##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939429_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2813241 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.973021507933378 31.0 31.0 31.0 30.0 34.0 2 31.227661263290276 31.0 31.0 34.0 30.0 34.0 3 31.201229827092668 31.0 31.0 34.0 28.0 34.0 4 35.148706065353096 35.0 35.0 37.0 33.0 37.0 5 34.77653674178643 35.0 35.0 37.0 32.0 37.0 6 34.74081068774414 35.0 35.0 37.0 32.0 37.0 7 34.624065268492814 35.0 35.0 37.0 30.0 37.0 8 34.63948022938668 35.0 35.0 37.0 30.0 37.0 9 35.977934702359306 37.0 35.0 39.0 30.0 39.0 10-11 35.84498857367712 37.0 35.0 39.0 30.0 39.0 12-13 35.80802444582601 37.0 35.0 39.0 30.0 39.0 14-15 36.628096739667875 38.0 35.5 40.0 30.0 41.0 16-17 36.642776960807836 38.0 35.5 40.0 30.5 41.0 18-19 36.640794727504684 38.0 35.0 40.0 30.0 41.0 20-21 36.55814983501236 38.0 35.0 40.0 30.0 41.0 22-23 36.410508733521226 38.0 35.0 40.0 30.0 41.0 24-25 36.1990959892878 38.0 34.0 40.0 29.5 41.0 26-27 35.81148095737265 38.0 34.0 40.0 28.0 41.0 28-29 35.53083774194958 38.0 34.0 40.0 27.0 41.0 30-31 35.27951480161138 38.0 34.0 40.0 27.0 41.0 32-33 35.02062372189229 37.0 33.0 40.0 27.0 41.0 34-35 34.94614538889487 37.5 33.0 40.0 26.0 41.0 36-37 34.90804982580589 38.0 33.0 40.0 25.5 41.0 38-39 34.85755237464547 38.0 33.0 40.0 25.5 41.0 40-41 34.61321888170974 38.0 33.0 40.0 25.0 41.0 42-43 34.28608622581571 37.0 33.0 40.0 24.0 41.0 44-45 34.065707488267094 37.0 33.0 40.0 24.0 41.0 46-47 33.68183493699971 37.0 32.5 40.0 23.0 41.0 48-49 33.32604707524169 37.0 32.0 40.0 21.0 41.0 50-51 33.050184111492754 37.0 31.5 40.0 19.5 41.0 52-53 32.67772259824167 36.5 31.0 39.5 18.0 41.0 54-55 32.46588632115059 36.0 31.0 39.0 17.5 40.5 56-57 32.08185416748867 36.0 30.5 39.0 14.5 40.0 58-59 31.664487507469143 35.0 30.0 39.0 12.5 40.0 60-61 31.394437056761223 35.0 29.0 39.0 10.5 40.0 62-63 31.00833966944176 35.0 29.0 38.0 8.0 40.0 64-65 30.58935974557459 34.5 28.5 38.0 7.5 40.0 66-67 28.903201325446346 33.5 25.0 37.5 4.5 39.5 68-69 25.25052954937028 30.0 15.5 35.0 2.0 39.0 70-71 24.644295138596373 29.5 13.0 35.0 2.0 38.0 72-73 26.47169830099874 31.0 21.0 35.0 2.0 38.0 74-75 27.08166683906569 31.5 23.0 35.0 2.0 38.0 76-77 27.15536031218086 31.5 25.0 34.5 2.0 36.5 78-79 27.98187304251573 33.0 26.0 35.0 2.0 37.0 80-81 27.83372043134591 33.0 26.0 35.0 2.0 37.0 82-83 27.424349886838705 33.0 26.0 35.0 2.0 36.0 84-85 27.071038172698323 32.0 25.5 35.0 2.0 36.0 86-87 26.617061069421354 32.0 25.0 35.0 2.0 35.5 88-89 26.115444073223728 32.0 24.0 34.0 2.0 35.0 90-91 25.65295507921291 32.0 22.5 34.0 2.0 35.0 92-93 25.085667562786124 31.0 19.5 34.0 2.0 35.0 94-95 23.70803283472692 31.0 7.0 34.0 2.0 35.0 96-97 22.898813148251428 30.0 2.0 34.0 2.0 35.0 98-99 22.554795163300973 30.0 2.0 34.0 2.0 35.0 100-101 21.297010281024626 28.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.318184961833051 1101 2 0.24078318522160913 1101 3 0.2733943570855324 1101 4 0.270676716997869 1101 5 0.34596541989933627 1101 6 0.4445273051817509 1101 7 0.5153323421601996 1101 8 0.3640320028923796 1101 9 0.46090061659436543 1101 10-11 0.6482435056014353 1101 12-13 0.4962546691380325 1101 14-15 0.5506811830222205 1101 16-17 0.45952900656394036 1101 18-19 0.636601788922114 1101 20-21 0.6814663353547559 1101 22-23 0.6870364590484996 1101 24-25 0.5926497259837902 1101 26-27 0.7714552284007752 1101 28-29 0.5783692635917674 1101 30-31 0.7204548740160917 1101 32-33 0.5538264758657192 1101 34-35 0.5736745616607664 1101 36-37 0.589279672568054 1101 38-39 0.6176604922288718 1101 40-41 0.7135832778573317 1101 42-43 0.8255395831404471 1101 44-45 0.5838377879994567 1101 46-47 0.5236699646175609 1101 48-49 0.5966671613192176 1101 50-51 0.5293744659380764 1101 52-53 0.5108537347195465 1101 54-55 0.4414815052607679 1101 56-57 0.4193233874474167 1101 58-59 0.42084954648379025 1101 60-61 0.3103722973298133 1101 62-63 0.179671931589521 1101 64-65 -0.06297779677618465 1101 66-67 -3.245287593367589 1101 68-69 -8.130511640443256 1101 70-71 -11.493511977209259 1101 72-73 -11.259310837500838 1101 74-75 -9.771925323418003 1101 76-77 -4.777092491946679 1101 78-79 -1.8081293990612224 