##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939430_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1003558 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.867522355459275 31.0 31.0 31.0 28.0 34.0 2 31.08972077348793 31.0 31.0 34.0 28.0 34.0 3 31.087458821513057 31.0 31.0 34.0 28.0 34.0 4 35.007858041089804 35.0 35.0 37.0 32.0 37.0 5 34.5868151118321 35.0 35.0 37.0 30.0 37.0 6 34.662737978273306 35.0 35.0 37.0 31.0 37.0 7 34.5011489121705 35.0 35.0 37.0 30.0 37.0 8 34.41636557129733 35.0 35.0 37.0 30.0 37.0 9 35.90302404046403 37.0 35.0 39.0 30.0 39.0 10-11 35.6879906293408 37.0 35.0 39.0 30.0 39.0 12-13 35.668080469688846 37.0 35.0 39.0 30.0 39.0 14-15 36.457944134768496 38.0 35.0 40.0 30.0 41.0 16-17 36.38256134672834 38.0 35.0 40.0 30.0 41.0 18-19 36.420665272958814 38.0 35.0 40.0 30.0 41.0 20-21 36.31289222944763 38.0 34.5 40.0 29.5 41.0 22-23 36.145014040045524 38.0 34.0 40.0 29.0 41.0 24-25 35.977920558652315 38.0 34.0 40.0 29.0 41.0 26-27 35.502550923813075 38.0 34.0 40.0 27.0 41.0 28-29 35.28404785772222 38.0 33.5 40.0 27.0 41.0 30-31 34.96992450859841 37.0 33.0 40.0 27.0 41.0 32-33 34.71478579215153 37.0 33.0 40.0 25.5 41.0 34-35 34.914205257693126 37.0 33.0 40.0 26.0 41.0 36-37 34.73474726921613 37.0 33.0 40.0 25.0 41.0 38-39 34.533786786613234 37.0 33.0 40.0 25.0 41.0 40-41 34.377477933512566 37.0 33.0 40.0 24.5 41.0 42-43 34.20218811468794 37.0 33.0 40.0 24.0 41.0 44-45 33.80867473529183 37.0 32.5 40.0 23.5 41.0 46-47 33.373281364903676 37.0 32.0 40.0 22.0 41.0 48-49 32.959694407298834 37.0 31.0 39.5 19.5 41.0 50-51 32.70893261774606 36.0 31.0 39.0 19.0 40.0 52-53 32.60812977426317 36.0 31.0 39.0 19.0 40.0 54-55 32.278127422630284 36.0 30.5 39.0 16.5 40.0 56-57 31.811407013844743 35.5 30.0 39.0 14.0 40.0 58-59 31.37410742577908 35.0 29.0 39.0 12.5 40.0 60-61 31.023049489914882 35.0 28.5 38.0 9.0 40.0 62-63 30.8570032823215 35.0 28.5 38.0 8.0 40.0 64-65 30.405638239145123 34.0 28.0 38.0 7.5 40.0 66-67 29.528431839515004 34.0 27.0 37.5 4.5 39.5 68-69 26.0856039212482 31.0 17.0 36.0 2.0 39.0 70-71 24.760071166788563 29.5 12.5 35.0 2.0 38.0 72-73 25.78419284186863 30.5 19.0 35.0 2.0 38.0 74-75 26.541131155349266 31.0 21.5 35.0 2.0 37.5 76-77 26.211426743646108 30.5 22.5 34.0 2.0 36.5 78-79 27.338586309909342 32.0 25.5 35.0 2.0 37.0 80-81 27.355168809376238 32.0 26.0 35.0 2.0 36.5 82-83 27.01160172107641 32.0 25.5 35.0 2.0 36.0 84-85 26.63808618933833 32.0 25.0 35.0 2.0 36.0 86-87 26.17831306212496 32.0 24.0 34.0 2.0 35.0 88-89 25.672080736738685 31.0 22.0 34.0 2.0 35.0 90-91 25.04657279399895 31.0 20.0 34.0 2.0 35.0 92-93 24.483968041707605 31.0 18.5 34.0 2.0 35.0 94-95 23.64185876650876 30.5 8.5 34.0 2.0 35.0 96-97 22.964204859111284 30.0 2.0 34.0 2.0 35.0 98-99 22.11164725905229 30.0 2.0 34.0 2.0 35.0 100-101 20.718629615826888 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.16503706920799033 1101 2 0.046479059559462854 1101 3 0.23124852365610238 1101 4 0.12314002289640769 1101 5 0.48078076140194526 1101 6 0.26914059761292464 1101 7 0.45391950436238915 1101 8 0.6871025555206245 1101 9 0.6422636003192892 1101 10-11 0.57318769521072 1101 12-13 0.5606131562777392 1101 14-15 0.5949306948734048 1101 16-17 0.48754383933388823 