##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939428_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2793645 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.866257881727993 31.0 31.0 31.0 28.0 34.0 2 31.113557019592683 31.0 31.0 34.0 28.0 34.0 3 31.102956531699625 31.0 31.0 34.0 28.0 34.0 4 35.025435944796136 35.0 35.0 37.0 32.0 37.0 5 34.59867878703271 35.0 35.0 37.0 30.0 37.0 6 34.66973863894661 35.0 35.0 37.0 31.0 37.0 7 34.51471822654632 35.0 35.0 37.0 30.0 37.0 8 34.413899761780755 35.0 35.0 37.0 30.0 37.0 9 35.90262685487956 37.0 35.0 39.0 30.0 39.0 10-11 35.708831651838366 37.0 35.0 39.0 30.0 39.0 12-13 35.692665854108164 37.0 35.0 39.0 30.0 39.0 14-15 36.475058749411616 38.0 35.0 40.0 30.0 41.0 16-17 36.398059524384806 38.0 35.0 40.0 30.0 41.0 18-19 36.44156236744468 38.0 35.0 40.0 30.0 41.0 20-21 36.342121851559526 38.0 34.5 40.0 29.5 41.0 22-23 36.17786243420334 38.0 34.0 40.0 29.0 41.0 24-25 36.0072088615411 38.0 34.0 40.0 29.0 41.0 26-27 35.53667627776615 38.0 34.0 40.0 27.0 41.0 28-29 35.32007216378602 38.0 34.0 40.0 27.0 41.0 30-31 34.99402483135832 37.0 33.0 40.0 27.0 41.0 32-33 34.70447354620934 37.0 33.0 40.0 25.5 41.0 34-35 34.90345641625905 37.0 33.0 40.0 26.0 41.0 36-37 34.71596104730558 37.0 33.0 40.0 25.0 41.0 38-39 34.50778821217442 37.0 33.0 40.0 25.0 41.0 40-41 34.32494357729776 37.0 33.0 40.0 24.5 41.0 42-43 34.1305561372329 37.0 33.0 40.0 24.0 41.0 44-45 33.70579959157301 37.0 32.5 40.0 23.5 41.0 46-47 33.24584709223971 37.0 31.0 40.0 21.0 41.0 48-49 32.8118452774064 36.5 31.0 39.5 18.5 41.0 50-51 32.54804798032678 36.0 31.0 39.0 18.0 40.0 52-53 32.43196308049161 36.0 31.0 39.0 17.5 40.0 54-55 32.098134337039966 36.0 30.5 39.0 15.0 40.0 56-57 31.604062613538943 35.5 29.5 39.0 12.0 40.0 58-59 31.156998473320698 35.0 29.0 38.5 9.0 40.0 60-61 30.798420164337273 35.0 28.0 38.0 8.0 40.0 62-63 30.637010071072023 34.5 28.0 38.0 8.0 40.0 64-65 30.19028312473489 34.0 28.0 38.0 7.0 40.0 66-67 29.329141139980205 33.5 27.0 37.5 2.0 39.5 68-69 25.92080776190246 31.0 16.5 36.0 2.0 39.0 70-71 24.61856051860562 29.5 12.0 35.0 2.0 38.0 72-73 25.622216494937618 30.5 18.5 35.0 2.0 38.0 74-75 26.360670915595932 31.0 21.5 35.0 2.0 37.5 76-77 26.04083750798688 30.5 22.0 34.0 2.0 36.0 78-79 27.141291932224746 32.0 25.5 35.0 2.0 37.0 80-81 27.147431044388245 32.0 25.5 35.0 2.0 36.5 82-83 26.82575398806935 32.0 25.0 35.0 2.0 36.0 84-85 26.447421916528405 32.0 24.5 34.5 2.0 36.0 86-87 25.989097576821678 32.0 23.5 34.0 2.0 35.0 88-89 25.493897578253502 31.0 21.0 34.0 2.0 35.0 90-91 24.881765399683925 31.0 19.5 34.0 2.0 35.0 92-93 24.312672512076517 31.0 17.5 34.0 2.0 35.0 94-95 23.46411856195043 30.0 7.0 34.0 2.0 35.0 96-97 22.781156875694656 30.0 2.0 34.0 2.0 35.0 98-99 21.919509995006525 29.5 2.0 34.0 2.0 35.0 100-101 20.5160471355523 27.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.298749130400747 1101 2 0.2762146404201431 1101 3 0.3645341138427902 1101 4 0.4031315222355616 1101 5 0.5153789979455112 1101 6 0.35725758537137153 1101 7 0.5310750303598653 1101 8 0.6709675657982999 1101 9 0.6057555617493691 1101 10-11 0.7454284878621564 1101 12-13 0.6516310489905166 1101 14-15 0.7095294646775656 1101 16-17 0.6785585519627304 1101 18-19 0.5503044609023746 1101 20-21 0.62425566273388 1101 22-23 0.7382513493138703 1101 24-25 0.7288151336008255 1101 26-27 0.7764380066658418 1101 28-29 0.5860021964345279 1101 30-31 0.4640587473805695 1101 32-33 0.9162354788061577 1101 