##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939433_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2316816 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.95721541978301 31.0 31.0 31.0 30.0 34.0 2 31.227807905332146 31.0 31.0 34.0 30.0 34.0 3 31.22722693558746 31.0 31.0 34.0 28.0 34.0 4 35.16499497586343 35.0 35.0 37.0 33.0 37.0 5 34.77226503960608 35.0 35.0 37.0 32.0 37.0 6 34.72326157968522 35.0 35.0 37.0 32.0 37.0 7 34.58683555362187 35.0 35.0 37.0 30.0 37.0 8 34.59430615120061 35.0 35.0 37.0 30.0 37.0 9 35.966138010096614 37.0 35.0 39.0 30.0 39.0 10-11 35.82139475038156 37.0 35.0 39.0 30.0 39.0 12-13 35.784704525521235 37.0 35.0 39.0 30.0 39.0 14-15 36.685065408733365 38.0 36.0 40.0 31.0 41.0 16-17 36.68045261255102 38.0 36.0 40.0 31.0 41.0 18-19 36.589255901202336 38.0 35.0 40.0 30.0 41.0 20-21 36.467969834462465 38.0 35.0 40.0 30.0 41.0 22-23 36.32929740644056 38.0 34.0 40.0 30.0 41.0 24-25 36.12488561888385 38.0 34.0 40.0 29.0 41.0 26-27 35.75778568518173 38.0 34.0 40.0 27.5 41.0 28-29 35.480131568497455 38.0 34.0 40.0 27.0 41.0 30-31 35.28540678241173 38.0 34.0 40.0 27.0 41.0 32-33 35.1256118310647 38.0 33.0 40.0 27.0 41.0 34-35 35.137879745305625 38.0 33.5 40.0 26.5 41.0 36-37 35.193386527026746 38.0 33.5 40.0 26.5 41.0 38-39 35.18470780588532 38.0 33.5 40.0 27.0 41.0 40-41 34.99511614215372 38.0 33.0 40.0 26.0 41.0 42-43 34.72596744842922 38.0 33.0 40.0 25.0 41.0 44-45 34.58813777183859 38.0 33.0 40.0 25.0 41.0 46-47 34.34266424265026 37.5 33.0 40.0 24.0 41.0 48-49 34.08952609961257 37.0 33.0 40.0 24.0 41.0 50-51 33.84352296427511 37.0 32.0 40.0 23.5 41.0 52-53 33.4759713330709 37.0 31.5 40.0 23.0 41.0 54-55 33.25330604588366 36.5 31.0 39.0 22.5 40.5 56-57 32.85537155302795 36.0 31.0 39.0 21.0 40.0 58-59 32.41522589623 36.0 30.5 39.0 19.5 40.0 60-61 32.121410159460225 35.0 30.0 39.0 19.0 40.0 62-63 31.73007891002134 35.0 30.0 38.0 18.0 40.0 64-65 31.277158177429712 34.5 29.0 38.0 14.5 40.0 66-67 29.502495019026114 33.5 26.5 37.5 10.0 39.5 68-69 25.701519240198618 30.0 17.0 35.0 6.5 39.0 70-71 25.067597081511867 29.5 15.5 35.0 6.0 38.0 72-73 26.983552858750976 31.0 22.0 35.0 6.5 38.0 74-75 27.6413741963108 32.0 25.0 35.0 6.5 38.0 76-77 27.747876827508097 31.5 25.5 34.5 6.5 36.5 78-79 28.61261986277719 33.0 27.0 35.0 2.0 37.0 80-81 28.470538014240233 33.0 27.0 35.0 2.0 37.0 82-83 28.059678023632433 33.0 26.0 35.0 2.0 36.0 84-85 27.719034442096394 32.5 26.0 35.0 2.0 36.0 86-87 27.269640748337373 32.0 26.0 35.0 2.0 35.5 88-89 26.78358013756811 32.0 25.0 34.0 2.0 35.0 90-91 26.334623897625015 32.0 25.0 34.0 2.0 35.0 92-93 25.75276046954096 31.5 23.5 34.0 2.0 35.0 94-95 24.314621661797915 31.0 18.0 34.0 2.0 35.0 96-97 23.49594184432428 30.5 7.0 34.0 2.0 35.0 98-99 23.15380073342035 30.5 2.0 34.0 2.0 35.0 100-101 21.85686044985877 30.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3579974747239554 1101 2 0.2523110362956871 1101 3 0.3361484067767897 1101 4 0.2760712796025828 1101 5 0.3832998058806041 1101 6 0.4609510007863449 1101 7 0.631838855403771 1101 8 0.4951804118256291 1101 9 0.5819230636431314 1101 10-11 0.8108732517249067 1101 12-13 0.7525763421524161 1101 14-15 0.8841558838219683 1101 16-17 0.8698364930927411 1101 18-19 0.9588334576766115 1101 20-21 0.8024628473902382 1101 22-23 0.845868587161668 1101 24-25 0.8015420039432612 1101 26-27 0.9553933732202822 1101 28-29 