##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939432_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2285563 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.858781403094117 31.0 31.0 31.0 28.0 34.0 2 31.113106048706598 31.0 31.0 34.0 28.0 34.0 3 31.13060151918805 31.0 31.0 34.0 28.0 34.0 4 35.04608973806454 35.0 35.0 37.0 32.0 37.0 5 34.59859343190277 35.0 35.0 37.0 30.0 37.0 6 34.64995101863305 35.0 35.0 37.0 30.0 37.0 7 34.489825920353105 35.0 35.0 37.0 30.0 37.0 8 34.38863378519866 35.0 35.0 37.0 30.0 37.0 9 35.905017713359904 37.0 35.0 39.0 30.0 39.0 10-11 35.68840784524426 37.0 35.0 39.0 30.0 39.0 12-13 35.675005458173764 37.0 35.0 39.0 30.0 39.0 14-15 36.552665360788566 38.0 35.0 40.0 30.5 41.0 16-17 36.44859734778696 38.0 35.0 40.0 30.0 41.0 18-19 36.39772738708143 38.0 35.0 40.0 30.0 41.0 20-21 36.24174546927824 38.0 34.0 40.0 29.5 41.0 22-23 36.0930851610741 38.0 34.0 40.0 29.0 41.0 24-25 35.94178939718573 38.0 34.0 40.0 29.0 41.0 26-27 35.47843463514241 38.0 34.0 40.0 27.0 41.0 28-29 35.27192534180856 38.0 33.5 40.0 27.0 41.0 30-31 34.991559410088456 37.5 33.0 40.0 26.5 41.0 32-33 34.81377936202152 37.0 33.0 40.0 25.5 41.0 34-35 35.122225683562434 38.0 33.0 40.0 27.0 41.0 36-37 35.00985271462655 38.0 33.0 40.0 26.0 41.0 38-39 34.84425981694663 38.0 33.0 40.0 25.5 41.0 40-41 34.70970653620137 38.0 33.0 40.0 25.0 41.0 42-43 34.573941081475326 37.0 33.0 40.0 25.0 41.0 44-45 34.236865927563585 37.0 33.0 40.0 24.0 41.0 46-47 33.89985815311151 37.0 32.0 40.0 24.0 41.0 48-49 33.55629378844512 37.0 31.5 40.0 23.0 41.0 50-51 33.32398931904305 37.0 31.0 39.0 22.5 40.5 52-53 33.212620041539 36.5 31.0 39.0 22.5 40.0 54-55 32.876397194039285 36.0 31.0 39.0 21.0 40.0 56-57 32.372246356805746 36.0 30.5 39.0 19.0 40.0 58-59 31.90184256570482 35.0 30.0 39.0 18.0 40.0 60-61 31.52687849777057 35.0 29.0 38.0 15.5 40.0 62-63 31.35127034345586 35.0 29.0 38.0 15.5 40.0 64-65 30.881430308418537 34.0 28.5 38.0 12.5 40.0 66-67 29.9777590904298 34.0 27.0 37.5 9.0 39.5 68-69 26.42673249435697 31.0 19.5 36.0 6.0 39.0 70-71 25.064830415963158 29.5 13.5 35.0 2.0 38.0 72-73 26.107850232087237 30.5 19.5 35.0 3.5 38.0 74-75 26.895708190935885 31.0 23.0 35.0 2.0 37.0 76-77 26.58915571349379 30.5 23.5 34.0 2.0 36.0 78-79 27.751611528538046 32.0 26.0 35.0 2.0 37.0 80-81 27.77445425919128 32.0 26.0 35.0 2.0 36.5 82-83 27.445421325073955 32.0 26.0 35.0 2.0 36.0 84-85 27.083136846370017 32.0 25.5 34.5 2.0 36.0 86-87 26.636205390094258 32.0 25.0 34.0 2.0 35.0 88-89 26.149871825891474 31.5 24.0 34.0 2.0 35.0 90-91 25.55072645120699 31.0 22.0 34.0 2.0 35.0 92-93 24.965317735717633 31.0 20.0 34.0 2.0 35.0 94-95 24.09132957612632 31.0 17.0 34.0 2.0 35.0 96-97 23.413151157942266 30.0 4.5 34.0 2.0 35.0 98-99 22.535906689073983 30.0 2.0 34.0 2.0 35.0 100-101 21.08950661171886 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3034771927478914 1101 2 0.17927045577814482 1101 3 0.2230911722303226 1101 4 0.289375331955668 1101 5 0.45438659383152924 1101 6 0.28778521950273017 1101 7 0.5316465951243146 1101 8 0.7284482032417472 1101 9 0.6706985847775684 1101 10-11 0.6424074444256789 1101 12-13 0.75346548280843 1101 14-15 0.7064962871022047 1101 16-17 0.671681080845083 1101 18-19 0.5239946705864185 1101 20-21 0.5442171069953332 1101 22-23 0.6029384042965091 1101 24-25 0.7233994050519001 1101 26-27 0.6119883377873876 1101 28-29 0.6082578469549276 1101 30-31 0.4377990136220191 1101 32-33 0.6908536433101204 1101 34-35 0.5658880206324142 1101 