##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939432_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2285563 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.80572620400313 31.0 26.0 31.0 10.0 33.0 2 26.88117982308954 31.0 26.0 31.0 10.0 34.0 3 27.153632606058114 31.0 26.0 31.0 16.0 34.0 4 30.943150987305973 35.0 30.0 37.0 19.0 37.0 5 31.314476564417607 35.0 30.0 37.0 19.0 37.0 6 31.101354020869255 35.0 30.0 37.0 17.0 37.0 7 31.042685762763924 35.0 30.0 37.0 17.0 37.0 8 30.960803530683688 35.0 30.0 37.0 17.0 37.0 9 32.02283069860687 35.0 30.0 39.0 17.0 39.0 10-11 31.152311487366568 35.0 29.0 38.0 13.5 39.0 12-13 30.887110090599123 35.0 27.0 38.0 11.0 39.0 14-15 31.708088991640132 36.0 27.5 39.0 16.0 40.0 16-17 31.581086148139427 36.0 27.0 38.5 13.5 40.0 18-19 31.44092702760764 35.5 27.0 38.5 11.0 40.0 20-21 31.27267898544035 35.0 27.0 39.0 11.0 40.0 22-23 31.47318450639952 36.0 27.0 39.0 11.0 40.0 24-25 31.552230675767852 36.0 28.0 39.0 11.0 40.0 26-27 31.65984354839486 36.0 29.0 39.0 11.0 40.0 28-29 31.265833626113128 36.0 27.0 39.0 10.0 40.0 30-31 30.989889362052153 35.5 27.0 39.0 10.0 40.0 32-33 30.873719516810517 35.0 27.0 39.0 9.0 40.0 34-35 30.45155307466913 35.0 26.5 38.5 9.0 40.0 36-37 30.106841946601342 35.0 25.5 38.0 9.0 40.0 38-39 29.753139598427172 35.0 25.0 38.0 8.0 40.0 40-41 29.612854907084163 34.5 25.0 38.0 8.0 40.0 42-43 29.36237767237219 34.0 25.0 38.0 7.0 40.0 44-45 29.0516450432563 34.0 24.0 38.0 2.0 40.0 46-47 28.529871633378733 33.5 24.0 38.0 2.0 40.0 48-49 28.2422628472722 33.0 23.0 38.0 2.0 40.0 50-51 27.60417695771239 33.0 22.5 37.5 2.0 39.5 52-53 27.731537262372555 33.0 23.0 38.0 2.0 39.5 54-55 27.85371897427461 33.0 23.0 38.0 2.0 40.0 56-57 27.39232171679363 33.0 21.5 38.0 2.0 40.0 58-59 26.982727669287613 33.0 20.0 38.0 2.0 40.0 60-61 26.366006975086663 32.0 18.0 37.0 2.0 39.5 62-63 25.775982985373844 31.5 14.5 37.0 2.0 39.0 64-65 25.234472862922615 31.0 13.0 36.0 2.0 39.0 66-67 24.853009521067676 31.0 10.0 36.0 2.0 39.0 68-69 24.14161412308477 30.0 8.0 35.0 2.0 38.0 70-71 23.515182473639975 29.5 7.0 35.0 2.0 38.0 72-73 23.01574535464566 29.0 2.0 34.0 2.0 37.5 74-75 22.274657272628232 29.0 2.0 34.0 2.0 36.5 76-77 21.48606317130615 28.0 2.0 34.0 2.0 36.0 78-79 20.953952483480002 27.0 2.0 33.5 2.0 35.5 80-81 20.36723577516787 26.0 2.0 33.0 2.0 35.0 82-83 19.61037302406453 26.0 2.0 33.0 2.0 35.0 84-85 18.890411902887823 25.0 2.0 32.0 2.0 34.5 86-87 18.096197304559094 23.5 2.0 32.0 2.0 34.0 88-89 17.28871989089778 20.0 2.0 31.0 2.0 34.0 90-91 16.468278931711794 17.5 2.0 31.0 2.0 34.0 92-93 15.615523833733747 8.5 2.0 31.0 2.0 34.0 94-95 14.544420346321672 2.0 2.0 30.5 2.0 34.0 96-97 13.452525264015911 2.0 2.0 29.5 2.0 34.0 98-99 12.41146973415303 2.0 2.0 29.0 2.0 34.0 100-101 11.100777138936884 2.0 2.0 27.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 4.046066788289863 1101 2 3.9069258444943813 1101 3 3.4967334764855913 1101 4 3.3774518860827705 1101 5 2.602727192287986 1101 6 2.9506023584300216 1101 7 2.738601968760001 1101 8 2.4542837392623014 1101 9 2.6889421468843935 1101 10-11 3.750773083869145 1101 12-13 3.6215523843290818 1101 14-15 3.1413742416827795 1101 16-17 3.0881491677161144 1101 18-19 3.3029814732008163 1101 20-21 2.956735318620229 1101 22-23 3.1043780340167046 1101 24-25 3.3403032997455426 1101 26-27 3.074239608369414 1101 28-29 3.322235068638811 1101 30-31 3.372477926426665 1101 32-33 3.2581589600322296 1101 34-35 3.3593651706027536 1101 36-37 3.4373217451639704 1101 38-39 3.3776132062999267 1101 40-41 3.1034060792942455 1101 42-43 3.1106147780302216 1101 44-45 3.3292633948237516 1101 46-47 3.505897910553685 1101 48-49 3.253245300477893 1101 50-51 3.3700856240705335 1101 52-53 3.1385473861749524 1101 54-55 3.1579585823896146 1101 56-57 3.3747481540578725 1101 58-59 3.15319511553437 1101 60-61 3.2990074164095837 1101 62-63 3.4050646091683348 1101 64-65 3.2074342484095517 1101 66-67 2.8172392826925083 1101 68-69 2.8330266901893957 1101 70-71 2.7000284420193275 1101 72-73 2.7433733481815317 1101 74-75 2.8713653877298313 1101 76-77 2.8679124430687395 1101 78-79 2.4310731651681614 1101 80-81 2.4813894887178876 1101 82-83 2.0767420940988366 1101 84-85 2.1820051651939103 1101 86-87 2.1403507998371616 1101 88-89 2.0995649153709124 1101 90-91 1.8515788948669147 1101 92-93 1.4686805635781734 1101 94-95 1.4729992795916793 1101 96-97 1.4372341764047647 1101 98-99 1.2339395492421197 1101 100-101 0.9056366431476217 1102 1 3.5211782562258094 1102 2 2.4613858931982406 1102 3 2.853870189323299 1102 4 3.030561826134832 1102 5 1.8660485578802906 1102 6 