FastQCFastQC Report
Fri 27 May 2016
SRR939431_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939431_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1016984
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46370.45595604257294114No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40070.39400816532020166No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25530.25103639781943476No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19630.19302171912242472No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11570.11376776822447551No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA11040.10855628013813393No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG8500.032.124582
GTACCTG10400.026.8929731
GTACATG57200.024.7101021
GTATCAA87400.024.1720261
TACATGG57700.024.1689952
GGTATCA64300.023.7681081
ACATGGG59400.022.4968073
GTACCCG1352.703809E-422.1973741
GAGTACT46900.021.54666912-13
TATCAAC101750.020.6064132
TCAACGC104100.020.5959914
ATCAACG104050.020.576363
ACCGTGC1455.023201E-420.2521118
CATGGGG18350.020.0821444
CATGGGA42100.020.0071834
CAACGCA104600.019.9801395
ACCTGGG13200.019.9568423
AGTACTT48850.019.65713912-13
AACGCAG106750.019.2582346
CGGGAGT2251.5515325E-619.1432384
Produced by FastQC (version 0.11.5)