36-37 0.6966246209976248 1101 38-39 0.7913218611288073 1101 40-41 0.8338609039024902 1101 42-43 0.5357858182537356 1101 44-45 0.6511164990282339 1101 46-47 0.5041196678826552 1101 48-49 0.6428771674890683 1101 50-51 0.4105066757409048 1101 52-53 0.28338730996503614 1101 54-55 0.3680893885661547 1101 56-57 0.6665960056785138 1101 58-59 0.6308770508162667 1101 60-61 0.5202687499124288 1101 62-63 0.38114795218593045 1101 64-65 0.09823211784269148 1101 66-67 -0.28011876630062105 1101 68-69 -7.28900254592428 1101 70-71 -8.330447809556624 1101 72-73 -5.443383829203487 1101 74-75 -3.4893162464588805 1101 76-77 -0.6147506242143592 1101 78-79 -0.3537230079044136 1101 80-81 -0.45064415775214073 1101 82-83 -0.6157451600598272 1101 84-85 -0.5806635439834018 1101 86-87 -0.7589940665867871 1101 88-89 -0.764621518646031 1101 90-91 -1.028667567334569 1101 92-93 -0.9089937522408924 1101 94-95 -0.9001072678171873 1101 96-97 -0.9661564429834186 1101 98-99 -1.0373547989237402 1101 100-101 -0.8388527085712312 1102 1 0.413754157543071 1102 2 0.3672780009848715 1102 3 0.3836664430405712 1102 4 0.34951681482227315 1102 5 0.4899754333156565 1102 6 0.40238675107318755 1102 7 0.4706275006720091 1102 8 0.7147464027555301 1102 9 0.6647478252390471 1102 10-11 0.6086291268593129 1102 12-13 0.5945921845249416 1102 14-15 0.7313167197841821 1102 16-17 0.6197350769987438 1102 18-19 0.6337084074094363 1102 20-21 0.6688599692584489 1102 22-23 0.5906405775372434 1102 24-25 0.536170650523033 1102 26-27 0.6759577227629237 1102 28-29 0.6157738682306118 1102 30-31 0.5873452566866391 1102 32-33 0.6940271769204926 1102 34-35 0.467934483867964 1102 36-37 0.7392036316416153 1102 38-39 0.830397593785996 1102 40-41 0.5214629249692635 1102 42-43 0.5003557066526696 1102 44-45 0.34998922384323805 1102 46-47 0.46142545151709413 1102 48-49 0.5665961310676764 1102 50-51 0.46251514668960425 1102 52-53 0.16075107535908018 1102 54-55 0.002763341013327647 1102 56-57 0.036939390412371154 1102 58-59 0.07374730152439923 1102 60-61 0.165385973014196 1102 62-63 -0.18982747673777212 1102 64-65 -0.2120086979571809 1102 66-67 -0.31900300144727467 1102 68-69 -7.200220408648217 1102 70-71 -8.00065376666528 1102 72-73 -1.6776661846023835 1102 74-75 0.004419122414478949 1102 76-77 0.3868781286760239 1102 78-79 0.2269133168180204 1102 80-81 -0.12334675085372382 1102 82-83 -0.24665470155129654 1102 84-85 -0.4123720499546444 1102 86-87 -0.5391528763939171 1102 88-89 -0.49420757680802296 1102 90-91 -0.6131000485893203 1102 92-93 -0.5826937708786168 1102 94-95 -0.4125218363743208 1102 96-97 -0.7879557208227546 1102 98-99 -0.6836317596227168 1102 100-101 -0.7790947327511191 1103 1 0.25847121093836023 1103 2 0.21224046024648402 1103 3 0.2980569389829917 1103 4 0.294931063682327 1103 5 0.4816901670618492 1103 6 0.3744058574663143 1103 7 0.323898205888689 1103 8 0.4318343398576303 1103 9 0.5847254236434765 1103 10-11 0.43457703953644256 1103 12-13 0.575065580823626 1103 14-15 0.6438657323073969 1103 16-17 0.6348181435603877 1103 18-19 0.6220107277811664 1103 20-21 0.5756909383803759 1103 22-23 0.5270628259348058 1103 24-25 0.5992666232942554 1103 26-27 0.6559090896215665 1103 28-29 0.45591398659774285 1103 30-31 0.43354630739373334 1103 32-33 0.6828398923551902 1103 34-35 0.7319642418584706 1103 36-37 0.6309339742415006 1103 38-39 0.8505210193512696 1103 40-41 0.7272827872355876 1103 42-43 0.36466758022096 1103 44-45 0.7048785527347903 1103 46-47 0.8149653371966892 1103 48-49 0.8951377149327371 