091 24-25 19.847792496981455 27.381288238068073 33.37815677496367 19.3927624899868 26-27 20.03845357856127 27.248980056925298 33.53636667223815 19.176199692275272 28-29 19.312867017692366 27.403449910527232 33.829458354229764 19.454224717550634 30-31 19.509862034146007 27.760629912611673 33.4541025080426 19.275405545199717 32-33 19.43204304212396 27.813587101092356 33.09321503041953 19.661154826364157 34-35 19.82099829064523 27.57954678071518 33.09352024109454 19.50593468754505 36-37 20.070126159751048 27.357516299900304 33.10417163387906 19.468185906469596 38-39 19.95666948734149 27.5585427452484 33.135920452160015 19.348867315250093 40-41 19.68885674180951 27.693090076918185 33.22834753894445 19.389705642327854 42-43 19.43850554908318 28.05613570250881 32.626128566672655 19.879230181735352 44-45 19.65948856636565 28.229475216074768 32.11067892722202 20.000357290337565 46-47 19.912971063187708 28.349920089488776 31.273284328882255 20.463824518441257 48-49 20.070877030242126 28.4101797692975 31.011775619057435 20.50716758140294 50-51 20.36140037578163 28.801851629812838 30.64372445016427 20.193023544241264 52-53 20.19787527091498 29.07172763899527 30.47924902882776 20.251148061261993 54-55 19.850007315208558 28.963726868658007 30.626030740360928 20.56023507577251 56-57 20.221626335594802 29.294400802043562 29.789463564699098 20.694509297662535 58-59 20.083028373061982 28.861181489492218 30.23491866906081 20.820871468384993 60-61 20.306892290794263 28.790553991899497 30.364900116702138 20.537653600604106 62-63 20.6156241290831 28.648103580592505 30.347652866080267 20.38861942424413 64-65 20.422061217548173 28.840083322149805 30.36607721748777 20.371778242814255 66-67 19.89297568945371 28.839622088714712 30.563282216730258 20.70412000510132 68-69 20.2236692709622 28.785869046510847 30.561789354167697 20.42867232835925 70-71 19.86153522164894 28.664418957787223 30.7286788625771 20.745366957986732 72-73 20.163773220452274 28.816714216728467 29.74806211555956 21.271450447259703 74-75 19.835584004707812 29.187620264773273 29.939784257285325 21.03701147323359 76-77 19.968468021610356 28.967130332520608 29.909554093338915 21.154847552530125 78-79 19.76445527708529 28.82797072990238 30.109833230189842 21.297740762822485 80-81 19.655959445498883 29.38660287409777 29.728103624026552 21.229334056376796 82-83 19.70127544313772 29.352284107859067 29.359251304375878 21.58718914462733 84-85 19.81333404721357 28.93789036035164 29.227080497547064 22.021695094887725 86-87 19.719375751420813 29.01597207897868 29.51476276982078 21.749889399779725 88-89 20.009390341676642 28.532017770260627 29.523838288431758 21.934753599630977 90-91 19.647140066255623 28.685417584786194 29.734201822922028 21.933240526036155 92-93 19.56217917828455 28.936763028158865 29.517691442786482 21.98336635077011 94-95 19.13700242207879 28.787030784583596 29.74299683488894 22.332969958448672 96-97 18.983661216736056 28.43145754166228 29.655679424912258 22.929201816689407 98-99 19.354761199445157 28.40140228411803 29.27053559138652 22.973300925050296 100-101 19.41167203202534 28.315227035504044 29.532618280158562 22.740482652312057 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5619.0 1 3732.5 2 1936.0 3 2176.0 4 2068.5 5 2021.0 6 3141.0 7 5568.5 8 7793.0 9 8555.0 10 7563.0 11 5966.5 12 5198.5 13 4766.0 14 4492.0 15 4362.0 16 4271.5 17 4325.5 18 4520.5 19 4735.5 20 5083.5 21 5621.0 22 6343.0 23 7327.5 24 8269.0 25 9539.5 26 11652.0 27 13956.0 28 16189.0 29 18599.5 30 21653.5 31 25216.5 