##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939431_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1016984 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.961092799886725 31.0 31.0 31.0 30.0 34.0 2 31.201199822219426 31.0 31.0 34.0 30.0 34.0 3 31.18111887699315 31.0 31.0 34.0 28.0 34.0 4 35.12142668911212 35.0 35.0 37.0 33.0 37.0 5 34.75582801695995 35.0 35.0 37.0 32.0 37.0 6 34.72888462355357 35.0 35.0 37.0 32.0 37.0 7 34.59921493356828 35.0 35.0 37.0 30.0 37.0 8 34.62106188494607 35.0 35.0 37.0 30.0 37.0 9 35.95169737183672 37.0 35.0 39.0 30.0 39.0 10-11 35.81006584174382 37.0 35.0 39.0 30.0 39.0 12-13 35.78202606924003 37.0 35.0 39.0 30.0 39.0 14-15 36.602237105008534 38.0 35.0 40.0 30.0 41.0 16-17 36.6132834931523 38.0 35.0 40.0 30.5 41.0 18-19 36.61080016991418 38.0 35.0 40.0 30.0 41.0 20-21 36.51105081299214 38.0 35.0 40.0 30.0 41.0 22-23 36.36666161906185 38.0 34.5 40.0 30.0 41.0 24-25 36.15785597413529 38.0 34.0 40.0 29.5 41.0 26-27 35.76431438449376 38.0 34.0 40.0 27.5 41.0 28-29 35.485804594762556 38.0 34.0 40.0 27.0 41.0 30-31 35.26117667534592 38.0 34.0 40.0 27.0 41.0 32-33 35.025352906240414 37.0 33.0 40.0 27.0 41.0 34-35 34.950220455779046 37.5 33.0 40.0 26.0 41.0 36-37 34.9201890098566 38.0 33.0 40.0 25.5 41.0 38-39 34.87292769601095 38.0 33.0 40.0 25.0 41.0 40-41 34.65297733297672 38.0 33.0 40.0 25.0 41.0 42-43 34.35386299096152 37.0 33.0 40.0 24.0 41.0 44-45 34.15009233183609 37.0 33.0 40.0 24.0 41.0 46-47 33.80658594432164 37.0 33.0 40.0 23.5 41.0 48-49 33.47746719712404 37.0 32.0 40.0 22.5 41.0 50-51 33.230462819474056 37.0 32.0 40.0 20.5 41.0 52-53 32.86885536055631 37.0 31.0 39.5 19.0 41.0 54-55 32.657211421221966 36.0 31.0 39.0 18.5 40.5 56-57 32.29276615954627 36.0 31.0 39.0 17.5 40.0 58-59 31.880804417768616 35.5 30.0 39.0 14.0 40.0 60-61 31.59870361775603 35.0 30.0 39.0 13.0 40.0 62-63 31.23742654751697 35.0 29.0 38.5 10.5 40.0 64-65 30.79872151380946 34.5 29.0 38.0 8.0 40.0 66-67 29.080852304461033 33.5 25.0 37.5 5.0 39.5 68-69 25.39155679932034 30.0 15.5 35.0 2.0 39.0 70-71 24.769948199775023 29.5 14.0 35.0 2.0 38.0 72-73 26.654060437529008 31.0 21.5 35.0 2.0 38.0 74-75 27.277541731236674 32.0 23.5 35.0 2.0 38.0 76-77 27.326448105378255 31.5 25.5 34.5 2.0 36.5 78-79 28.170454500759107 33.0 26.0 35.0 2.0 37.0 80-81 28.019549471771434 33.0 26.0 35.0 2.0 37.0 82-83 27.607620670531688 33.0 26.0 35.0 2.0 36.0 84-85 27.257658429237825 32.5 26.0 35.0 2.0 36.0 86-87 26.799581409343705 32.0 25.0 35.0 2.0 35.5 88-89 26.29246871140549 32.0 24.5 34.0 2.0 35.0 90-91 25.81661609228857 32.0 23.5 34.0 2.0 35.0 92-93 25.247431621343107 31.5 21.0 34.0 2.0 35.0 94-95 23.85005172156101 31.0 10.0 34.0 2.0 35.0 96-97 23.03879903715299 30.0 4.0 34.0 2.0 35.0 98-99 22.714822947067013 30.0 2.0 34.0 2.0 35.0 100-101 21.470643589279675 29.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4088988315583393 1101 2 0.402143739030528 1101 3 0.4403809275605397 1101 4 0.33893419442139816 1101 5 0.4715161322211472 1101 6 0.5735260016658117 1101 7 0.6182026742472715 1101 8 0.6759912529180099 1101 9 0.7124453804162556 1101 10-11 0.8657750516252207 1101 12-13 0.7949565075603999 1101 14-15 0.7737989294376675 1101 16-17 0.6954295540649085 1101 18-19 0.722984116195768 1101 20-21 0.8471869383053487 1101 22-23 0.7856281807465564 1101 24-25 0.7221007836986928 1101 26-27 1.0667188068336984 1101 28-29 0.8491778247374171 1101 30-31 1.0659103016799278 1101 32-33 0.9374627305564545 1101 34-35 0.8908589367074953 1101 36-37 0.7200896956027449 1101 38-39 0.8560017941347127 1101 40-41 1.125865595022752 1101 42-43 1.1700438868787373 1101 44-45 0.7310788034773239 1101 46-47 0.8892711422208492 1101 48-49 0.8964375858700748 1101 50-51 1.0033544550987301 1101 52-53 1.1115662493891705 1101 54-55 0.819095993066604 1101 56-57 0.7724570767154475 1101 58-59 0.6601651226503158 1101 60-61 0.35045561338947806 1101 62-63 0.3269216511327784 1101 64-65 0.4565195942876983 1101 66-67 -2.6120714467366533 1101 68-69 -7.387530881903384 1101 70-71 -11.013216631507872 1101 72-73 -10.551345789731567 1101 74-75 -8.953105352082119 1101 76-77 -4.000497739057028 1101 78-79 -1.2431718482387453 1101 80-81 -0.5810578597594933 1101 82-83 -0.139096510958872 1101 84-85 -0.2351654204582374 1101 86-87 0.03401896875618249 1101 88-89 0.02008204829348159 1101 90-91 0.06727260995996787 1101 92-93 -0.39955667634934855 1101 94-95 -4.119654356900313 