0.804410410814441 1101 30-31 0.8108871182414603 1101 32-33 0.8611446144321917 1101 34-35 0.9618495081559075 1101 36-37 0.8956278886642082 1101 38-39 0.7742397618346857 1101 40-41 0.9913217723433903 1101 42-43 1.0577323275401156 1101 44-45 0.6308774994542716 1101 46-47 0.7490276957419084 1101 48-49 0.7265645500442375 1101 50-51 0.7879204659470105 1101 52-53 1.0228306107095193 1101 54-55 0.7841128856275219 1101 56-57 0.5211558965176764 1101 58-59 0.6478767060822719 1101 60-61 0.519329307525318 1101 62-63 0.5857929344189294 1101 64-65 0.25144973416293936 1101 66-67 -2.923694902591105 1101 68-69 -8.021049358748474 1101 70-71 -11.558112629294191 1101 72-73 -11.288083981428006 1101 74-75 -9.573703781754187 1101 76-77 -4.540969526002947 1101 78-79 -1.4487820836884033 1101 80-81 -0.7381260737758062 1101 82-83 -0.5006814568039708 1101 84-85 -0.6875912402663715 1101 86-87 -0.6892808932890446 1101 88-89 -0.5070724786209588 1101 90-91 -0.4197046726584972 1101 92-93 -0.9283484821373307 1101 94-95 -4.633212452079164 1101 96-97 -1.5870675685524631 1101 98-99 -0.7079859418555507 1101 100-101 -0.5002718188258761 1102 1 0.4248747135208468 1102 2 0.3599986877301866 1102 3 0.3745694016190342 1102 4 0.24123405771711504 1102 5 0.45090531692198965 1102 6 0.5532889032604018 1102 7 0.5707630330235247 1102 8 0.5833278128827644 1102 9 0.7045676752774881 1102 10-11 0.6652604729594955 1102 12-13 0.6721228465871363 1102 14-15 0.714119808381632 1102 16-17 0.7593347280902236 1102 18-19 0.8173936350088482 1102 20-21 0.8684655793662373 1102 22-23 0.7788482974148678 1102 24-25 0.8390986795693607 1102 26-27 0.8810762115775148 1102 28-29 0.829390842123658 1102 30-31 0.49640057069053256 1102 32-33 0.4575804809134425 1102 34-35 0.6642968113554346 1102 36-37 0.9020424715412219 1102 38-39 0.8483985203731734 1102 40-41 0.8187957472581076 1102 42-43 1.0005224181701848 1102 44-45 0.7923271927790694 1102 46-47 0.901453152189049 1102 48-49 0.749398155896003 1102 50-51 0.761723464838262 1102 52-53 0.8144726573313932 1102 54-55 0.9199221464998146 1102 56-57 0.6591676609372499 1102 58-59 0.6239776465679867 1102 60-61 0.3420381010695408 1102 62-63 0.20731294768545538 1102 64-65 0.311786955199441 1102 66-67 -2.6681899879207798 1102 68-69 -7.973737556351782 1102 70-71 -11.089033990607215 1102 72-73 -9.461717271803607 1102 74-75 -8.022021921407003 1102 76-77 -4.17187147426916 1102 78-79 -1.045877951312928 1102 80-81 -0.4092285933402522 1102 82-83 -0.13669114465010423 1102 84-85 -0.19370663300136215 1102 86-87 0.006698483603091887 1102 88-89 -0.024347118899466835 1102 90-91 -0.006620717739785675 1102 92-93 -0.3151491242960134 1102 94-95 -4.256063787193064 1102 96-97 -0.9578661013109873 1102 98-99 -0.16520868477756778 1102 100-101 0.032997997621780684 1103 1 0.39205409806999825 1103 2 0.277486541120318 1103 3 0.355950889192016 1103 4 0.21401847183973643 1103 5 0.44550592853137516 1103 6 0.40680326867503425 1103 7 0.4684689112944014 1103 8 0.42108031163513715 1103 9 0.61461442719974 1103 10-11 0.6427106099193551 1103 12-13 0.4878048170564995 1103 14-15 0.7402386412838169 1103 16-17 0.6428506580738471 1103 18-19 0.5142350712407904 1103 20-21 0.6759341279253377 1103 22-23 0.688267455060533 1103 24-25 0.794235970852391 1103 26-27 0.6987925457485389 1103 28-29 0.764369220119292 1103 30-31 0.8560668581576891 1103 32-33 0.7600453766915294 1103 34-35 0.6479342745714547 