1101 18-19 0.42662988113365685 1101 20-21 0.5442442964957337 1101 22-23 0.6697811396135336 1101 24-25 0.7493240515347068 1101 26-27 0.7316326249421508 1101 28-29 0.38228500865339043 1101 30-31 0.2894815349445423 1101 32-33 0.6535958268172948 1101 34-35 0.5116957306239769 1101 36-37 0.7995819166692826 1101 38-39 0.8558052882613936 1101 40-41 0.6675793267992631 1101 42-43 0.4304144049350711 1101 44-45 0.8437250420462306 1101 46-47 0.5785564380863022 1101 48-49 0.5684578355267504 1101 50-51 0.1377429377185635 1101 52-53 -0.20182332147340531 1101 54-55 -0.13782587197937346 1101 56-57 -0.059747114447677774 1101 58-59 0.13767518870292506 1101 60-61 0.31064456503839466 1101 62-63 -0.2651247070913243 1101 64-65 -0.5041228593265181 1101 66-67 -0.5567379007635687 1101 68-69 -7.24217081261925 1101 70-71 -7.823594715537702 1101 72-73 -5.2399496838740305 1101 74-75 -3.4793410452659543 1101 76-77 -0.7188433325419297 1101 78-79 -0.1194477215157157 1101 80-81 -0.22964696970901954 1101 82-83 -0.597428045675116 1101 84-85 -0.5730261867068087 1101 86-87 -0.7456854687692172 1101 88-89 -0.7331554163311367 1101 90-91 -0.7726491491525529 1101 92-93 -0.843421751299001 1101 94-95 -0.602946202861073 1101 96-97 -0.582906771085753 1101 98-99 -1.0483206422768738 1101 100-101 -0.8549134358624713 1102 1 0.33041293742361333 1102 2 0.26764058903741983 1102 3 0.38286060326006677 1102 4 0.3576279825364992 1102 5 0.4730572475932391 1102 6 0.4496916219773368 1102 7 0.23292109776560466 1102 8 0.4465435877811288 1102 9 0.4564642745605312 1102 10-11 0.5783863505107973 1102 12-13 0.5990534178417306 1102 14-15 0.8409932867777812 1102 16-17 0.5761668458067888 1102 18-19 0.4487460340814451 1102 20-21 0.32060991870313416 1102 22-23 0.5352083087932371 1102 24-25 0.3998072735189453 1102 26-27 0.4242559245634254 1102 28-29 0.5018351677572994 1102 30-31 0.21444382755389313 1102 32-33 0.7198686344694138 1102 34-35 0.4325693127551702 1102 36-37 0.500520937080914 1102 38-39 0.657298926469025 1102 40-41 0.6209037238356316 1102 42-43 0.38206738346432445 1102 44-45 0.4055497960616563 1102 46-47 0.6776583165999384 1102 48-49 0.559109003155605 1102 50-51 0.45127338968715236 1102 52-53 0.19418111357768453 1102 54-55 -0.13782705402923767 1102 56-57 0.1604983263859978 1102 58-59 0.3329498261682282 1102 60-61 0.4164415739794194 1102 62-63 0.023207987935084162 1102 64-65 0.0437324294009791 1102 66-67 -0.1863142068104615 1102 68-69 -7.058596104703771 1102 70-71 -7.8319139824776585 1102 72-73 -1.6447674827080547 1102 74-75 -0.15024417864373163 1102 76-77 0.2693538995701097 1102 78-79 0.18245845209431266 1102 80-81 0.06464680277948176 1102 82-83 -0.1312393999732855 1102 84-85 -0.23703678732998767 1102 86-87 -0.09895880125147016 1102 88-89 -0.3171802488346671 1102 90-91 -0.6055132086900379 1102 92-93 -0.5368382063787429 1102 94-95 -0.1837641340846865 1102 96-97 -0.459717080451842 1102 98-99 -0.6192459981920884 1102 100-101 -0.6060848472772946 1103 1 0.38365423635456963 1103 2 0.18885873870384629 1103 3 0.358716643771281 1103 4 0.32074388961792266 1103 5 0.5128149037302094 1103 6 0.4762570106116897 1103 7 0.4299410318545043 1103 8 0.7096755707406999 1103 9 0.4647132095336062 1103 10-11 0.6453373592721476 