##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939435_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 963800 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.94853185308155 31.0 31.0 31.0 30.0 34.0 2 31.16724112886491 31.0 31.0 34.0 28.0 34.0 3 31.15970948329529 31.0 31.0 34.0 28.0 34.0 4 35.02949678356506 35.0 35.0 37.0 32.0 37.0 5 34.7107719443868 35.0 35.0 37.0 32.0 37.0 6 34.69985992944594 35.0 35.0 37.0 32.0 37.0 7 34.60112575223075 35.0 35.0 37.0 30.0 37.0 8 34.664038182195476 35.0 35.0 37.0 31.0 37.0 9 35.915275990869475 37.0 35.0 39.0 30.0 39.0 10-11 35.7884493670886 37.0 35.0 39.0 30.0 39.0 12-13 35.77621135090267 37.0 35.0 39.0 30.0 39.0 14-15 36.35281023033825 38.0 35.0 40.0 30.0 41.0 16-17 36.411318219547624 38.0 34.5 40.0 30.5 41.0 18-19 36.611367503631456 38.0 35.0 40.0 30.0 41.0 20-21 36.554277858476866 38.0 35.0 40.0 30.0 41.0 22-23 36.43085598671924 38.0 35.0 40.0 30.0 41.0 24-25 36.1861091512762 38.0 34.0 40.0 29.5 41.0 26-27 35.59412689354638 38.0 34.0 40.0 28.0 41.0 28-29 35.33226706785641 38.0 34.0 40.0 27.0 41.0 30-31 35.18045133845196 37.0 34.0 40.0 27.0 41.0 32-33 34.984336480597634 37.0 33.0 40.0 27.0 41.0 34-35 34.95594210417099 37.0 33.0 40.0 27.0 41.0 36-37 34.78670471052085 37.0 33.0 40.0 25.5 41.0 38-39 34.56904544511309 37.0 33.0 40.0 25.0 41.0 40-41 34.348722764058934 37.0 33.0 40.0 24.5 41.0 42-43 34.15313913675037 37.0 33.0 40.0 24.0 41.0 44-45 33.94854170989832 37.0 33.0 40.0 24.0 41.0 46-47 33.46891056235734 37.0 33.0 40.0 21.5 41.0 48-49 33.0783015148371 37.0 32.0 40.0 18.5 41.0 50-51 32.83383533928201 37.0 31.5 40.0 16.0 41.0 52-53 32.270868437435155 37.0 31.0 40.0 13.0 41.0 54-55 32.160812409213534 37.0 31.0 39.0 12.5 41.0 56-57 31.923695787507782 36.0 31.0 39.0 8.5 40.0 58-59 31.593490869474994 36.0 30.0 39.0 8.0 40.0 60-61 31.26265978418759 36.0 29.5 39.0 7.5 40.0 62-63 30.804437123884625 35.0 28.5 39.0 4.0 40.0 64-65 30.169098360655738 35.0 28.0 38.0 2.0 40.0 66-67 28.527324652417512 33.5 25.0 37.5 2.0 39.5 68-69 25.14454814276821 30.0 14.0 36.0 2.0 39.0 70-71 24.489915957667563 29.5 10.5 35.0 2.0 38.0 72-73 26.11591201494086 31.0 21.0 35.0 2.0 38.0 74-75 26.62460987756796 31.0 22.0 35.0 2.0 37.0 76-77 26.41425191948537 31.0 24.0 34.0 2.0 36.0 78-79 27.128484644117037 32.0 25.5 35.0 2.0 37.0 80-81 27.060010375596597 32.0 26.0 35.0 2.0 36.5 82-83 26.712515044615067 32.0 25.0 35.0 2.0 36.0 84-85 26.392434633741438 32.0 24.5 35.0 2.0 36.0 86-87 25.887814899356712 32.0 23.5 34.5 2.0 35.0 88-89 25.205016082174726 31.5 20.0 34.0 2.0 35.0 90-91 24.65387528532891 31.0 18.5 34.0 2.0 35.0 92-93 24.280194542436192 31.0 14.5 34.0 2.0 35.0 94-95 23.076608736252332 30.5 2.0 34.0 2.0 35.0 96-97 22.187807117659265 29.0 2.0 34.0 2.0 35.0 98-99 21.83392975721104 29.5 2.0 34.0 2.0 35.0 100-101 20.602709587051258 28.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5576733525711148 1101 2 0.36516282206199335 1101 3 0.5317434848206659 1101 4 0.36999916793411103 1101 5 0.42538671162900243 1101 6 0.616070885786641 1101 7 0.6401210397958295 1101 8 0.5987062105257337 1101 9 0.8142286445056541 1101 10-11 0.8297500003015585 1101 12-13 0.901066314688812 1101 14-15 1.0143104526566233 1101 16-17 1.053930368568679 1101 18-19 0.9938719559509153 1101 20-21 0.9366188760224787 1101 22-23 1.073812123780307 1101 24-25 0.9243253631556172 1101 26-27 1.012697848676737 1101 28-29 0.9266791922482227 1101 30-31 1.0812927545738944 1101 32-33 0.9510233308672511 1101 34-35 0.8506285633022799 1101 36-37 0.8171113482528867 1101 38-39 0.7618857834193875 1101 40-41 1.0722429589592082 1101 42-43 1.1471839592568216 1101 44-45 0.8849812953271581 1101 46-47 0.9115386048159948 1101 