1103 50-51 0.7927216749481332 1103 52-53 0.6591286939972107 1103 54-55 0.4683978481420965 1103 56-57 0.8333652519705197 1103 58-59 0.7857991431523459 1103 60-61 0.6171510019556088 1103 62-63 0.29665409975644863 1103 64-65 0.32708606079208735 1103 66-67 0.20733975741761412 1103 68-69 -6.995925733724274 1103 70-71 -8.07033739832524 1103 72-73 -1.2042323813173006 1103 74-75 0.26873887267282015 1103 76-77 0.6040587794785033 1103 78-79 0.36717584906547884 1103 80-81 0.32087320390950325 1103 82-83 0.24405289981592304 1103 84-85 0.006663091645119579 1103 86-87 0.0587595116884323 1103 88-89 0.014374402192284919 1103 90-91 -0.23855960648299046 1103 92-93 -0.16753909147435309 1103 94-95 0.151308475152387 1103 96-97 0.25120060618273143 1103 98-99 0.41120221564881376 1103 100-101 0.535115032899288 1104 1 0.3020874439336225 1104 2 0.26630857931896657 1104 3 0.2691628368186869 1104 4 0.33986876663052357 1104 5 0.46735912725181805 1104 6 0.39135437478218194 1104 7 0.40917378630266654 1104 8 0.595528676619324 1104 9 0.6316943967076938 1104 10-11 0.5291941737290173 1104 12-13 0.5399846876369594 1104 14-15 0.680820829794051 1104 16-17 0.7131692385866373 1104 18-19 0.5715549794297843 1104 20-21 0.6251035866130223 1104 22-23 0.4804805094285882 1104 24-25 0.4336553111223722 1104 26-27 0.7685202730509744 1104 28-29 0.7299145873959461 1104 30-31 0.6140524083564358 1104 32-33 0.6796859878821095 1104 34-35 0.518077649671703 1104 36-37 0.9783057681963214 1104 38-39 0.8360237587049895 1104 40-41 0.7100616219731606 1104 42-43 0.5680158858269735 1104 44-45 0.7998962190629086 1104 46-47 0.8237607367990236 1104 48-49 0.8849380210537348 1104 50-51 0.8831113354971549 1104 52-53 0.5460897994659035 1104 54-55 0.40344226429498775 1104 56-57 0.6440501619223085 1104 58-59 0.7644639406690459 1104 60-61 0.7665580713478057 1104 62-63 0.55632594949968 1104 64-65 0.47409995067435773 1104 66-67 0.567413712815501 1104 68-69 -6.648566241827897 1104 70-71 -4.429269694089609 1104 72-73 0.360077916649999 1104 74-75 1.047488640221136 1104 76-77 1.288311572474914 1104 78-79 0.8673682475377333 1104 80-81 0.577056990940843 1104 82-83 0.45257479318854976 1104 84-85 0.5335365091855664 1104 86-87 0.5436374209106845 1104 88-89 0.442054748938677 1104 90-91 0.481516780717147 1104 92-93 0.5491788337691865 1104 94-95 0.6127698452583843 1104 96-97 0.4010242289958974 1104 98-99 0.5190506428449488 1104 100-101 0.7377712761939499 1105 1 0.08245002590029316 1105 2 0.1849612696531011 1105 3 0.14250666049760596 1105 4 0.25460810698788094 1105 5 0.4078741257280001 1105 6 0.21243933328054254 1105 7 0.10723139530192327 1105 8 0.26882640986036677 1105 9 0.2824535010149205 1105 10-11 0.2993367649369034 1105 12-13 0.1703104608662258 1105 14-15 0.22032299523501564 1105 16-17 0.2959332364608116 1105 18-19 0.3428706503521539 1105 20-21 0.4018356068604305 1105 22-23 0.4387474106533702 1105 24-25 0.43798026548011393 1105 26-27 0.5904346570265417 1105 28-29 0.4739276014387812 1105 30-31 0.43478272493471337 1105 32-33 0.4106888626069036 1105 34-35 0.45912800853520963 1105 36-37 0.5780567236910059 1105 38-39 0.47266614837563736 1105 40-41 0.5498729171954366 1105 42-43 0.4600819981361255 1105 44-45 0.6591241955538933 1105 46-47 0.5812907693167801 1105 48-49 0.5329653850103142 1105 50-51 0.31327029835242115 1105 52-53 0.057377330354306366 1105 54-55 0.07512683221013816 1105 56-57 0.3955452096580103 1105 58-59 0.5705721092015921 1105 60-61 0.4040758612737214 1105 62-63 0.20240039741198856 1105 