1103 12-13 0.5835803851273198 1103 14-15 0.777820109380464 1103 16-17 0.643780984541614 1103 18-19 0.6516487348289743 1103 20-21 0.6338936177627019 1103 22-23 0.6204958660635569 1103 24-25 0.8007346507394217 1103 26-27 0.7217601444814363 1103 28-29 0.7378742723308918 1103 30-31 0.792241030351093 1103 32-33 0.9245748477963147 1103 34-35 0.7067886279227622 1103 36-37 0.8033955050416921 1103 38-39 0.8167867088016294 1103 40-41 1.0257395489300691 1103 42-43 0.7075637262020464 1103 44-45 1.031062214677526 1103 46-47 1.2764345403761652 1103 48-49 1.3387941168924442 1103 50-51 1.1022208617553133 1103 52-53 0.7029826568619839 1103 54-55 0.7251763219051739 1103 56-57 0.9947057855934567 1103 58-59 1.1230892844111402 1103 60-61 1.2308505845091204 1103 62-63 0.6642939135037658 1103 64-65 0.6282815010190177 1103 66-67 0.37298066498441074 1103 68-69 -6.485109246734158 1103 70-71 -7.5339676759106595 1103 72-73 -0.6070996907299175 1103 74-75 1.0633540047820382 1103 76-77 1.1654635370590576 1103 78-79 1.1032144202640737 1103 80-81 0.8463162827248247 1103 82-83 0.687605548182006 1103 84-85 0.48117818735050477 1103 86-87 0.40950615333417417 1103 88-89 0.23151961452036574 1103 90-91 0.1567094824119586 1103 92-93 0.28390068117048983 1103 94-95 0.36384558378365384 1103 96-97 0.5300697132142602 1103 98-99 0.6177375642225122 1103 100-101 0.607679118641844 1104 1 0.22913038941499053 1104 2 0.21297086253507302 1104 3 0.10261740593047008 1104 4 0.21640861814782397 1104 5 0.4635200974129319 1104 6 0.32677166449713013 1104 7 0.38917140821970975 1104 8 0.48979853766142156 1104 9 0.5799505003326928 1104 10-11 0.36074063577093085 1104 12-13 0.27415946896482524 1104 14-15 0.8150293888650211 1104 16-17 0.599676914454399 1104 18-19 0.621383354142246 1104 20-21 0.34117439877943667 1104 22-23 0.4908158550633246 1104 24-25 0.24018915690741238 1104 26-27 0.37904387198987877 1104 28-29 0.36100325009470424 1104 30-31 0.5644870427656485 1104 32-33 0.5814657097433624 1104 34-35 0.3140851235619522 1104 36-37 0.6357646808097428 1104 38-39 0.6165971511682571 1104 40-41 0.8104148455435691 1104 42-43 0.7094101943406415 1104 44-45 0.8607549938085057 1104 46-47 0.618021324378681 1104 48-49 0.6584344484709774 1104 50-51 0.5237606983232368 1104 52-53 0.15065296408285178 1104 54-55 0.3311026988989667 1104 56-57 0.4156121264436159 1104 58-59 0.5432688239726993 1104 60-61 0.8670619854677142 1104 62-63 0.3475853411772185 1104 64-65 -0.08941512819446373 1104 66-67 -0.17104483199052467 1104 68-69 -6.882158826368002 1104 70-71 -4.535715927658913 1104 72-73 -0.09491103668733203 1104 74-75 0.802402877257876 1104 76-77 0.8011593019683048 1104 78-79 0.47605365967634583 1104 80-81 0.2335835098796899 1104 82-83 -0.10918474018131263 1104 84-85 -0.005596975688700212 1104 86-87 -9.745176802056221E-4 1104 88-89 0.2194881067023431 1104 90-91 0.15029104130250914 1104 92-93 0.2288061970029709 1104 94-95 0.20122379545961522 1104 96-97 0.1557817820443077 1104 98-99 -0.10177115739295317 1104 100-101 0.1818079392594285 1105 1 0.22002531208734766 1105 2 0.23922946726606398 1105 3 0.22532255558995828 1105 4 0.342528842253941 1105 5 0.4171074197248501 1105 6 0.36027724569575525 1105 7 0.5041859358866461 1105 8 0.5656059878413302 