##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939434_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 959463 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.856095545112215 31.0 31.0 31.0 28.0 34.0 2 31.061574026304296 31.0 31.0 34.0 28.0 34.0 3 31.059024683599056 31.0 31.0 34.0 27.0 34.0 4 34.91241767530379 35.0 35.0 37.0 32.0 37.0 5 34.54645358914309 35.0 35.0 37.0 30.0 37.0 6 34.64403942622071 35.0 35.0 37.0 31.0 37.0 7 34.5127701641439 35.0 35.0 37.0 30.0 37.0 8 34.45736208691737 35.0 35.0 37.0 30.0 37.0 9 35.85169308248468 37.0 35.0 39.0 30.0 39.0 10-11 35.66944426205075 37.0 35.0 39.0 30.0 39.0 12-13 35.67635281402201 37.0 35.0 39.0 30.0 39.0 14-15 36.21327555101135 38.0 34.0 40.0 30.0 41.0 16-17 36.181628160752425 38.0 34.0 40.0 29.5 41.0 18-19 36.423116889343305 38.0 35.0 40.0 30.0 41.0 20-21 36.34995408890181 38.0 34.5 40.0 29.5 41.0 22-23 36.192982949837564 38.0 34.0 40.0 29.5 41.0 24-25 36.00269890553362 38.0 34.0 40.0 29.0 41.0 26-27 35.30895250780905 37.5 33.5 40.0 26.5 41.0 28-29 35.104354206467576 37.0 33.0 40.0 26.5 41.0 30-31 34.8987230356981 37.0 33.0 39.0 27.0 40.5 32-33 34.70102755395466 37.0 33.0 40.0 26.0 41.0 34-35 34.93054656615211 37.0 33.0 40.0 27.0 41.0 36-37 34.61592005111193 37.0 33.0 40.0 25.0 41.0 38-39 34.246798469560574 37.0 33.0 40.0 24.5 41.0 40-41 34.072458239661145 37.0 33.0 40.0 24.0 41.0 42-43 34.01702202169338 37.0 33.0 40.0 24.0 41.0 44-45 33.62643322358444 37.0 33.0 40.0 23.0 41.0 46-47 33.05553262606271 37.0 31.5 40.0 19.5 41.0 48-49 32.60036551696105 37.0 31.0 40.0 15.5 41.0 50-51 32.36286964687539 37.0 31.0 39.0 14.0 41.0 52-53 32.03562774176805 36.0 30.5 39.0 13.0 40.0 54-55 31.789976789099736 36.0 30.0 39.0 8.5 40.0 56-57 31.48246258584229 36.0 29.5 39.0 8.0 40.0 58-59 31.121209989337785 35.5 29.0 39.0 7.5 40.0 60-61 30.70722060152398 35.0 28.0 39.0 4.0 40.0 62-63 30.45380384652665 35.0 28.0 38.0 2.0 40.0 64-65 29.802804276975767 34.0 28.0 38.0 2.0 40.0 66-67 28.939973193338357 33.5 26.5 37.5 2.0 39.5 68-69 25.691942784661837 31.0 15.5 36.0 2.0 39.0 70-71 24.37688321488166 29.5 10.5 35.0 2.0 38.0 72-73 25.251706423280524 30.0 16.5 35.0 2.0 37.5 74-75 25.921911006469244 31.0 21.0 35.0 2.0 37.0 76-77 25.321170800750004 30.5 20.5 34.0 2.0 36.0 78-79 26.33216080244887 31.5 23.5 35.0 2.0 36.0 80-81 26.412971109881255 32.0 24.0 35.0 2.0 36.0 82-83 26.137984997858176 32.0 23.5 35.0 2.0 36.0 84-85 25.79836272998542 32.0 22.5 34.5 2.0 35.5 86-87 25.288113246680695 31.0 20.0 34.0 2.0 35.0 88-89 24.614452042444576 31.0 19.0 34.0 2.0 35.0 90-91 23.922522285903675 30.5 13.0 34.0 2.0 35.0 92-93 23.529112639049135 30.0 7.0 34.0 2.0 35.0 94-95 22.812931817068506 30.0 2.0 34.0 2.0 35.0 96-97 22.037673156755393 29.0 2.0 34.0 2.0 35.0 98-99 21.179827674438723 29.0 2.0 34.0 2.0 35.0 100-101 19.79325883332656 27.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.23747944015670086 1101 2 0.11815976461987532 1101 3 0.16834436960681032 1101 4 0.3458875170844564 1101 5 0.24842051430276513 1101 6 0.4170908841304879 1101 7 0.41701521283118126 1101 8 0.5000545962314504 1101 9 0.3773273965371189 1101 10-11 0.5251135932501754 1101 12-13 0.3737623894401949 1101 14-15 0.24747042178113787 1101 16-17 0.3764820553155914 1101 18-19 0.22652814663243248 1101 20-21 0.5153342114047277 1101 22-23 0.46459815924581704 1101 24-25 0.5068146562693343 1101 26-27 0.5509681253541956 1101 28-29 0.6168105470591669 1101 30-31 0.32895785216010864 1101 32-33 0.5152366358552385 1101 34-35 0.5542911000690296 1101 36-37 0.6624784650851154 1101 38-39 0.9074908023334629 1101 40-41 0.9530298264339905 1101 42-43 0.44999081575702604 1101 44-45 0.6295954574044487 1101 46-47 0.5341349213164364 