48-49 0.9189933784775519 1101 50-51 0.8337000911720196 1101 52-53 0.9959797720307577 1101 54-55 0.8444011123250448 1101 56-57 0.6933366841445086 1101 58-59 0.5896387471336801 1101 60-61 0.4838518604809643 1101 62-63 0.7273106491297057 1101 64-65 0.35495781327702147 1101 66-67 -2.203812098523027 1101 68-69 -7.545022848357547 1101 70-71 -10.517175905602937 1101 72-73 -10.163675819935381 1101 74-75 -8.33170677639895 1101 76-77 -3.6126825592964664 1101 78-79 -1.0735475705359576 1101 80-81 -0.6254791752345312 1101 82-83 -0.09621661158845285 1101 84-85 -0.2933429115175521 1101 86-87 -0.012743722367662969 1101 88-89 0.11414734291163242 1101 90-91 -0.12646093279699855 1101 92-93 -0.4879736021715573 1101 94-95 -3.3100953547852434 1101 96-97 -0.6097013881331108 1101 98-99 -0.41582614867768797 1101 100-101 -0.1862687182768532 1102 1 0.3590701430391299 1102 2 0.32396354125478055 1102 3 0.3950431842624873 1102 4 0.2497691872557084 1102 5 0.3729366257552371 1102 6 0.2752191253744485 1102 7 0.34476895735701873 1102 8 0.3587024535484886 1102 9 0.6346652589324435 1102 10-11 0.5645040524698715 1102 12-13 0.564867168059358 1102 14-15 0.5730299943053936 1102 16-17 0.5241831594660553 1102 18-19 0.6267314181664503 1102 20-21 0.9062947025574744 1102 22-23 0.8245635192290024 1102 24-25 0.61757488011569 1102 26-27 0.618497011407527 1102 28-29 0.48328207975358595 1102 30-31 0.22507093190321825 1102 32-33 0.23636239806347703 1102 34-35 0.4832364422202744 1102 36-37 0.8422898046719567 1102 38-39 0.9949928035997289 1102 40-41 0.9869126133173012 1102 42-43 0.9341264506694458 1102 44-45 0.7189564456062456 1102 46-47 0.8230450185128575 1102 48-49 0.7864961962104857 1102 50-51 0.8187459799654988 1102 52-53 0.815171887745656 1102 54-55 0.6640326601996662 1102 56-57 0.7565914340371656 1102 58-59 0.7383311848751966 1102 60-61 0.7956373639159153 1102 62-63 0.7740749524787809 1102 64-65 0.7755279345737129 1102 66-67 -2.260042642748015 1102 68-69 -7.271870476094772 1102 70-71 -10.894846043000962 1102 72-73 -8.770045238140618 1102 74-75 -6.89292202435945 1102 76-77 -3.287452444440305 1102 78-79 -0.42904818775364717 1102 80-81 -0.017710953894898296 1102 82-83 0.09454390794782697 1102 84-85 0.19140107731885792 1102 86-87 0.244495167713918 1102 88-89 0.34117611062309194 1102 90-91 0.3838506279586902 1102 92-93 -0.349421648543391 1102 94-95 -3.6224511136946447 1102 96-97 -0.5682438151404092 1102 98-99 0.20271990112909677 1102 100-101 0.36334015355655325 1103 1 0.42234531246783646 1103 2 0.20510119546137773 1103 3 0.20511757405440534 1103 4 0.29800921346421205 1103 5 0.5624237729993737 1103 6 0.5269283036452137 1103 7 0.5815191029598097 1103 8 0.34351508922382124 1103 9 0.7480128988434913 1103 10-11 0.7678478703075342 1103 12-13 0.7652956208311039 1103 14-15 0.8518663797821802 1103 16-17 0.8699715986040886 1103 18-19 0.7872150676343423 1103 20-21 0.9486452611427438 1103 22-23 0.8260890394280764 1103 24-25 0.8289347109517138 1103 26-27 0.9040522213402795 1103 28-29 0.8898026031294606 1103 30-31 1.150885480269821 1103 32-33 0.9901509662585326 1103 34-35 0.8984840331558388 1103 36-37 0.7983880799406577 1103 38-39 0.7880101568056332 1103 40-41 1.023648364098264 1103 42-43 0.9349988265099682 1103 44-45 0.9223379050757288 1103 46-47 1.071160041412206 1103 48-49 0.7045305088329243 1103 50-51 0.9035753268707793 1103 52-53 0.8047989294939484 1103 54-55 1.178684768792884 1103 56-57 0.9983189615693284 1103 58-59 0.8105927768432188 1103 60-61 0.5069909900873562 1103 62-63 0.5699419013060165 1103 64-65 0.27960853564852783 1103 66-67 -2.664918930009094 1103 68-69 -7.318632964843648 1103 70-71 -10.864095725322136 1103 72-73 -7.984507401268697 1103 74-75 -6.100773737839621 1103 76-77 -2.9244799799144374 1103 78-79 -0.5203556181290381 1103 80-81 0.16786270457388852 1103 82-83 0.46046524272836464 1103 84-85 0.3211910783777867 1103 86-87 0.32798099791458313 1103 88-89 0.37583619427851644 1103 90-91 0.6305389916479989 1103 92-93 0.6031978781151572 1103 94-95 1.4315952376316652 1103 96-97 1.7232658045715645 1103 98-99 1.407736910182944 1103 100-101 1.1935561255213933 1104 1 0.374424912442354 1104 2 0.15996192654355923 1104 3 0.3466685712593893 1104 4 0.2553055783094038 1104 5 0.46830800933589245 1104 6 0.4331171983967579 1104 7 0.46476468704635465 1104 8 0.3088632574344743 1104 9 0.48734454525677506 1104 10-11 0.6978903754654908 1104 12-13 0.7150077880550825 1104 14-15 0.8135026976789419 1104 16-17 0.840816686742663 1104 18-19 0.6394814648729792 1104 20-21 0.6945540171726137 1104 22-23 0.697688585952605 1104 24-25 0.8197957752795588 1104 26-27 0.930953641064832 1104 28-29 0.8148933880700255 1104 30-31 0.7935677158255032 1104 32-33 0.5964755279342953 1104 34-35 0.8707437818167278 1104 36-37 0.901575395042407 1104 38-39 0.7486459209265064 1104 40-41 0.7541890225198173 1104 42-43 1.0311267242362163 1104 44-45 1.03616212778838 1104 46-47 0.9073060879587871 1104 48-49 0.8423079889402842 1104 50-51 1.0573412479274893 1104 52-53 0.9488284413380583 1104 54-55 1.1067020714305826 1104 56-57 1.1376507713383326 1104 58-59 1.136636905757019 1104 60-61 0.8841086927832116 1104 62-63 0.8951557747804806 1104 64-65 0.6651345655654914 1104 66-67 -2.042392748171313 1104 68-69 -6.869335312755389 1104 70-71 -8.266333045242877 1104 72-73 -6.941423147289761 1104 74-75 -5.994296030177747 1104 76-77 -2.9314328076836063 1104 78-79 -0.25200627865788405 1104 80-81 0.6023439929931129 1104 82-83 0.7813599910053703 1104 84-85 0.5866016484729109 1104 86-87 1.0414468998586806 1104 88-89 0.7552494532087657 1104 90-91 0.6237303253004214 1104 92-93 0.7541467298061342 1104 94-95 1.6663178024471286 1104 96-97 1.777023312808776 1104 98-99 1.2655635276904285 1104 100-101 1.0368657287140195 1105 1 0.4124103097509746 1105 2 0.2736797583867947 1105 3 0.33565717179298105 1105 4 0.22104612545033575 1105 5 0.5251882522122742 1105 6 0.6344679055316718 1105 7 0.5712530994009342 1105 8 0.43149826542162373 1105 9 0.4794343359180786 1105 10-11 0.6542357717704945 1105 12-13 0.7618806710411903 1105 14-15 0.8366901156119866 1105 16-17 0.9661982719626678 1105 18-19 0.7644436199826643 1105 20-21 0.9247617668025327 1105 22-23 0.9701822505925151 1105 24-25 0.765202116494109 1105 26-27 0.9063849891792728 1105 28-29 0.8549537726242846 1105 30-31 1.0549951999975562 1105 32-33 0.9363272428723661 1105 34-35 0.9524201640961181 1105 36-37 0.8985440402371481 1105 38-39 0.8120384121657338 1105 40-41 1.0899003810050232 1105 42-43 1.1058392285225196 1105 44-45 1.1670682306428475 1105 46-47 1.1664244065583986 1105 48-49 1.0259949594254394 1105 50-51 1.0304776484393372 1105 52-53 0.9151147076155368 1105 54-55 0.9992225829429557 1105 56-57 0.7567045236278425 1105 58-59 1.034229887098025 1105 60-61 1.0476132550788329 1105 62-63 1.1131603245308277 1105 64-65 1.1770065703828756 1105 66-67 2.0407769391169914 1105 68-69 -4.790289254896617 1105 70-71 -4.516364408411643 1105 72-73 -0.8451884002441687 1105 74-75 1.2401683287172638 1105 76-77 1.23084900920567 1105 78-79 1.3474626383509865 1105 80-81 1.2837109253406638 1105 82-83 1.0897306116615724 1105 84-85 1.1799769108780893 1105 86-87 1.368608240362331 1105 88-89 1.3692257175626459 1105 90-91 1.4533672652587697 1105 92-93 1.3176358196051616 1105 94-95 1.9992625112714713 1105 96-97 2.09769080467526 1105 98-99 1.8434202941895457 1105 100-101 1.4798855418731698 1106 1 