1103 36-37 0.7951815608772606 1103 38-39 0.8853242567700121 1103 40-41 0.9435124606286394 1103 42-43 0.8781501556182576 1103 44-45 0.7125531125852262 1103 46-47 0.6936989531868889 1103 48-49 0.6634104612947809 1103 50-51 0.6095458095123831 1103 52-53 0.5730263765323826 1103 54-55 0.529563877159049 1103 56-57 0.5095059654165013 1103 58-59 0.5584046093918786 1103 60-61 0.5582061338013986 1103 62-63 0.5778459936582045 1103 64-65 0.5150447496716062 1103 66-67 -3.0865090980923036 1103 68-69 -8.040943144251656 1103 70-71 -11.989263814062493 1103 72-73 -9.692560947616432 1103 74-75 -7.949960092240698 1103 76-77 -4.176825377430674 1103 78-79 -1.0779343023677264 1103 80-81 -0.41497001036488257 1103 82-83 -0.21226336067482166 1103 84-85 -0.34361092858420506 1103 86-87 -0.10317093636581376 1103 88-89 -0.06511509473019572 1103 90-91 -0.07707840434573043 1103 92-93 -0.10907037093102545 1103 94-95 0.7969864086183307 1103 96-97 0.9530112007219209 1103 98-99 0.6764639991903785 1103 100-101 0.5955007624710227 1104 1 0.4110579520581332 1104 2 0.16229661997162026 1104 3 0.37275718022112514 1104 4 0.2781062320657526 1104 5 0.40128545223620904 1104 6 0.5060709611272074 1104 7 0.3815133484819384 1104 8 0.3394274153100483 1104 9 0.5878081148267782 1104 10-11 0.6284009803561261 1104 12-13 0.5887530532361751 1104 14-15 0.7188779992038477 1104 16-17 0.7580164606702553 1104 18-19 0.8857799463330096 1104 20-21 0.8755153615572695 1104 22-23 0.7256712031831611 1104 24-25 0.775336132567638 1104 26-27 0.9665740017834068 1104 28-29 0.9813244912324564 1104 30-31 0.9848354530990591 1104 32-33 0.6861657688207643 1104 34-35 0.8244647176626998 1104 36-37 0.9477690404316306 1104 38-39 0.7695250398047833 1104 40-41 0.8974029111760018 1104 42-43 0.9998046313883648 1104 44-45 0.8950518657485631 1104 46-47 0.8166978781682346 1104 48-49 0.8213093583156805 1104 50-51 0.7283806201959777 1104 52-53 0.8613805307929425 1104 54-55 0.7520094983611827 1104 56-57 0.787231095027046 1104 58-59 0.7006502102461951 1104 60-61 0.6144927162736877 1104 62-63 0.6886314687403221 1104 64-65 0.36269845564012826 1104 66-67 -2.8718004807968107 1104 68-69 -7.253095479589177 1104 70-71 -8.979057895697032 1104 72-73 -8.287789671887307 1104 74-75 -7.289203466931301 1104 76-77 -3.6519820590878425 1104 78-79 -0.5626584366433214 1104 80-81 0.04653621101212124 1104 82-83 0.32842961726915476 1104 84-85 0.3233534848120527 1104 86-87 0.4064835280209067 1104 88-89 0.2184106534251633 1104 90-91 0.2925676505634627 1104 92-93 0.5150816200590214 1104 94-95 1.4830587628522736 1104 96-97 1.6565574816624995 1104 98-99 1.446631626686095 1104 100-101 1.6482668189169694 1105 1 0.5418755887873026 1105 2 0.40746185389253853 1105 3 0.4862122704434739 1105 4 0.35532963793735917 1105 5 0.5148635006633739 1105 6 0.6409497518968621 1105 7 0.6438157055341946 1105 8 0.5694765632591583 1105 9 0.7296672570166365 1105 10-11 0.9137304412126639 1105 12-13 0.7561592357725786 1105 14-15 1.0947965641261348 1105 16-17 0.980035165863832 1105 18-19 1.1149447138607442 1105 20-21 1.0610179532194266 1105 22-23 1.0938267947781952 1105 24-25 1.094835254547128 1105 26-27 1.1514321228548354 1105 28-29 1.2734823723940565 1105 30-31 1.0597184131411694 1105 32-33 1.2122636477917297 1105 34-35 1.2727180398308633 1105 36-37 1.2252076231616584 1105 38-39 1.1195623159272188 1105 40-41 1.1606055484175002 1105 42-43 