FastQCFastQC Report
Fri 27 May 2016
SRR939429_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939429_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2813241
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT204570.7271684153614995No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182620.6491445276106811No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125510.4461402346972762No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA79560.2828054901801872No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49560.1761669192223489No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37570.13354703702953283No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA111400.045.6851921
GTATCAA182400.035.981671
ATCAACG236100.027.6655543
TATCAAC236550.027.6183242
TCAACGC240450.027.1256564
CAACGCA243900.026.6836995
AACGCAG249600.026.0743356
ACGCAGA282800.022.9295277
GTACATG167850.022.8675731
CGCAGAG284400.022.7505658
TACATGG166300.022.363172
GAGTACT164950.021.85300312-13
GCAGAGT293500.021.5126119
ACATGGG169750.021.374213
AGTACTT171200.020.73703612-13
GTACTTT175500.020.33690814-15
CATGGGA115500.019.971844
AGAGTAC273350.019.44217510-11
CAGAGTA289900.019.0593310-11
TGGTATC61000.018.7949662
Produced by FastQC (version 0.11.5)