0.11366573127442336 1106 2 0.13830177654890008 1106 3 0.15882994896836777 1106 4 -0.06456904803841468 1106 5 0.15326750810817913 1106 6 0.18974118419797747 1106 7 0.1517542514746637 1106 8 0.15925392413672768 1106 9 0.3304373177559725 1106 10-11 0.43858493482281347 1106 12-13 0.34749584452993787 1106 14-15 0.3905851413642196 1106 16-17 0.31738168887782336 1106 18-19 0.32663950640175443 1106 20-21 0.3966439672633584 1106 22-23 0.44404881334664026 1106 24-25 0.4241006154098059 1106 26-27 0.5091220114075199 1106 28-29 0.616554138577122 1106 30-31 0.49850843190322536 1106 32-33 0.441325633357593 1106 34-35 0.7369129128085063 1106 36-37 0.8841096576131307 1106 38-39 0.6696251565409028 1106 40-41 0.8959199662584751 1106 42-43 0.9281521859635617 1106 44-45 0.8954270338415427 1106 46-47 0.8326957538069735 1106 48-49 0.7244557550340076 1106 50-51 0.8582680387890278 1106 52-53 1.061954975980946 1106 54-55 1.1507054543173112 1106 56-57 1.2202862869783395 1106 58-59 1.2295995672281386 1106 60-61 1.2211888345041508 1106 62-63 0.9840933348317229 1106 64-65 0.9322374933972419 1106 66-67 1.5368323572519849 1106 68-69 -4.685013858447714 1106 70-71 -5.383816631236254 1106 72-73 -1.547159208728857 1106 74-75 0.9577213579934885 1106 76-77 0.941407849677347 1106 78-79 1.0681944593051753 1106 80-81 1.0742008108109822 1106 82-83 0.974139496183124 1106 84-85 1.003441518495329 1106 86-87 0.985303991243331 1106 88-89 0.6941172870936825 1106 90-91 0.43440209854692213 1106 92-93 0.39506364557425755 1106 94-95 1.5167952098347683 1106 96-97 1.6390172142713553 1106 98-99 1.3125544599526258 1106 100-101 1.5126967712036112 1107 1 0.049751436823182615 1107 2 0.05025934076551053 1107 3 0.037445608370124006 1107 4 -0.0032036110318500732 1107 5 0.15831483658588752 1107 6 0.00191975364087682 1107 7 0.018070414045553207 1107 8 0.08916990045163686 1107 9 0.1476562658266971 1107 10-11 0.11657319652636033 1107 12-13 0.04558220336459584 1107 14-15 0.17294711734553658 1107 16-17 -0.11961270954316916 1107 18-19 0.21810891316255976 1107 20-21 0.2218595863559898 1107 22-23 0.18700514048997263 1107 24-25 0.3791585424085895 1107 26-27 0.43095878723316616 1107 28-29 0.3888944310268059 1107 30-31 0.4946156472373673 1107 32-33 0.3470604757349989 1107 34-35 0.2461318886786259 1107 36-37 0.23393295767984768 1107 38-39 0.2917270691395899 1107 40-41 0.41878149744940174 1107 42-43 0.4781563558734945 1107 44-45 0.04174743582085938 1107 46-47 -0.041164470812169895 1107 48-49 0.30451281330154956 1107 50-51 0.16353671998551533 1107 52-53 0.374604397753437 1107 54-55 0.2583716599772714 1107 56-57 0.3428173528073195 1107 58-59 -0.08208382541057802 1107 60-61 0.006354206182120237 1107 62-63 0.1422945051864417 1107 64-65 0.07188256456370468 1107 66-67 0.7630126155084049 1107 68-69 -5.393178368344298 1107 70-71 -5.470908675048111 1107 72-73 -2.097364055554163 1107 74-75 0.18130324112481588 1107 76-77 0.11148023236387772 1107 78-79 -0.0725808311985503 1107 80-81 -0.3438665401842371 1107 82-83 -0.20812552939232276 1107 84-85 -0.18615299251323236 1107 86-87 0.17608953277563089 1107 88-89 -0.008220642407042789 1107 90-91 -0.09322355473896593 1107 92-93 -0.2047396736740481 1107 94-95 0.5586385185192988 1107 96-97 0.7520686848595908 1107 98-99 0.507634139981537 1107 100-101 0.17526592220994885 1108 1 -0.04237340732273864 1108 2 -0.15673498815698395 1108 3 0.019145322805229625 1108 4 -0.023173043521893533 1108 5 -0.0050001294278700925 1108 6 0.012960255591565328 1108 7 0.08795337265875247 1108 8 -0.021536772114025382 1108 9 0.1385262332481645 1108 10-11 0.17135339199754185 1108 12-13 0.13218679653516574 1108 14-15 0.16923678472277004 1108 16-17 0.03069095453598436 1108 