1101 48-49 0.5151627245498744 1101 50-51 0.379745239705926 1101 52-53 -0.18971384756608956 1101 54-55 -0.03985242686015411 1101 56-57 0.03273363109933669 1101 58-59 0.05217856767215778 1101 60-61 -0.035789490091570286 1101 62-63 -0.3317614859428666 1101 64-65 -0.3408702998550659 1101 66-67 -0.4931231703429937 1101 68-69 -7.366711892628313 1101 70-71 -7.648858464872838 1101 72-73 -4.631147336801789 1101 74-75 -2.525194405268117 1101 76-77 -0.2919894672198886 1101 78-79 -0.08851222450641316 1101 80-81 -0.019705931970911195 1101 82-83 -0.1465618520636518 1101 84-85 -0.18259442898033384 1101 86-87 -0.40973081154004376 1101 88-89 -0.10908574227894619 1101 90-91 -0.4256207795469322 1101 92-93 -0.44801161062145667 1101 94-95 -0.2475098329763945 1101 96-97 -0.5735925462402456 1101 98-99 -0.8118445326990091 1101 100-101 -0.4046689338195293 1102 1 0.17770668874966944 1102 2 0.21841674667360778 1102 3 0.3006115459899448 1102 4 0.3308508812131592 1102 5 0.1925810749908763 1102 6 0.36676063033416284 1102 7 0.27141470311471494 1102 8 0.5633826200182241 1102 9 0.688815131484553 1102 10-11 0.4326616446465792 1102 12-13 0.7027057896738143 1102 14-15 0.6504480154946393 1102 16-17 0.46371153391811504 1102 18-19 0.30301959826246616 1102 20-21 0.21185964413276537 1102 22-23 0.292959633175947 1102 24-25 0.3487940552286659 1102 26-27 0.40695623196989317 1102 28-29 0.5823579613099881 1102 30-31 0.5929548788140266 1102 32-33 0.6824724859136424 1102 34-35 0.6050397461346577 1102 36-37 0.7076520872577774 1102 38-39 0.7211766895586962 1102 40-41 0.6630712408971959 1102 42-43 0.4062741331617232 1102 44-45 0.2334183309310518 1102 46-47 0.6967217324027644 1102 48-49 0.7515883378025023 1102 50-51 0.5350983245524787 1102 52-53 0.6064271739906673 1102 54-55 0.0977816391906039 1102 56-57 0.2654000853841403 1102 58-59 0.1921467103927661 1102 60-61 0.16939262735559524 1102 62-63 -0.02003375949070474 1102 64-65 -0.2256701299495738 1102 66-67 -0.17754708454071633 1102 68-69 -7.065809269712311 1102 70-71 -7.337493911405705 1102 72-73 -1.415121001450828 1102 74-75 0.20700056128173827 1102 76-77 0.6583365389391851 1102 78-79 0.37124141253298504 1102 80-81 0.1806338790092319 1102 82-83 0.3691225484885372 1102 84-85 0.2776264481567594 1102 86-87 0.043339168283676344 1102 88-89 -0.20016570405021028 1102 90-91 -0.356460749813472 1102 92-93 -0.2398667661911702 1102 94-95 0.12864605531074602 1102 96-97 0.10045863778864117 1102 98-99 -0.27861101884109374 1102 100-101 0.028093092303439704 1103 1 0.2869827834427916 1103 2 0.24384797690802884 1103 3 0.28999035976619325 1103 4 0.22751416244381062 1103 5 0.4125493674925522 1103 6 0.34731419040914346 1103 7 0.28046868017631965 1103 8 0.3984573886112841 1103 9 0.5405033080556834 1103 10-11 0.46070213776423685 1103 12-13 0.4985582176920147 1103 14-15 0.7104032616818969 1103 16-17 0.5412652673934701 1103 18-19 0.6560264434489582 1103 20-21 0.6428231722525268 1103 22-23 0.5658517863958252 1103 24-25 0.648767219455344 1103 26-27 0.6736406515327573 1103 28-29 0.625649017978894 1103 30-31 0.6704532893105295 1103 32-33 0.8251516869507398 1103 34-35 0.6697143727045543 1103 36-37 0.7981212591471021 1103 38-39 1.0222542490721835 1103 40-41 0.6940269247139881 1103 42-43 0.6916742338990858 1103 44-45 0.954268488229971 1103 46-47 0.8817845281123411 1103 48-49 0.8750617951583948 1103 50-51 0.7965382082916506 1103 52-53 0.3971535564458506 1103 54-55 0.5248622373393523 1103 56-57 0.655173178599231 1103 58-59 1.0569352857847747 1103 60-61 0.9080580224194676 1103 62-63 0.49251960241240766 1103 64-65 0.43017263719022836 1103 66-67 0.2194324459571071 1103 68-69 -6.8521486225160295 1103 70-71 -7.494415889491197 