2.4337853952323663 1102 7 2.481934885645984 1102 8 2.4958038813186363 1102 9 2.488356184983182 1102 10-11 1.8429069396049549 1102 12-13 3.1306198426553316 1102 14-15 3.285497623426405 1102 16-17 3.0187395125550047 1102 18-19 3.047053242781189 1102 20-21 3.044038726456673 1102 22-23 2.971683571878941 1102 24-25 3.088380644258777 1102 26-27 2.731147753055513 1102 28-29 2.9899049637964836 1102 30-31 3.165910995486609 1102 32-33 2.805919155994598 1102 34-35 2.8957071994932697 1102 36-37 3.1054027967413163 1102 38-39 3.2313920563727407 1102 40-41 2.9102086443368336 1102 42-43 2.828737610882591 1102 44-45 2.6801646619842856 1102 46-47 2.9144532410456208 1102 48-49 2.85491102160427 1102 50-51 2.777458175820943 1102 52-53 2.835718138463193 1102 54-55 2.716166657235444 1102 56-57 2.830348893012971 1102 58-59 2.7563334217952153 1102 60-61 3.1203433112354624 1102 62-63 2.9351640240256742 1102 64-65 2.771177318145366 1102 66-67 2.7180215551432916 1102 68-69 2.7453295347978717 1102 70-71 2.7157850005530513 1102 72-73 2.5447658525388697 1102 74-75 2.4200748788379975 1102 76-77 2.4071013333457856 1102 78-79 2.0565096668465834 1102 80-81 2.0065299372931236 1102 82-83 1.8088247882537587 1102 84-85 1.9793792939826425 1102 86-87 1.5427065264319673 1102 88-89 1.39640090543352 1102 90-91 1.366214629736053 1102 92-93 1.1975943323515832 1102 94-95 1.3786473296964399 1102 96-97 1.1353460314319328 1102 98-99 0.7780541241483689 1102 100-101 0.26797502232088455 1103 1 3.765715663277252 1103 2 3.759473230515688 1103 3 3.2673810974550754 1103 4 2.9315412817037974 1103 5 2.631135454392144 1103 6 2.7417123804834453 1103 7 2.530464401554731 1103 8 2.456758622869259 1103 9 2.70481051824666 1103 10-11 3.4155726572147778 1103 12-13 2.6954820728425695 1103 14-15 3.0311426087208453 1103 16-17 2.527141917474683 1103 18-19 2.6262060288893068 1103 20-21 2.5967420931996976 1103 22-23 2.8497358615387967 1103 24-25 2.8901761682754525 1103 26-27 2.920628325091098 1103 28-29 2.4150029888429145 1103 30-31 3.0357976145077252 1103 32-33 2.940398318897561 1103 34-35 2.6118207394512147 1103 36-37 2.8740329030405007 1103 38-39 3.1842746358803424 1103 40-41 2.9293646822894672 1103 42-43 3.02961214026843 1103 44-45 2.9483468086352467 1103 46-47 3.003951011725544 1103 48-49 2.560853153224482 1103 50-51 2.573196597447879 1103 52-53 2.8655548958683674 1103 54-55 2.943624292927691 1103 56-57 3.1221954348569874 1103 58-59 3.037269953016537 1103 60-61 2.941581180048825 1103 62-63 2.9774976921785523 1103 64-65 3.0655549512361375 1103 66-67 2.862001523781057 1103 68-69 3.0143891013631254 1103 70-71 2.7350153973503417 1103 72-73 2.612944964134943 1103 74-75 2.821485312200476 1103 76-77 2.8672757767383956 1103 78-79 2.5008591172848575 1103 80-81 2.1848377496689224 1103 82-83 2.331271837385515 1103 84-85 2.0200804639240353 1103 86-87 2.0621769093547258 1103 88-89 1.9630829400765073 1103 90-91 1.7384875245280433 1103 92-93 1.7246264822543917 1103 94-95 1.7444167522411167 1103 96-97 1.7443154857724217 1103 98-99 1.5575319501819127 1103 100-101 1.3365209419786463 1104 1 3.832900923694666 1104 2 3.5218449529469176 1104 3 2.8764757517815376 1104 4 2.806976359736037 1104 5 1.9367117200021653 1104 6 2.3922346457787604 1104 7 2.4781858028450188 1104 8 2.5213701782213676 1104 9 2.631571786789152 1104 10-11 3.470380473313309 1104 12-13 3.476772644602196 1104 14-15 3.191243027849115 1104 16-17 3.159256727150396 1104 18-19 2.9732442367940948 1104 20-21 2.7378474188466306 1104 22-23 2.5074054797458416 1104 24-25 2.29440278363586 1104 26-27 2.5802645560837014 1104 28-29 2.503962463573629 1104 30-31 2.1662841180052332 1104 32-33 2.310740058637503 1104 34-35 2.9404178756464496 1104 36-37 3.024552736533572 1104 38-39 3.1052839768619265 1104 40-41 3.065756889078486 1104 42-43 3.0378662492989967 1104 44-45 2.9137665806180237 1104 46-47 2.7746480806885927 1104 48-49 2.590352201960659 1104 50-51 2.409022337359474 1104 52-53 2.7777081852385272 1104 54-55 2.597191460736351 1104 56-57 2.813204203649807 1104 58-59 2.8684619652613748 1104 60-61 2.9387961942820233 1104 62-63 3.066940952510137 1104 64-65 2.958670948203533 1104 66-67 2.820442452443423 1104 68-69 2.8413688029666844 1104 70-71 2.704533614410149 1104 72-73 2.544374291670099 1104 74-75 2.7427446996724036 1104 76-77 2.7075667347254395 1104 78-79 2.6114969647107245 1104 80-81 2.5124826207118787 1104 82-83 2.4525485335675867 1104 84-85 2.054209378052807 1104 86-87 2.3570725564286974 1104 88-89 2.1973242892976153 1104 90-91 2.145482389133875 1104 92-93 1.9484608622163417 1104 94-95 2.1938949160873715 1104 96-97 2.4479348658058964 1104 