FastQCFastQC Report
Fri 27 May 2016
SRR939436_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939436_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1445714
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157791.0914330220223363No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137760.9528855638113762No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101430.7015910477452664No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA78950.5460969458689616No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA38270.26471349105009706No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34040.23545459198707352No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA33570.2322036032022931No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32510.22487158594300116No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25890.1790810630594986No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT21460.14843876451358984No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG17430.12056326493345157No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT16220.11219369806199565No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16110.11143282834640876No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA15680.10845851945820544No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15440.10679844007874309No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT14830.10257907165594302No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14470.10008895258674952No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA131300.027.0448781
GTATCAA180000.025.582761
GTACATG118450.024.2876631
TACATGG118600.023.6107732
AGTACTT120950.022.55960712-13
GAGTACT117900.022.43946312-13
ACATGGG120050.022.096193
CCGTCCG550.002714172721.81058566-67
CTGGTCG3500.021.6764959
CGACCGT550.00290881521.55184418-19
GTACTTT126150.020.87799814-15
TATCAAC222450.020.6104562
CATGGGG31050.020.3283824
ATCAACG225400.020.3161373
TCAACGC226450.020.1402054
CAACGCA228550.019.9378935
AACGCAG231500.019.7657576
CATGGGA86400.019.6644634
TACTTTT137100.019.6600714-15
CGCATAG1352.0496373E-819.54874666-67
Produced by FastQC (version 0.11.5)