FastQCFastQC Report
Fri 27 May 2016
SRR939428_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939428_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2793645
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200770.718666831326099No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175740.6290706227885074No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121990.4366696555933198No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA76470.2737284085844837No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47810.17113842309957064No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36590.1309758398078496No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA104600.047.1829341
GTATCAA172950.036.7403531
ATCAACG226050.027.933953
TATCAAC227450.027.8906562
TCAACGC229150.027.5590134
CAACGCA233650.027.0881145
AACGCAG240150.026.3746786
ACGCAGA273600.023.1154867
CGCAGAG274750.022.9152018
GTACATG158700.022.7856451
TACATGG160300.022.169792
GAGTACT162350.022.10306412-13
GCAGAGT283550.021.501799
GTACTTT171700.020.91323714-15
ACATGGG162850.020.4506993
AGTACTT168750.020.3237412-13
TATGCGA1200.00481816319.7538645
AGAGTAC267750.019.16613210-11
CAGAGTA281600.019.05679510-11
TGGTATC56350.018.9453162
Produced by FastQC (version 0.11.5)