1103 72-73 -0.8952063804958037 1103 74-75 0.43870062816474586 1103 76-77 0.7134497859026112 1103 78-79 0.6222851176862783 1103 80-81 0.4587540537307575 1103 82-83 0.28652616677650045 1103 84-85 7.725611996534099E-5 1103 86-87 -0.013462255250882293 1103 88-89 0.24592287877324637 1103 90-91 -0.053937361404873485 1103 92-93 0.014962021575023243 1103 94-95 0.3944481972183169 1103 96-97 0.2328345533013696 1103 98-99 0.5463883618718306 1103 100-101 0.658129754098379 1104 1 0.2727644378767202 1104 2 0.27541135024355157 1104 3 0.1934838630363167 1104 4 0.29286343218623045 1104 5 0.3904981994994614 1104 6 0.38922784885295414 1104 7 0.34217874970470774 1104 8 0.3445366258297611 1104 9 0.44340209703042177 1104 10-11 0.3995102271680153 1104 12-13 0.3566310210365984 1104 14-15 0.6653065118196935 1104 16-17 0.4944528715490577 1104 18-19 0.48520161179017407 1104 20-21 0.5259037923026213 1104 22-23 0.434679409504902 1104 24-25 0.4504630416277067 1104 26-27 0.6731797076615393 1104 28-29 0.3994263079512663 1104 30-31 0.41046885825387136 1104 32-33 0.27821760837109366 1104 34-35 0.1158008625363891 1104 36-37 0.4768216230671243 1104 38-39 0.5346006145458801 1104 40-41 0.4395174108969542 1104 42-43 0.28154700694386037 1104 44-45 0.6782025958200677 1104 46-47 0.2526968746389855 1104 48-49 0.34319945298332044 1104 50-51 0.47320542293354606 1104 52-53 0.3719571717930279 1104 54-55 0.2188301582246197 1104 56-57 0.4272380459731089 1104 58-59 0.5321901748520261 1104 60-61 0.6215499784654064 1104 62-63 0.19357574408656575 1104 64-65 0.11696288838851743 1104 66-67 0.1589545148537077 1104 68-69 -6.397373501880896 1104 70-71 -3.5439842784117843 1104 72-73 0.33675798177384664 1104 74-75 0.9485762700210287 1104 76-77 1.2393843254261334 1104 78-79 0.7529191894968186 1104 80-81 0.4620894500296693 1104 82-83 0.33403964857888724 1104 84-85 0.48317060853573324 1104 86-87 0.43961172462875453 1104 88-89 0.1809797553788961 1104 90-91 -0.03269203934048903 1104 92-93 -0.10061078982801774 1104 94-95 0.4790660489620038 1104 96-97 0.3692570164178015 1104 98-99 0.464998728918534 1104 100-101 0.9141856862525977 1105 1 0.12332998054948519 1105 2 0.21853152121182617 1105 3 0.29270283716221357 1105 4 0.25283061814000973 1105 5 0.32846256821587616 1105 6 0.0941496334471168 1105 7 0.27323540712026073 1105 8 0.40478650253533743 1105 9 0.5522573767717773 1105 10-11 0.4462355916521261 1105 12-13 0.4366233171495182 1105 14-15 0.5919584153889517 1105 16-17 0.5154967321312682 1105 18-19 0.5913790655104378 1105 20-21 0.6627146731566924 1105 22-23 0.7444232149672487 1105 24-25 0.5113350313902387 1105 26-27 0.6560095484586199 1105 28-29 0.5302602295521197 1105 30-31 0.400561788406371 1105 32-33 0.6917882149796739 1105 34-35 0.6340000869902696 1105 36-37 0.5711773170132872 1105 38-39 0.6149305601029269 1105 40-41 0.6420377746235744 1105 42-43 0.5913125702462878 1105 44-45 0.7969447992607144 1105 46-47 0.7497772948392907 1105 48-49 0.8361829524820834 1105 50-51 0.6776412824327025 1105 52-53 0.46360925264838926 1105 54-55 0.49638122468111945 1105 56-57 0.6827500321254512 1105 58-59 0.6717634955496976 1105 60-61 0.8596855088570798 1105 62-63 0.35825197130932906 1105 64-65 0.5242051869189801 1105 66-67 0.6534288293205783 1105 68-69 -2.1224922029861943 1105 70-71 -2.455537046814886 1105 72-73 1.1034373808061844 1105 74-75 1.4572358903708924 1105 76-77 1.0705926430454724 1105 78-79 0.6471494938164746 1105 80-81 0.3886817210001965 1105 82-83 0.3240487707547999 1105 84-85 0.2844353031362381 1105 86-87 0.35498259104206653 1105 88-89 0.26852685707342516 1105 90-91 0.36604455541248626 1105 92-93 0.43494393839237944 1105 94-95 0.5874861719018618 1105 96-97 0.6207188209324705 