FastQCFastQC Report
Fri 27 May 2016
SRR939436_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939436_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1445714
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116030.8025792099958913No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83480.577430944156313No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65570.45354752046393687No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA52940.36618584311973185No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA29080.20114628481151872No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA22970.15888343060937365No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21080.14581030549610782No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19560.13529646942617973No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17200.1189723555281335No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14490.10022729253503805No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCGG2201.8189894E-1228.6449766
GTATCAA175700.028.2619151
GTACATG112900.028.0454881
ACGCGGA2103.274181E-1127.7006387
TACATGG114300.026.8642332
ACATGGG116250.025.2450623
GAGTACT98000.024.7478812-13
GGTATCA125400.024.568541
TATCAAC208100.023.5662162
AGTACTT100600.023.43926212-13
CATGGGA87150.023.4185624
CAACGCG2502.928573E-1023.2916955
GTACTTT106650.023.09172214-15
ATCAACG216150.022.8811263
TCAACGC219250.022.7062154
CAACGCA220250.022.4280625
AACGCAG221250.022.129216
ACTTTTT128800.021.04794916-17
ATGGGAG26900.020.9250075
AGTACAT82350.020.7809522
Produced by FastQC (version 0.11.5)