98-99 1.965085271022458 1104 100-101 1.665881950175251 1105 1 3.3748744503186856 1105 2 3.1266174240594324 1105 3 2.7896976353132565 1105 4 2.4962227145722657 1105 5 -0.7483749294427504 1105 6 1.5924044922230074 1105 7 2.1008462215682613 1105 8 2.011184885408298 1105 9 2.325910643457142 1105 10-11 2.5177314586482247 1105 12-13 3.0485600919263725 1105 14-15 2.7571678235215877 1105 16-17 2.6521904905069356 1105 18-19 2.6771731719314857 1105 20-21 2.759105549009032 1105 22-23 2.572279361387686 1105 24-25 2.0586796871217956 1105 26-27 2.3717906237828608 1105 28-29 2.327948025758417 1105 30-31 2.365007349262992 1105 32-33 2.3740025098507367 1105 34-35 2.6101521298632626 1105 36-37 2.481075776836974 1105 38-39 2.6787265161963916 1105 40-41 2.719934030187517 1105 42-43 2.806998407412909 1105 44-45 2.4430635019271314 1105 46-47 2.7085266633237772 1105 48-49 2.669133020242313 1105 50-51 2.7598045447155535 1105 52-53 2.9184181861695926 1105 54-55 2.577653192082881 1105 56-57 2.9325396917255695 1105 58-59 2.81451273182574 1105 60-61 3.012337911911299 1105 62-63 3.036950939735508 1105 64-65 2.8944107864764135 1105 66-67 2.78706765506497 1105 68-69 2.9440874448428254 1105 70-71 3.04395556976662 1105 72-73 2.781934788984188 1105 74-75 2.590869478111564 1105 76-77 2.738660575897903 1105 78-79 2.703381173039503 1105 80-81 2.8292936597476483 1105 82-83 2.8650241221170347 1105 84-85 2.620286989261171 1105 86-87 2.6838452311024916 1105 88-89 2.484758601749146 1105 90-91 2.4334490259024797 1105 92-93 2.47346015379995 1105 94-95 2.381597501489134 1105 96-97 2.3485025531126347 1105 98-99 2.1747299698413993 1105 100-101 2.0204747148439512 1106 1 3.159839442735443 1106 2 2.5838223972435337 1106 3 2.2380469810172237 1106 4 1.3046871593926568 1106 5 1.8490607168545168 1106 6 2.324663705643122 1106 7 2.0648069608381903 1106 8 0.44598114691301305 1106 9 1.7735426230847793 1106 10-11 1.1744780239279429 1106 12-13 1.6463109400329365 1106 14-15 1.7301566311861585 1106 16-17 2.0360105761634735 1106 18-19 2.0377800689744703 1106 20-21 1.9869777587161757 1106 22-23 2.624398514794798 1106 24-25 2.7533403999765085 1106 26-27 2.6164010290041695 1106 28-29 2.0143547251324634 1106 30-31 2.312382299919957 1106 32-33 2.178625321320286 1106 34-35 2.486306460029734 1106 36-37 1.5419221916389887 1106 38-39 2.7288858979022166 1106 40-41 1.8042094073463701 1106 42-43 1.925395122428327 1106 44-45 2.118132433772903 1106 46-47 2.5393566353948778 1106 48-49 2.557139769478791 1106 50-51 2.267900363691176 1106 52-53 2.3633120355791064 1106 54-55 2.3000709561682733 1106 56-57 2.3788909785028274 1106 58-59 2.370238898027914 1106 60-61 2.76915110883526 1106 62-63 2.879122389337354 1106 64-65 2.8216249901466384 1106 66-67 2.406079785289023 1106 68-69 2.4888961125957785 1106 70-71 2.6884241044937234 1106 72-73 2.726526198992886 1106 74-75 2.747833597935742 1106 76-77 2.9417570923539884 1106 78-79 2.83928360887872 1106 80-81 2.7921066726554855 1106 82-83 2.87518344446282 1106 84-85 2.7603711914770805 1106 86-87 2.6841217004860667 1106 88-89 2.879629449143387 1106 90-91 2.6298038742820964 1106 92-93 2.588375105703479 1106 94-95 2.468277702244789 1106 96-97 2.282701801020936 1106 98-99 2.2166127812515857 1106 100-101 1.9771288268622822 1107 1 3.1634973453489046 1107 2 3.5303432939180226 1107 3 -0.16262883303151554 1107 4 1.1124991917183102 1107 5 1.8160151427276432 1107 6 2.2883383066922853 1107 7 2.2427461218205664 1107 8 2.0193817990972036 1107 9 2.393451569350475 1107 10-11 1.7500849175463067 1107 12-13 2.428748439048352 1107 14-15 2.6269618738648397 1107 16-17 1.3246249210649346 1107 18-19 0.34879788589029914 1107 20-21 -1.0682223862136375 1107 22-23 0.7258438746775937 1107 24-25 1.588049516377204 1107 26-27 1.6152864871871877 1107 28-29 2.507969148800136 1107 30-31 3.008611761631709 1107 32-33 1.6869257041240466 1107 34-35 1.3252169658663142 1107 36-37 1.3908627861663163 1107 38-39 2.3949384507149603 1107 40-41 2.91419853760992 1107 42-43 2.0478773606488403 1107 44-45 1.4048894715334228 1107 46-47 2.1460493999311794 1107 48-49 2.1427468206295686 1107 50-51 2.5117085522259686 1107 52-53 2.157824393996851 1107 54-55 2.396317746445373 1107 56-57 2.9271415810643013 1107 58-59 3.0163257929065352 1107 60-61 3.0143739384324526 1107 62-63 2.9616150246970747 1107 64-65 2.1902917933414017 1107 66-67 2.80896814206843 1107 68-69 3.0043027825181206 1107 70-71 1.495677684417796 1107 72-73 2.3759645959430564 1107 74-75 2.9683411673681483 1107 76-77 2.9163904356643755 1107 78-79 2.938688924022312 1107 80-81 2.7426950562154033 1107 82-83 2.4703078163023307 1107 84-85 