1101 80-81 -1.2524909593145601 1101 82-83 -0.9219100060194556 1101 84-85 -0.9095067991829282 1101 86-87 -0.8989762001583195 1101 88-89 -0.9015115900441586 1101 90-91 -0.841886409938791 1101 92-93 -1.5247669556714563 1101 94-95 -5.098091158079871 1101 96-97 -2.0004442034764587 1101 98-99 -1.2551098934605989 1101 100-101 -1.0105833818006182 1102 1 0.38062969525307366 1102 2 0.2412513256637432 1102 3 0.42506221228079966 1102 4 0.18782298991200008 1102 5 0.3944439634624004 1102 6 0.5154560354137274 1102 7 0.384672918526654 1102 8 0.4664410804543593 1102 9 0.6027165484707027 1102 10-11 0.5651454310177755 1102 12-13 0.6642875144755109 1102 14-15 0.5725837376595777 1102 16-17 0.5914080702830518 1102 18-19 0.6381398148460207 1102 20-21 0.7446508229591018 1102 22-23 0.6410863353017007 1102 24-25 0.7613852014651528 1102 26-27 0.7767801161896912 1102 28-29 0.7350113207250359 1102 30-31 0.5433004212286363 1102 32-33 0.5093342572323891 1102 34-35 0.7389625351495894 1102 36-37 0.847450317309864 1102 38-39 0.7425774350536614 1102 40-41 0.7238651688762587 1102 42-43 0.991984694093091 1102 44-45 0.7825793459507082 1102 46-47 0.772599659655107 1102 48-49 0.6488564310033453 1102 50-51 0.6081810433951844 1102 52-53 0.6870905735131245 1102 54-55 0.558535911905345 1102 56-57 0.5343928115004353 1102 58-59 0.48167571368878725 1102 60-61 0.3579602834030524 1102 62-63 0.4570680052503313 1102 64-65 0.4662149126495443 1102 66-67 -2.622298164280803 1102 68-69 -7.561361298566496 1102 70-71 -11.072700282087819 1102 72-73 -9.501817863802273 1102 74-75 -7.945674432021555 1102 76-77 -4.148570741873272 1102 78-79 -1.3730197048626422 1102 80-81 -0.7205716254498853 1102 82-83 -0.4826378050043125 1102 84-85 -0.4541391243643531 1102 86-87 -0.41676847332414013 1102 88-89 -0.3376645327011474 1102 90-91 -0.45276731937291004 1102 92-93 -0.8090473365599173 1102 94-95 -4.6640790350881325 1102 96-97 -1.2570176755180675 1102 98-99 -0.4650203343347954 1102 100-101 -0.3866658517028796 1103 1 0.3633673378386284 1103 2 0.2508710975268613 1103 3 0.23914250992175212 1103 4 0.09963050452829947 1103 5 0.3923146858958617 1103 6 0.43299272947317036 1103 7 0.3921339758061819 1103 8 0.3908255606048101 1103 9 0.5733101949811896 1103 10-11 0.6597517109147404 1103 12-13 0.4649933896134897 1103 14-15 0.7773372026417888 1103 16-17 0.6256412768736794 1103 18-19 0.5473198712669713 1103 20-21 0.7433821692937812 1103 22-23 0.6485960454288531 1103 24-25 0.8400409110199121 1103 26-27 0.7670795969534154 1103 28-29 0.7645652681472512 1103 30-31 0.7219559263596764 1103 32-33 0.7661988060692195 1103 34-35 0.8297399585929028 1103 36-37 0.7397116526056706 1103 38-39 0.7482503979711908 1103 40-41 0.7991070021482685 1103 42-43 0.8700894816968372 1103 44-45 0.5166629143713379 1103 46-47 0.5406028486652872 1103 48-49 0.6541626577501489 1103 50-51 0.6844762721453392 1103 52-53 0.6705328646942803 1103 54-55 0.5873081429859326 1103 56-57 0.368167086818314 1103 58-59 0.600835941007972 1103 60-61 0.43133000416024103 1103 62-63 0.5328910151853243 1103 64-65 0.5736375382679277 1103 66-67 -2.72409280020214 1103 68-69 -7.744299071983235 1103 70-71 -11.417185134289463 1103 72-73 -9.317688300042512 1103 74-75 -7.484401281034188 1103 76-77 -3.700130699353135 1103 78-79 -0.7896037843425852 1103 80-81 -0.20791984083072634 1103 82-83 0.07232723853794099 1103 84-85 0.02858972800082782 1103 86-87 0.09990841452900057 1103 88-89 0.1457802115647624 1103 90-91 0.06381614683584402 1103 92-93 0.03594366879598354 1103 94-95 1.0325463379851705 1103 96-97 1.014313122961017 1103 98-99 0.833533572202672 1103 100-101 0.6712864357986561 1104 1 0.3235880683067762 1104 2 0.19464185632786624 1104 3 0.31405603127892334 1104 4 0.05678113745651103 1104 5 0.34339852336071885 1104 6 0.4409579471166296 1104 7 0.4304745524432363 1104 8 0.29805962140989806 1104 9 0.5191397677168865 1104 10-11 0.5775758563075968 1104 12-13 0.5462828607858725 1104 14-15 0.702191249165665 1104 16-17 0.6636927858852175 1104 18-19 0.6748924825172296 1104 20-21 0.606717973445484 1104 22-23 0.5800016873522935 1104 24-25 0.7977415879878933 1104 26-27 0.88870985982674 1104 28-29 0.850783689699071 1104 30-31 