1101 96-97 -0.6723775901913669 1101 98-99 0.09173019334492594 1101 100-101 0.31919663752687555 1102 1 0.44691727873840037 1102 2 0.27594749773460947 1102 3 0.3364605979903672 1102 4 0.10674869575207424 1102 5 0.3280545213132484 1102 6 0.30261950140453564 1102 7 0.31072992576326897 1102 8 0.5258679071488359 1102 9 0.706728486299177 1102 10-11 0.5906946186390414 1102 12-13 0.42581239352328026 1102 14-15 0.3740606594380296 1102 16-17 0.4596765980197475 1102 18-19 0.4156036491831614 1102 20-21 0.4987355201328043 1102 22-23 0.5766824225042697 1102 24-25 0.6359531819536244 1102 26-27 0.7217729452969266 1102 28-29 0.6806054001120145 1102 30-31 0.31214650882791517 1102 32-33 0.15845330949166936 1102 34-35 0.4378378008327104 1102 36-37 0.5526045213996227 1102 38-39 0.5111436569596393 1102 40-41 0.5115459411200405 1102 42-43 0.47237127875371954 1102 44-45 0.26883749540455426 1102 46-47 0.46925411382094495 1102 48-49 0.25952036424871494 1102 50-51 0.21250414400004303 1102 52-53 0.26056626761760526 1102 54-55 0.10578794156518967 1102 56-57 -0.35422411700424306 1102 58-59 -0.2647819389253492 1102 60-61 -0.18811006671357333 1102 62-63 -0.25156430993127543 1102 64-65 -0.37052707962983433 1102 66-67 -3.449457443042352 1102 68-69 -8.300779083364091 1102 70-71 -11.628500114111864 1102 72-73 -9.786827977508793 1102 74-75 -8.080982787097732 1102 76-77 -4.529039676618549 1102 78-79 -1.6905641941172966 1102 80-81 -1.1163576112451103 1102 82-83 -0.7994953795804385 1102 84-85 -1.0046387097192593 1102 86-87 -1.027005666978848 1102 88-89 -0.8250176156956286 1102 90-91 -0.8718075678280748 1102 92-93 -1.3391590229570376 1102 94-95 -5.142647253885329 1102 96-97 -1.720048375472512 1102 98-99 -0.8458093016058008 1102 100-101 -0.5944373992216576 1103 1 0.3691189554238221 1103 2 0.21150892843233393 1103 3 0.37191898504682896 1103 4 0.1329780542848269 1103 5 0.526886843042881 1103 6 0.4194224994731215 1103 7 0.4200377523655874 1103 8 0.38854148928853505 1103 9 0.6126437300980356 1103 10-11 0.5151996237762546 1103 12-13 0.41431445926018284 1103 14-15 0.7307338389816564 1103 16-17 0.6442571684938869 1103 18-19 0.6046984590612894 1103 20-21 0.8454885977852129 1103 22-23 0.6728841755183126 1103 24-25 0.8140776230522491 1103 26-27 0.6064650902584461 1103 28-29 0.5881430013129147 1103 30-31 0.6107684030199749 1103 32-33 0.9351314728453985 1103 34-35 0.7011505977753743 1103 36-37 0.7745427146711847 1103 38-39 0.7308937630339685 1103 40-41 1.1785847721845926 1103 42-43 1.1781798121441156 1103 44-45 0.9890998264058695 1103 46-47 0.9299553493718236 1103 48-49 0.827951594030047 1103 50-51 0.6472843287901426 1103 52-53 0.6658574126741712 1103 54-55 0.5999207398997228 1103 56-57 0.6060292491254486 1103 58-59 0.8446467413303118 1103 60-61 0.5288424456580252 1103 62-63 0.800906006091676 1103 64-65 0.5504032016561062 1103 66-67 -2.455976431284004 1103 68-69 -7.721997841946383 1103 70-71 -11.646194136607855 1103 72-73 -9.616627495141827 1103 74-75 -7.735037894867013 1103 76-77 -3.8802579787257443 1103 78-79 -0.903998854945069 1103 80-81 -0.1567021526634882 1103 82-83 0.05022665701688922 1103 84-85 0.05038711853984168 1103 86-87 0.14850788621584243 1103 88-89 0.3273900659571396 1103 90-91 0.4093406840593765 1103 92-93 0.3725129704996526 1103 94-95 1.177678534637991 1103 96-97 1.292845175282654 1103 98-99 0.7143275511256064 1103 100-101 1.044814819733734 1104 1 0.3960053387716087 1104 2 0.2444664306005926 1104 3 0.4282936598083218 1104 4 0.12690693546436194 1104 5 0.4037298612562381 1104 6 0.3977399322571671 1104 7 0.37753992062231134 1104 8 0.2584460859927802 1104 9 0.7566159764120002 1104 10-11 0.6821553913391298 1104 12-13 0.687081154836946 1104 14-15 0.819198713222768 1104 16-17 0.809911982023813 1104 18-19 0.7087747816978904 1104 20-21 0.9148728128762755 1104 22-23 0.6997705588660992 1104 24-25 0.8600446655500775 1104 26-27 0.9789715950286109 1104 28-29 0.9936070082513382 1104 30-31 0.6597710049280394 1104 32-33 0.8258513340769724 1104 34-35 0.7831107018516903 1104 36-37 0.7658696877848072 1104 38-39 0.9047879521059556 1104 40-41 1.2258527687153773 1104 42-43 1.3551095606263388 1104 44-45 0.9778248914535723 1104 46-47 0.6706318454689608 1104 48-49 0.7173705012286575 1104 50-51 0.45127392115788467 1104 52-53 0.8596995635848401 1104 54-55 0.8648817112787341 1104 56-57 0.7977031433145285 1104 58-59 0.7141176866902335 1104 60-61 1.03664816985577 1104 62-63 