1105 9 0.6158811904888211 1105 10-11 0.4312726119627186 1105 12-13 0.2627265935288463 1105 14-15 0.3956380184217778 1105 16-17 0.4801458608001141 1105 18-19 0.5749829360887091 1105 20-21 0.386336210630823 1105 22-23 0.6245968183028054 1105 24-25 0.5752380728492312 1105 26-27 0.6937230220294168 1105 28-29 0.6768838443191854 1105 30-31 0.7150916726166301 1105 32-33 0.7410394116013066 1105 34-35 0.7643600458665176 1105 36-37 0.7007415101004568 1105 38-39 0.8640731989072776 1105 40-41 0.8111726442035945 1105 42-43 0.6226836240347069 1105 44-45 0.7333076642633998 1105 46-47 0.9491735921045787 1105 48-49 0.8885975935576198 1105 50-51 0.7527564963334967 1105 52-53 0.6875862624762306 1105 54-55 0.5724274750073945 1105 56-57 0.7783720306958735 1105 58-59 0.9110827625641882 1105 60-61 1.1520490668778152 1105 62-63 1.001337632196421 1105 64-65 0.8204187077838831 1105 66-67 0.9608148166483481 1105 68-69 -2.157730508451163 1105 70-71 -2.6620457148929546 1105 72-73 1.2039930286074814 1105 74-75 1.5433885978485478 1105 76-77 1.4954894508615695 1105 78-79 0.9510049276290466 1105 80-81 0.8023248132014409 1105 82-83 0.758812709814702 1105 84-85 0.7088910772974408 1105 86-87 0.9369078296720161 1105 88-89 0.7765773936206983 1105 90-91 0.7895735155419494 1105 92-93 0.7434553109378861 1105 94-95 0.759403040511323 1105 96-97 0.4870267632564165 1105 98-99 0.9032357539760199 1105 100-101 0.7663484634814033 1106 1 0.03227366625202421 1106 2 0.15380981901367008 1106 3 0.06619492781907255 1106 4 0.08243836106575486 1106 5 0.21243467857419773 1106 6 0.2781667600550719 1106 7 0.11327619444976023 1106 8 0.09970896783687522 1106 9 0.28820014860350085 1106 10-11 0.2788022688020817 1106 12-13 0.004725607617828587 1106 14-15 0.009970302236936845 1106 16-17 0.031178284551025115 1106 18-19 0.0019070527442508478 1106 20-21 -0.0042845157629685104 1106 22-23 0.0067430829722070484 1106 24-25 -0.05918373804667709 1106 26-27 0.5835510441647074 1106 28-29 0.33988554581077324 1106 30-31 0.3097180600478566 1106 32-33 0.5823769112037596 1106 34-35 0.18911913373981548 1106 36-37 0.5048799510889097 1106 38-39 0.6400678559056558 1106 40-41 0.3415235967208403 1106 42-43 0.5345761756360119 1106 44-45 0.8253925480213056 1106 46-47 0.5128773576485841 1106 48-49 0.4319371285892686 1106 50-51 0.3408599692845513 1106 52-53 0.33632644312056925 1106 54-55 0.03875702371457379 1106 56-57 0.19783303950546482 1106 58-59 0.44345602529357464 1106 60-61 0.560460716529569 1106 62-63 0.022246499034825007 1106 64-65 0.21537187701074245 1106 66-67 0.397208847116179 1106 68-69 -3.626661192546191 1106 70-71 -3.370245075640973 1106 72-73 0.5079768246829204 1106 74-75 0.924698825880224 1106 76-77 0.8890233852825844 1106 78-79 0.5067414985669352 1106 80-81 0.42506914421311137 1106 82-83 0.33218675173182177 1106 84-85 0.146701957302529 1106 86-87 0.26003102084334273 1106 88-89 0.06761434938191613 1106 90-91 0.34083214226332004 1106 92-93 0.04409973099958364 1106 94-95 0.3314170817923703 1106 96-97 0.39957526371756913 1106 98-99 0.4141622718019242 1106 100-101 0.2558512940001165 1107 1 0.06457232012780523 1107 2 -0.03585004833084682 1107 3 0.07120552443564776 1107 4 -0.09322897595661317 1107 5 0.20192883065750777 1107 6 