0.8387792694917025 1104 32-33 0.6511316167247898 1104 34-35 0.8174369142305338 1104 36-37 0.8656984696720826 1104 38-39 0.8850940151042934 1104 40-41 0.8630383701842064 1104 42-43 1.063836227043062 1104 44-45 0.8529302690008222 1104 46-47 0.70642239918174 1104 48-49 0.673457997267171 1104 50-51 0.7325484901186385 1104 52-53 0.8880612368176486 1104 54-55 0.8041771267848503 1104 56-57 0.7555269177152688 1104 58-59 0.8087234396336243 1104 60-61 0.7705475400837685 1104 62-63 0.729989374296121 1104 64-65 0.5739491090680495 1104 66-67 -2.7505376550400484 1104 68-69 -7.355666679010749 1104 70-71 -8.738842793645166 1104 72-73 -8.137929316593976 1104 74-75 -6.940666436695743 1104 76-77 -3.5220528087674943 1104 78-79 -0.624573053161587 1104 80-81 0.16453246754190332 1104 82-83 0.3531642901810663 1104 84-85 0.26828720075839385 1104 86-87 0.39897847461398683 1104 88-89 0.3696691286455476 1104 90-91 0.3085890671498426 1104 92-93 0.5278875322859911 1104 94-95 1.3311876295284506 1104 96-97 1.5318971640116423 1104 98-99 1.097000740524006 1104 100-101 1.0501655904283886 1105 1 0.4919758490992301 1105 2 0.39035388976630614 1105 3 0.5192743710897325 1105 4 0.3302754299450825 1105 5 0.5036106301290673 1105 6 0.5901293191192707 1105 7 0.620220892058498 1105 8 0.5668711879812349 1105 9 0.8922533557494461 1105 10-11 0.9592934872625989 1105 12-13 0.9325484739461487 1105 14-15 1.1015560288092843 1105 16-17 1.0892606343856173 1105 18-19 0.9847827015292197 1105 20-21 1.0756485483519853 1105 22-23 1.1877095776507502 1105 24-25 1.16953966796639 1105 26-27 1.259734003700025 1105 28-29 1.190949079203122 1105 30-31 1.1424919017910824 1105 32-33 1.2865623382911124 1105 34-35 1.32545447063346 1105 36-37 1.267571285051801 1105 38-39 1.2336950942761575 1105 40-41 1.2241929190830447 1105 42-43 1.2987382093949904 1105 44-45 1.2757762028572444 1105 46-47 1.2988837787617982 1105 48-49 1.102364495519474 1105 50-51 1.1947753361991573 1105 52-53 1.3015415660689555 1105 54-55 1.4893744148003023 1105 56-57 1.4649893789115218 1105 58-59 1.4908640925142649 1105 60-61 1.4172499856363032 1105 62-63 1.4928506768797796 1105 64-65 1.5960484527653414 1105 66-67 2.1759431508556766 1105 68-69 -4.735451455721172 1105 70-71 -5.255014177869455 1105 72-73 -1.4615004527187345 1105 74-75 1.2811545588800186 1105 76-77 1.4588899694584114 1105 78-79 1.3872894349223053 1105 80-81 1.2723460751291071 1105 82-83 1.3610057020262936 1105 84-85 1.3535294033444956 1105 86-87 1.5012526184872748 1105 88-89 1.5279320347099343 1105 90-91 1.5202909932821775 1105 92-93 1.5654594872614247 1105 94-95 2.4632503693783683 1105 96-97 2.397116939866546 1105 98-99 2.2306568782375784 1105 100-101 2.0292450250547702 1106 1 0.08388840967584343 1106 2 0.06156472508811106 1106 3 0.08311197656069425 1106 4 0.07402691617338775 1106 5 0.30066718060343334 1106 6 0.3413511012812478 1106 7 0.2929645925004749 1106 8 0.2828023253866334 1106 9 0.42479171068946187 1106 10-11 0.4857967303152506 1106 12-13 0.38851685930772106 1106 14-15 0.3687082308871368 1106 16-17 0.22769844444358966 1106 18-19 0.24675345050629005 1106 20-21 0.41676864174861805 1106 22-23 0.49359739918855183 1106 24-25 0.45709086596890103 1106 26-27 0.5768846170510713 1106 28-29 0.6753086788029634 1106 30-31 0.7292276729488307 1106 32-33 0.7890863126910901 1106 34-35 0.8378125614553298 1106 36-37 0.8179170637534412 1106 38-39 0.7610336488832559 1106 40-41 0.8696776037566423 1106 42-43 0.9766491668950081 1106 44-45 1.018147034243107 1106 46-47 1.0110818181704317 1106 48-49 0.8509593947839491 1106 50-51 0.7842955817035744 1106 52-53 1.0212343473043504 1106 54-55 0.9575818902211566 1106 56-57 1.0340563042844089 1106 58-59 1.082107901604651 1106 60-61 1.1260857153846473 1106 62-63 1.1976313608317888 1106 64-65 1.1534419260706308 1106 66-67 1.7659069586340799 1106 68-69 -5.163701478270841 1106 70-71 -6.032735820633473 1106 72-73 -2.1500855531262673 1106 74-75 0.8111383948660134 1106 76-77 1.1599916233742604 1106 78-79 1.1641395841601252 1106 80-81 1.2938253540316325 1106 82-83 1.2294189431929716 1106 84-85 1.1851476804676508 1106 86-87 1.2267971930364858 1106 88-89 1.181464901880073 1106 90-91 0.9361187096975137 1106 