18-19 0.17296150536784438 1108 20-21 0.19715420084337154 1108 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2316 94-95 1.4948704579480712 2316 96-97 1.404968509112667 2316 98-99 0.4314848913079423 2316 100-101 0.3249431658071451 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 4.0 4 18.0 5 63.0 6 224.0 7 535.0 8 959.0 9 1748.0 10 3017.0 11 4459.0 12 6054.0 13 7759.0 14 9465.0 15 12138.0 16 14844.0 17 17026.0 18 14611.0 19 11685.0 20 11014.0 21 11032.0 22 11646.0 23 13023.0 24 15061.0 25 18028.0 26 22559.0 27 28101.0 28 33953.0 29 40404.0 30 47630.0 31 55196.0 32 63291.0 33 74037.0 34 84897.0 35 98515.0 36 101739.0 37 87210.0 38 39160.0 39 2695.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.023059743623804 21.34136722901169 15.279415183315622 18.356157844048884 2 13.580696614684678 24.643447560873096 39.29771908140593 22.47813674303629 3 21.647748495538494 30.172027391575018 32.82444490558207 15.35577920730442 4 10.864702220377671 18.407553434322473 41.57418551566715 29.153558829632704 5 9.778283646278945 43.3454485841965 35.15867935119252 11.717588418332038 6 23.331396555301932 40.750466901846856 18.85743930275991 17.060697240091304 7 22.684374351525214 39.291865532268105 21.199107698692675 16.82465241751401 8 22.409628553641834 32.67026354015356 21.49377464204192 23.42633326416269 9 24.638202946669434 20.96876945424362 23.704918032786885 30.688109566300064 10-11 21.721778377256694 27.234851628968666 28.342342809711557 22.701027184063083 12-13 28.817493255862214 26.261880058103344 26.91019921145466 18.01042747457979 14-15 25.264370201286575 26.960832122847062 30.33886698485163 17.435930691014732 16-17 17.154563828860052 30.924594001220175 32.60804153576068 19.312800634159096 18-19 16.43533085387687 32.80337502133512 33.98735093301266 16.773943191775356 20-21 18.502542021166217 30.174050632911392 30.768261050010377 20.555146295912017 22-23 17.899201079062045 29.080670263540153 29.817960157709066 23.20216849968873 24-25 19.42322058518365 29.69464619215605 32.1279311060386 18.754202116621705 26-27 21.533142836984513 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17.817630783036424 52-53 18.00829043951582 30.596124551808497 32.92599349765378 18.469591511021903 54-55 16.075894956844593 29.400392671108357 33.80890975105565 20.714802620991396 56-57 19.200163581437035 32.418965689935334 28.67190244899887 19.708968279628767 58-59 18.140531330130948 29.692411304022077 30.492021538784027 21.67503582706295 60-61 18.431685880607237 28.428469145282293 31.591298212117753 21.548546761992714 62-63 22.50204014084105 27.742128272850223 30.277194571591178 19.47863701471755 64-65 20.90386042095761 28.58641459707083 32.2521802740323 18.257544707939257 66-67 17.72998176608492 29.127064339671787 32.52841885907788 20.614535035165407 68-69 17.7731760757757 31.775141708070652 30.88401671048977 19.56766550566388 70-71 17.963464353244927 31.27794145669349 29.426869028249797 21.33172516181179 72-73 18.3318653384825 29.169101040880317 28.462850712467414 24.036182908169767 74-75 18.692892095481454 30.85180339657267 29.750561921516994 20.704742586428882 76-77 20.664912936097792 28.19760081356495 30.496388767822673 20.64109748251458 78-79 18.303572586666057 28.343434018512763 32.411084829671886 20.94190856514929 80-81 17.89884782454187 32.13111216136786 30.267938249745406 19.702101764344867 82-83 17.500051877982983 31.726654907657192 28.85038389707408 21.922909317285743 84-85 18.042799335961817 28.86454658642872 28.907760946254406 24.184893131355054 86-87 19.565033562513324 29.477997735003736 29.73162958854989 21.225339113933046 88-89 21.299405630557136 26.782887845904913 30.74363881512086 21.174067708417095 