FastQCFastQC Report
Fri 27 May 2016
SRR939428_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939428_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2793645
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144850.5184982343855429No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105580.3779291928645193No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75810.2713659036849707No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA49030.17550547761079166No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA90600.044.4641951
GTATCAA155150.040.584361
TATCAAC198450.031.4796852
ATCAACG202950.031.0781613
TCAACGC208550.030.5400894
CAACGCA210050.029.7829845
AACGCAG214950.028.8779856
GTACATG141600.026.105391
ACGCAGA237950.025.5567867
CGCAGAG238900.025.3112038
TACATGG144100.025.2382722
GCAGAGT232300.024.2564549
ACATGGG151550.023.0156763
GAGTACT131900.022.7608612-13
AGTACTT138050.021.46830412-13
GTACTTT143650.021.3709714-15
CATGGGA101900.020.7254314
AGAGTAC215200.020.34697710-11
CAGAGTA230700.020.17810-11
TGGTATC52450.019.6647132
Produced by FastQC (version 0.11.5)