92-93 1.0457248866365951 1106 94-95 1.9146982119898652 1106 96-97 2.0027568687735844 1106 98-99 1.579964449597032 1106 100-101 1.4520187063090262 1107 1 -0.021125034035900825 1107 2 -0.023275745438670725 1107 3 -0.054227184432370734 1107 4 0.01859019736944134 1107 5 0.14659008218386305 1107 6 0.21664093535691364 1107 7 0.0524659871460571 1107 8 0.1454426165526641 1107 9 0.22342652048425293 1107 10-11 0.10020371858379207 1107 12-13 0.16726985986615972 1107 14-15 0.08919527312661302 1107 16-17 0.09285621118325338 1107 18-19 0.2531939991357248 1107 20-21 0.1557036815749271 1107 22-23 0.15457632962024093 1107 24-25 0.3631460330624776 1107 26-27 0.30889446082282035 1107 28-29 0.39626576594103824 1107 30-31 0.2250317934063233 1107 32-33 0.18933499371788542 1107 34-35 0.3709412612401124 1107 36-37 0.39799773645428616 1107 38-39 0.3865310995950324 1107 40-41 0.38632818678338054 1107 42-43 0.4810516721879097 1107 44-45 0.4094658009304055 1107 46-47 0.3623058019440961 1107 48-49 0.3597152782983386 1107 50-51 0.4473049412972543 1107 52-53 0.43508533394491167 1107 54-55 0.5013221417309026 1107 56-57 0.5393066426979622 1107 58-59 0.4754998619959281 1107 60-61 0.31119818925916576 1107 62-63 0.3416598870759735 1107 64-65 0.3252047701922116 1107 66-67 1.2900234484120006 1107 68-69 -5.135603244571588 1107 70-71 -5.949007228198123 1107 72-73 -2.3031693146786907 1107 74-75 0.1981934845662039 1107 76-77 0.27413910388562357 1107 78-79 0.2363313462073684 1107 80-81 0.19556161529670035 1107 82-83 0.3289945624809043 1107 84-85 0.393162287583543 1107 86-87 0.42027201233543465 1107 88-89 0.3096041582484972 1107 90-91 0.3340391743248752 1107 92-93 0.47168188928862165 1107 94-95 1.3455735541844298 1107 96-97 1.420011026373647 1107 98-99 1.038462400341043 1107 100-101 1.0105972629994753 1108 1 0.09447786279234194 1108 2 0.05026965027107266 1108 3 0.12582390794031895 1108 4 -0.016559715712048728 1108 5 0.15631569121109123 1108 6 0.15872913117457443 1108 7 0.1372902973478034 1108 8 0.15611638867931532 1108 9 0.22981712269803012 1108 10-11 0.15836131515896312 1108 12-13 0.23780085681632812 1108 14-15 0.28044832978928014 1108 16-17 0.14165021687990986 1108 18-19 0.3304641688402725 1108 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1.4140495622838571 2312 84-85 1.3088779790999716 2312 86-87 1.39310165087959 2312 88-89 1.4343314532204872 2312 90-91 1.4468317091037726 2312 92-93 1.4457574861516242 2312 94-95 2.2267426874750598 2312 96-97 2.390507121596446 2312 98-99 2.025074781869865 2312 100-101 1.8727979197855298 2313 1 -0.216396882820014 2313 2 -0.27972874078144017 2313 3 -0.15541576435621707 2313 4 -0.07273589981043216 2313 5 -0.14042314617214657 2313 6 0.01133214729883747 2313 7 -3.479311649043808 2313 8 -0.9972760988404659 2313 9 -0.610604638969015 2313 10-11 0.023546191922285686 2313 12-13 -1.2999180904971652 2313 14-15 -0.3542504708925662 2313 16-17 -0.03391584998272634 2313 18-19 0.2554600377588869 2313 20-21 0.14325279488065235 2313 22-23 -1.1865184230307406 2313 24-25 -2.8051808150389803 2313 26-27 -1.9788004753867696 2313 28-29 -0.32864852280727064 2313 30-31 0.04889896372140612 2313 32-33 -2.1644306125564867 2313 34-35 -2.5555878881705283 2313 36-37 -1.1068680837189646 2313 38-39 -0.6438982910207187 2313 40-41 -0.5631287224537189 2313 42-43 -0.49333735070381834 2313 44-45 -0.10653489689571671 2313 46-47 -0.08010932116152247 2313 48-49 -0.19641042142404785 2313 50-51 -0.11280197021535798 2313 52-53 0.11088062816964595 2313 54-55 0.3445084764750561 2313 56-57 0.3617762645065419 2313 58-59 -0.09162323604864753 2313 60-61 0.47625682182337314 2313 62-63 0.33592557678607093 2313 64-65 0.09458271888681935 2313 66-67 1.4504400202653365 2313 68-69 4.594942956068589 2313 70-71 4.520592773318889 2313 72-73 2.5826318200472613 2313 74-75 1.7224969858329118 2313 76-77 0.9020527175630164 2313 78-79 0.6374486265059147 2313 80-81 0.7906195652049206 2313 82-83 0.4707243748263572 2313 84-85 0.6275332580169533 2313 86-87 0.924256068525608 2313 88-89 0.6689049668165836 2313 90-91 0.7912148368804388 2313 92-93 0.9552962493365591 2313 94-95 1.5758030782973584 2313 96-97 1.70073149391715 2313 98-99 1.4494952588492147 