64-65 0.17863176261842995 1105 66-67 0.34751591561458284 1105 68-69 -3.3977385512479863 1105 70-71 -3.3433533878478556 1105 72-73 0.5249252543633709 1105 74-75 1.0836884286793271 1105 76-77 1.0861859184909264 1105 78-79 0.580124340063275 1105 80-81 0.32255001694290897 1105 82-83 0.2973919899530806 1105 84-85 0.3015072981416722 1105 86-87 0.20975817323154544 1105 88-89 0.21613454496102236 1105 90-91 0.4049606790544793 1105 92-93 0.3838101594215466 1105 94-95 0.3812024060419539 1105 96-97 0.39144253586337285 1105 98-99 0.4435603464146105 1105 100-101 0.3063138872953246 1106 1 0.10402146422372738 1106 2 0.17146665509267933 1106 3 0.16258964583271052 1106 4 0.13136598035510616 1106 5 0.32421861578074385 1106 6 0.23629999152402092 1106 7 0.3097788313934231 1106 8 0.23254941217243896 1106 9 0.4931668009285488 1106 10-11 0.37679493836913025 1106 12-13 0.19953147906897328 1106 14-15 0.3078412113471103 1106 16-17 0.33978620757918776 1106 18-19 0.2741449323182721 1106 20-21 0.3136301498920133 1106 22-23 0.3448082190777484 1106 24-25 0.28407170368483037 1106 26-27 0.6560214161761522 1106 28-29 0.4084784091805247 1106 30-31 0.458384425006237 1106 32-33 0.5329177133668637 1106 34-35 0.36884405978666024 1106 36-37 0.578578053237166 1106 38-39 0.6532609062905621 1106 40-41 0.5347939831830146 1106 42-43 0.2842079957557928 1106 44-45 0.5357646844431869 1106 46-47 0.579562650169315 1106 48-49 0.6225520016958299 1106 50-51 0.4600963170448935 1106 52-53 0.31477952726936564 1106 54-55 0.2878457594555144 1106 56-57 0.3301532998377752 1106 58-59 0.5944703126699977 1106 60-61 0.8085539826471937 1106 62-63 0.33897404689689736 1106 64-65 0.4722809804015142 1106 66-67 0.5919055014892685 1106 68-69 -2.791426798256982 1106 70-71 -2.84657188918494 1106 72-73 0.9002437951748838 1106 74-75 1.3157346879580416 1106 76-77 1.2798924187120413 1106 78-79 0.9575326510623938 1106 80-81 0.7206470824793598 1106 82-83 0.43738819091055703 1106 84-85 0.24010557017092538 1106 86-87 0.34574504872682965 1106 88-89 0.2828458800573479 1106 90-91 0.4097841274572467 1106 92-93 0.20743337750736757 1106 94-95 0.5667691608582821 1106 96-97 0.6894878097654349 1106 98-99 0.893184448965016 1106 100-101 1.0041349286484973 1107 1 0.08433936463173453 1107 2 0.22255441639956786 1107 3 0.2837493994520166 1107 4 -0.11794954131347168 1107 5 0.11283217302014492 1107 6 0.26778757009331144 1107 7 0.16898108725756344 1107 8 0.0761197267646665 1107 9 0.19224051034426282 1107 10-11 -0.002669924026307058 1107 12-13 0.18229191952959667 1107 14-15 0.11868889325766219 1107 16-17 0.14447515983111003 1107 18-19 0.14608604670255687 1107 20-21 0.27579559043140023 1107 22-23 0.3621793181813828 1107 24-25 0.4036230513746162 1107 26-27 0.7372955383267836 1107 28-29 0.45640134432442636 1107 30-31 0.34493351998253274 1107 32-33 0.23980345510823042 1107 34-35 0.23601588038875576 1107 36-37 0.4003679365056456 1107 38-39 0.4994454712647993 1107 40-41 0.3700190182339824 1107 42-43 0.16344994978783234 1107 44-45 0.35501987119786094 1107 46-47 0.42368354054250545 1107 48-49 0.3944891777698061 1107 50-51 0.30738518422932515 1107 52-53 0.1686634888944667 1107 54-55 0.3431782316116525 1107 56-57 0.5650664207123981 1107 58-59 0.6211295228024838 1107 60-61 0.7734415832710262 1107 62-63 0.5177938140639888 1107 64-65 0.2454421833190139 1107 66-67 0.33451644369062805 1107 68-69 -2.7536119303479083 1107 70-71 -3.0296292782427408 1107 72-73 0.9618641735677365 1107 74-75 1.3355924361897387 1107 76-77 0.8805296373197677 