90-91 18.139290928201525 27.934500766264048 32.70629726894571 21.21991103658872 92-93 17.553410871896396 31.042350498584476 30.748195773198926 20.6560428563202 94-95 17.32705700793363 31.199673877889317 29.035527135418672 22.437741978758385 96-97 17.129158736596885 29.41951889176625 27.759248281384874 25.692074090251992 98-99 18.678852719291875 28.93225896831903 28.14723920014113 24.241649112247966 100-101 19.986116356965272 29.316899859295813 28.957045793193902 21.73993799054501 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3298.0 1 2176.5 2 1069.5 3 1084.0 4 971.5 5 983.0 6 1646.5 7 2790.0 8 3612.5 9 3798.0 10 3470.0 11 2913.5 12 2585.0 13 2485.5 14 2385.0 15 2340.5 16 2319.0 17 2374.5 18 2646.5 19 2926.5 20 3231.0 21 3624.5 22 4131.5 23 4641.0 24 5172.5 25 6282.5 26 8385.5 27 10704.5 28 11745.0 29 12748.5 30 14601.0 31 16524.0 32 20615.5 33 26382.0 34 31820.5 35 35338.5 36 39267.5 37 43315.5 38 40865.0 39 34880.0 40 30767.0 41 27563.0 42 24607.5 43 23939.5 44 25358.0 45 26671.0 46 32369.0 47 52015.5 48 100427.0 49 97140.0 50 46559.0 51 24418.5 52 11094.5 53 5026.5 54 2902.0 55 2185.0 56 1842.5 57 1527.5 58 1193.5 59 944.0 60 779.0 61 612.0 62 444.0 63 317.5 64 272.0 65 259.5 66 250.0 67 223.0 68 205.5 69 204.5 70 183.0 71 171.0 72 157.0 73 133.0 74 119.5 75 102.0 76 80.5 77 63.5 78 48.0 79 33.0 80 32.5 81 25.0 82 11.0 83 8.0 84 6.0 85 4.0 86 2.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022411288649097322 2 0.020439925295704504 3 0.0 4 0.0 5 1.0375596596804317E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 4.150238638721727E-4 18-19 0.0014007055405685827 20-21 0.0 22-23 0.0 24-25 0.0 26-27 2.0751193193608634E-4 28-29 0.017171612367711146 30-31 0.004772774434529985 32-33 0.008871135090267691 34-35 0.009856816766964099 36-37 0.10391159991699522 38-39 0.10583108528740404 40-41 0.11957875077816973 42-43 0.10541606142353185 44-45 0.09996887321020959 46-47 0.10152521269973024 48-49 0.1038078439510272 50-51 0.10209587051255448 52-53 0.07916580203361694 54-55 0.06775264577713218 56-57 0.03792280556131977 58-59 0.014837103133430172 60-61 0.009338036937123885 62-63 0.0019194853704087985 64-65 0.004669018468561942 66-67 0.4202116621705748 68-69 0.8169225980493879 70-71 0.9003942726706786 72-73 0.2841357128034862 74-75 0.15324756173479975 76-77 0.014525835235526042 78-79 0.008974891056235734 80-81 0.001504461506536626 82-83 0.0 84-85 0.0 86-87 1.5563394895206473E-4 88-89 5.706578128242374E-4 90-91 0.0034239468769454245 92-93 0.002230753268312928 94-95 0.22795185723179082 96-97 0.23552604274745798 98-99 0.01348827557584561 100-101 0.00788545341357128 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 963800.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 19.47479236749867 #Duplication Level Percentage of deduplicated Percentage of total 1 75.03387966832462 14.612692270685018 2 9.556173559979236 3.7220899181675238 3 3.9625959846978196 2.3151220211482193 4 2.2076540640148217 1.7197441806381306 5 1.416729208609909 1.3795253589324343 6 1.0142523402039445 1.1851412240232868 7 0.7611885416445758 1.037679216073306 8 0.6193446011821488 0.9649286009562895 9 0.500259136946243 0.876819853977495 >10 3.808537695195148 15.216233020442893 >50 0.5793451055030516 7.924681368753121 >100 0.46896801755869094 18.04454645263847 >500 0.03768973734685231 4.901231297478761 >1k 0.025844391323555874 10.508675268538443 >5k 0.005384248192407474 7.906645944869463 >10k+ 0.0021536992769629897 7.684244002677164 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 25860 2.683129279933596 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 21428 2.223282838763229 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 