2.7267529870315137 1107 86-87 2.8455861217907525 1107 88-89 2.8857267411612213 1107 90-91 2.9442716510271616 1107 92-93 2.777916528851291 1107 94-95 2.8358543811464703 1107 96-97 2.680638914081948 1107 98-99 2.7188213856719443 1107 100-101 2.5467699680270766 1108 1 2.2756055556249635 1108 2 2.2967204624783655 1108 3 1.6108884290133894 1108 4 1.5858373216648154 1108 5 1.9272262107204554 1108 6 -0.17018108748558802 1108 7 0.7171598675748214 1108 8 -0.4916265927806087 1108 9 1.4824667214892742 1108 10-11 2.939019065096492 1108 12-13 1.8880571340347991 1108 14-15 2.0019260227486377 1108 16-17 0.04888530142428493 1108 18-19 0.3466200643539423 1108 20-21 1.2538027943590855 1108 22-23 -0.505944005257799 1108 24-25 1.199018311499806 1108 26-27 1.2799712854906993 1108 28-29 2.0469483263401287 1108 30-31 0.8427878774616815 1108 32-33 0.957025955534629 1108 34-35 1.302829816618651 1108 36-37 0.36976701799749634 1108 38-39 1.6708180996292157 1108 40-41 0.6162904152534772 1108 42-43 1.5199842043840768 1108 44-45 2.3811608072694526 1108 46-47 2.1194072870944254 1108 48-49 2.1038188472096095 1108 50-51 2.191808013347881 1108 52-53 1.3140682507372752 1108 54-55 1.625949290817612 1108 56-57 2.493477671078182 1108 58-59 2.279365144342968 1108 60-61 1.587177717988009 1108 62-63 1.640714523686988 1108 64-65 1.3242861427774102 1108 66-67 1.6446580723089887 1108 68-69 2.3768954891415603 1108 70-71 2.4678678377502585 1108 72-73 2.2687202831402153 1108 74-75 2.223000577648161 1108 76-77 2.3477665422743215 1108 78-79 2.1796839962431704 1108 80-81 2.40569275285322 1108 82-83 2.61505025628567 1108 84-85 2.7618327485619076 1108 86-87 2.394494617887876 1108 88-89 2.638759967860466 1108 90-91 2.383096392989682 1108 92-93 2.357242824224974 1108 94-95 2.4931516268258154 1108 96-97 2.5747429489995355 1108 98-99 2.18253198854695 1108 100-101 1.614485523377743 1109 1 1.4130694428507091 1109 2 1.447659338676882 1109 3 0.7527886781607691 1109 4 2.1643368166409935 1109 5 2.081397273793449 1109 6 0.4934160830042238 1109 7 0.4491256811880717 1109 8 -2.255267183788412 1109 9 -0.5475693314806627 1109 10-11 -0.706806154497226 1109 12-13 0.6292096656850497 1109 14-15 0.6098204167518908 1109 16-17 -0.25655764895430266 1109 18-19 0.8845185353768983 1109 20-21 -0.8968681240648912 1109 22-23 -1.4681469864558352 1109 24-25 -0.3670217493061294 1109 26-27 -2.874087138941672 1109 28-29 -2.0196756655698493 1109 30-31 -0.3196081297093265 1109 32-33 -2.462657358646979 1109 34-35 0.2586278841904779 1109 36-37 1.7815998749901354 1109 38-39 2.1078427736010177 1109 40-41 -0.7077188939128298 1109 42-43 -0.254763812909097 1109 44-45 -0.9968463819842874 1109 46-47 -2.1542386097182558 1109 48-49 0.7537014672480673 1109 50-51 0.676637937605495 1109 52-53 0.16304848811997985 1109 54-55 0.8425742206658562 1109 56-57 -0.19941604399544843 1109 58-59 -0.385798899337761 1109 60-61 0.4465526346276718 1109 62-63 -0.9353415990965317 1109 64-65 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54-55 -0.7399149957322102 2312 56-57 -0.13024375096101437 2312 58-59 -0.26203886796728426 2312 60-61 0.02375002404479787 2312 62-63 -0.3831920613063602 2312 64-65 -1.8833171480622966 2312 66-67 1.139845305919149 2312 68-69 0.17470322735972488 2312 70-71 -2.5906286882222638 2312 72-73 -3.1472357728647182 2312 74-75 -0.6177589525817737 2312 76-77 -0.2553643071642995 2312 78-79 -0.38295428067256054 2312 80-81 -1.7766546797418243 2312 82-83 0.16555209683220795 2312 84-85 1.6144348509965063 2312 86-87 1.7764901596678335 2312 88-89 2.1579766870854336 2312 90-91 2.815817300821454 2312 92-93 3.1368476636125155 2312 94-95 2.4351456060121546 2312 96-97 2.0270954753864086 2312 98-99 1.4738681311836554 2312 100-101 2.1083283119752867 2313 1 -7.898076576642449 2313 2 -4.587796141803921 2313 3 -2.1408380584055386 2313 4 -0.12815719649315582 2313 5 0.5592627545857916 2313 6 -2.499361702227212 2313 7 -1.134309659864872 2313 8 -1.8217059883663325 2313 9 0.4786428056149816 2313 10-11 -1.3074480356567761 2313 12-13 -2.198699691881206 2313 14-15 -3.7981547866742034 2313 16-17 -3.4386073753583446 2313 18-19 -0.9719903151334854 2313 20-21 -2.7649548033764972 2313 22-23 -1.4928574895708735 2313 24-25 -2.8101864892529598 2313 26-27 -2.0336759138553013 2313 28-29 -2.628515306251021 2313 30-31 -4.6422312399324674 2313 32-33 -5.95537317745762 2313 34-35 -4.948265033058998 2313 36-37 -2.2719561675545314 2313 38-39 -1.8479990453419717 2313 40-41 -3.5056709548705065 2313 42-43 -2.529005360973553 2313 44-45 -2.8671495262628106 2313 46-47 -1.5634855460188213 2313 48-49 -2.7504360704633335 2313 50-51 -2.9913676964497036 