1.218153316830886 1105 44-45 1.0784825409940382 1105 46-47 1.1054305960038917 1105 48-49 0.9662710752079491 1105 50-51 1.0422904437792226 1105 52-53 1.2746419971755287 1105 54-55 1.2568939070488199 1105 56-57 1.3131402967526995 1105 58-59 1.331243130256233 1105 60-61 1.3203982433863217 1105 62-63 1.4168440168371212 1105 64-65 1.4156718888946678 1105 66-67 1.9610014469617063 1105 68-69 -5.230472229888626 1105 70-71 -5.736649328195451 1105 72-73 -1.7650272108859042 1105 74-75 1.1765201668369798 1105 76-77 1.3362263197364967 1105 78-79 1.2202380064019458 1105 80-81 1.241708334832449 1105 82-83 1.4884738539566307 1105 84-85 1.3475349117632192 1105 86-87 1.3840892349775835 1105 88-89 1.4435948257362448 1105 90-91 1.4287088829014678 1105 92-93 1.5699269859833471 1105 94-95 2.490485367757177 1105 96-97 2.5019078595469857 1105 98-99 2.162923235495274 1105 100-101 2.0333379968777727 1106 1 0.12670956353195706 1106 2 0.16391684889059732 1106 3 0.16413644743739653 1106 4 0.0847093617448138 1106 5 0.297539342893991 1106 6 0.31901063672407304 1106 7 0.32456019360963495 1106 8 0.275608835208061 1106 9 0.5152808936230073 1106 10-11 0.4552298128550447 1106 12-13 0.29339253979442503 1106 14-15 0.37328181285250395 1106 16-17 0.3749577514931346 1106 18-19 0.3746041547114629 1106 20-21 0.34336021409642825 1106 22-23 0.46959177122813145 1106 24-25 0.5279242463624527 1106 26-27 0.7178856582119693 1106 28-29 0.7816275247841276 1106 30-31 0.7475959172263842 1106 32-33 0.6045332470312914 1106 34-35 0.8112995536565109 1106 36-37 0.7882822677746901 1106 38-39 0.642314913910063 1106 40-41 0.6927083925812454 1106 42-43 0.8084200039088003 1106 44-45 0.6526664771116089 1106 46-47 0.5463201225462129 1106 48-49 0.28558034463986104 1106 50-51 0.35849851025340485 1106 52-53 0.74005847545358 1106 54-55 0.7186000492890372 1106 56-57 0.6513312676275191 1106 58-59 0.8338341709090074 1106 60-61 0.6600424470097934 1106 62-63 0.7332717414705208 1106 64-65 0.6479552301195604 1106 66-67 1.555397137816879 1106 68-69 -5.5168836848658245 1106 70-71 -6.335239172654692 1106 72-73 -2.468557761882554 1106 74-75 0.42111646931730107 1106 76-77 0.6415091518671083 1106 78-79 0.4901544508590483 1106 80-81 0.621766791671579 1106 82-83 0.687429320667917 1106 84-85 0.6389561392144358 1106 86-87 0.5711368797935421 1106 88-89 0.6076965591488523 1106 90-91 0.57676773847178 1106 92-93 0.7307075022690235 1106 94-95 1.7160440364222431 1106 96-97 1.7921485578961658 1106 98-99 1.583506664654628 1106 100-101 1.5422215139715938 1107 1 -0.10955355204759698 1107 2 -0.04777971727374464 1107 3 0.009489972990920137 1107 4 -0.14225245669818776 1107 5 0.07046952375003457 1107 6 0.0920534812639815 1107 7 0.021754206038743007 1107 8 0.05632439249844623 1107 9 0.1225996709287216 1107 10-11 0.09291950036750052 1107 12-13 0.0952077939157121 1107 14-15 0.07973282408051574 1107 16-17 -0.1568364966422422 1107 18-19 0.18377511395473078 1107 20-21 0.0400773237088643 1107 22-23 0.07847445283439214 1107 24-25 0.37857033272842244 1107 26-27 0.2778149914580652 1107 28-29 0.166370094283252 1107 30-31 0.15644355125142084 1107 32-33 0.059621501503812624 1107 34-35 0.12091522609249239 1107 36-37 0.22785843271191908 1107 38-39 0.13836717241079555 1107 40-41 0.22592357194439217 1107 42-43 0.3499552233435068 1107 44-45 0.16230343380829027 1107 46-47 -0.14843868466275723 1107 48-49 0.026595415851886628 1107 