1107 78-79 0.8931326132546147 1107 80-81 0.6873478811686873 1107 82-83 0.694948171376538 1107 84-85 0.41127083742466297 1107 86-87 0.5472691748157708 1107 88-89 0.6132858680848834 1107 90-91 0.5978463212536234 1107 92-93 0.492998682568885 1107 94-95 0.8158940683079869 1107 96-97 0.7953385635580297 1107 98-99 1.0480695290702506 1107 100-101 1.431514849567229 1108 1 -0.012425226484431562 1108 2 -0.01923390807320402 1108 3 -0.027669119316517055 1108 4 -0.039580894699284386 1108 5 -0.10468671570227173 1108 6 -0.10327321133753742 1108 7 0.06507969552629334 1108 8 0.2404862692352694 1108 9 0.1858062434275567 1108 10-11 0.2227226271736953 1108 12-13 0.1027601491018757 1108 14-15 0.06951713224754741 1108 16-17 0.15658600440273318 1108 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scores pass #Quality Count 2 6.0 3 28.0 4 69.0 5 133.0 6 345.0 7 881.0 8 1962.0 9 3416.0 10 5160.0 11 7652.0 12 9910.0 13 13021.0 14 15860.0 15 19817.0 16 22889.0 17 25957.0 18 26103.0 19 25038.0 20 25684.0 21 26234.0 22 27908.0 23 31412.0 24 37242.0 25 45963.0 26 58412.0 27 72642.0 28 88962.0 29 106059.0 30 123890.0 31 143609.0 32 164676.0 33 189419.0 34 215299.0 35 240328.0 36 240752.0 37 189717.0 38 74850.0 39 4257.0 40 1.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.88464376270745 20.113230270476542 15.158651039483114 23.8434749273329 2 16.319508413586192 20.718081300628004 38.67138286685516 24.291027418930646 3 19.524122671728037 23.76800827110534 30.103821973470684 26.604047083695935 4 13.754480149407646 17.058613600387094 35.87228536334915 33.31462088685611 5 13.951923442932879 37.15837191974144 34.317802659563526 14.571901977762153 6 33.14618761329265 35.880743606717466 16.64613926634269 14.326929513647185 7 28.948884804312986 32.03630790312934 20.359797564101275 18.655009728456402 8 26.26210697320529 35.96518669579443 19.027959413063652 18.74474691793663 9 25.60533225292849 16.877329568250797 20.113556266005357 37.403781912815354 10-11 25.311163103505464 26.605697878293167 26.966469864709413 21.116669153491962 12-13 25.768683689751715 23.659072184840234 27.675566151534653 22.896677973873395 14-15 22.621887911206123 25.420520020668867 25.521414198602272 26.436177869522737 16-17 22.58146235730182 28.514696356390445 26.342002218722456 22.56183906758528 18-19 22.374397358022822 27.170834644098317 28.49727047320111 21.95749752467775 20-21 23.013323194328926 26.334649274598863 28.590023552183858 22.062003978888352 22-23 22.765327869462133 26.259221485859698 28.421033978995947 22.554416665682226 24-25 22.658011790886345 26.41855546328277 28.48672978358289 22.43670296224799 26-27 22.541532869039706 26.63715133676573 28.33891713728282 22.482398656911744 28-29 22.522438957372763 26.752522605220484 28.399818160657418 22.325220276749327 30-31 22.64344488681198 26.505197532941118 28.281614688420387 22.569742891826515 32-33 22.48554172270735 26.59863612219771 28.248091699911882 22.66773045518306 34-35 22.853577505732908 26.634825194958278 28.12969128269705 22.381906016611765 36-37 22.72741703294598 26.477139244524967 28.166968630434663 22.628475092094387 38-39 22.603427828685643 26.69376919263493 28.305884836307516 22.39691814237191 40-41 22.817148952119407 26.455219436670042 28.3040508825456 22.423580728664955 42-43 22.574036753187467 26.584922369920495 28.03832297572302 22.80271790116902 44-45 22.791173934803734 26.635631875385602 27.98090097080237 22.5922932190083 46-47 22.785446140029812 26.875547468666895 27.60503133693682 