14459 1.500207511931936 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 11536 1.196928823407346 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 9598 0.9958497613612782 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 9504 0.9860967005602822 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9297 0.9646192156048972 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 9088 0.9429342187175763 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8286 0.8597219340112057 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6421 0.6662170574808052 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 6153 0.6384104586013696 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 5860 0.6080099605727329 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5762 0.5978418759078648 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 5435 0.5639136750363146 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 4948 0.5133845196098776 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4868 0.5050840423324341 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 4868 0.5050840423324341 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 4598 0.4770699315210625 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 4188 0.43452998547416477 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4145 0.43006847893753886 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 3861 0.40060178460261464 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 3364 0.3490350695164972 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3305 0.3429134675243827 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 3006 0.31189043369993774 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 2901 0.3009960572732932 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2837 0.29435567545133845 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 2798 0.29030919277858475 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2203 0.2285743930275991 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2164 0.22452791035484537 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2067 0.21446358165594523 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 1954 0.20273915750155633 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 1928 0.2000415023863872 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1805 0.1872795185723179 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1792 0.18593069101473333 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 1751 0.18167669641004358 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1750 0.18157294044407551 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1742 0.18074289271633118 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1681 0.17441377879228057 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1679 0.17420626686034446 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1660 0.17223490350695164 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1559 0.1617555509441793 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 1464 0.1518987341772152 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1410 0.14629591201494085 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1384 0.14359825689977174 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 1354 0.14048557792073044 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 1327 0.13768416683959328 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 1301 0.13498651172442414 No Hit TACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGA 1256 0.13031749325586223 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 1249 0.1295912014940859 