1105 98-99 0.6996433347470585 1105 100-101 0.5681659204636595 1106 1 0.19264773315416406 1106 2 0.16610199047208596 1106 3 0.1291035150308737 1106 4 0.10414741477607237 1106 5 0.31376204377015426 1106 6 0.1712137401679783 1106 7 0.2062006145420554 1106 8 0.3704986490191402 1106 9 0.29597589415135417 1106 10-11 0.4465949155215938 1106 12-13 0.19852445796102103 1106 14-15 0.23064155190193247 1106 16-17 0.20627013080026302 1106 18-19 0.18421331191636625 1106 20-21 0.3073567364444898 1106 22-23 0.3170191711531416 1106 24-25 0.4174788010486026 1106 26-27 0.6018067962049045 1106 28-29 0.36592888949135727 1106 30-31 0.23589117976187168 1106 32-33 0.30065081287454376 1106 34-35 -0.09092662765309711 1106 36-37 0.021316534121787356 1106 38-39 0.228327323848319 1106 40-41 0.23200244389519753 1106 42-43 0.23348950466215967 1106 44-45 0.459692607385108 1106 46-47 0.3267979484410546 1106 48-49 0.5177367691700852 1106 50-51 0.3091713670325831 1106 52-53 0.07428434699179576 1106 54-55 -0.1282782532797917 1106 56-57 -0.03326043673370194 1106 58-59 0.015711645950968745 1106 60-61 0.3174323643554011 1106 62-63 -0.2908699414301239 1106 64-65 -0.2656388163834613 1106 66-67 -0.03284633588483743 1106 68-69 -2.990444859141661 1106 70-71 -3.3958266117154636 1106 72-73 0.2097880541624022 1106 74-75 0.6264356510444813 1106 76-77 0.7061504975310058 1106 78-79 0.2060585531232988 1106 80-81 -0.022173369532705323 1106 82-83 -0.030216152884698033 1106 84-85 -0.1917398530443002 1106 86-87 -0.19786475815054416 1106 88-89 -0.196037124301899 1106 90-91 0.059042419524555356 1106 92-93 -0.3316100287102657 1106 94-95 0.19050933909943524 1106 96-97 0.1715138458845935 1106 98-99 0.11268110887774441 1106 100-101 0.005687855932436037 1107 1 -0.03565451511714812 1107 2 0.08870745912507516 1107 3 0.004118149904090984 1107 4 -0.19118787460415376 1107 5 0.05455094326316612 1107 6 0.012277243100271562 1107 7 -0.10816355786916887 1107 8 -0.08235222428365319 1107 9 0.170820232318043 1107 10-11 -0.025258231951802657 1107 12-13 0.04459397449576841 1107 14-15 0.13880461809413447 1107 16-17 0.0615090606273796 1107 18-19 0.29629692759927195 1107 20-21 0.009650597603005906 1107 22-23 0.15049826191064142 1107 24-25 0.008161797277246308 1107 26-27 0.46545323895505675 1107 28-29 0.21037792642800213 1107 30-31 0.15539582921675077 1107 32-33 0.19188886993103438 1107 34-35 0.12841286947492137 1107 36-37 0.3782486744320792 1107 38-39 0.3390388092968166 1107 40-41 0.2206475562023229 1107 42-43 -0.15702922620163662 1107 44-45 0.48928166014515284 1107 46-47 0.5486227131300296 1107 48-49 0.6264995990756077 1107 50-51 0.296651011254383 1107 52-53 0.18369998093901785 1107 54-55 0.29834781417899237 1107 56-57 0.30773831797163 1107 58-59 0.4394267040824573 1107 60-61 0.12048422325038999 1107 62-63 -0.02796020932812837 1107 64-65 -0.1473846059747821 1107 66-67 -0.032558960192563546 1107 68-69 -2.1971877217583433 1107 70-71 -2.2967547287660643 1107 72-73 1.0116496100356862 1107 74-75 1.1715970958701263 1107 76-77 0.5945493891832712 1107 78-79 0.5327594801759936 1107 80-81 0.11329411266596878 1107 82-83 -0.010729597454893991 1107 84-85 -0.004839217624709846 1107 86-87 -0.03975402271711914 1107 88-89 0.2847566002644655 1107 90-91 0.42749353550016167 1107 92-93 0.4498859923781424 1107 94-95 0.5698177570785106 1107 96-97 0.6035024856751257 1107 98-99 0.7625847848031135 1107 100-101 0.8734090021711829 1108 1 0.08061897504042292 1108 2 0.12583418322452644 1108 3 0.17121258666107408 1108 4 -0.011089227101585664 1108 5 0.12880811120198388 1108 6 -0.11271531741989804 1108 7 0.2314338174823476 1108 8 0.3546669993322311 1108 9 0.39213359808750425 1108 10-11 0.45522987231993994 