FastQCFastQC Report
Fri 27 May 2016
SRR939429_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939429_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2813241
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146580.521036057699998No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114390.4066128710622375No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75230.2674139897719392No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA48040.1707638982938184No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29930.10638974762560335No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA99350.042.6085241
GTATCAA167750.039.642081
TATCAAC215750.031.0090122
ATCAACG220500.030.4786343
TCAACGC224150.029.9146774
CAACGCA225200.029.61375
AACGCAG231600.028.7252626
GTACATG149100.025.471711
ACGCAGA257300.025.3985777
CGCAGAG257500.025.264788
TACATGG149600.024.5294322
GCAGAGT255300.023.9369479
GAGTACT143200.023.11559312-13
ACATGGG154100.023.0336073
AGTACTT151250.022.09117712-13
CATGGGA102200.021.5891194
GTACTTT155200.020.98527714-15
GTGTAGC17000.020.8979361
TAGCCCT17550.020.726284
AGAGTAC237350.020.30322310-11
Produced by FastQC (version 0.11.5)