2313 100-101 1.2921501915936595 2314 1 -0.18193932674037328 2314 2 -0.1800308456043922 2314 3 -0.10437353575307284 2314 4 -0.09748125028001198 2314 5 -1.3984619794482214 2314 6 -2.4889745048371523 2314 7 -0.5773303419983478 2314 8 -0.2757976624321117 2314 9 0.10134290136215185 2314 10-11 0.3917251251926004 2314 12-13 0.07254328454811088 2314 14-15 -2.5757547531400107 2314 16-17 -2.666860530559248 2314 18-19 -0.2580429556945347 2314 20-21 0.23081072453954476 2314 22-23 0.14719702710660698 2314 24-25 -2.0007365549917466 2314 26-27 -1.0546291551735365 2314 28-29 -0.09265249801391917 2314 30-31 0.06541659252695808 2314 32-33 0.11985360150458035 2314 34-35 0.19581303404010697 2314 36-37 0.6013891371809592 2314 38-39 -1.1084982362974998 2314 40-41 -4.024806921935401 2314 42-43 -5.144640058725905 2314 44-45 -1.889836386082436 2314 46-47 -2.5801545027975337 2314 48-49 -0.5447839602596503 2314 50-51 -0.5107531724938355 2314 52-53 -1.9768980599344346 2314 54-55 -0.8764812209459549 2314 56-57 -3.2122782830396943 2314 58-59 -4.849396712232384 2314 60-61 -2.1515265612842533 2314 62-63 -1.0936031194798481 2314 64-65 -0.23080570715210413 2314 66-67 -0.23704841712244118 2314 68-69 2.1765732494130354 2314 70-71 2.175908485101637 2314 72-73 -0.19800599307605182 2314 74-75 0.14467856509529753 2314 76-77 0.09716732928466953 2314 78-79 0.4588958535777401 2314 80-81 0.7585157294492042 2314 82-83 0.5323590250408223 2314 84-85 0.5665545674254631 2314 86-87 0.45407964055271677 2314 88-89 0.5995530016777622 2314 90-91 0.9993481726493663 2314 92-93 1.2213873246322855 2314 94-95 1.0092168122709921 2314 96-97 0.9394095589227369 2314 98-99 0.5750024591501912 2314 100-101 0.5299419192150481 2315 1 -0.46441179852285686 2315 2 -0.43111381956447303 2315 3 -0.41591155294473836 2315 4 -0.23658806617152095 2315 5 -1.3941245012678891 2315 6 -0.5102005032022561 2315 7 -0.3696357416259346 2315 8 -0.227457995974504 2315 9 0.11610163748154889 2315 10-11 -2.0359839358675202 2315 12-13 -1.3225837209682112 2315 14-15 -0.27869319983206253 2315 16-17 -1.9148056226630032 2315 18-19 -3.1451134310380695 2315 20-21 -2.1901397003978786 2315 22-23 -1.540200306379468 2315 24-25 -1.2569744558765734 2315 26-27 -0.5484404031517656 2315 28-29 -0.41924814922543874 2315 30-31 -3.143409523892501 2315 32-33 -2.841684682668564 2315 34-35 -1.8286171061300251 2315 36-37 -4.889404856027035 2315 38-39 -2.5216984316308526 2315 40-41 -2.0446116079943053 2315 42-43 -1.992381856569473 2315 44-45 -2.034364503600891 2315 46-47 -1.6252105397827208 2315 48-49 -5.074736126887164 2315 50-51 -4.253752432712428 2315 52-53 -2.8762413097689006 2315 54-55 -4.526041453909137 2315 56-57 -6.770706572785468 2315 58-59 -7.178868724806726 2315 60-61 -5.909028740823956 2315 62-63 -7.124424432174379 2315 64-65 -6.573373161236145 2315 66-67 -4.560916488467658 2315 68-69 -2.33016232921835 2315 70-71 -0.012371343525376943 2315 72-73 -1.602239494106115 2315 74-75 -3.35775849038054 2315 76-77 -5.522925915210173 2315 78-79 -4.655302197166215 2315 80-81 -4.32831489944266 2315 82-83 -7.448057762172031 2315 84-85 -5.776521000953835 2315 86-87 -6.240021719208226 2315 88-89 -4.782971385866723 2315 90-91 -4.372164321112972 2315 92-93 -5.255899832879017 2315 94-95 -2.969727208023482 2315 96-97 -1.888158754356244 2315 98-99 -3.5063357539573374 2315 100-101 -2.5629196999159696 2316 1 -0.2704814158580362 2316 2 -0.3665264521140372 2316 3 -0.778396569081206 2316 4 -0.31626303159337965 2316 5 -0.3038893698709302 2316 6 -2.400511706521762 2316 7 -0.4687320993944937 2316 8 -0.3020177009076477 2316 9 -0.11843040493431545 2316 10-11 0.015297760766877389 2316 12-13 -0.5995028081051359 2316 14-15 -1.0260721763189196 2316 16-17 -1.466647485272503 2316 18-19 -0.2211254181288993 2316 20-21 -4.0702595713061385 2316 22-23 -4.236521191580017 2316 24-25 -2.621518024341306 2316 26-27 -2.2082467655307383 2316 28-29 -1.4050872455832035 2316 30-31 -0.42782861102943315 2316 32-33 -2.0828073198285466 2316 34-35 -2.6138510849220964 2316 36-37 -4.901178714948195 2316 38-39 -2.1964010595700003 2316 40-41 -1.65167246553235 2316 42-43 -3.0808558076990273 2316 44-45 -0.8206554210743775 2316 