32 29241.5 33 33596.0 34 37509.5 35 40899.5 36 45263.5 37 48638.5 38 48818.5 39 48590.0 40 48421.5 41 47781.5 42 46610.0 43 46482.0 44 47588.0 45 48123.5 46 48604.0 47 50924.5 48 58087.5 49 56425.5 50 46385.0 51 40328.5 52 36192.0 53 33946.0 54 32094.0 55 29653.5 56 26832.5 57 23947.5 58 21450.5 59 19483.5 60 17066.0 61 14335.0 62 11625.5 63 8702.5 64 6134.5 65 4419.0 66 3287.5 67 2467.5 68 1764.5 69 1267.5 70 917.5 71 719.0 72 630.0 73 592.0 74 477.5 75 353.5 76 256.5 77 159.0 78 107.5 79 71.0 80 40.5 81 24.5 82 15.0 83 8.0 84 7.0 85 5.0 86 2.0 87 1.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022583326640706138 2 0.018533429158026315 3 0.0 4 0.0 5 1.3728466042982454E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.0889048596710524E-4 18-19 0.001235561943868421 20-21 0.0 22-23 0.0 24-25 0.0 26-27 3.775328161820175E-4 28-29 0.016371195756256576 30-31 0.003672364666497806 32-33 0.007859546809607454 34-35 0.00940399923944298 36-37 0.0995313788116228 38-39 0.10306645881769078 40-41 0.11734406350239254 42-43 0.10272324716661621 44-45 0.09802124754689473 46-47 0.09946273648140788 48-49 0.10224275085511184 50-51 0.10025212327887938 52-53 0.07633027119898245 54-55 0.06562206768545613 56-57 0.03754735462755701 58-59 0.014861064491528507 60-61 0.008717575937293859 62-63 0.001990627576232456 64-65 0.004907926610366227 66-67 0.42709257859718414 68-69 0.8216830138376074 70-71 0.9117760722446797 72-73 0.2846940645663486 74-75 0.1526605423979649 76-77 0.014449210510239034 78-79 0.008134116130467104 80-81 0.00151013126472807 82-83 0.0 84-85 0.0 86-87 2.4024815575219297E-4 88-89 7.55065632364035E-4 90-91 0.003088904859671052 92-93 0.0014414889345131577 94-95 0.22813278446926094 96-97 0.2343449153537105 98-99 0.013968714198734647 100-101 0.007241765837673246 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1456827.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.63699072161762 #Duplication Level Percentage of deduplicated Percentage of total 1 71.79300840628459 34.20002875327195 2 14.059518179382113 13.395062741232802 3 5.395102772076461 7.710193820867397 4 2.871305791339474 5.471214693638618 5 1.6810894393091549 4.0041021011289795 6 1.0135331632925155 2.8969001937490395 7 0.6820151924429757 2.27424059760858 8 0.4785035091811348 1.8235573781698555 9 0.3394185825929432 1.4551991882752175 >10 1.53941668623375 12.697853348289348 >50 0.0904407508090143 2.981421265942803 >100 0.05036962259529703 4.334102007983502 >500 0.0033579488975526367 1.060969202246606 >1k 0.002335964450471399 2.369880404231602 >5k 1.4599777815446245E-4 0.5337186272068472 >10k+ 4.379933344633874E-4 2.7915556761569498 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15830 1.0866080873020612 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14137 0.9703966222482149 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10171 0.6981611406158726 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7674 0.5267612420692368 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4085 0.28040391892791666 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3475 0.23853209749682014 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3393 0.2329034264191973 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3393 0.2329034264191973 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2572 0.17654807331275435 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2256 0.15485709696484207 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1873 0.12856708449253068 