50-51 0.0801761913369532 1107 52-53 0.13168894759564154 1107 54-55 0.002733347424374699 1107 56-57 0.19645269696716383 1107 58-59 0.09704428867942028 1107 60-61 0.033499996191103776 1107 62-63 0.09792159281913726 1107 64-65 0.03126784326270027 1107 66-67 0.7437341150457293 1107 68-69 -6.037931261581292 1107 70-71 -6.80476086342221 1107 72-73 -2.871554179658748 1107 74-75 -0.222467830268819 1107 76-77 -0.0016825546417393866 1107 78-79 0.012081526318894475 1107 80-81 -0.014210904228232124 1107 82-83 0.0921885716123576 1107 84-85 0.19305057665187064 1107 86-87 0.4116703852059871 1107 88-89 0.2960448929973687 1107 90-91 0.18635430486927262 1107 92-93 0.18109419140825977 1107 94-95 1.0740008526879343 1107 96-97 1.0688699694863857 1107 98-99 0.6728677683074729 1107 100-101 0.3427440688951364 1108 1 -0.007913204647188365 1108 2 -0.060037935815209664 1108 3 -0.03473612932392456 1108 4 -0.07479957293639927 1108 5 -0.11301064582438158 1108 6 0.06354940299458889 1108 7 0.19581275047090685 1108 8 0.012047760900955495 1108 9 0.11705118483702392 1108 10-11 0.1449206222102859 1108 12-13 0.15162350620475706 1108 14-15 0.0912340305250865 1108 16-17 0.07317488403564454 1108 18-19 0.002927334577641716 1108 20-21 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2316 98-99 0.6230274552569028 2316 100-101 0.4088975951816245 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 31.0 4 52.0 5 121.0 6 263.0 7 819.0 8 1703.0 9 3073.0 10 4823.0 11 7076.0 12 9498.0 13 12225.0 14 14783.0 15 18097.0 16 21280.0 17 24577.0 18 24414.0 19 22977.0 20 22857.0 21 23295.0 22 25311.0 23 28604.0 24 34995.0 25 44186.0 26 56119.0 27 69840.0 28 85794.0 29 102827.0 30 121397.0 31 140836.0 32 162241.0 33 187888.0 34 217738.0 35 249116.0 36 263553.0 37 219094.0 38 89937.0 39 5372.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.04447551940345 20.03593719711449 15.05032027882844 23.869267004653615 2 16.291085162890127 20.585967584294306 38.75491709577933 24.368030157036237 3 19.612347290419265 23.66558242001091 29.95641432034309 26.76565596922673 4 13.831568842756612 16.938030469402836 35.77828364444997 33.45211704339059 5 14.05475632442885 37.05841221334507 34.31576175862501 14.571069703601072 6 33.219383844034226 35.84225074412469 16.632309169135574 14.306056242705505 7 29.086340909247866 32.01449748275219 20.2546512109723 18.644510397027645 8 26.262120082043634 35.95693399907459 18.971942528021216 18.80900339086056 9 25.655382214211226 16.859388056712316 20.098488615410115 37.386741113666346 10-11 25.34715747819421 26.510283941409245 26.98641152340108 21.156147056995465 12-13 25.854599588400635 23.587587447600498 27.65234701417808 22.905465949820787 14-15 22.680825753965788 25.347438035648924 25.49375953895346 26.47797667143183 16-17 22.56346076526493 28.49809791974337 26.33682456295964 22.601616752032054 18-19 22.397652261778507 27.165966773754814 28.405962060322437 22.030418904144238 20-21 22.982127838837403 26.330301225971596 28.533519393322425 22.15405154186857 22-23 22.77092785961423 26.230266020262288 28.399363609367338 22.59944251075614 24-25 22.705385321924574 26.404276386212803 28.44876330273962 22.441574989123005 26-27 22.559945096862815 26.635639775855353 28.30068963433518 22.503725492946653 28-29 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26.90662774464203 27.35853905909835 22.726685661167593 54-55 22.809837042667784 