46-47 -0.8529110930330646 2316 48-49 -0.65332994892497 2316 50-51 -0.3486648347410082 2316 52-53 -2.6296656988883136 2316 54-55 -1.199824303148418 2316 56-57 -0.18087600570386186 2316 58-59 -0.25715941175923973 2316 60-61 -1.7028461682098488 2316 62-63 -1.1894463316672308 2316 64-65 -0.025863940397048424 2316 66-67 1.131124774787299 2316 68-69 3.5652964075266915 2316 70-71 3.985498642643364 2316 72-73 1.8913093763410949 2316 74-75 0.8999259264013872 2316 76-77 -0.22581088524244564 2316 78-79 0.15659499047785985 2316 80-81 -0.1987436684606365 2316 82-83 -0.28702848600789466 2316 84-85 0.06992897599406689 2316 86-87 -0.0599225951879383 2316 88-89 0.5645666500945481 2316 90-91 0.4418294548206383 2316 92-93 0.43846162401355926 2316 94-95 1.255419172797172 2316 96-97 1.162704546704152 2316 98-99 0.3578470491777459 2316 100-101 0.17904203360178172 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 33.0 4 106.0 5 240.0 6 587.0 7 1429.0 8 2968.0 9 4913.0 10 7964.0 11 11961.0 12 15783.0 13 19390.0 14 23876.0 15 30147.0 16 36340.0 17 41001.0 18 36678.0 19 30393.0 20 29080.0 21 29514.0 22 30783.0 23 35289.0 24 42588.0 25 52856.0 26 66578.0 27 82684.0 28 100298.0 29 119939.0 30 140800.0 31 164063.0 32 191414.0 33 220286.0 34 257374.0 35 294359.0 36 310184.0 37 264197.0 38 110766.0 39 6374.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.18802158293845 21.22007031236667 16.020489285328267 21.57141881936662 2 15.862059893362213 22.117972808323962 39.085127843398816 22.934839454915014 3 18.91828677315594 26.435950563780352 30.834649431029902 23.8111132320338 4 12.981859712694362 18.67365789137866 37.31155631529613 31.032926080630848 5 12.865229127758301 38.708582364638 34.05969784241197 14.366490665191733 6 29.92413376600156 37.92504801401657 17.54161836828057 14.609199851701293 7 26.83964864723641 34.47187780925985 20.900448983929923 17.788024559573813 8 26.08233706248416 35.21205613027821 19.727602434345297 18.978004372892332 9 26.17664110540121 17.046246659991095 20.809628467664165 35.96748376694354 10-11 24.298291543454685 27.94022979190194 26.956311243864285 20.805167420779096 12-13 25.690618045165703 25.500285969101117 27.806469477730488 21.00262650800269 14-15 22.66465972876124 26.606803327549965 27.042955082767527 23.685581860921264 16-17 20.80705352044187 29.864193640520774 27.704987872496183 21.62376496654117 18-19 20.599809363714517 27.59023898140576 31.321243113517454 20.488708541362264 20-21 21.81469699894179 26.578579652436463 31.464901158485887 20.141822190135862 22-23 21.289573129355073 26.69003473218256 31.347403226385513 20.672988912076853 24-25 20.904287972484408 26.944563227963762 31.57840014417535 20.572748655376486 26-27 20.867963622578117 27.125560543695613 31.409126560462475 20.597349273263795 28-29 20.598480104139764 27.183812616689274 31.662956489154688 20.55475079001628 30-31 20.880371304555084 27.024784148922805 31.520682547438184 20.57416199908393 32-33 20.68685902027708 27.176829261789727 31.205192075731812 20.93111964220138 34-35 21.098300932045102 27.18844673606465 31.198786044848227 20.514466287042023 36-37 21.159530545733645 26.896443011791867 31.211794926355545 20.732231516118944 38-39 20.946168345217757 27.20227765321442 31.218986421545896 20.632567580021927 40-41 21.102465246006773 27.087223705709366 31.262293932602823 20.548017115681034 42-43 20.930954161830254 27.212361197006135 30.8605234072928 20.996161233870815 44-45 20.972688394640368 27.445286589143176 30.68512608627118 20.89689892994527 46-47 21.199710501576657 27.584047412839123 30.05627712713379 21.159964958450427 48-49 21.172613574578197 27.762451286547545 29.860599107857063 21.204336031017196 50-51 21.353603830403042 27.953832911619415 29.53213773196786 21.160425526009686 52-53 21.554962017264945 27.989899730132972 29.357182141390968 21.097956111211115 54-55 21.220215572781377 27.96104003507215 29.47031628071592 21.34842811143055 56-57 21.299134157109112 28.254467274950724 29.078563089824133 21.367835478116035 58-59 21.2826951326861 27.94218864876472 29.330020861745076 21.4450953568041 60-61 21.32513632791719 28.018681977128512 29.449585199460287 21.20659649549401 62-63 21.357730156312403 