0.8538114700986128 1104 64-65 0.6401690299301741 1104 66-67 -2.2933571771643138 1104 68-69 -7.064148752614205 1104 70-71 -8.442811656122167 1104 72-73 -7.913678666000454 1104 74-75 -6.848654547078638 1104 76-77 -3.1244036855774375 1104 78-79 -0.22620180377421306 1104 80-81 0.38753028445706406 1104 82-83 0.438344610182547 1104 84-85 0.3799621402224105 1104 86-87 0.4204072791039586 1104 88-89 0.1946927545954722 1104 90-91 0.1352730344496642 1104 92-93 0.21509753251179475 1104 94-95 1.1174009977776223 1104 96-97 1.4940593990467512 1104 98-99 1.4844923386364464 1104 100-101 1.2941643427172558 1105 1 0.5093139286142687 1105 2 0.4669561655487513 1105 3 0.4955672413547205 1105 4 0.2463516548911997 1105 5 0.40673069900858394 1105 6 0.5537399086168904 1105 7 0.5521440567843996 1105 8 0.5891927789395552 1105 9 0.6898624809753144 1105 10-11 0.7594461711875695 1105 12-13 0.7819817253063945 1105 14-15 0.9829955486760937 1105 16-17 0.9221523268172618 1105 18-19 0.8254709494611134 1105 20-21 1.0543013239377146 1105 22-23 1.0802586123995113 1105 24-25 1.1489909149461397 1105 26-27 1.326010502493176 1105 28-29 1.4110337344718076 1105 30-31 1.1204705207456058 1105 32-33 1.1037047525350019 1105 34-35 1.3662882802303002 1105 36-37 1.2121131737801534 1105 38-39 1.1419531282038662 1105 40-41 1.1859586919347151 1105 42-43 1.2521897647010363 1105 44-45 1.161379089006509 1105 46-47 1.214806659412588 1105 48-49 1.084049308162939 1105 50-51 1.1251924892662615 1105 52-53 1.3363739970677457 1105 54-55 1.2626552403732703 1105 56-57 1.3721980909439182 1105 58-59 1.669052668538935 1105 60-61 1.6767829342139144 1105 62-63 1.6087460086921048 1105 64-65 1.7294886479623877 1105 66-67 2.4266422022783978 1105 68-69 -4.770827574323665 1105 70-71 -5.283523095504798 1105 72-73 -1.8220519046150763 1105 74-75 1.1239267939456674 1105 76-77 1.4706509160764973 1105 78-79 1.5619702265267144 1105 80-81 1.4645990673225917 1105 82-83 1.5530823286888946 1105 84-85 1.4175402085457662 1105 86-87 1.5933794973450937 1105 88-89 1.7486066241986578 1105 90-91 1.6346576484704123 1105 92-93 1.728009187316463 1105 94-95 2.543605654616833 1105 96-97 2.639265372353922 1105 98-99 2.317653664324123 1105 100-101 2.1899369439308884 1106 1 0.11835787252356766 1106 2 0.10260408686954392 1106 3 0.14611135232549088 1106 4 0.06853906639732088 1106 5 0.35965532356976837 1106 6 0.46075858803715875 1106 7 0.22581382045609644 1106 8 0.3301156474824083 1106 9 0.33373611957415505 1106 10-11 0.4307699572949488 1106 12-13 0.25802338729084795 1106 14-15 0.2893892833465159 1106 16-17 0.25329588131601355 1106 18-19 0.2790716860299014 1106 20-21 0.383921472599944 1106 22-23 0.315945639589323 1106 24-25 0.2843414218990432 1106 26-27 0.3677859838624542 1106 28-29 0.26805758305867755 1106 30-31 0.15542457509526741 1106 32-33 0.31445130005612043 1106 34-35 0.30315477638938404 1106 36-37 0.3401571761235047 1106 38-39 0.38671990889185537 1106 40-41 0.3865249778810238 1106 42-43 0.5800434068658546 1106 44-45 0.4441782002095138 1106 46-47 0.43233642706978515 1106 48-49 0.26213346519173797 1106 50-51 0.32825233622896377 1106 52-53 0.603810944472599 1106 54-55 0.5268041483306405 1106 56-57 0.4444840162771797 1106 58-59 0.5477023623594626 1106 60-61 0.5001364048223351 1106 62-63 0.49146735764980676 1106 64-65 0.6151541744157853 1106 66-67 1.1539784033754579 1106 68-69 -5.756310456890688 1106 70-71 -7.09373414733281 1106 72-73 -2.7608377348057083 1106 74-75 0.04262292036700188 1106 76-77 0.24683199572811176 1106 78-79 0.08677623294846626 1106 80-81 0.18027392824204114 1106 82-83 0.2721916887760365 1106 84-85 0.226200510395131 1106 86-87 0.12178893729211282 1106 88-89 0.1869983990807036 1106 90-91 0.20641092489796975 1106 92-93 0.39790808141447087 1106 94-95 1.370455055068419 1106 96-97 1.5014493976813377 1106 98-99 1.2212430886587597 1106 100-101 1.1470272425289174 1107 1 -0.03715461153093713 1107 2 -0.037711545491617926 1107 3 -0.14770073488958602 1107 4 -0.12892333053171257 1107 5 -0.04219470743699816 1107 6 -0.006740899113303556 1107 7 0.0368659047473372 1107 8 0.1285646018588409 1107 9 -0.14718705674469135 1107 10-11 0.020568142161287994 1107 12-13 -0.016142436416245687 1107 14-15 -0.058056254390955075 1107 16-17 -0.1879468886845288 1107 18-19 0.08257603994559304 1107 20-21 0.1080968459190359 1107 22-23 0.10376387156708233 1107 24-25 0.3494025747847971 1107 26-27 0.32603295805020593 1107 28-29 