22.73397505436647 48-49 22.858592121083156 26.874738935460908 27.614585987763135 22.652082955692805 50-51 22.775860389308306 26.984475173741668 27.583816876448303 22.655847560501723 52-53 23.01454076880832 26.900717790536845 27.4080720080077 22.676669432647135 54-55 22.81072631772699 26.869984883108366 27.448528264341405 22.87076053482324 56-57 22.771328494894124 27.144611154960547 27.439932900518627 22.6441274496267 58-59 22.88028466879048 26.877258027917655 27.429983219123155 22.812474084168713 60-61 22.826066741946065 26.837177928419226 27.71591647268839 22.620838856946317 62-63 22.765738212748083 26.870712135531523 27.68271553082012 22.680834120900276 64-65 22.642622315285777 26.798365548846586 27.595266593755053 22.963745542112584 66-67 22.445369434229676 27.043855647251085 27.570297835900792 22.940477082618447 68-69 22.44903447015708 26.506792354933577 28.38215369437411 22.662019480535225 70-71 21.99497706561277 26.38727376096352 28.841048965330714 22.776700208092997 72-73 22.482741417589512 26.701061963575327 27.847323001130185 22.968873617704972 74-75 22.539692337595987 26.959707248352526 27.396063577784812 23.10453683626667 76-77 21.94718430120171 27.006511206193167 27.88662670765162 23.159677784953505 78-79 22.50572314944208 27.018582102990212 27.330103470691107 23.145591276876594 80-81 22.496967283856762 27.090594259224215 27.26324851208219 23.149189944836834 82-83 22.58273968820751 27.02292010177961 27.27400010457109 23.120340105441787 84-85 22.487413907804154 27.060278702120293 27.13096164460459 23.321345745470964 86-87 22.494483182429843 26.98713176459624 27.31167618277134 23.20670887020258 88-89 22.44024371328708 27.0242735406222 27.06697652582776 23.46850622026296 90-91 22.375718286761646 27.07146398141654 27.184219634185137 23.36859809763668 92-93 22.43764761682239 26.89277691581367 27.260161769222897 23.409413698141044 94-95 22.202203096403366 26.836320547439524 27.366655872540864 23.594820483616246 96-97 22.274749064627784 26.6785269704095 27.373074761495403 23.673649203467313 98-99 22.119296611951366 26.697344517796385 27.160575402247495 24.022783468004754 100-101 21.98824513464841 26.57808181234005 27.39070038439718 24.04297266861436 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 367.0 1 260.5 2 158.0 3 176.5 4 194.0 5 215.0 6 332.0 7 581.5 8 928.5 9 1217.0 10 1299.0 11 1303.0 12 1368.0 13 1476.0 14 1647.5 15 1806.0 16 1960.0 17 2169.0 18 2386.5 19 2679.5 20 3053.5 21 3554.5 22 4245.5 23 5225.5 24 6535.0 25 8210.5 26 10496.0 27 13397.5 28 16372.5 29 19667.5 30 23729.5 31 28344.5 32 33463.0 33 37685.5 34 41510.5 35 45866.5 36 51341.5 37 56703.0 38 60548.5 39 63482.0 40 65959.5 41 70004.5 42 73963.0 43 77525.0 44 81074.5 45 83883.5 46 86108.5 47 88404.0 48 91044.5 49 91735.5 50 91116.5 51 89897.0 52 87367.5 53 85186.5 54 83055.5 55 77285.5 56 69477.5 57 62505.5 58 56188.0 59 49258.0 60 40781.5 61 32379.0 62 25172.5 63 19175.0 64 14150.5 65 10338.0 66 7643.0 67 5715.0 68 4104.0 69 2806.5 70 1985.0 71 1423.0 72 998.5 73 693.5 74 503.0 75 347.5 76 223.5 77 126.5 78 62.5 79 38.0 80 27.5 81 21.5 82 14.5 83 8.5 84 5.0 85 2.0 86 1.5 87 1.0 88 1.