FastQCFastQC Report
Fri 27 May 2016
SRR939431_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939431_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1016984
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63450.6239036209025904No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61610.6058109075462347No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42470.41760735665457865No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30450.2994147400549074No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16650.16371938988224002No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA15640.15378806352902308No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15520.15260810396230423No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA14750.14503669674252495No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT13910.13677697977549302No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13540.13313877111144323No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10300.10127986281003437No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG7800.025.5116632
GGTATCA73900.024.0467471
GTACATG66300.023.2294731
TACATGG66950.022.3625132
ACATGGG66400.021.5445563
GTATCAA96700.021.4643211
ATGGGAG15850.020.3225575
GAGTACT54450.019.92215512-13
CATGGGA46900.019.7962044
GTAATAC2001.3153327E-518.9477943
AGTACTT56950.018.79807512-13
GATAGTC2051.6272083E-518.4856537
GTACCTG10850.018.343791
GTATAGG3901.6370905E-1118.2262021
GTACTTT59600.018.16095414-15
TATCAAC114850.018.0687122
ATCAACG114600.018.0632343
TCAACGC114900.018.0160714
CAACGCA115600.017.8250225
GTCGTGA1350.00954976417.5442546
Produced by FastQC (version 0.11.5)