1108 12-13 0.37682440494406677 1108 14-15 0.36194716596718735 1108 16-17 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0.020827445954118673 2316 96-97 -0.3773740529941776 2316 98-99 -0.09500425975652149 2316 100-101 -0.009450997598950295 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 2.0 4 23.0 5 66.0 6 236.0 7 569.0 8 1070.0 9 1858.0 10 3191.0 11 4807.0 12 6428.0 13 8171.0 14 10165.0 15 12814.0 16 15378.0 17 17287.0 18 15055.0 19 12599.0 20 12132.0 21 12216.0 22 12972.0 23 13844.0 24 16097.0 25 19422.0 26 23606.0 27 29444.0 28 35776.0 29 42411.0 30 49079.0 31 57208.0 32 65505.0 33 75467.0 34 85405.0 35 94149.0 36 93593.0 37 76403.0 38 32946.0 39 2065.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.709041591697094 21.487827303451137 15.396162530569866 18.40696857428191 2 13.67376404055146 24.780276771520157 39.24974590185296 22.29621328607542 3 21.529379516207538 30.224365555244038 32.82332476254897 15.422930165999452 4 10.871340984417202 18.468280968163604 41.45835876218107 29.202019285238123 5 9.666761511387099 43.35946253268755 35.1718617601721 11.801914195753248 6 23.22903540834821 40.887246303400964 18.85940364558091 17.02431464266991 7 22.64641783997924 39.22412849687794 21.25762014793692 16.871833515205903 8 22.508736657901345 32.68151038653914 21.516619192193968 23.29313376336555 9 24.618562675163087 20.92587207635938 23.71274348255222 30.742821765925317 10-11 21.686560497902562 27.253879961579536 28.45735990106157 22.602199639456337 12-13 28.713040523709616 26.271414322386583 27.019436914190543 17.99610823971326 14-15 25.120770681099742 26.989003223678242 30.447083420621745 17.44314267460027 16-17 17.207849227297718 30.955044908190306 32.50076214944382 19.33634371506815 18-19 16.42363115996256 32.822196189114315 34.008850793465506 16.74532185745762 20-21 18.552409003786494 30.147697201455397 30.75960198569408 20.54029180906403 22-23 17.938267358722616 29.134252600421068 29.82333812769683 23.104141913159484 24-25 19.387418486885203 29.70649806682395 32.14836077406255 18.757722672228294 26-27 21.47417189552021 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17.83520208604954 52-53 17.92772574493979 30.638083309782115 32.925645242532354 18.508545702745742 54-55 16.094965484160255 29.503170034828674 33.66102525600117 20.740839225009907 56-57 19.233826680796565 32.39819202654724 28.656931772511445 19.711049520144748 58-59 18.16623803899452 29.716355160159413 30.48449695234779 21.632909848498276 60-61 18.38931351420374 28.459335430255617 31.662935812809295 21.48841524273135 62-63 22.426259067789545 27.80611606770616 30.302363878929377 19.46526098557492 64-65 20.87819882415542 28.60129112550559 32.25817715426016 18.262332896078835 66-67 17.833134656776682 29.316413467139036 32.326676450878175 20.523775425206107 68-69 17.91422951810992 31.747907284858595 30.75686290360256 19.581000293428925 70-71 18.24578709585146 31.107080960113077 29.29708861611462 21.350043327920847 72-73 18.388815407892746 29.20751922404068 28.468368392397934 23.93529697566864 74-75 18.97645501362686 30.762973935595284 29.539606539016834 20.72096451176102 76-77 19.770082894217456 28.39079955639376 31.10637360250458 20.73274394688421 78-79 18.283485045513654 28.411361083675807 32.36631911417113 20.938834756639416 80-81 17.811330351680123 32.20221945898733 30.238851893273356 19.747598296059195 82-83 17.4862616245287 31.68203575495272 28.947753785381693 21.883948835136888 84-85 18.032468229558056 28.88934516358664 28.85807760815708 24.22010899869823 86-87 19.537034190096456 29.425308081916153 29.811729599784044 21.22592812820335 88-89 21.256718629977936 26.829859326974887 30.750975289199435 