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.021220154508976564 2 0.017063629398970843 3 7.000463343167526E-4 4 0.08216793849042883 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.008138038636432249 12-13 0.0 14-15 0.0 16-17 5.9066409457976E-4 18-19 8.750579178959408E-5 20-21 0.0 22-23 1.7501158357918816E-4 24-25 0.0030189498167409955 26-27 0.0036752432551629514 28-29 0.001268833980949114 30-31 3.281467192109778E-4 32-33 9.188108137907379E-4 34-35 0.0032377142962149805 36-37 0.08315237864806177 38-39 0.09341243273539167 40-41 0.07503621645957692 42-43 0.07943338249700402 44-45 0.07796766048452833 46-47 0.076851961639211 48-49 0.07197351374694112 50-51 0.07177662571541454 52-53 0.055194278171286464 54-55 0.04517486501137794 56-57 0.02614235529714123 58-59 0.022817135209136655 60-61 0.013060239424596916 62-63 0.0018813745234762726 64-65 0.006453552144482563 66-67 0.16346081906296173 68-69 0.7478901259777132 70-71 0.9166450454439453 72-73 0.3722277618249858 74-75 0.05714128203860493 76-77 0.2067761859988108 78-79 0.014285320509651233 80-81 0.003084579160583191 82-83 0.001596980700160092 84-85 1.9688803152658664E-4 86-87 0.0022532741385820473 88-89 3.718996151057748E-4 90-91 0.003937760630531733 92-93 0.0037189961510577484 94-95 0.08372116629469413 96-97 0.039858888160160096 98-99 0.013541521279439682 100-101 0.012972733632807322 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2285563.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.02452803383894 #Duplication Level Percentage of deduplicated Percentage of total 1 79.15005807811352 49.09244996145932 2 12.368944032941695 15.343558318403542 3 3.8060559841360977 7.082064782592296 4 1.6167891653929285 4.01122339654883 5 0.8405643866916033 2.606780468329999 6 0.5097137644190128 1.8968853402264385 7 0.33370627858647073 1.4488582101878236 8 0.21700671580475098 1.0767791302370477 9 0.17642719055218062 0.9848531903701654 >10 0.8963234698267826 9.895859716238942 >50 0.05603674067366213 2.3902129685844202 >100 0.026950188463732337 3.095316194937745 >500 0.0011392035180965748 0.44567887063389766 >1k 2.1360065964287522E-4 0.405845107126622 >5k 7.120021988095841E-5 0.22363434412287414 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5064 0.2215646648112522 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4691 0.20524483464249288 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3005 0.1314774521638651 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.375289589479704E-5 2 0.0 8.750579178959408E-5 0.0 0.0 4.375289589479704E-5 3 0.0 8.750579178959408E-5 0.0 0.0 4.375289589479704E-5 4 0.0 8.750579178959408E-5 0.0 0.0 4.375289589479704E-5 5 0.0 8.750579178959408E-5 0.0 0.0 4.375289589479704E-5 6 0.0 8.750579178959408E-5 0.0 0.0 4.375289589479704E-5 7 0.0 8.750579178959408E-5 0.0 0.0 4.375289589479704E-5 8 0.0 8.750579178959408E-5 0.0 0.0 4.375289589479704E-5 9 0.0 8.750579178959408E-5 0.0 0.0 8.750579178959408E-5 10-11 4.375289589479704E-5 8.750579178959408E-5 0.0 4.375289589479704E-5 8.750579178959408E-5 12-13 8.750579178959408E-5 8.750579178959408E-5 0.0 4.375289589479704E-5 1.312586876843911E-4 14-15 8.750579178959408E-5 8.750579178959408E-5 0.0 4.375289589479704E-5 2.1876447947398518E-4 16-17 1.7501158357918816E-4 8.750579178959408E-5 0.0 4.375289589479704E-5 2.625173753687822E-4 18-19 1.7501158357918816E-4 8.750579178959408E-5 0.0 4.375289589479704E-5 3.0627027126357923E-4 20-21 1.9688803152658667E-4 8.750579178959408E-5 0.0 4.375289589479704E-5 3.2814671921097775E-4 22-23 2.1876447947398518E-4 8.750579178959408E-5 0.0 1.5313513563178964E-4 3.9377606305317334E-4 24-25 2.625173753687822E-4 8.750579178959408E-5 0.0 3.062702712635793E-4 4.812818548427674E-4 26-27 2.625173753687822E-4 8.750579178959408E-5 0.0 6.125405425271586E-4 4.812818548427674E-4 28-29 2.625173753687822E-4 8.750579178959408E-5 0.0 