0.16420462474314945 1107 7 -0.008711889040753817 1107 8 -0.1510024003133168 1107 9 0.2148682309281824 1107 10-11 -0.07945705476321763 1107 12-13 0.028691448622780058 1107 14-15 -0.017403527242898065 1107 16-17 -0.12449246670536951 1107 18-19 -0.0065798170277062695 1107 20-21 0.15580641944569606 1107 22-23 0.11268676476559136 1107 24-25 0.06085365197959902 1107 26-27 0.3984991436438321 1107 28-29 0.3692338018450201 1107 30-31 -0.004540530706236723 1107 32-33 -0.038857958951254545 1107 34-35 -0.1197249814947483 1107 36-37 0.0750599478412255 1107 38-39 0.07266088337358667 1107 40-41 -0.20572565485407068 1107 42-43 -0.14720638762099725 1107 44-45 0.05531372917973698 1107 46-47 0.24854611032895946 1107 48-49 0.35617152824617904 1107 50-51 0.5179521244573237 1107 52-53 0.08656564768732267 1107 54-55 0.054380891708625256 1107 56-57 0.4073021261145904 1107 58-59 0.5784569204890779 1107 60-61 0.6720353862440618 1107 62-63 0.4293772517068817 1107 64-65 0.29288581499659117 1107 66-67 0.5507402892969608 1107 68-69 -2.436799385750202 1107 70-71 -2.5087892795623503 1107 72-73 1.1734622496509637 1107 74-75 1.3442032623026066 1107 76-77 0.9870477096181602 1107 78-79 0.9081479964372043 1107 80-81 0.43866195933233953 1107 82-83 0.3744412287502783 1107 84-85 0.028626502730578096 1107 86-87 0.16077757225879452 1107 88-89 0.3305961415216849 1107 90-91 0.38273270477919397 1107 92-93 0.41356065675664766 1107 94-95 0.4982844388004999 1107 96-97 0.48753176137965326 1107 98-99 0.5107163936567716 1107 100-101 0.7777461106099501 1108 1 -0.02958067688034305 1108 2 -0.08218503233992536 1108 3 -0.1522779172232802 1108 4 0.09879361766765271 1108 5 0.01019252610782928 1108 6 -0.1499473405926608 1108 7 -0.01450988759641092 1108 8 0.16704412810925362 1108 9 0.118481663302056 1108 10-11 0.14361500754979772 1108 12-13 0.2602541743011102 1108 14-15 0.13948159604195354 1108 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2316 94-95 1.4000558584198508 2316 96-97 1.463088768918503 2316 98-99 1.3507976283010912 2316 100-101 1.3384141757657915 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 10.0 4 41.0 5 90.0 6 229.0 7 563.0 8 1138.0 9 1876.0 10 2996.0 11 4397.0 12 5801.0 13 7107.0 14 8612.0 15 10888.0 16 12597.0 17 14518.0 18 13123.0 19 11336.0 20 11494.0 21 11592.0 22 12272.0 23 13552.0 24 16137.0 25 20467.0 26 25135.0 27 31202.0 28 38097.0 29 45265.0 30 52805.0 31 61227.0 32 70683.0 33 81082.0 34 92613.0 35 101995.0 36 102999.0 37 83355.0 38 34327.0 39 1934.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.907237352224186 21.167595861180928 15.328520258566561 21.59664652802832 2 15.801149902978807 22.686615061556388 39.062317804958994 22.449917230505818 3 19.267921805901825 27.129382175747395 31.0052074925714 22.59748852577938 4 12.735753093511404 18.480788715763943 37.578983269907376 31.204474920817276 5 12.451796508024449 39.1029716269513 34.389542009530096 14.055689855494153 6 29.297160702221497 37.8610902409228 18.05007782310539 14.791671233750318 7 26.457663632794514 34.56093220322094 21.469511478160705 17.511892685823838 8 25.686407761185702 35.37214590487047 20.04298705206874 18.898459281875088 9 26.131524037474662 17.220529356549395 21.2793879377176 35.36855866825834 