FastQCFastQC Report
Fri 27 May 2016
SRR939430_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939430_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1003558
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45780.4561769225097104No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35320.3519477698349273No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25500.25409592669282693No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18750.18683524021531392No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11120.11080575313036217No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA10730.10691958013388364No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG7750.028.3651392
GTACATG58200.026.3939591
TACATGG59200.025.0031682
GGTATCA62500.024.5780561
GTATCAA82600.023.9454081
ACATGGG60850.023.7806473
GTACCTG9200.023.4631731
GTATAGG3900.023.169361
GAGTACT43750.022.40523312-13
CATGGGA45200.021.9336384
GTACTTT50050.020.28413214-15
CATGGGG18600.019.7316554
ATGGGAT19100.019.5486725
TATCAAC98400.019.5106412
AGTACTT46200.019.18897412-13
ATCAACG100550.019.1725583
TCAACGC102400.018.8978234
TGGGATA8000.018.7719446
TACGAGA1300.00741405418.3321084
CAACGCA103050.018.3046515
Produced by FastQC (version 0.11.5)