2313 52-53 -0.49211849944557784 2313 54-55 -1.987598218807733 2313 56-57 -2.391071930020896 2313 58-59 -1.4590988079458214 2313 60-61 -2.01875014922231 2313 62-63 -4.468674075890959 2313 64-65 -5.824085027270044 2313 66-67 -4.290730117758795 2313 68-69 -2.565936651680989 2313 70-71 -1.565438406143418 2313 72-73 -1.87139425296278 2313 74-75 -2.654302507291984 2313 76-77 -2.554832645392125 2313 78-79 -2.9751115010680245 2313 80-81 -3.674573096599566 2313 82-83 -1.261347164368459 2313 84-85 -1.0473650157835657 2313 86-87 -2.32318975291855 2313 88-89 -3.311669526136626 2313 90-91 -3.310666815297374 2313 92-93 -1.54976231257087 2313 94-95 -0.6763579377713533 2313 96-97 -0.6986044294689275 2313 98-99 -0.9183481305213004 2313 100-101 -0.7258526044003535 2314 1 -7.9201068745957635 2314 2 -4.226466152339597 2314 3 -24.715260411978885 2314 4 -13.440725612556577 2314 5 -7.298348497203552 2314 6 -5.707549667250273 2314 7 -4.834522948705882 2314 8 -3.7007037073245 2314 9 -4.0156881558168 2314 10-11 -14.448642296910865 2314 12-13 -13.095519949083648 2314 14-15 -5.269274273855029 2314 16-17 -3.4386199626118845 2314 18-19 -3.5028091203166554 2314 20-21 -4.742176938307804 2314 22-23 -4.509134399889149 2314 24-25 -5.3355956690356905 2314 26-27 -4.112626061675957 2314 28-29 -3.627051857175722 2314 30-31 -4.0148596639186245 2314 32-33 -4.29925896968923 2314 34-35 -3.52031345955103 2314 36-37 -4.222177052151988 2314 38-39 -1.3746126950754558 2314 40-41 -2.6513212485586237 2314 42-43 -4.671426662426118 2314 44-45 -5.681457298211871 2314 46-47 -5.053022500024998 2314 48-49 -3.473630645964718 2314 50-51 -2.8983622162199403 2314 52-53 -1.5399612033381054 2314 54-55 -3.9884175477363932 2314 56-57 -3.484282857377398 2314 58-59 -1.7522727258832909 2314 60-61 -4.190868398801218 2314 62-63 -5.183160297478125 2314 64-65 -4.587347579727783 2314 66-67 -5.630429563080487 2314 68-69 -4.945202754033588 2314 70-71 -5.463305286243607 2314 72-73 -4.626411575627909 2314 74-75 -4.15330218233829 2314 76-77 -3.906901888597254 2314 78-79 -4.835432629395221 2314 80-81 -7.109860434661655 2314 82-83 -6.645153214178684 2314 84-85 -5.114458404813327 2314 86-87 -4.3392891395576 2314 88-89 -3.347815343826319 2314 90-91 -4.966462435338247 2314 92-93 -4.947773005659775 2314 94-95 -5.5293153780969195 2314 96-97 -5.040494056906477 2314 98-99 -5.917196613844057 2314 100-101 -4.914130138583428 2315 1 -24.170510751817087 2315 2 -24.33524375061639 2315 3 -13.317717140063754 2315 4 -28.518270039050922 2315 5 -15.677321084065342 2315 6 -12.475122949204831 2315 7 -10.007945256799935 2315 8 -8.814016637078279 2315 9 -6.48005089574152 2315 10-11 -28.7580122484456 2315 12-13 -28.474895285648 2315 14-15 -23.350475018197812 2315 16-17 -14.30357613821256 2315 18-19 -9.520864754798524 2315 20-21 -7.327783574973456 2315 22-23 -4.138004833550909 2315 24-25 -6.039519163730489 2315 26-27 -5.8874907819746305 2315 28-29 -17.3731783792133 2315 30-31 -16.28600131725802 2315 32-33 -9.896706808416159 2315 34-35 -7.8679432798065 2315 36-37 -5.785386205078709 2315 38-39 -6.206808136471992 2315 40-41 -9.057887827639629 2315 42-43 -8.574000472059463 2315 44-45 -5.752530218007397 2315 46-47 -4.7485649222419894 2315 48-49 -5.455777062845385 2315 50-51 -3.8460881912756157 2315 52-53 -7.659632887914281 2315 54-55 -7.51684229578105 2315 56-57 -8.337120010293294 2315 58-59 -8.235714887327742 2315 60-61 -5.762280476241006 2315 62-63 -4.154752236711182 2315 64-65 -5.283534942677878 2315 66-67 -7.702864525543131 2315 68-69 -7.5727485786512005 2315 70-71 -8.336057577587239 2315 72-73 -7.551000865642873 2315 74-75 -7.92956322187451 2315 76-77 -7.745156615832421 2315 78-79 -7.517426335134472 2315 80-81 -8.336493961296807 2315 82-83 -7.264690047510662 2315 84-85 -6.8567549711127835 2315 86-87 -7.138343196415214 2315 88-89 -6.713343847951686 2315 90-91 -6.365785833878245 2315 92-93 -6.14348230065386 2315 94-95 -5.904713279468796 2315 96-97 -5.917927806158932 2315 98-99 -5.813003957869876 2315 100-101 -5.449498147177827 2316 1 -1.8221275913533894 2316 2 1.032053381781413 2316 3 1.2761977942444211 2316 4 1.7758117181180815 2316 5 -1.7195153105703866 2316 6 -3.091540062907029 2316 7 -3.5003452269578226 2316 8 -4.5145561456849705 2316 9 -3.953925250226984 2316 10-11 -3.703710845150905 2316 12-13 -4.431576188637628 2316 14-15 -2.986515535338466 2316 16-17 -3.3608448899435537 2316 18-19 -4.83763639688803 2316 20-21 -2.558538393060907 2316 22-23 -3.02858375350489 2316 24-25 -3.3024128471158924 2316 26-27 -3.0570917527290185 2316 28-29 -2.5027812099897844 2316 30-31 0.011893485206613263 2316 32-33 -2.519481382254778 2316 34-35 -7.205930075012272 2316 36-37 -3.453371750049854 2316 38-39 -1.8178867142296014 2316 40-41 -1.9748466983268713 2316 42-43 -1.8628600298306885 2316 44-45 -1.2958615788997214 2316 46-47 -0.35277283046999486 2316 48-49 0.2927523784768802 2316 50-51 0.6381138847951995 2316 52-53 0.48769448857906283 2316 54-55 -1.4035182353051248 2316 56-57 -0.44408865461312175 2316 58-59 -3.8270244280487695 2316 60-61 -4.043069377700377 2316 62-63 -5.395276088428581 2316 64-65 -5.891967928568622 2316 66-67 -3.6799087528487355 2316 68-69 -3.0976583965359623 2316 70-71 -3.8040309608754477 2316 72-73 -3.844992687677596 2316 74-75 -3.738746076013687 2316 76-77 -3.3958872195662977 2316 78-79 -3.6503443316658384 2316 80-81 -3.87804263068265 2316 82-83 -4.436542292260954 2316 84-85 -3.6054061995960573 2316 86-87 -3.14473284924175 2316 88-89 -4.586266525197994 2316 90-91 -4.582840022181298 2316 92-93 -5.19306619735927 2316 94-95 -6.430646620501121 2316 96-97 -5.7730959593102895 2316 98-99 -5.761283250066743 2316 100-101 -5.329904858382825 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 37174.0 3 13786.0 4 12715.0 5 12981.0 6 14953.0 7 17847.0 8 21582.0 9 25813.0 10 29638.0 11 33772.0 12 36359.0 13 39436.0 14 42024.0 15 44696.0 16 46750.0 17 48637.0 18 48690.0 19 50591.0 20 54618.0 21 58610.0 22 63713.0 23 68719.0 24 74167.0 25 80137.0 26 86235.0 27 92824.0 28 99380.0 29 105497.0 30 112271.0 31 118566.0 32 125130.0 33 128791.0 34 131044.0 35 126363.0 36 107593.0 37 62917.0 38 11472.0 39 72.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.50588122592256 18.716599495015156 14.202573278683664 23.574946000378624 2 16.031868994975415 19.638649494851684 40.1505044310312 24.1789770791417 3 19.897010176627813 23.557884070633456 29.24401246867702 27.30109328406171 4 13.20447239359394 16.215538614804487 36.41572903273079 34.16425995887079 5 14.251983100100048 36.61393737781601 34.22786292342326 14.906216598660682 6 33.15668697202065 34.9355148086027 17.337998747520224 14.56979947185643 7 29.29264087426104 31.781170227161947 20.563562734597014 18.36262616398 8 26.52380499815835 34.89920863879654 19.525323427120885 19.051662935924224 9 26.29547100830436 17.02289327478849 20.38711943445194 36.294516282455206 10-11 25.451549666068107 26.190826425516754 27.142717578697255 21.21490632971788 12-13 26.37459209675621 23.43671351943673 27.56139299830466 22.627301385502392 14-15 23.14387976748481 25.111376887427117 25.675648342757647 26.069095002330428 16-17 22.90603208760824 28.252842803213184 26.350568275995556 22.490556833183017 18-19 22.72446987835198 26.905291297022714 28.53420636237946 21.836032462245843 20-21 23.40665528752341 26.03061154535497 28.626926691698447 21.935806475423174 22-23 23.134163083415974 25.979120650674076 28.4676041935698 22.419112072340152 24-25 22.91672737175416 26.129602093761374 28.7001823014856 22.25348823299887 26-27 22.651655677307396 26.402066176266008 28.559028709623497 22.387249436803096 28-29 22.57460524067161 26.580648396581996 28.59299556088893 22.251750801857465 30-31 22.717135163813843 26.316786018039785 28.537692381359577 22.4283864367868 32-33 22.46251299596947 26.454370863611615 28.506884987998244 22.57623115242067 34-35 23.13195926381841 26.438099068742687 28.205229315732133 22.224712351706767 36-37 22.92245596772434 26.297573761701088 28.2023204372933 22.57764983328127 38-39 22.663317719901492 26.524242703157125 28.463096599789512 22.349342977151874 40-41 22.977796234190855 26.32489740921022 28.37780832430955 22.319498032289378 42-43 22.749589651732922 26.39031074326151 28.19130924642667 22.668790358578896 44-45 22.862698258503393 26.54501503797771 28.073082096559787 22.519204606959107 46-47 22.914614826523852 26.69478344835321 27.67725708257247 22.713344642550464 48-49 22.974357086817133 26.771422205308497 27.62425068591501 22.62997002195936 50-51 22.88246470400181 26.902701521836924 27.627958606947207 22.58687516721406 52-53 22.92464997627608 26.997932238343775 27.451678261984412 22.625739523395733 54-55 22.758476500383086 26.842923290279487 27.491032152756716 22.907568056580715 56-57 22.74774868529531 27.14440423168073 27.48846326505976 22.619383817964202 58-59 22.79682491837983 26.844909407305245 27.560323571625094 22.79794210268983 60-61 22.796256257238422 26.873908213736925 27.756513923176797 22.573321605847855 62-63 22.714800416141927 26.91511799813831 27.732727372282756 22.637354213437003 64-65 22.54372505156685 26.961872727455905 27.61514770961721 22.87925451136004 66-67 22.502874991157665 27.174541255219715 27.31654424639315 23.006039507229474 68-69 