10-11 24.21456916156267 27.901156197245136 27.40567156504182 20.478603076150367 12-13 25.690841984220146 25.54291829670034 28.171266633318652 20.594973085760863 14-15 22.64203962302129 26.595024901400816 27.440367173596343 23.32256830198155 16-17 20.65794364212681 29.86559726411513 28.192189534961354 21.284269558796712 18-19 20.379600520150866 28.076986313654956 31.286028169856163 20.25738499633802 20-21 21.696603484801077 26.944381889238088 31.149121425966413 20.20989319999442 22-23 21.204116356063306 27.043393128943784 30.93644360188629 20.81604691310662 24-25 20.866873805447657 27.38038532596456 31.305592149187756 20.44714871940003 26-27 20.9299869110665 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29.64841759497571 21.072557186862102 52-53 21.162978509150186 28.324700768206903 29.491119009755877 21.021201712887034 54-55 20.97912868971478 28.103816711703157 29.61516854912716 21.3018860494549 56-57 21.18333222031403 28.416130340872957 29.17519289289 21.22534454592301 58-59 21.312182896691535 27.96095564677488 29.40406380613174 21.32279765040184 60-61 21.267465268780768 28.013045384784043 29.740836339718165 20.97865300671703 62-63 21.37529178178086 27.975078036834883 29.67078794159631 20.978842239787948 64-65 21.152944494298158 28.119171911271795 29.510514040228543 21.217369554201507 66-67 20.836976178784866 28.213388904192165 29.507796687306133 21.441838229716836 68-69 21.141277641894263 27.926011551409392 29.74510505638929 21.187605750307057 70-71 20.841312399481364 27.844221815441372 30.054161289333297 21.260304495743966 72-73 21.13404819972386 27.998905847365705 29.284035122899642 21.58301083001079 74-75 20.978727455903826 28.367911872953965 29.036352207089383 21.617008464052827 76-77 20.39638630365953 28.36460071311393 29.534319231809953 21.704693751416592 78-79 20.857372361852615 28.253602989028337 29.19599602552514 21.69302862359391 80-81 20.782219936952465 28.593159038333244 28.885029174557573 21.73959185015672 82-83 20.902885140928824 28.562382011253177 28.782104523144852 21.752628324673143 84-85 20.839673282135443 28.334265019326253 28.653629041499386 22.17243265703891 86-87 20.81667550212649 28.3234615338624 28.861062726450175 21.998800237560935 88-89 21.019187298746605 28.131307268314774 28.706313408987754 22.143192023950867 90-91 20.80843119386275 28.28622292818767 28.861303532645117 22.04404234530446 92-93 20.76698710826289 28.419120468036724 28.80646051712438 22.00743190657601 94-95 20.529153768828184 28.237241112458516 28.83664156242022 22.39696355629308 96-97 20.53491458821266 28.050095223190098 28.77285443785921 22.642135750738035 98-99 20.583351936600067 28.079206065727995 28.506649339052508 22.830792658619426 100-101 20.488861272047863 28.004506616664017 28.816335438979046 22.690296672309074 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2246.0 1 1556.5 2 1101.0 3 1341.5 4 1151.0 5 1005.0 6 1341.5 7 2233.5 8 3057.0 9 3389.0 10 3090.5 11 2405.5 12 2154.0 13 2113.5 14 2077.5 15 2104.0 16 2102.5 17 2166.0 18 2320.5 19 2513.0 20 2743.0 21 3061.5 22 3538.5 23 4100.5 24 4701.0 25 5567.5 26 6908.5 27 8520.5 28 9944.0 29 11575.5 30 13694.0 31 15905.0 32 18323.5 33 21183.0 34 23965.0 35 26387.0 36 29362.5 37 31760.5 38 