FastQCFastQC Report
Fri 27 May 2016
SRR939430_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939430_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1003558
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63360.6313536437355888No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59610.593986595692526No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42050.419009165389544No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29770.2966445387311944No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16560.16501288415816526No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA14680.14627953740590977No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA14470.14418698271549826No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14060.1401015187961234No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT13320.132727754648959No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13080.130336263574203No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10430.10393021629043861No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG7450.032.4770772
GTACCTG9750.025.795421
GGTATCA72000.023.9246441
GTATCAA96500.023.8006821
GTACATG65850.023.7809661
TGTAGCG1000.001665297523.7210042
TACATGG66700.022.831912
ACATGGG65450.022.6133383
TGGACCG1100.002908495721.550585
GAGTACT57200.021.13193112-13
GTGTAGC6400.020.7610551
ATGGGAT20150.020.7056665
AGTACTT58650.020.6903112-13
CATGGGA46750.020.602544
ATCAACG114000.020.0984253
TATCAAC115150.019.9408862
CATGGGG17700.019.8364894
TCAACGC115300.019.7934784
ACCTGGG11750.019.7823543
CAACGCA116950.019.6212525
Produced by FastQC (version 0.11.5)