26.815227203033693 27.491977385513078 22.882958368785445 56-57 22.823719650945392 27.084569316528277 27.409983721723513 22.681727310802817 58-59 22.90529647558522 26.879342167375974 27.377711335270632 22.837650021768173 60-61 22.845750639185813 26.848221481391633 27.65588558153067 22.650142297891882 62-63 22.783997127051983 26.84749635701904 27.667533960869896 22.70097255505908 64-65 22.6886994836679 26.841175866287166 27.52796403200033 22.942160618044603 66-67 22.18022176558977 26.960789307084937 28.01116435278852 22.84782457453677 68-69 22.122641581281005 26.448386617365692 28.800772545393894 22.62819925595941 70-71 21.72888845814172 26.398011109647058 29.077437572212162 22.795662859999062 72-73 22.54879385798805 26.692695540090106 27.755766035328342 23.002744566593506 74-75 22.33279962236779 26.91939264153211 27.67431124052353 23.073496495576567 76-77 22.603462655537175 26.92120572176502 27.345554995320505 23.129776627377304 78-79 22.494168217779727 26.998971776073375 27.3064245433857 23.2004354627612 80-81 22.529405506034113 27.06517284401167 27.264328095976083 23.14109355397813 82-83 22.577796424057844 27.04970960145303 27.232913619381083 23.139580355108045 84-85 22.50640534250454 27.0678163479534 27.104806769290267 23.320971540251794 86-87 22.499629339719696 27.00834746374489 27.287135789856016 23.204887406679394 88-89 22.464335020212115 26.991089231348276 27.037942579075008 23.5066331693646 90-91 22.37804043715045 27.100340954560544 27.196467781542182 23.325150826746828 92-93 22.454074189176357 26.880001553880817 27.243091704866234 23.42283255207659 94-95 21.972939949684996 26.818381696257177 27.608341536396402 23.60033681766143 96-97 21.925044619902888 26.666121558654165 27.70773211921339 23.701101702229558 98-99 22.113725535903843 26.728129895571218 27.1468422068226 24.011302361702338 100-101 22.079592652481217 26.57311475296614 27.33986979452191 24.007422800030735 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 338.0 1 246.5 2 167.5 3 199.5 4 198.0 5 212.0 6 331.0 7 584.0 8 911.0 9 1193.0 10 1298.5 11 1300.0 12 1359.5 13 1493.0 14 1619.5 15 1759.0 16 1997.5 17 2228.5 18 2427.0 19 2688.5 20 3051.0 21 3603.5 22 4315.5 23 5269.0 24 6459.5 25 8077.5 26 10582.5 27 13467.0 28 16335.5 29 19931.5 30 24119.5 31 28685.5 32 33681.0 33 37981.5 34 42032.5 35 46461.0 36 51928.0 37 57249.0 38 61127.0 39 63809.0 40 66568.5 41 70995.5 42 74980.0 43 78420.0 44 81981.5 45 85253.5 46 87743.0 47 90063.5 48 93072.5 49 93627.0 50 92535.0 51 91389.5 52 88827.0 53 86706.5 54 84098.5 55 78336.5 56 71161.5 57 64140.0 58 57233.0 59 49576.5 60 41135.0 61 32746.5 62 25328.5 63 19274.0 64 14221.5 65 10409.5 66 7669.0 67 5728.5 68 4109.5 69 2754.0 70 1892.0 71 1365.5 72 951.5 73 674.0 74 464.5 75 318.0 76 216.0 77 122.0 78 64.5 79 38.0 80 25.5 81 18.5 82 11.0 83 6.5 84 5.0 85 4.0 86 2.0 87 1.0 88 0.5 89 0.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023868964993335684 2 0.017610375618952907 3 0.0 4 0.0 5 2.589761120434251E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 4.5320819607599396E-4 18-19 0.0014243686162388382 20-21 2.1581342670285426E-5 22-23 0.0 24-25 0.0 26-27 3.6688282539485225E-4 28-29 0.01754563159094205 30-31 0.004639988674111366 32-33 0.009236814662882164 34-35 0.009927417628331296 36-37 0.09854041063252325 38-39 0.10115175309562778 40-41 0.11623711162215732 42-43 0.10074170758489237 44-45 