32509.0 39 32494.0 40 32106.5 41 32180.5 42 32737.5 43 33481.5 44 34267.5 45 34828.5 46 35254.0 47 36538.0 48 40024.5 49 39754.5 50 35380.5 51 32092.5 52 29550.5 53 28029.0 54 26576.0 55 24465.0 56 21986.5 57 19461.5 58 17251.5 59 15330.5 60 13122.5 61 10722.5 62 8501.0 63 6312.5 64 4493.5 65 3346.0 66 2527.5 67 1900.5 68 1360.0 69 882.5 70 640.0 71 478.0 72 370.0 73 309.0 74 248.0 75 186.5 76 122.5 77 66.0 78 35.0 79 21.0 80 9.5 81 6.5 82 6.5 83 3.5 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.017936183060670133 2 0.016840082984740296 3 3.985818457926697E-4 4 0.08111140561880828 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.009565964299024073 12-13 0.0 14-15 0.0 16-17 6.97518230137172E-4 18-19 2.9893638434450227E-4 20-21 0.0 22-23 4.982273072408371E-5 24-25 0.004384400303719367 26-27 0.003836350265754446 28-29 0.001046277345205758 30-31 3.48759115068586E-4 32-33 6.476954994130882E-4 34-35 0.0035374138814099434 36-37 0.08594421049904441 38-39 0.09506177022155173 40-41 0.0786202690826041 42-43 0.08235697388691038 44-45 0.08150998746460095 46-47 0.07966654642780985 48-49 0.0746344506246774 50-51 0.0746344506246774 52-53 0.057096849409799935 54-55 0.04807893514874078 56-57 0.028000374666935043 58-59 0.023665797093939765 60-61 0.013751073679847103 62-63 0.002540959266928269 64-65 0.006626423186303134 66-67 0.15883486554837886 68-69 0.7376255283700593 70-71 0.8996490486847796 72-73 0.36843909370459904 74-75 0.054954471988664336 76-77 0.20277851404702069 78-79 0.015445046524465949 80-81 0.002939541112720939 82-83 0.0019430864982392646 84-85 9.964546144816742E-5 86-87 0.002590781997652353 88-89 3.48759115068586E-4 90-91 0.004633513957339785 92-93 0.004683336688063869 94-95 0.08031424192722295 96-97 0.03871226177261305 98-99 0.014946819217225113 100-101 0.014398769179260192 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1003558.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.11211652982661 #Duplication Level Percentage of deduplicated Percentage of total 1 84.25546525704212 55.70307137348326 2 10.03801978275532 13.27269467212449 3 2.5047062569503993 4.967742957974719 4 1.0042422393771067 2.6557031981549324 5 0.5277972190074848 1.7446895623570626 6 0.3324760220204179 1.3188416106712242 7 0.22118544210710556 1.0236126406300328 8 0.16710345771267224 0.8838050615069712 9 0.11906827864830449 0.7084670321702315 >10 0.7470043016905287 9.20145525173051 >50 0.05696581851683086 2.555369559788677 >100 0.02353636447210707 2.666849643186344 >500 7.59237406149858E-4 0.3724453556693357 >1k 0.0013666273310697444 1.6907613693215504 >5k 3.036949624599432E-4 1.2344907112306795 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6336 0.6313536437355888 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5961 0.593986595692526 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4205 0.419009165389544 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2977 0.2966445387311944 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1656 0.16501288415816526 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1468 0.14627953740590977 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1447 0.14418698271549826 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1406 