FastQCFastQC Report
Fri 27 May 2016
SRR939432_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939432_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2285563
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34480.1508599850452602No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28770.12587708148933108No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36100.045.458581
GTATCAA60500.040.977641
ATCAACG78150.031.6929473
TCAACGC79700.030.5674364
TATCAAC80750.030.2531952
CAACGCA81150.030.2318695
AACGCAG83000.029.5944946
ACGCAGA92800.026.0515527
CGCAGAG94400.025.1475328
GTACATG64750.024.879441
TACATGG65550.023.7027192
ACATGGG67550.023.210083
GTGGTAT22150.021.977251
GCAGAGT95950.021.8637149
GAGTACT54800.020.29732112-13
AGAGTAC82250.020.23376710-11
TGGTATC22850.019.8856832
CATGGGA34600.018.8773444
GTACTTT60600.018.71591614-15
GAGTACA48100.018.571641
Produced by FastQC (version 0.11.5)