0.19678564547776745 1107 30-31 0.10145865659944064 1107 32-33 0.39250110423013496 1107 34-35 0.15201507146060322 1107 36-37 0.23904918533829544 1107 38-39 0.06534009606425428 1107 40-41 0.040713484864035365 1107 42-43 0.25683502018437565 1107 44-45 0.21052631068319982 1107 46-47 -0.11064743762282347 1107 48-49 0.27308132902606275 1107 50-51 0.2813248354685314 1107 52-53 0.3375736566567227 1107 54-55 0.3876474380187105 1107 56-57 0.25225268458968486 1107 58-59 0.07731677024814942 1107 60-61 -0.016997485453803307 1107 62-63 -6.064643878680442E-4 1107 64-65 -0.06068129873801453 1107 66-67 0.8535428939095517 1107 68-69 -5.652820957926998 1107 70-71 -5.975394412222542 1107 72-73 -2.6364487331292175 1107 74-75 -0.02498361675505123 1107 76-77 0.16291920137016191 1107 78-79 0.2219162113969979 1107 80-81 0.1657856341800148 1107 82-83 0.13410312025108695 1107 84-85 0.10644130209333369 1107 86-87 0.32668588400999354 1107 88-89 0.18511677182682007 1107 90-91 0.2259817346814863 1107 92-93 0.38891847752294595 1107 94-95 1.1830044242045474 1107 96-97 1.249962134034238 1107 98-99 0.8036562402429333 1107 100-101 0.6966427822234742 1108 1 0.07992858791400437 1108 2 -0.18427976622281506 1108 3 0.016875674215786063 1108 4 0.004324793831813167 1108 5 0.022807107050446973 1108 6 0.21207509587327422 1108 7 -0.0064571811282760905 1108 8 -0.09798894310607409 1108 9 0.24523941654717163 1108 10-11 0.30746938128834955 1108 12-13 0.03455690061640837 1108 14-15 -4.159033875552609E-4 1108 16-17 0.02790517703348172 1108 18-19 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1.9038843066297382 2310 100-101 1.7467792006857898 2311 1 -0.017883653233681684 2311 2 0.013302422649754675 2311 3 0.017352484528810663 2311 4 -0.16452870358923377 2311 5 -0.1646124094569359 2311 6 0.0768210638678184 2311 7 -0.1495194170554086 2311 8 0.05550569364799429 2311 9 0.011066386609051904 2311 10-11 -1.2262623380826767 2311 12-13 -0.20286505214805572 2311 14-15 0.2597589502630058 2311 16-17 0.2520895529688332 2311 18-19 0.26838370242911935 2311 20-21 0.36581157523196595 2311 22-23 0.1291469783571202 2311 24-25 0.12054299337699348 2311 26-27 0.36258936089738114 2311 28-29 0.1558326858104735 2311 30-31 0.3032695251139259 2311 32-33 0.4601394034341837 2311 34-35 0.4183103670796058 2311 36-37 0.7095776936298179 2311 38-39 0.4123620862375219 2311 40-41 0.5301169788118685 2311 42-43 0.6254903301296864 2311 44-45 0.5576804366224977 2311 46-47 0.5349474862774102 2311 48-49 0.7568547092328188 2311 50-51 0.6962919927761604 2311 52-53 0.9907689950459826 2311 54-55 0.8932879835307901 2311 56-57 0.9750640121020879 2311 58-59 1.1727887731134068 2311 60-61 1.252496017291488 2311 62-63 1.5676273150775337 2311 64-65 1.4485508185985445 2311 66-67 2.6045069634129625 2311 68-69 4.888886131852303 2311 70-71 5.372706396935161 2311 72-73 3.443083088988221 2311 74-75 2.5420805003867812 2311 76-77 1.833060517094669 2311 78-79 1.3134384849667136 2311 80-81 0.891706498765199 2311 82-83 1.1225321129245067 2311 84-85 1.2537539909518571 2311 86-87 1.1013331162872824 2311 88-89 1.2621440995895874 2311 90-91 0.8978868890195351 2311 92-93 1.2969585042282823 2311 94-95 1.8404863511612852 2311 96-97 2.1547052864458855 2311 98-99 1.9792151223743524 2311 100-101 1.9502404392477075 2312 1 -0.1627658921980384 2312 2 -0.10252760654632453 2312 3 -0.02743683382459494 2312 4 -0.047409136937169194 2312 5 0.1745148231455218 2312 6 0.17651537617610558 2312 7 0.20206234516285804 2312 8 0.1386300435996901 2312 9 -1.4580031929779764 2312 10-11 -0.9806074325607668 2312 12-13 -2.075884549637607 2312 14-15 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0.9921267533465503 2312 82-83 1.317160536072297 2312 84-85 1.2273145134191097 2312 86-87 1.2260308603226768 2312 88-89 1.2191696369188563 2312 90-91 1.3726927324588694 2312 92-93 1.6102745293379641 2312 94-95 2.4481798873712854 2312 96-97 2.907849276435801 2312 98-99 2.4279464392600367 2312 100-101 2.2220246469675082 2313 1 -0.17345972657009412 2313 2 -0.19381122806014872 2313 3 -0.1659178822474594 2313 4 0.11032738406775167 2313 5 0.06664836630360327 2313 6 0.10516072526162645 2313 7 -3.4940257812313646 2313 8 -0.9780345970973343 2313 9 -0.9271996934165756 2313 10-11 0.1868956310338703 2313 12-13 -1.257928599693301 2313 14-15 -0.4460945841864543 2313 16-17 -0.03355878273556101 2313 18-19 0.1973355212361767 2313 20-21 -0.17196718132770883 2313 22-23 -1.1629126404518217 