21.162446753847743 90-91 18.192767065832687 28.002213844449287 32.59573261678707 21.20928647293096 92-93 17.55577756619018 31.04787456086453 30.685205075809336 20.71114279713596 94-95 17.412394339166063 31.17098906050517 28.948252267587886 22.46836433274088 96-97 17.403675669814266 29.44675189671882 27.5461491903006 25.60342324316631 98-99 18.631605775540315 28.988248465191287 28.09428330335555 24.285862455912852 100-101 19.91081373089117 29.325093645061372 28.961406299431747 21.802686324615713 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3275.0 1 2125.0 2 1016.0 3 1105.0 4 1003.5 5 996.5 6 1643.0 7 2783.5 8 3621.0 9 3782.5 10 3419.0 11 2883.5 12 2577.5 13 2430.0 14 2338.0 15 2320.0 16 2326.0 17 2459.5 18 2713.5 19 2881.0 20 3182.5 21 3675.0 22 4150.5 23 4588.5 24 5126.5 25 6330.0 26 8500.0 27 10695.5 28 11797.0 29 12775.5 30 14538.5 31 16610.0 32 20449.0 33 26052.5 34 31553.0 35 35153.5 36 39398.5 37 43625.0 38 40950.5 39 34951.5 40 30610.5 41 27161.0 42 24387.0 43 23755.5 44 25313.0 45 26650.0 46 32112.0 47 50833.5 48 98818.0 49 96755.0 50 46820.0 51 24461.5 52 11156.5 53 5070.5 54 2970.5 55 2217.0 56 1861.5 57 1532.0 58 1180.0 59 924.5 60 740.5 61 569.0 62 439.0 63 348.0 64 299.0 65 264.0 66 211.5 67 202.5 68 203.5 69 191.5 70 186.0 71 176.5 72 166.5 73 146.0 74 130.5 75 121.0 76 89.5 77 66.0 78 51.5 79 34.5 80 26.5 81 15.0 82 12.0 83 9.5 84 3.0 85 1.5 86 0.5 87 0.5 88 1.5 89 2.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01928161898895528 2 0.01959429389147888 3 2.0844993501573276E-4 4 0.09442782056212694 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.00818165994936751 12-13 0.0 14-15 0.0 16-17 5.732373212932651E-4 18-19 1.0422496750786638E-4 20-21 0.0 22-23 3.1267490252359913E-4 24-25 0.003283086476497791 26-27 0.004481673602838254 28-29 0.0015633745126179955 30-31 1.0422496750786638E-4 32-33 5.732373212932651E-4 34-35 0.0028661866064663257 36-37 0.0876010851903617 38-39 0.09474049546465053 40-41 0.07921097530597845 42-43 0.0832236365550313 44-45 0.08145181210739758 46-47 0.08051378739982677 48-49 0.07514620157317166 50-51 0.07431240183310872 52-53 0.05836598180440517 54-55 0.05034065930629946 56-57 0.027880178808354257 58-59 0.020844993501573275 60-61 0.012559108584697897 62-63 0.0023971742526809267 64-65 0.007035185306780981 66-67 0.15185577765896133 68-69 0.7225395872482837 70-71 0.8937290963799542 72-73 0.3578564259382592 74-75 0.05331107088027365 76-77 0.19354576466210788 78-79 0.014122483097315895 80-81 0.004168998700314655 82-83 0.001615486996371929 84-85 2.0844993501573276E-4 86-87 0.001928161898895528 88-89 4.168998700314655E-4 90-91 0.004794348505361853 92-93 0.005159135891639386 94-95 0.07660535111828179 96-97 0.03882380039668023 98-99 0.014747832902363091 100-101 0.012767558519713633 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 959463.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 19.980765655812228 #Duplication Level Percentage of deduplicated Percentage of total 1 75.15711852317033 15.016967725775707 2 9.638046857823765 3.85151111291828 3 3.8841756258980937 2.3282640884126278 4 2.1571669969500684 1.7240739298604608 5 1.4456975408659294 1.444307188661308 6 1.0255402273370855 1.229464737181842 7 0.7836201381989274 1.0960131241169573 8 0.5876252792018626 0.9392962397730915 9 0.49752993302337845 0.8946926098642869 >10 3.7235124715348733 15.272838912956194 >50 0.572366240329187 8.003078367616535 >100 0.45725347691101764 17.80742970240332 >500 0.03943678005992459 5.255168093863666 >1k 0.022915966791577803 9.401844133562946 >5k 0.005862224062961764 8.271203534992587 >10k+ 0.002131717841077005 7.463846498040197 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 