22.58473474792057 26.88771316864344 27.62621381654516 22.901338266890832 70-71 22.503351147713335 26.835771859120378 27.550157701068862 23.11071929209742 72-73 22.59903601968968 26.850507696442804 27.490569067329446 23.05988721653807 74-75 22.47214124944081 27.028234903389848 27.387387466333557 23.11223638083579 76-77 22.628537896830483 26.896484930077463 27.321973673513973 23.153003499578084 78-79 22.448047649547412 27.075108471218122 27.307860610787017 23.168983268447448 80-81 22.516176531812935 27.081146698628967 27.20685582563309 23.195820943925007 82-83 22.572607598420177 27.01635432008473 27.19582196003816 23.215216121456933 84-85 22.479367848084795 27.021760290443915 27.105447816993344 23.393424044477946 86-87 22.519933094468463 26.919004654250223 27.290621724142845 23.270440527138476 88-89 22.5147668898322 26.87730583755205 27.07749103843718 23.530436234178566 90-91 22.34267278586007 27.01363322872749 27.194247000067477 23.449446985344956 92-93 22.5073135510012 26.78707171858481 27.26617119558431 23.439443534829678 94-95 22.309720662750642 26.725274792712344 27.2270258695605 23.73797867497651 96-97 22.441542353437804 26.576284143855144 27.150745616361434 23.83142788634561 98-99 22.23794742287929 26.61806615106715 26.904671086091085 24.23931533996247 100-101 22.24172277277656 26.582093626762532 27.15209004167256 24.024093558788348 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1779.0 1 1041.0 2 295.5 3 281.5 4 262.0 5 278.0 6 352.0 7 513.5 8 810.0 9 1054.0 10 1086.5 11 1089.5 12 1141.0 13 1273.5 14 1466.0 15 1633.0 16 1833.0 17 2040.5 18 2289.5 19 2597.5 20 2995.0 21 3569.0 22 4278.0 23 5259.0 24 6547.5 25 8289.0 26 10519.0 27 13214.0 28 16123.0 29 19544.5 30 23574.0 31 28139.0 32 32947.5 33 37162.0 34 41561.0 35 46057.0 36 50888.0 37 56127.5 38 60572.0 39 63637.5 40 66585.5 41 70614.5 42 74460.5 43 78356.0 44 81777.5 45 84623.5 46 86887.0 47 88605.0 48 91002.0 49 91514.5 50 90524.5 51 89582.5 52 86779.0 53 84036.5 54 80969.0 55 75181.0 56 68686.5 57 61889.5 58 55034.5 59 47943.5 60 40199.0 61 32712.0 62 25994.0 63 20176.5 64 15163.5 65 11390.0 66 8594.0 67 6383.0 68 4669.0 69 3361.5 70 2443.5 71 1804.5 72 1303.0 73 914.0 74 647.5 75 445.5 76 325.0 77 228.5 78 153.5 79 107.0 80 74.5 81 54.5 82 33.5 83 25.5 84 25.0 85 17.5 86 10.5 87 5.5 88 4.5 89 6.0 90 3.0 91 1.0 92 1.5 93 2.0 94 1.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 4.08730802870015 2 2.403390324397096 3 0.9472501961223558 4 0.8759329758138366 5 0.15996058739137797 6 0.0915748111078102 7 0.0930186566723385 8 0.09984410843192684 9 0.09560007753013153 10-11 1.696409156081018 12-13 1.2979952860629962 14-15 0.49453898229889093 16-17 0.08897151380206977 18-19 0.09503128988349915 20-21 0.11951103513663809 22-23 0.1342557610531847 24-25 0.14676908927909665 26-27 0.1434876220869869 28-29 0.5358635924715267 30-31 0.17477094265176676 32-33 0.15775106614869072 34-35 0.14081869543740425 36-37 0.13020861818291599 38-39 0.41234916736051463 40-41 0.11843908918721557 42-43 0.12014545212711265 44-45 0.12436760658096058 46-47 0.13384010854218414 48-49 0.14843169932309894 50-51 0.1611200391325901 52-53 0.17984627857556323 54-55 0.1784680623548771 56-57 0.14860671090667815 58-59 0.1329650506242882 60-61 0.11406379959773587 62-63 0.11760778416521445 64-65 0.11393254091005148 66-67 0.10992915093567755 68-69 0.10603514320104061 70-71 0.12088925135732422 72-73 0.13333695023939396 74-75 0.14202190007451118 76-77 0.1626076375930132 78-79 0.16348269551090913 80-81 0.1622357379779074 82-83 0.15471023988400232 84-85 0.14278757575267012 86-87 0.1417812591470898 88-89 0.1389810738098228 90-91 0.1463534367680961 92-93 0.13808413944397946 94-95 0.1860591897926244 96-97 0.18796244076404808 98-99 0.20664492731112644 100-101 0.23125593125194974 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2285563.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.37544035150061 #Duplication Level Percentage of deduplicated Percentage of total 1 86.38607266737701 64.25002194872896 2 8.551895478052488 12.721019840403214 3 2.361234878171687 5.268536515120234 4 0.9584396212587294 2.851374755257739 5 0.5085216740455423 1.891076171770973 6 0.3016137771954419 1.3459594496994232 7 0.18622522328056018 0.9695408090233733 8 0.14144086196859865 0.8415781114088274 9 0.09509651909757079 0.6365560935399044 >10 0.47579410201023686 6.132942793700331 >50 0.022472245711120165 1.1474188346807195 >100 0.010887629426289744 1.4729097953214227 >500 6.106448094982623E-5 0.03869526406728233 >1k 2.442579237993049E-4 0.43236961727761475 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3448 