27.94118614145093 29.564846621110874 21.136237081125795 64-65 21.215258584375626 27.861188970905815 29.425883498838473 21.497668945880086 66-67 20.890001781310094 27.95744775031958 29.73425994776014 21.418290520610185 68-69 21.251658181710013 27.540106808136166 30.062621614177964 21.145613395975854 70-71 20.836160380801726 27.51439529880614 30.356956778681415 21.29248754171072 72-73 21.253128490086684 27.91723568558715 29.248570635165922 21.58106518916025 74-75 20.946275726764522 28.24302391855296 29.18523056855647 21.62546978612605 76-77 21.054621535048582 28.262982569032392 29.03864818455566 21.64374771136337 78-79 21.066835407488643 28.245311709860584 29.02755695224925 21.66029593040152 80-81 20.9226214332674 28.492022345359903 28.86416063691136 21.721195584461334 82-83 21.176145236046253 28.448842456085348 28.638943481912854 21.73606882595554 84-85 21.062983939164827 28.35985256862103 28.61749135605517 21.95967213615897 86-87 20.998707893226126 28.347596272618535 28.70392981745215 21.94976601670319 88-89 21.095426796539474 28.240741728144393 28.585631949791235 22.078199525524898 90-91 20.88543913372446 28.38407100376749 28.73949152665885 21.9909983358492 92-93 20.99699326938099 28.276971130800515 28.72196441866587 22.004071181152632 94-95 20.562222669533416 28.08646208711049 29.02139196514298 22.329923278213116 96-97 20.54015517089035 28.029915431294693 29.02057638974002 22.409353008074927 98-99 20.737581290100675 28.02485386224078 28.591700388486025 22.645864459172525 100-101 20.628612980039883 27.915299296898066 28.84557583634328 22.610511886718772 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 6875.0 1 4438.0 2 2068.0 3 2273.5 4 2162.5 5 2064.0 6 3047.0 7 5479.5 8 8080.0 9 9364.5 10 8516.5 11 6868.0 12 6102.0 13 5803.5 14 5767.0 15 5737.0 16 5851.0 17 6061.0 18 6287.5 19 6756.0 20 7506.5 21 8704.0 22 10165.5 23 11823.5 24 13821.5 25 16730.0 26 20881.5 27 25614.5 28 30017.0 29 34496.0 30 39987.0 31 46490.5 32 53803.0 33 60864.0 34 67045.5 35 72711.5 36 80174.5 37 86484.0 38 88326.5 39 88622.0 40 88833.5 41 89698.5 42 90835.5 43 92666.0 44 94888.0 45 96616.0 46 97210.5 47 97360.5 48 100279.0 49 99576.0 50 93763.0 51 88980.0 52 85508.5 53 82449.5 54 78116.0 55 72508.0 56 66335.5 57 59976.0 58 54039.0 59 47192.0 60 39549.5 61 32268.5 62 25362.5 63 18898.0 64 13431.0 65 9649.5 66 6971.0 67 5153.5 68 3688.0 69 2611.5 70 2016.0 71 1555.0 72 1191.5 73 1012.0 74 825.5 75 624.0 76 453.0 77 260.5 78 162.0 79 109.0 80 69.0 81 42.0 82 29.5 83 22.0 84 11.5 85 6.0 86 3.0 87 0.5 88 1.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023069477517212354 2 0.019266035153049452 3 0.0 4 0.0 5 2.4882333223495603E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.6659642739459575E-4 18-19 0.0010841588047380227 20-21 0.0 22-23 0.0 24-25 0.0 26-27 4.4432737899099294E-4 28-29 0.017151036829052328 30-31 0.004709870217304526 32-33 0.009313101863651212 34-35 0.010201756621633197 36-37 0.10358159859038028 38-39 0.10592764715145271 40-41 0.12156797089193568 42-43 0.10532336191602498 44-45 0.10038244146164514 46-47 0.10176874288409703 48-49 0.10411479144516948 50-51 0.1025152128808019 52-53 0.07916136584103531 54-55 0.06730671136955561 56-57 0.037963331260990434 58-59 0.015533685169525115 60-61 0.009330874958810853 62-63 0.0021505445143164058 64-65 0.004834281883422003 66-67 0.42413714288964227 68-69 0.8143809933098515 70-71 0.9042417624369901 72-73 0.2834453216059342 74-75 0.15107130885693762 76-77 0.015320408027609436 78-79 0.00883322829434094 80-81 0.0014040745176115378 82-83 0.0 84-85 0.0 86-87 2.1327714191567664E-4 88-89 9.597471386205448E-4 90-91 0.0033591149851719066 92-93 0.0018661749917621704 94-95 0.22557612376614733 96-97 0.23208107659457544 98-99 0.014165156842232856 100-101 0.007340288300931203 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2813241.