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1151 0.11942311682921769 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 1133 0.1175555094417929 No Hit CTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACT 1128 0.1170367296119527 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 1122 0.11641419381614442 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 1114 0.11558414608840008 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 1110 0.11516912222452791 No Hit CTTTAATACCTTTTTAGGGTTTGCTGAAGATGGCGGTATATAGGCTGAAT 1097 0.11382029466694335 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1075 0.1115376634156464 No Hit GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA 1054 0.1093587881303175 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1030 0.10686864494708445 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1019 0.10572732932143597 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1014 0.10520854949159578 No Hit GCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAA 1005 0.10427474579788337 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 982 0.10188835858061839 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 976 0.10126582278481014 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 1.0375596596804317E-4 0.0 9 0.0 0.0 0.0 1.0375596596804317E-4 0.0 10-11 0.0 0.0 0.0 1.5563394895206476E-4 0.0 12-13 0.0 0.0 0.0 2.0751193193608634E-4 0.0 14-15 0.0 0.0 0.0 2.0751193193608634E-4 0.0 16-17 0.0 0.0 0.0 2.593899149201079E-4 0.0 18-19 0.0 0.0 0.0 4.150238638721727E-4 0.0 20-21 0.0 0.0 0.0 6.22535795808259E-4 0.0 22-23 0.0 0.0 0.0 0.0020232413363768416 0.0 24-25 0.0 0.0 0.0 0.004098360655737705 0.0 26-27 0.0 0.0 0.0 0.007677941481635194 0.0 28-29 0.0 0.0 0.0 0.018520439925295704 0.0 30-31 0.0 0.0 0.0 0.04881718198796431 0.0 32-33 0.0 0.0 0.0 0.10510479352562771 0.0 34-35 0.0 0.0 0.0 0.21285536418344053 0.0 36-37 0.0 0.0 0.0 0.3752334509234281 0.0 38-39 0.0 0.0 0.0 0.553226810541606 0.0 40-41 0.0 0.0 0.0 0.75980493878398 0.0 42-43 0.0 0.0 0.0 0.9629072421664245 0.0 44-45 0.0 0.0 0.0 1.1776302137372898 0.0 46-47 0.0 0.0 0.0 1.4622847063706164 0.0 48-49 0.0 0.0 0.0 1.7800373521477484 0.0 50-51 0.0 0.0 0.0 2.0802552396762817 0.0 52-53 0.0 0.0 0.0 2.3391782527495333 0.0 54-55 0.0 0.0 0.0 2.608528740402573 0.0 56-57 0.0 0.0 0.0 2.889344262295082 0.0 58-59 0.0 0.0 0.0 3.2214671093587883 0.0 60-61 0.0 0.0 0.0 3.641730649512347 0.0 62-63 0.0 0.0 0.0 3.9754617140485577 0.0 64-65 0.0 0.0 0.0 4.246472297157086 0.0 66-67 0.0 0.0 0.0 4.52656152728782 0.0 68-69 0.0 0.0 0.0 4.855104793525628 0.0 70-71 0.0 0.0 0.0 5.313187383274538 0.0 72-73 0.0 0.0 0.0 5.84701182818012 0.0 74-75 0.0 0.0 0.0 6.385816559452168 0.0 76-77 0.0 0.0 0.0 6.891730649512347 0.0 78-79 0.0 0.0 0.0 7.4785743930276 0.0 80-81 0.0 0.0 0.0 8.113560904752022 0.0 82-83 0.0 0.0 0.0 8.723697862627102 0.0 84-85 0.0 0.0 0.0 9.54554886905997 0.0 86-87 0.0 0.0 0.0 10.403299439717784 0.0 88-89 0.0 0.0 0.0 11.07636439095248 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGTCCG 15 0.009813727 48.11587 62-63 TTAGGCA 300 0.0 42.642685 4 TAGGCAT 300 0.0 42.642685 5 CCTTTAG 310 0.0 41.284245 1 CTTTAGG 315 0.0 40.62683 2 GCTACGC 25 0.0015173579 38.490673 68-69 GGATACG 65 1.3478086E-4 36.44674 8 TACTCGT 75 2.1827873E-11 35.349224 64-65 CATTCCG 370 0.0 34.57515 9 GCGGCGT 35 1.9047206E-4 34.366676 68-69 ATACCTT 515 0.0 34.040546 6 AGTACTC 185 0.0 33.29459 5 AGGCATT 415 0.0 33.109447 6 ATCGCCG 60 1.3243334E-7 32.13566 64-65 GACTTAT 60 0.003999546 31.587172 9 GGCATTC 435 0.0 31.587172 7 AATACCT 560 0.0 31.305145 5 TTGAATC 95 3.5506582E-5 29.924692 4 GGATATG 485 0.0 29.319855 1 TAATACC 605 0.0 28.976664 4 >>END_MODULE