2313 24-25 -2.6010926139754886 2313 26-27 -2.163484446585123 2313 28-29 -0.4287258259804503 2313 30-31 -0.2604770478409222 2313 32-33 -2.766727452917273 2313 34-35 -2.514001369195725 2313 36-37 -1.0604004486526222 2313 38-39 -0.29437824030923565 2313 40-41 0.010717076794740876 2313 42-43 -0.05396435750280659 2313 44-45 0.2399839291465966 2313 46-47 -0.14549998453971114 2313 48-49 0.31613905741118487 2313 50-51 0.2354898522272677 2313 52-53 0.73869981348561 2313 54-55 0.762324218350507 2313 56-57 0.8628500702699995 2313 58-59 0.3880696259412453 2313 60-61 1.0695977969996129 2313 62-63 0.5854868132921744 2313 64-65 0.6527296938682348 2313 66-67 1.9362449346022323 2313 68-69 5.0824894519642285 2313 70-71 4.9650022581009665 2313 72-73 3.042026946686942 2313 74-75 2.084008219175935 2313 76-77 1.6098998157634163 2313 78-79 1.4501693429763414 2313 80-81 1.3171076161467923 2313 82-83 1.22258416632517 2313 84-85 1.3378979598234508 2313 86-87 1.1856160512511522 2313 88-89 1.3608736197561555 2313 90-91 1.0114834257018757 2313 92-93 0.9086719325924371 2313 94-95 2.0239722331484558 2313 96-97 2.199537161027809 2313 98-99 1.921742044110534 2313 100-101 1.809567890468255 2314 1 -0.22327283924316887 2314 2 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-3.051943612060306 2314 62-63 -1.9077723136266513 2314 64-65 -0.9443400538926454 2314 66-67 -0.7721990814771296 2314 68-69 1.5091214837468279 2314 70-71 1.5708227992514416 2314 72-73 -0.5072102239094498 2314 74-75 -0.6762176911651601 2314 76-77 -0.7900566947328329 2314 78-79 -0.39802878109803785 2314 80-81 0.04278287884690357 2314 82-83 -0.09109857186802017 2314 84-85 -0.4519772135468685 2314 86-87 -0.43921057842984723 2314 88-89 -0.43874555323803577 2314 90-91 -0.08752571884783222 2314 92-93 0.09734585534567586 2314 94-95 -0.06220163083518315 2314 96-97 0.3953986058196506 2314 98-99 0.02611685310933609 2314 100-101 -0.09274528097442314 2315 1 -0.34170154489392246 2315 2 -0.34494768045411917 2315 3 -0.23168371837961743 2315 4 0.017953200933590097 2315 5 -1.3486319065491088 2315 6 -0.3388758286710498 2315 7 -0.2606429803253292 2315 8 -0.04540162045123708 2315 9 0.16917094888080442 2315 10-11 -1.6459234998989771 2315 12-13 -1.1595987811611792 2315 14-15 -0.24651245499798335 2315 16-17 -1.7371308621961035 2315 18-19 -3.2097500576646 2315 20-21 -2.4632205334066484 2315 22-23 -1.8747575205580418 2315 24-25 -1.3820293735891198 2315 26-27 -0.5707457655468176 2315 28-29 -0.5788534002468992 2315 30-31 -2.9236419310714155 2315 32-33 -2.5578701538467357 2315 34-35 -1.693594693676964 2315 36-37 -4.45058894614019 2315 38-39 -1.7720332170074116 2315 40-41 -1.5389783070996614 2315 42-43 -1.0813928247891198 2315 44-45 -1.2921114130909004 2315 46-47 -1.451183548287787 2315 48-49 -4.457117210650992 2315 50-51 -3.8074167582025815 2315 52-53 -2.249994242594994 2315 54-55 -4.0098190293258185 2315 56-57 -6.442436451118219 2315 58-59 -7.052543251248995 2315 60-61 -6.227461485980935 2315 62-63 -6.781230622725424 2315 64-65 -6.083796901460985 2315 66-67 -4.021994019809494 2315 68-69 -1.7198615595174545 2315 70-71 0.9383678755343823 2315 72-73 -0.8312449427139761 2315 74-75 -2.4905511288935216 2315 76-77 -4.849569319074771 2315 78-79 -3.881758069869605 2315 80-81 -4.0266920141481926 2315 82-83 -7.350803581338138 2315 84-85 -4.881764098548288 2315 86-87 -5.388884716798003 2315 88-89 -3.888807444577722 2315 90-91 -3.455965609680458 2315 92-93 -4.0085479898215794 2315 94-95 -2.4005832095863227 2315 96-97 -0.9980662652524863 2315 98-99 -2.849496467696593 2315 100-101 -1.728496104949489 2316 1 -0.38906415296034425 2316 2 -0.45047382586296436 2316 3 -1.0515641312219053 2316 4 -0.5019792615208658 2316 5 -0.27455388291097194 2316 6 -2.324015029646027 2316 7 -0.4386678879690038 2316 8 -0.3449027058760734 2316 9 0.08365216658782515 2316 10-11 -0.12262050768102739 2316 12-13 -0.9566789248688963 2316 14-15 -1.3123653704110154 2316 16-17 -1.7270852870478919 2316 18-19 -0.4897465030569421 2316 20-21 -3.964159965431122 2316 22-23 -4.537635991061151 2316 24-25 -3.0450043263001447 2316 26-27 -2.2025455395753326 2316 28-29 -1.3952807656478683 2316 30-31 -0.2894186763203024 2316 32-33 -1.9728147400523213 2316 34-35 -2.3114159730818216 2316 36-37 -4.963184952284582 2316 38-39 -2.3332042063308904 2316 40-41 -1.5895400620602445 2316 42-43 -3.191080400165845 2316 44-45 -0.7922624839164598 2316 46-47 -0.6876487448702875 2316 48-49 -0.9114798568024938 2316 50-51 -0.5555232948721667 2316 52-53 -2.836793953148373 2316 54-55 -1.2092934551932046 2316 56-57 -0.08084652589193553 2316 58-59 0.002403366160965703 2316 60-61 -1.007939072910137 2316 62-63 -1.2259323530576545 2316 64-65 0.0876735635578676 2316 66-67 1.079996560480204 2316 68-69 3.530753514558029 2316 70-71 3.7747315118980183 2316 72-73 2.196808528741254 2316 74-75 1.3709321581029599 2316 76-77 -0.15931656108007886 2316 78-79 0.14250720542206352 2316 80-81 -0.3567322573849161 2316 82-83 -0.275487792532612 2316 84-85 0.18583184412096898 2316 86-87 -0.08392350010658234 2316 88-89 0.3122373561738243 2316 90-91 0.4766276035579189 2316 92-93 0.8715919093799016 2316 94-95 1.6380528366743583 2316 96-97 1.678824976448137 2316 98-99 0.5983053883164047 2316 100-101 0.6510242769169352 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 11.0 4 29.0 5 81.0 6 228.0 7 506.0 8 1038.0 9 1778.0 10 2736.0 11 4205.0 12 5364.0 13 6730.0 14 8156.0 15 10051.0 16 12163.0 17 13805.0 18 12583.0 19 10615.0 20 10382.0 21 10644.0 22 10976.0 23 12617.0 24 15318.0 25 19415.0 26 24145.0 27 30112.0 28 36752.0 29 44149.0 30 51586.0 31 59864.0 32 69298.0 33 80234.0 34 93117.0 35 106489.0 36 111750.0 37 95754.0 38 41623.0 39 2673.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.047825073494195 21.127576476743968 15.293676794718843 21.530921655042995 2 15.772687307853447 22.431112448637986 39.24638228484995 22.54981795865862 3 19.37188785664278 27.01537093995579 30.867938925292826 22.744802278108605 4 12.815835844025077 18.389768177277123 37.45299827725903 31.341397701438765 5 12.570048014663007 39.04320729689149 34.33151651800771 14.055228170437795 6 29.436549640898974 37.61111285919936 18.08976345743886 14.862574042462812 7 26.63896383817248 34.42718862833634 21.461497919337962 17.47234961415322 8 25.682409949419068 35.40340850986839 20.050856257325584 18.863325283386956 9 26.18192616599671 17.190240947743522 21.313216333786965 35.3146165524728 10-11 24.238926079466346 27.87251323521314 27.35156108650677 20.536999598813747 12-13 25.706648285518753 25.509595037876704 28.11430661642661 20.669450060177937 14-15 22.707289396883333 26.615167986910315 27.31685060925246 23.360692006953894 16-17 20.66424183783539 29.82303469393173 28.17245980497139 21.34026366326148 18-19 20.447673429573566 28.029566289631248 31.24736348895591 20.275396791839277 20-21 21.743606585747663 26.864188620469935 31.1308240837614 20.261380710021005 22-23 21.1650822431818 27.11591332803663 30.85387774045609 20.865126688325482 24-25 20.928598680018563 27.40357763740629 31.171581853795143 20.49624182878 26-27 20.947984642743183 27.290842431772155 31.325440999981808 20.43573192550285 28-29 20.510840975262425 27.42184284532021 31.57216146323785 20.495154716179513 30-31 20.710795521033383 27.413364328553264 31.294306103363944 20.581534047049406 32-33 20.624751078047172 27.505568476278047 31.118218874307317 20.75146157136746 34-35 21.12263442150272 27.329340057194777 31.004861949736252 20.543163571566247 36-37 21.174990058389735 27.14089131949776 31.072256804589284 20.61186181752322 38-39 20.953437952953163 27.487775647761474 31.127937966487924 20.43084843279744 40-41 20.962455076585776 27.339229431463153 31.225544704857906 20.472770787093168 42-43 20.717030293872863 27.565303029781024 30.76572118179129 20.95194549455482 44-45 20.85725380147812 27.76219580915859 30.38465835296596 20.995892036397336 46-47 21.04884456418065 27.840653505089392 29.91873511841778 21.191766812312178 48-49 21.16536094976263 27.889532314155584 29.810653823097756 21.134452912984035 50-51 21.08821741708931 28.199256344571832 29.63410282721946 21.078423411119395 52-53 21.238806983635243 28.372814169603416 29.406768902530832 20.98160994423051 54-55 20.927091779301442 28.161580061873686 29.558953185392774 21.352374973432102 56-57 21.164710268459213 28.455240566645585 29.192172964422834 21.18787620047237 58-59 21.28256243017419 28.021490613837706 29.356737108778773 21.33920984720933 60-61 21.201639121789462 28.141699266191093 29.617085887700295 21.039575724319146 62-63 21.32947740995621 27.96634294151167 29.593539984473388 21.110639664058738 64-65 21.117565644075256 28.109603050319954 29.56819092524897 21.204640380355823 66-67 20.73532236647505 28.227059693215693 29.698909322191668 21.33870861811759 68-69 20.825032797240173 27.937051114114563 30.03741314804917 