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1755 0.12046728952717105 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1632 0.11202428291073682 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1619 0.11113193261794298 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1579 0.10838623940934648 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1558 0.10694475047483333 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.029634953223684E-4 0.0 12-13 0.0 0.0 0.0 1.3728466042982454E-4 0.0 14-15 0.0 0.0 0.0 1.3728466042982454E-4 0.0 16-17 0.0 0.0 0.0 2.745693208596491E-4 0.0 18-19 0.0 0.0 0.0 3.0889048596710524E-4 0.0 20-21 0.0 0.0 0.0 5.834598068267543E-4 0.0 22-23 0.0 0.0 0.0 0.001269883108975877 0.0 24-25 0.0 0.0 0.0 0.002299518062199561 0.0 26-27 0.0 0.0 0.0 0.0038782916571425434 0.0 28-29 0.0 0.0 0.0 0.00693287535170614 0.0 30-31 0.0 0.0 0.0 0.01757243653501754 0.0 32-33 0.0 0.0 0.0 0.03641475617901096 0.0 34-35 0.0 0.0 0.0 0.06325390729304166 3.4321165107456135E-5 36-37 0.0 0.0 0.0 0.10519437105435306 6.864233021491227E-5 38-39 0.0 0.0 0.0 0.16601147562476531 6.864233021491227E-5 40-41 0.0 0.0 0.0 0.2383261705061754 6.864233021491227E-5 42-43 0.0 0.0 0.0 0.3056299752818969 6.864233021491227E-5 44-45 0.0 0.0 0.0 0.3830585237643179 6.864233021491227E-5 46-47 0.0 0.0 0.0 0.4739752901339692 6.864233021491227E-5 48-49 0.0 0.0 0.0 0.5790666976929999 6.864233021491227E-5 50-51 0.0 0.0 0.0 0.6921549367220678 6.864233021491227E-5 52-53 0.0 0.0 0.0 0.7999920374896952 6.864233021491227E-5 54-55 0.0 0.0 0.0 0.9158946120575744 6.864233021491227E-5 56-57 0.0 0.0 0.0 1.0483743093723552 6.864233021491227E-5 58-59 0.0 3.4321165107456135E-5 0.0 1.1853157581511051 6.864233021491227E-5 60-61 0.0 6.864233021491227E-5 0.0 1.3287439071351643 6.864233021491227E-5 62-63 0.0 6.864233021491227E-5 0.0 1.4664747427113856 6.864233021491227E-5 64-65 0.0 6.864233021491227E-5 0.0 1.600636177116432 6.864233021491227E-5 66-67 0.0 6.864233021491227E-5 0.0 1.7343857575401884 6.864233021491227E-5 68-69 0.0 6.864233021491227E-5 0.0 1.9035547803548396 6.864233021491227E-5 70-71 0.0 6.864233021491227E-5 0.0 2.1269169228741642 6.864233021491227E-5 72-73 0.0 6.864233021491227E-5 0.0 2.372862392034195 6.864233021491227E-5 74-75 0.0 6.864233021491227E-5 0.0 2.629756312863504 6.864233021491227E-5 76-77 0.0 6.864233021491227E-5 0.0 2.9052523051810546 6.864233021491227E-5 78-79 0.0 6.864233021491227E-5 0.0 3.225503096798728 6.864233021491227E-5 80-81 0.0 6.864233021491227E-5 0.0 3.58529187062019 6.864233021491227E-5 82-83 0.0 6.864233021491227E-5 0.0 3.9460073158995543 6.864233021491227E-5 84-85 0.0 6.864233021491227E-5 0.0 4.314307738667666 6.864233021491227E-5 86-87 0.0 6.864233021491227E-5 0.0 4.68878597115512 6.864233021491227E-5 88-89 0.0 6.864233021491227E-5 0.0 5.078777370271144 6.864233021491227E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCGTC 145 2.4672772E-8 29.400188 7 ATGGGCG 250 0.0 26.525503 5 GGTATCA 14070 0.0 26.304277 1 GTATCAA 19305 0.0 25.995344 1 ATACCGT 110 9.660776E-5 25.836527 6 GAGTACT 11685 0.0 23.4504 12-13 GTACATG 11905 0.0 22.768585 1 TACATGG 11945 0.0 22.2117 2 GTACTTT 12410 0.0 21.908655 14-15 TATCAAC 23325 0.0 21.449749 2 CATTCCG 265 8.221832E-10 21.449194 9 ATCAACG 23530 0.0 21.197374 3 TCAACGC 23695 0.0 21.049765 4 AGTACTT 11945 0.0 21.016739 12-13 ACATGGG 12035 0.0 20.977644 3 CAACGCA 23965 0.0 20.753313 5 AACGCAG 24245 0.0 20.494102 6 ACTTTTT 14025 0.0 19.943096 16-17 GCACCGT 215 1.1086177E-6 19.828033 6 CATGGGG 2865 0.0 19.508907 4 >>END_MODULE