FastQCFastQC Report
Fri 27 May 2016
SRR939433_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939433_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2316816
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36510.1575869641784242No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30820.13302739621963935No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39850.040.0656741
GTATCAA66100.038.1944121
ATCAACG83100.030.5435643
TATCAAC84300.030.5298652
TCAACGC84200.029.9521884
CAACGCA85600.028.9235295
AACGCAG86950.028.155746
GTACATG68750.024.9652841
ACGCAGA98650.024.38617
CGCAGAG99400.024.0178
TACATGG70050.023.7977852
ACATGGG72000.022.6147233
GCAGAGT104150.021.6326969
GAGTACT56300.020.7508412-13
AGAGTAC85800.020.20844710-11
CATGGGG42200.020.1854114
GTACTTT60300.019.29063614-15
GTATAGG8150.018.9781951
CAGAGTA101000.018.37954710-11
AGTACTT62350.018.12298612-13
Produced by FastQC (version 0.11.5)