21.200502940596092 70-71 20.50193943099437 27.885275808129332 30.2395901984294 21.373194562446898 72-73 21.07160977649403 28.091025591085995 29.211637161879977 21.625727470540003 74-75 20.689313867232894 28.419633395445214 29.27409855698207 21.616954180339818 76-77 21.01571066801072 28.274088461633024 29.103287192978144 21.606913677378113 78-79 20.825861610332293 28.24062988161447 29.154298475891434 21.779210032161803 80-81 20.793250911779833 28.649995525911855 28.869225845086422 21.687527717221897 82-83 20.880367832728933 28.60128576260787 28.776509758265618 21.741836646397584 84-85 20.82107486450131 28.383829047457976 28.694650063324495 22.10044602471622 86-87 20.82834307785192 28.39096463850884 28.871700777398225 21.908991506241016 88-89 21.016158674188304 28.207324256104123 28.688800238353473 22.0877168313541 90-91 20.702307986551904 28.336825812947968 28.972896325963887 21.987969874536233 92-93 20.77062442998424 28.48925850629445 28.735677393820353 22.004439669900954 94-95 20.350704972878837 28.296735653527183 29.03455355167263 22.318005821921354 96-97 20.269196687750178 28.109374029729818 29.02496409692303 22.596465185596976 98-99 20.624600785864857 28.102019832982823 28.51919399434425 22.75418538680807 100-101 20.489246114337384 28.073534422611996 28.73858737953106 22.69863208351956 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2296.0 1 1576.5 2 1112.0 3 1357.5 4 1174.5 5 1084.5 6 1424.0 7 2287.0 8 3073.5 9 3338.0 10 3109.0 11 2570.0 12 2263.0 13 2164.0 14 2180.0 15 2163.5 16 2140.0 17 2242.0 18 2369.0 19 2478.5 20 2683.0 21 3025.0 22 3489.5 23 4043.0 24 4758.0 25 5679.5 26 7036.0 27 8656.5 28 10054.0 29 11738.5 30 13891.0 31 16113.5 32 18570.5 33 21392.5 34 24245.0 35 26774.0 36 29497.5 37 32070.0 38 32942.0 39 32723.5 40 32564.0 41 32780.5 42 33098.0 43 34030.0 44 35001.0 45 35169.5 46 35443.5 47 37068.0 48 40961.5 49 40454.5 50 35993.0 51 32948.0 52 30199.0 53 28649.5 54 26983.5 55 24760.5 56 22454.0 57 19773.5 58 17384.5 59 15178.5 60 12983.5 61 10624.0 62 8319.0 63 6379.5 64 4770.0 65 3478.5 66 2513.0 67 1826.5 68 1303.5 69 900.0 70 615.0 71 445.0 72 359.5 73 290.0 74 225.0 75 169.5 76 115.5 77 60.0 78 29.0 79 20.0 80 13.5 81 8.0 82 3.5 83 1.0 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02330420144269723 2 0.017896053428569182 3 0.0 4 0.0 5 2.949898916797118E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 4.916498194661862E-4 18-19 0.0014749494583985588 20-21 0.0 22-23 0.0 24-25 0.0 26-27 5.40814801412805E-4 28-29 0.016765258843796954 30-31 0.005211488086341575 32-33 0.009734666425430489 34-35 0.010619636100469624 36-37 0.1034431220156856 38-39 0.1063438559505361 40-41 0.12070003067894874 42-43 0.10673717580610903 44-45 0.101329027791981 46-47 0.1029514721962194 48-49 0.10491807147408416 50-51 0.10388560685320516 52-53 0.08230217977863959 54-55 0.07079757400313083 56-57 0.03982363537676109 58-59 0.016519433934063858 60-61 0.00963633646153725 62-63 0.0020157642598113636 64-65 0.004080693501569346 66-67 0.437420844379066 68-69 0.8372796425509152 70-71 0.9313814179967433 72-73 0.28904092886417093 74-75 0.15850790179589846 76-77 0.015634464259024726 78-79 0.009292181587910921 80-81 0.0016716093861850333 82-83 0.0 84-85 0.0 86-87 1.966599277864745E-4 88-89 6.883097472526608E-4 90-91 0.0036382086640497787 92-93 0.0016224444042384147 94-95 0.2330911794089189 96-97 0.24061342164675156 98-99 0.014012019854786308 100-101 0.0071289223822597015 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1016984.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.46183878219894 #Duplication Level Percentage of deduplicated Percentage of total 1 83.9748218072479 54.971462469099464 2 10.104855138053793 13.229647959295043 3 2.624143297149528 5.153437363781712 4 1.049690210289042 2.7485860526877506 5 0.5498141938968881 1.7995924060521375 6 0.33549100555625666 1.317711487116089 7 0.22026372319270873 1.0093207836045595 8 0.1686927499550557 0.8834350081026924 9 0.12197830256706907 0.7186431579814584 >10 0.7632231860977887 9.340161498134382 >50 0.05913743297319993 2.6187671108957664 >100 0.025160686527591406 2.754467576600287 >500 0.0010609925251509284 0.4951115184806223 >1k 0.0013641332466226219 1.7188009688656956 >5k 3.031407214716938E-4 1.2408546393023374 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6345 0.6239036209025904 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6161 