0.09659808979219757 46-47 0.09767715692571184 48-49 0.1003964061021678 50-51 0.09854041063252325 52-53 0.07596632619940469 54-55 0.06413975041608828 56-57 0.03651563179812294 58-59 0.015560148065275791 60-61 0.008503049012092458 62-63 0.0013812059308982673 64-65 0.0038846416806513767 66-67 0.42431077824048175 68-69 0.818256607343872 70-71 0.9091572226711142 72-73 0.2776655547958923 74-75 0.1490839151663317 76-77 0.01415736079170724 78-79 0.00863253706811417 80-81 0.001316461902887411 82-83 0.0 84-85 0.0 86-87 1.9423208403256882E-4 88-89 5.395335667571356E-4 90-91 0.003668828253948522 92-93 0.0015538566722605506 94-95 0.2235611287214867 96-97 0.23081245985870263 98-99 0.013337269770236394 100-101 0.007337656507897044 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2316816.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.11009336066883 #Duplication Level Percentage of deduplicated Percentage of total 1 78.40772258303886 47.915032472469264 2 12.741147480488625 15.572254241094202 3 3.9736614778481263 7.284924716849766 4 1.729636439177415 4.227929771125865 5 0.867572607546771 2.6508721522171035 6 0.5213457942308615 1.9115694095183993 7 0.3378247252784429 1.445115035091556 8 0.24340475878618695 1.1899590027083968 9 0.16268718102576818 0.894764593896185 >10 0.9275504038582488 10.158191540843072 >50 0.05683101800050837 2.3704277256267994 >100 0.028980272108783914 3.228234378676557 >500 0.0013508658093787055 0.522638117517011 >1k 1.4219640098704457E-4 0.19225818189461036 >5k 1.4219640098704457E-4 0.4358286604711495 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5028 0.21702198189239025 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5003 0.21594291475887598 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2925 0.12625085462116975 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.316268534057085E-5 2 0.0 0.0 0.0 0.0 4.316268534057085E-5 3 0.0 0.0 0.0 0.0 4.316268534057085E-5 4 0.0 0.0 0.0 0.0 8.63253706811417E-5 5 0.0 0.0 0.0 0.0 8.63253706811417E-5 6 0.0 0.0 0.0 0.0 1.2948805602171256E-4 7 0.0 0.0 0.0 0.0 1.2948805602171256E-4 8 0.0 0.0 0.0 0.0 1.726507413622834E-4 9 0.0 0.0 0.0 0.0 2.1581342670285426E-4 10-11 2.1581342670285426E-5 4.316268534057085E-5 0.0 0.0 2.3739476937313967E-4 12-13 4.316268534057085E-5 4.316268534057085E-5 0.0 8.63253706811417E-5 2.589761120434251E-4 14-15 4.316268534057085E-5 4.316268534057085E-5 0.0 8.63253706811417E-5 3.0213879738399594E-4 16-17 8.63253706811417E-5 4.316268534057085E-5 0.0 8.63253706811417E-5 3.453014827245668E-4 18-19 8.63253706811417E-5 4.316268534057085E-5 0.0 8.63253706811417E-5 3.453014827245668E-4 20-21 8.63253706811417E-5 4.316268534057085E-5 0.0 1.0790671335142713E-4 3.6688282539485225E-4 22-23 8.63253706811417E-5 4.316268534057085E-5 0.0 1.9423208403256885E-4 4.3162685340570857E-4 24-25 8.63253706811417E-5 4.316268534057085E-5 0.0 4.100455107354231E-4 4.747895387462794E-4 26-27 8.63253706811417E-5 4.316268534057085E-5 0.0 7.553469934599899E-4 4.747895387462794E-4 28-29 8.63253706811417E-5 4.316268534057085E-5 0.0 0.0017480887562931196 4.963708814165648E-4 30-31 8.63253706811417E-5 8.63253706811417E-5 0.0 0.00481263941547365 5.179522240868502E-4 32-33 1.0790671335142713E-4 1.726507413622834E-4 0.0 0.01268982949012783 5.611149094274211E-4 34-35 1.2948805602171256E-4 1.726507413622834E-4 0.0 0.024948032126849953 6.04277594767992E-4 36-37 