24693 2.5736271226717444 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 20414 2.127648486705584 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 13605 1.417980682944522 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 11382 1.186288580174535 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 9500 0.9901371913247307 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9124 0.9509486035417729 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 9032 0.9413599065310492 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 8808 0.9180135138092871 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7750 0.8077434981859644 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5963 0.6214934812494072 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 5855 0.6102371847585577 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5767 0.6010653876178654 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 5628 0.586578117134272 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 5205 0.5424909558784445 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 5044 0.525710736109678 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 4889 0.5095558661459587 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4814 0.5017389935828688 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 4460 0.464843355085084 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 4023 0.4192970442841465 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3980 0.4148153706813082 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 3575 0.3726042588406223 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 3342 0.34831984141128947 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2984 0.3110073030434733 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 2800 0.2918299090220258 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 2752 0.2868271105816483 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2731 0.2846383862639831 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 2615 0.2725482900330706 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2183 0.2275231040696723 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2149 0.22397945517440487 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1959 0.20417671134791024 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 1906 0.1986527880699933 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 1886 0.19656828871983598 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1790 0.18656269183908084 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1728 0.1801007438535931 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1709 0.17812046947094365 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1669 0.17395147077062897 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1653 0.17228387129050313 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1630 0.1698866970378222 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 1600 0.1667599480125862 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1560 0.16259094931227155 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1516 0.15800505074192545 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 1458 0.1519600026264692 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1379 0.14372623019334774 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1320 0.13757695711038362 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 1317 0.13726428220786002 No Hit TACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGA 1270 0.1323657087349903 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 1266 0.13194880886495883 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 