0.6058109075462347 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4247 0.41760735665457865 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3045 0.2994147400549074 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1665 0.16371938988224002 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1564 0.15378806352902308 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1552 0.15260810396230423 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1475 0.14503669674252495 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1391 0.13677697977549302 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1354 0.13313877111144323 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1030 0.10127986281003437 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 9.832996389323725E-5 0.0 5 0.0 0.0 0.0 9.832996389323725E-5 0.0 6 0.0 0.0 0.0 9.832996389323725E-5 0.0 7 0.0 0.0 0.0 9.832996389323725E-5 0.0 8 0.0 0.0 0.0 9.832996389323725E-5 0.0 9 0.0 0.0 0.0 9.832996389323725E-5 0.0 10-11 0.0 0.0 0.0 9.832996389323725E-5 0.0 12-13 0.0 0.0 0.0 1.966599277864745E-4 0.0 14-15 0.0 0.0 0.0 2.949898916797118E-4 0.0 16-17 0.0 0.0 0.0 4.4248483751956763E-4 9.832996389323725E-5 18-19 0.0 0.0 0.0 5.899797833594236E-4 9.832996389323725E-5 20-21 0.0 0.0 0.0 8.358046930925166E-4 9.832996389323725E-5 22-23 0.0 0.0 0.0 0.002261589169544457 9.832996389323725E-5 24-25 0.0 0.0 0.0 0.003392383754316685 9.832996389323725E-5 26-27 0.0 0.0 0.0 0.0042281884474092025 9.832996389323725E-5 28-29 0.0 0.0 0.0 0.005899797833594235 9.832996389323725E-5 30-31 0.0 0.0 0.0 0.01007882129905682 9.832996389323725E-5 32-33 0.0 0.0 0.0 0.018436868229981987 9.832996389323725E-5 34-35 0.0 0.0 0.0 0.03515296209183232 9.832996389323725E-5 36-37 0.0 0.0 0.0 0.05958795811930178 9.832996389323725E-5 38-39 0.0 0.0 0.0 0.09641252959731914 9.832996389323725E-5 40-41 0.0 0.0 0.0 0.13953021876450367 9.832996389323725E-5 42-43 0.0 0.0 0.0 0.18043548374409035 9.832996389323725E-5 44-45 0.0 0.0 0.0 0.2319112198422001 9.832996389323725E-5 46-47 0.0 0.0 0.0 0.29995555485632025 9.832996389323725E-5 48-49 0.0 0.0 0.0 0.37016314907609166 9.832996389323725E-5 50-51 0.0 0.0 0.0 0.44715551080449645 9.832996389323725E-5 52-53 0.0 0.0 0.0 0.5220337783091966 9.832996389323725E-5 54-55 0.0 0.0 0.0 0.6050242678350888 9.832996389323725E-5 56-57 0.0 0.0 0.0 0.7026167569991268 9.832996389323725E-5 58-59 0.0 0.0 0.0 0.7978001620477806 9.832996389323725E-5 60-61 0.0 0.0 0.0 0.898293385146669 9.832996389323725E-5 62-63 1.966599277864745E-4 0.0 0.0 0.9879703122173014 9.832996389323725E-5 64-65 1.966599277864745E-4 0.0 0.0 1.0923967338719192 9.832996389323725E-5 66-67 1.966599277864745E-4 0.0 0.0 1.1976589602196297 9.832996389323725E-5 68-69 1.966599277864745E-4 0.0 9.832996389323725E-5 1.3294702768185143 9.832996389323725E-5 70-71 1.966599277864745E-4 0.0 9.832996389323725E-5 1.5058250670610354 9.832996389323725E-5 72-73 1.966599277864745E-4 0.0 9.832996389323725E-5 1.6993384360029262 9.832996389323725E-5 74-75 1.966599277864745E-4 0.0 9.832996389323725E-5 1.9098628886983473 9.832996389323725E-5 76-77 1.966599277864745E-4 0.0 9.832996389323725E-5 2.141429953666921 9.832996389323725E-5 78-79 1.966599277864745E-4 0.0 9.832996389323725E-5 2.40505258686469 9.832996389323725E-5 80-81 1.966599277864745E-4 0.0 9.832996389323725E-5 2.690504472046758 9.832996389323725E-5 82-83 1.966599277864745E-4 0.0 9.832996389323725E-5 2.992524956144836 9.832996389323725E-5 84-85 1.966599277864745E-4 0.0 9.832996389323725E-5 3.285548248546683 9.832996389323725E-5 86-87 1.966599277864745E-4 0.0 9.832996389323725E-5 3.594451830117288 9.832996389323725E-5 88-89 1.966599277864745E-4 0.0 9.832996389323725E-5 3.922775579556807 9.832996389323725E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 780 0.0 25.511663 2 GGTATCA 7390 0.0 24.046747 1 GTACATG 6630 0.0 23.229473 1 TACATGG 6695 0.0 22.362513 2 ACATGGG 6640 0.0 21.544556 3 GTATCAA 9670 0.0 21.464321 1 ATGGGAG 1585 0.0 20.322557 5 GAGTACT 5445 0.0 19.922155 12-13 CATGGGA 4690 0.0 19.796204 4 GTAATAC 200 1.3153327E-5 18.947794 3 AGTACTT 5695 0.0 18.798075 12-13 GATAGTC 205 1.6272083E-5 18.485653 7 GTACCTG 1085 0.0 18.34379 1 GTATAGG 390 1.6370905E-11 18.226202 1 GTACTTT 5960 0.0 18.160954 14-15 TATCAAC 11485 0.0 18.068712 2 ATCAACG 11460 0.0 18.063234 3 TCAACGC 11490 0.0 18.016071 4 CAACGCA 11560 0.0 17.825022 5 GTCGTGA 135 0.009549764 17.544254 6 >>END_MODULE