FastQCFastQC Report
Fri 27 May 2016
SRR939432_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939432_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2285563
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50640.2215646648112522No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46910.20524483464249288No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30050.1314774521638651No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44850.041.3687131
GTATCAA68450.033.4835431
ATCAACG85450.026.9231413
TCAACGC86150.026.7623734
CAACGCA87500.026.1661915
TATCAAC88800.026.0194232
AACGCAG90450.025.4176036
ACGCAGA105700.021.6159087
GTGGTAT26600.021.4070761
CGCAGAG107250.021.2593128
TGGTATC26850.021.2040392
GTACATG72950.020.2298581
TACATGG74050.019.3492242
GCAGAGT116750.019.042219
GAGTACT64400.018.91560412-13
GTACTTT66600.018.29076214-15
AGAGTAC100450.018.238410-11
ACATGGG75150.018.1785623
GTGTAGC11600.017.5900961
CAGAGTA113350.017.29183810-11
Produced by FastQC (version 0.11.5)