0.1401015187961234 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1332 0.132727754648959 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1308 0.130336263574203 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1043 0.10393021629043861 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 9.964546144816742E-5 0.0 14-15 0.0 0.0 0.0 1.9929092289633485E-4 0.0 16-17 0.0 0.0 0.0 2.4911365362041856E-4 0.0 18-19 0.0 0.0 0.0 3.985818457926697E-4 0.0 20-21 0.0 0.0 0.0 6.476954994130882E-4 0.0 22-23 0.0 0.0 0.0 0.00134521372955026 0.0 24-25 0.0 0.0 0.0 0.002640604728376437 0.0 26-27 0.0 0.0 0.0 0.0035872366121340272 0.0 28-29 0.0 0.0 0.0 0.005629968571821459 0.0 30-31 0.0 0.0 0.0 0.011259937143642918 0.0 32-33 0.0 0.0 0.0 0.02082590144266699 0.0 34-35 0.0 0.0 0.0 0.03866243904188896 0.0 36-37 0.0 0.0 0.0 0.06601511820941092 0.0 38-39 0.0 0.0 0.0 0.10138925702351034 0.0 40-41 0.0 0.0 0.0 0.14119761887205323 0.0 42-43 0.0 0.0 0.0 0.18329782633390396 0.0 44-45 0.0 0.0 0.0 0.2354622254020196 0.0 46-47 0.0 0.0 0.0 0.2997335480360876 0.0 48-49 0.0 9.964546144816742E-5 0.0 0.3664461844756357 0.0 50-51 0.0 9.964546144816742E-5 0.0 0.43754322121890316 0.0 52-53 0.0 9.964546144816742E-5 0.0 0.510682989921858 0.0 54-55 0.0 9.964546144816742E-5 0.0 0.596926136805247 0.0 56-57 0.0 9.964546144816742E-5 0.0 0.6960235482154494 0.0 58-59 0.0 9.964546144816742E-5 0.0 0.7988576644299582 4.982273072408371E-5 60-61 0.0 9.964546144816742E-5 0.0 0.905328839987325 9.964546144816742E-5 62-63 0.0 9.964546144816742E-5 0.0 1.003429796783046 9.964546144816742E-5 64-65 0.0 9.964546144816742E-5 0.0 1.1149828908742694 9.964546144816742E-5 66-67 0.0 9.964546144816742E-5 0.0 1.2294257033474896 9.964546144816742E-5 68-69 0.0 9.964546144816742E-5 0.0 1.3600110805753132 9.964546144816742E-5 70-71 0.0 9.964546144816742E-5 0.0 1.5288603149992328 9.964546144816742E-5 72-73 0.0 9.964546144816742E-5 0.0 1.715296973368754 9.964546144816742E-5 74-75 0.0 9.964546144816742E-5 0.0 1.9145878962650889 9.964546144816742E-5 76-77 0.0 9.964546144816742E-5 0.0 2.135452061564952 9.964546144816742E-5 78-79 0.0 9.964546144816742E-5 0.0 2.3990143070953547 9.964546144816742E-5 80-81 0.0 9.964546144816742E-5 0.0 2.6903776363697967 9.964546144816742E-5 82-83 0.0 9.964546144816742E-5 0.0 2.987819338792576 9.964546144816742E-5 84-85 0.0 9.964546144816742E-5 0.0 3.28994437790342 9.964546144816742E-5 86-87 0.0 9.964546144816742E-5 0.0 3.6073649953465567 9.964546144816742E-5 88-89 0.0 9.964546144816742E-5 0.0 3.9505439645740457 9.964546144816742E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 745 0.0 32.477077 2 GTACCTG 975 0.0 25.79542 1 GGTATCA 7200 0.0 23.924644 1 GTATCAA 9650 0.0 23.800682 1 GTACATG 6585 0.0 23.780966 1 TGTAGCG 100 0.0016652975 23.721004 2 TACATGG 6670 0.0 22.83191 2 ACATGGG 6545 0.0 22.613338 3 TGGACCG 110 0.0029084957 21.55058 5 GAGTACT 5720 0.0 21.131931 12-13 GTGTAGC 640 0.0 20.761055 1 ATGGGAT 2015 0.0 20.705666 5 AGTACTT 5865 0.0 20.69031 12-13 CATGGGA 4675 0.0 20.60254 4 ATCAACG 11400 0.0 20.098425 3 TATCAAC 11515 0.0 19.940886 2 CATGGGG 1770 0.0 19.836489 4 TCAACGC 11530 0.0 19.793478 4 ACCTGGG 1175 0.0 19.782354 3 CAACGCA 11695 0.0 19.621252 5 >>END_MODULE