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.13294729465715 #Duplication Level Percentage of deduplicated Percentage of total 1 72.15051643522303 36.171180377293304 2 14.845747447965612 14.88522148717302 3 5.474400278495542 8.233434618950204 4 2.586389755725796 5.186533652289701 5 1.401278953378907 3.5125121957428536 6 0.8545575534047846 2.570489327105594 7 0.5620492346926782 1.9724029261895297 8 0.3844226784712054 1.5417793502934298 9 0.2657284661095501 1.198957606754415 >10 1.3060763319224917 11.82113436232025 >50 0.10615566751154829 3.6846914208870674 >100 0.05889109857447664 5.375896276971374 >500 0.0027859970275140923 0.9620772781851114 >1k 7.14358212167898E-4 0.7626353349695285 >5k 7.14358212167898E-5 0.2849272939665447 >10k+ 2.1430746365036938E-4 1.8361264909080879 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20457 0.7271684153614995 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18262 0.6491445276106811 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12551 0.4461402346972762 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7956 0.2828054901801872 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4956 0.1761669192223489 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3757 0.13354703702953283 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 3.5546190319279435E-5 0.0 0.0 0.0 0.0 8 3.5546190319279435E-5 0.0 0.0 0.0 0.0 9 3.5546190319279435E-5 0.0 0.0 0.0 0.0 10-11 3.5546190319279435E-5 0.0 0.0 7.109238063855887E-5 0.0 12-13 7.109238063855887E-5 0.0 0.0 1.066385709578383E-4 0.0 14-15 7.109238063855887E-5 0.0 0.0 1.4218476127711774E-4 0.0 16-17 7.109238063855887E-5 0.0 0.0 4.265542838313532E-4 0.0 18-19 7.109238063855887E-5 0.0 0.0 5.331928547891915E-4 0.0 20-21 7.109238063855887E-5 0.0 0.0 7.286969015452285E-4 0.0 22-23 7.109238063855887E-5 0.0 0.0 0.0011552511853765816 0.0 24-25 7.109238063855887E-5 0.0 0.0 0.0017239902304850526 0.0 26-27 7.109238063855887E-5 0.0 0.0 0.002186090704635685 0.0 28-29 7.109238063855887E-5 0.0 0.0 0.003998946410918937 0.0 30-31 1.4218476127711774E-4 0.0 0.0 0.008957639960458418 0.0 32-33 1.4218476127711774E-4 0.0 0.0 0.01644011302266674 0.0 34-35 1.777309515963972E-4 0.0 0.0 0.02884573344409526 0.0 36-37 2.4882333223495603E-4 0.0 0.0 0.05017344763566292 0.0 38-39 2.4882333223495603E-4 0.0 0.0 0.08093867535699928 0.0 40-41 2.4882333223495603E-4 0.0 0.0 0.11259255783631761 0.0 42-43 2.4882333223495603E-4 0.0 0.0 0.14561496864292822 0.0 44-45 2.843695225542355E-4 0.0 0.0 0.18841258178734066 0.0 46-47 3.9100809351207377E-4 0.0 0.0 0.23991901155997655 0.0 48-49 3.9100809351207377E-4 0.0 0.0 0.29601089988379947 0.0 50-51 3.9100809351207377E-4 0.0 0.0 0.3586610603215295 0.0 52-53 3.9100809351207377E-4 0.0 0.0 0.4197471883852112 0.0 54-55 4.265542838313532E-4 0.0 0.0 0.48964877164807424 0.0 56-57 4.265542838313532E-4 0.0 0.0 0.5680281213020855 0.0 58-59 4.265542838313532E-4 0.0 0.0 0.645394404531997 0.0 60-61 4.265542838313532E-4 0.0 0.0 0.7255688367971318 0.0 62-63 4.4432737899099294E-4 0.0 0.0 0.8055477650155105 0.0 64-65 4.6210047415063267E-4 0.0 0.0 0.89338240129445 0.0 66-67 4.6210047415063267E-4 0.0 0.0 0.987810855877616 0.0 68-69 4.6210047415063267E-4 0.0 0.0 1.1037803017942651 0.0 70-71 4.6210047415063267E-4 0.0 0.0 1.253394216848112 0.0 72-73 4.6210047415063267E-4 0.0 0.0 1.416195768510412 0.0 74-75 4.6210047415063267E-4 0.0 0.0 1.5935890312987762 0.0 76-77 4.6210047415063267E-4 0.0 0.0 1.7925588316109426 0.0 78-79 4.7987356931027234E-4 0.0 0.0 2.0229870103556715 0.0 80-81 5.68739045108471E-4 0.0 0.0 2.269784209742429 0.0 82-83 5.68739045108471E-4 0.0 0.0 2.527120854558852 0.0 84-85 6.042852354277504E-4 0.0 0.0 2.7880121184072038 0.0 86-87 6.398314257470299E-4 0.0 0.0 3.0646325714718365 0.0 88-89 6.398314257470299E-4 0.0 0.0 3.3615321261136177 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11140 0.0 45.685192 1 GTATCAA 18240 0.0 35.98167 1 ATCAACG 23610 0.0 27.665554 3 TATCAAC 23655 0.0 27.618324 2 TCAACGC 24045 0.0 27.125656 4 CAACGCA 24390 0.0 26.683699 5 AACGCAG 24960 0.0 26.074335 6 ACGCAGA 28280 0.0 22.929527 7 GTACATG 16785 0.0 22.867573 1 CGCAGAG 28440 0.0 22.750565 8 TACATGG 16630 0.0 22.36317 2 GAGTACT 16495 0.0 21.853003 12-13 GCAGAGT 29350 0.0 21.512611 9 ACATGGG 16975 0.0 21.37421 3 AGTACTT 17120 0.0 20.737036 12-13 GTACTTT 17550 0.0 20.336908 14-15 CATGGGA 11550 0.0 19.97184 4 AGAGTAC 27335 0.0 19.442175 10-11 CAGAGTA 28990 0.0 19.05933 10-11 TGGTATC 6100 0.0 18.794966 2 >>END_MODULE