1246 0.12986430951480152 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 1215 0.12663333552205766 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 1170 0.12194321198420366 No Hit CTTTAATACCTTTTTAGGGTTTGCTGAAGATGGCGGTATATAGGCTGAAT 1145 0.119337587796507 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1139 0.11871223799145982 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 1125 0.11725308844634967 No Hit CTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACT 1119 0.11662773864130246 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 1100 0.11464746425865302 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 1049 0.10933199091575183 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1041 0.1084981911756889 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1032 0.1075601664681181 No Hit GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA 994 0.10359961770281918 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 986 0.10276581796275625 No Hit TCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTA 974 0.10151511835266186 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 960 0.10005596880755172 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.0422496750786638E-4 0.0 10-11 0.0 0.0 0.0 1.0422496750786638E-4 0.0 12-13 0.0 0.0 0.0 1.0422496750786638E-4 0.0 14-15 0.0 0.0 0.0 1.0422496750786638E-4 0.0 16-17 0.0 0.0 0.0 1.0422496750786638E-4 0.0 18-19 0.0 0.0 0.0 1.0422496750786638E-4 0.0 20-21 0.0 0.0 0.0 2.6056241876966596E-4 0.0 22-23 0.0 0.0 0.0 0.0013549245776022629 0.0 24-25 0.0 0.0 0.0 0.002501399220188793 0.0 26-27 0.0 0.0 0.0 0.005680260729178718 0.0 28-29 0.0 0.0 0.0 0.01651965734999682 0.0 30-31 0.0 0.0 0.0 0.045077298447152206 0.0 32-33 0.0 0.0 0.0 0.10318271783278771 0.0 34-35 0.0 0.0 0.0 0.21397385829364968 0.0 36-37 0.0 0.0 0.0 0.37760705728099986 0.0 38-39 0.0 0.0 0.0 0.5654725612139291 0.0 40-41 0.0 0.0 0.0 0.7730365840058449 0.0 42-43 0.0 0.0 0.0 0.9787245573826192 0.0 44-45 0.0 0.0 0.0 1.1904575788748497 0.0 46-47 0.0 0.0 0.0 1.468008667348298 0.0 48-49 0.0 0.0 0.0 1.7793807577780487 0.0 50-51 0.0 0.0 0.0 2.0791838768144264 0.0 52-53 0.0 0.0 0.0 2.3329195602123267 0.0 54-55 0.0 0.0 0.0 2.583789057003761 0.0 56-57 0.0 0.0 0.0 2.863424644827367 0.0 58-59 0.0 0.0 0.0 3.1988727027514345 0.0 60-61 0.0 0.0 0.0 3.6399006527609714 0.0 62-63 0.0 0.0 0.0 3.975296598201285 0.0 64-65 0.0 0.0 0.0 4.258788509822683 0.0 66-67 0.0 0.0 0.0 4.565314139263317 0.0 68-69 0.0 0.0 0.0 4.902534021635018 0.0 70-71 0.0 0.0 0.0 5.358622479449442 0.0 72-73 0.0 0.0 0.0 5.884437440526629 0.0 74-75 0.0 0.0 0.0 6.4219255979646945 0.0 76-77 0.0 0.0 0.0 6.9126688574754835 0.0 78-79 0.0 0.0 0.0 7.493879388783101 0.0 80-81 0.0 0.0 0.0 8.124440442205692 0.0 82-83 0.0 0.0 0.0 8.73681423879816 0.0 84-85 0.0 1.0422496750786638E-4 0.0 9.56581963035573 0.0 86-87 0.0 1.0422496750786638E-4 0.0 10.448917780049882 0.0 88-89 0.0 1.0422496750786638E-4 0.0 11.151602510987917 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGGCGT 15 0.009874311 48.040962 68-69 ATCGAGT 15 0.009886679 48.02574 64-65 TAGGCAT 380 0.0 46.15502 5 TTAGGCA 395 0.0 44.443996 4 CCTTTAG 395 0.0 43.249577 1 CATTCCG 430 0.0 40.78816 9 GTACGCG 25 0.0015310046 38.420593 64-65 AGGCATT 480 0.0 37.526943 6 CTTTAGG 470 0.0 36.342358 2 GGCATTC 485 0.0 36.162697 7 GCATTCC 515 0.0 34.056133 8 GACATAT 285 0.0 33.30133 1 TATGTAC 160 5.4569682E-11 32.619793 4 GGTATCG 170 0.0 32.41867 62-63 GTATCGA 170 0.0 32.41867 62-63 ATGGGGA 855 0.0 32.15605 5 TCGATGC 30 0.003740896 32.02308 66-67 GTCTTGA 75 3.0954595E-4 31.636263 1 TGTCTCG 85 9.822543E-11 31.009163 62-63 AGGCTTA 525 0.0 30.69873 6 >>END_MODULE