1.2948805602171256E-4 1.726507413622834E-4 0.0 0.04646463076912452 6.258589374382774E-4 38-39 1.726507413622834E-4 1.726507413622834E-4 0.0 0.0755131180033287 6.690216227788481E-4 40-41 1.726507413622834E-4 1.726507413622834E-4 0.0 0.10693555293126429 7.337656507897045E-4 42-43 1.726507413622834E-4 1.726507413622834E-4 0.0 0.14120672509167753 7.337656507897045E-4 44-45 1.726507413622834E-4 1.726507413622834E-4 0.0 0.1846283865442918 7.337656507897045E-4 46-47 1.726507413622834E-4 1.726507413622834E-4 0.0 0.2380853723385888 7.985096788005608E-4 48-49 1.726507413622834E-4 1.726507413622834E-4 0.0 0.29564281324024005 8.63253706811417E-4 50-51 1.726507413622834E-4 1.726507413622834E-4 0.0 0.3594372621736038 8.63253706811417E-4 52-53 1.726507413622834E-4 2.1581342670285426E-4 0.0 0.42090092609857666 8.63253706811417E-4 54-55 1.726507413622834E-4 2.1581342670285426E-4 0.0 0.491320847231718 8.63253706811417E-4 56-57 1.726507413622834E-4 2.1581342670285426E-4 0.0 0.5737399948895381 8.848350494817024E-4 58-59 1.726507413622834E-4 2.1581342670285426E-4 0.0 0.653202498601529 9.279977348222734E-4 60-61 1.726507413622834E-4 2.1581342670285426E-4 0.0 0.7330534664815851 9.495790774925588E-4 62-63 1.726507413622834E-4 2.1581342670285426E-4 0.0 0.8109621135213154 9.927417628331297E-4 64-65 1.726507413622834E-4 2.3739476937313967E-4 0.0 0.8984312953639824 0.0010143231055034152 66-67 1.726507413622834E-4 2.589761120434251E-4 2.1581342670285426E-5 0.989893025600652 0.0010359044481737004 68-69 2.1581342670285426E-4 2.589761120434251E-4 4.316268534057085E-5 1.1028066104515852 0.0010359044481737004 70-71 3.02138797383996E-4 2.589761120434251E-4 4.316268534057085E-5 1.2489554630147581 0.0010359044481737004 72-73 3.453014827245668E-4 2.589761120434251E-4 4.316268534057085E-5 1.4113334852659858 0.0010790671335142712 74-75 3.453014827245668E-4 2.589761120434251E-4 4.316268534057085E-5 1.5897464451212353 0.0010790671335142712 76-77 3.453014827245668E-4 2.589761120434251E-4 4.316268534057085E-5 1.792438415480556 0.0010790671335142712 78-79 3.453014827245668E-4 2.589761120434251E-4 4.316268534057085E-5 2.0243083611301027 0.0010790671335142712 80-81 3.453014827245668E-4 2.589761120434251E-4 4.316268534057085E-5 2.2752346323575114 0.0011006484761845567 82-83 3.453014827245668E-4 2.589761120434251E-4 4.316268534057085E-5 2.534707115282353 0.001208555189535984 84-85 3.453014827245668E-4 2.589761120434251E-4 4.316268534057085E-5 2.786928267069979 0.001208555189535984 86-87 3.453014827245668E-4 2.589761120434251E-4 4.316268534057085E-5 3.0558533780844055 0.0012517178748765547 88-89 3.453014827245668E-4 2.589761120434251E-4 4.316268534057085E-5 3.356977852362898 0.0012732992175468402 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4325 0.0 44.592953 1 GTATCAA 6710 0.0 34.392548 1 ATCAACG 8470 0.0 26.732136 3 TCAACGC 8615 0.0 26.282204 4 TATCAAC 8770 0.0 26.091696 2 CAACGCA 8785 0.0 25.773615 5 AACGCAG 9155 0.0 24.835451 6 GTACATG 7470 0.0 22.646732 1 TACATGG 7535 0.0 21.943048 2 ACGCAGA 10535 0.0 21.402357 7 CGCAGAG 10700 0.0 20.983782 8 ACATGGG 7580 0.0 20.809622 3 GAGTACT 6190 0.0 19.974527 12-13 GTACTTT 6415 0.0 19.27394 14-15 GCAGAGT 11535 0.0 18.561369 9 AGAGTAC 9865 0.0 18.342766 10-11 CGTTATA 160 0.0012126493 17.765854 2 GTGGTAT 2575 0.0 17.666555 1 CAGAGTA 10985 0.0 17.658443 10-11 TATCGTT 220 2.989589E-5 17.224892 7 >>END_MODULE