FastQCFastQC Report
Fri 27 May 2016
SRR939433_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939433_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2316816
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50280.21702198189239025No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50030.21594291475887598No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29250.12625085462116975No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43250.044.5929531
GTATCAA67100.034.3925481
ATCAACG84700.026.7321363
TCAACGC86150.026.2822044
TATCAAC87700.026.0916962
CAACGCA87850.025.7736155
AACGCAG91550.024.8354516
GTACATG74700.022.6467321
TACATGG75350.021.9430482
ACGCAGA105350.021.4023577
CGCAGAG107000.020.9837828
ACATGGG75800.020.8096223
GAGTACT61900.019.97452712-13
GTACTTT64150.019.2739414-15
GCAGAGT115350.018.5613699
AGAGTAC98650.018.34276610-11
CGTTATA1600.001212649317.7658542
GTGGTAT25750.017.6665551
CAGAGTA109850.017.65844310-11
TATCGTT2202.989589E-517.2248927
Produced by FastQC (version 0.11.5)