0.0013344633247913097 4.812818548427674E-4 30-31 3.0627027126357923E-4 8.750579178959408E-5 0.0 0.004178401557953117 4.812818548427674E-4 32-33 3.0627027126357923E-4 8.750579178959408E-5 0.0 0.010960100421646659 5.031583027901658E-4 34-35 3.0627027126357923E-4 8.750579178959408E-5 0.0 0.022270224010451693 5.250347507375644E-4 36-37 3.0627027126357923E-4 8.750579178959408E-5 0.0 0.04250593836179532 5.46911198684963E-4 38-39 3.500231671583763E-4 8.750579178959408E-5 0.0 0.0706609268700972 6.125405425271585E-4 40-41 3.500231671583763E-4 8.750579178959408E-5 0.0 0.1034537223432476 6.125405425271585E-4 42-43 3.500231671583763E-4 8.750579178959408E-5 0.0 0.1370121934945569 6.125405425271585E-4 44-45 3.500231671583763E-4 8.750579178959408E-5 0.0 0.1817714059949343 6.34416990474557E-4 46-47 3.500231671583763E-4 8.750579178959408E-5 0.0 0.23674691968674677 6.562934384219556E-4 48-49 3.500231671583763E-4 8.750579178959408E-5 0.0 0.29428197778840487 7.437992302115497E-4 50-51 3.500231671583763E-4 8.750579178959408E-5 0.0 0.3583799702742825 7.437992302115497E-4 52-53 3.500231671583763E-4 8.750579178959408E-5 0.0 0.42160290484226426 7.437992302115497E-4 54-55 3.500231671583763E-4 8.750579178959408E-5 0.0 0.4943420942673643 7.437992302115497E-4 56-57 3.500231671583763E-4 8.750579178959408E-5 0.0 0.5799446350855347 7.437992302115497E-4 58-59 3.500231671583763E-4 8.750579178959408E-5 0.0 0.6635564191404919 7.437992302115497E-4 60-61 3.500231671583763E-4 8.750579178959408E-5 0.0 0.7473213383310808 7.875521261063467E-4 62-63 3.9377606305317334E-4 8.750579178959408E-5 0.0 0.8311081339696171 9.188108137907378E-4 64-65 3.9377606305317334E-4 1.312586876843911E-4 0.0 0.9235361265473758 9.406872617381363E-4 66-67 3.9377606305317334E-4 1.312586876843911E-4 0.0 1.0229208295724073 9.625637096855348E-4 68-69 3.9377606305317334E-4 1.312586876843911E-4 0.0 1.1388878801415667 9.625637096855348E-4 70-71 4.3752895894797036E-4 1.312586876843911E-4 0.0 1.2809535331119728 9.625637096855348E-4 72-73 4.3752895894797036E-4 1.312586876843911E-4 0.0 1.4403453328567184 0.001006316605580332 74-75 4.3752895894797036E-4 1.312586876843911E-4 0.0 1.6057531557869986 0.0010500695014751288 76-77 4.3752895894797036E-4 1.312586876843911E-4 0.0 1.8024005463861639 0.0010500695014751288 78-79 4.3752895894797036E-4 1.312586876843911E-4 0.0 2.037069203517908 0.0010500695014751288 80-81 4.3752895894797036E-4 1.312586876843911E-4 0.0 2.2921923394804695 0.001093822397369926 82-83 4.3752895894797036E-4 1.312586876843911E-4 0.0 2.5521939233353006 0.00118132818915952 84-85 4.3752895894797036E-4 1.312586876843911E-4 0.0 2.817358348905718 0.00118132818915952 86-87 4.3752895894797036E-4 1.312586876843911E-4 0.0 3.1007896085122137 0.00118132818915952 88-89 4.3752895894797036E-4 1.312586876843911E-4 0.0 3.420754536190864 0.00118132818915952 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4485 0.0 41.368713 1 GTATCAA 6845 0.0 33.483543 1 ATCAACG 8545 0.0 26.923141 3 TCAACGC 8615 0.0 26.762373 4 CAACGCA 8750 0.0 26.166191 5 TATCAAC 8880 0.0 26.019423 2 AACGCAG 9045 0.0 25.417603 6 ACGCAGA 10570 0.0 21.615908 7 GTGGTAT 2660 0.0 21.407076 1 CGCAGAG 10725 0.0 21.259312 8 TGGTATC 2685 0.0 21.204039 2 GTACATG 7295 0.0 20.229858 1 TACATGG 7405 0.0 19.349224 2 GCAGAGT 11675 0.0 19.04221 9 GAGTACT 6440 0.0 18.915604 12-13 GTACTTT 6660 0.0 18.290762 14-15 AGAGTAC 10045 0.0 18.2384 10-11 ACATGGG 7515 0.0 18.178562 3 GTGTAGC 1160 0.0 17.590096 1 CAGAGTA 11335 0.0 17.291838 10-11 >>END_MODULE