0.1508599850452602 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2877 0.12587708148933108 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 8.750579178959408E-5 0.0 5 0.0 0.0 0.0 8.750579178959408E-5 0.0 6 0.0 0.0 0.0 8.750579178959408E-5 0.0 7 0.0 0.0 0.0 8.750579178959408E-5 0.0 8 0.0 0.0 0.0 8.750579178959408E-5 0.0 9 0.0 0.0 0.0 8.750579178959408E-5 0.0 10-11 0.0 0.0 0.0 1.312586876843911E-4 0.0 12-13 0.0 0.0 0.0 1.312586876843911E-4 0.0 14-15 0.0 2.187644794739852E-5 0.0 1.312586876843911E-4 1.3125868768439113E-4 16-17 0.0 4.375289589479704E-5 0.0 1.312586876843911E-4 1.9688803152658667E-4 18-19 0.0 4.375289589479704E-5 0.0 1.312586876843911E-4 2.406409274213837E-4 20-21 0.0 8.750579178959408E-5 0.0 1.312586876843911E-4 2.625173753687822E-4 22-23 0.0 8.750579178959408E-5 0.0 2.625173753687822E-4 3.500231671583763E-4 24-25 0.0 8.750579178959408E-5 0.0 3.500231671583763E-4 4.3752895894797036E-4 26-27 0.0 8.750579178959408E-5 0.0 6.562934384219555E-4 4.812818548427674E-4 28-29 0.0 8.750579178959408E-5 0.0 0.0011156988453173244 4.812818548427674E-4 30-31 0.0 1.312586876843911E-4 0.0 0.0036096139113207556 4.812818548427674E-4 32-33 4.375289589479704E-5 1.312586876843911E-4 0.0 0.00973501933659234 4.812818548427674E-4 34-35 4.375289589479704E-5 1.312586876843911E-4 0.0 0.019448162225237285 4.812818548427674E-4 36-37 8.750579178959408E-5 1.312586876843911E-4 0.0 0.03749623178184106 4.812818548427674E-4 38-39 1.312586876843911E-4 1.312586876843911E-4 0.0 0.06252288823366497 4.812818548427674E-4 40-41 1.312586876843911E-4 1.312586876843911E-4 0.0 0.09102789990912523 4.812818548427674E-4 42-43 1.312586876843911E-4 1.312586876843911E-4 0.0 0.12073611622169242 5.031583027901658E-4 44-45 1.7501158357918816E-4 1.312586876843911E-4 0.0 0.15963244067216698 5.687876466323614E-4 46-47 1.7501158357918816E-4 1.312586876843911E-4 0.0 0.20837316669897088 6.125405425271585E-4 48-49 1.7501158357918816E-4 1.312586876843911E-4 0.0 0.2595640548958834 6.125405425271585E-4 50-51 1.7501158357918816E-4 1.5313513563178964E-4 0.0 0.3177335299880161 6.125405425271585E-4 52-53 1.7501158357918816E-4 1.7501158357918816E-4 0.0 0.3752904645376216 6.781698863693541E-4 54-55 1.7501158357918816E-4 1.7501158357918816E-4 0.0 0.44037289718113215 7.437992302115497E-4 56-57 1.7501158357918816E-4 1.7501158357918816E-4 0.0 0.5178155229149229 7.437992302115497E-4 58-59 2.1876447947398518E-4 1.7501158357918816E-4 0.0 0.6019085888247229 7.437992302115497E-4 60-61 2.1876447947398518E-4 1.7501158357918816E-4 0.0 0.6913176315857406 7.437992302115497E-4 62-63 2.1876447947398518E-4 1.7501158357918816E-4 0.0 0.7843800411539739 7.875521261063467E-4 64-65 2.1876447947398518E-4 1.7501158357918816E-4 0.0 0.8853617248791654 7.875521261063467E-4 66-67 2.1876447947398518E-4 1.7501158357918816E-4 0.0 1.0004974704263239 7.875521261063467E-4 68-69 2.1876447947398518E-4 1.7501158357918816E-4 0.0 1.136809617586564 7.875521261063467E-4 70-71 2.1876447947398518E-4 1.7501158357918816E-4 0.0 1.2859851161398745 7.875521261063467E-4 72-73 3.0627027126357923E-4 1.7501158357918816E-4 0.0 1.4392077575634539 8.750579178959407E-4 74-75 3.0627027126357923E-4 1.7501158357918816E-4 0.0 1.5924522754349804 8.750579178959407E-4 76-77 3.0627027126357923E-4 1.7501158357918816E-4 0.0 1.7646636736769015 8.750579178959407E-4 78-79 3.0627027126357923E-4 1.7501158357918816E-4 0.0 1.9678083693164443 8.750579178959407E-4 80-81 3.0627027126357923E-4 1.7501158357918816E-4 0.0 2.1890667638564327 8.969343658433392E-4 82-83 3.500231671583763E-4 1.7501158357918816E-4 2.187644794739852E-5 2.4141754132351636 9.188108137907378E-4 84-85 3.500231671583763E-4 1.7501158357918816E-4 4.375289589479704E-5 2.64234676532653 9.188108137907378E-4 86-87 3.9377606305317334E-4 1.7501158357918816E-4 4.375289589479704E-5 2.8856347429495486 9.625637096855348E-4 88-89 3.9377606305317334E-4 1.7501158357918816E-4 4.375289589479704E-5 3.1559838866835 0.0010281930535277304 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3610 0.0 45.45858 1 GTATCAA 6050 0.0 40.97764 1 ATCAACG 7815 0.0 31.692947 3 TCAACGC 7970 0.0 30.567436 4 TATCAAC 8075 0.0 30.253195 2 CAACGCA 8115 0.0 30.231869 5 AACGCAG 8300 0.0 29.594494 6 ACGCAGA 9280 0.0 26.051552 7 CGCAGAG 9440 0.0 25.147532 8 GTACATG 6475 0.0 24.87944 1 TACATGG 6555 0.0 23.702719 2 ACATGGG 6755 0.0 23.21008 3 GTGGTAT 2215 0.0 21.97725 1 GCAGAGT 9595 0.0 21.863714 9 GAGTACT 5480 0.0 20.297321 12-13 AGAGTAC 8225 0.0 20.233767 10-11 TGGTATC 2285 0.0 19.885683 2 CATGGGA 3460 0.0 18.877344 4 GTACTTT 6060 0.0 18.715916 14-15 GAGTACA 4810 0.0 18.57164 1 >>END_MODULE