34-35 0.8298070828510831 1101 36-37 0.9794408922434243 1101 38-39 1.043632409817583 1101 40-41 0.9232182884064812 1101 42-43 0.7372600919214349 1101 44-45 0.9218371245130541 1101 46-47 1.017005363636116 1101 48-49 0.9454771133530002 1101 50-51 0.7066543364646236 1101 52-53 0.5770343314329907 1101 54-55 0.4977856038515469 1101 56-57 0.6896900555999217 1101 58-59 0.7722855447910355 1101 60-61 0.535876686631557 1101 62-63 0.1749220248887582 1101 64-65 0.21293008915004563 1101 66-67 -0.07431135109903408 1101 68-69 -7.189201782530265 1101 70-71 -7.856653304725111 1101 72-73 -4.885729158511634 1101 74-75 -2.673380704856392 1101 76-77 0.04213213978462349 1101 78-79 0.1746800166222542 1101 80-81 0.01716972771831493 1101 82-83 -0.24430429547376775 1101 84-85 -0.3035461753652626 1101 86-87 -0.4244410753547463 1101 88-89 -0.39885407975024734 1101 90-91 -0.4634743867715301 1101 92-93 -0.3757419165340288 1101 94-95 -0.3339578618061161 1101 96-97 -0.4204259030242241 1101 98-99 -0.4373449090172983 1101 100-101 -0.43475795381268156 1102 1 0.34275549736804223 1102 2 0.34378371937870256 1102 3 0.36056071289905844 1102 4 0.3601459878219586 1102 5 0.47526629599034464 1102 6 0.45934339229049215 1102 7 0.5379068049797695 1102 8 0.6507939498439725 1102 9 0.5850120996705073 1102 10-11 0.6170903165068751 1102 12-13 0.6378805344279712 1102 14-15 0.8174605532707702 1102 16-17 0.8470529079345468 1102 18-19 0.6081141807762194 1102 20-21 0.6161839412202355 1102 22-23 0.7165582854468795 1102 24-25 0.6571559202946702 1102 26-27 0.7177647998099985 1102 28-29 0.6572595406454056 1102 30-31 0.6815811329775769 1102 32-33 0.6236760532110495 1102 34-35 0.4107788150812439 1102 36-37 0.6258134176653627 1102 38-39 0.6881025422260052 1102 40-41 0.5984412938599988 1102 42-43 0.40080444125921844 1102 44-45 0.6355020983681712 1102 46-47 0.7216170135022395 1102 48-49 0.8458708812776408 1102 50-51 0.6669786937292272 1102 52-53 0.5090802873966496 1102 54-55 0.29177814992403484 1102 56-57 0.3438171661568781 1102 58-59 0.4641287078282659 1102 60-61 0.3988154043587038 1102 62-63 0.16504401873782015 1102 64-65 0.1648088165390611 1102 66-67 -0.019404657023052607 1102 68-69 -7.013694066643634 1102 70-71 -7.4519710194550335 1102 72-73 -1.56723078384886 1102 74-75 0.05172435520709229 1102 76-77 0.33228268972866104 1102 78-79 0.023752336899587334 1102 80-81 -0.044735548546487536 1102 82-83 0.08648372516997327 1102 84-85 -0.33671292084646964 1102 86-87 -0.4312089549147906 1102 88-89 -0.28215724809469833 1102 90-91 -0.22600940679269144 1102 92-93 -0.2104873909351781 1102 94-95 -0.05328674466956684 1102 96-97 -0.314407732587501 1102 98-99 -0.30022355900930364 1102 100-101 -0.3609558639232837 1103 1 0.38249924885657904 1103 2 0.23965057074848772 1103 3 0.34965954405642563 1103 4 0.25851368026545174 1103 5 0.43552087900931724 1103 6 0.425055898307086 1103 7 0.33954477521076143 1103 8 0.4790659555799266 1103 9 0.4310557430685762 1103 10-11 0.5753825850408916 1103 12-13 0.4499235649248092 1103 14-15 0.6236125806747808 1103 16-17 0.6463930189895848 1103 18-19 0.6087726228245813 1103 20-21 0.546244030107573 1103 22-23 0.6046300073829158 1103 24-25 0.6011886064176153 1103 26-27 0.7133524417724146 1103 28-29 0.7520663476797793 1103 30-31 0.6046052967754392 1103 32-33 0.8214181033990045 1103 34-35 0.7847759804130163 1103 36-37 0.8772183126310793 1103 38-39 0.9009899045522332 1103 40-41 0.8230993682508867 1103 42-43 0.6854823891392954 1103 44-45 0.8370789970712522 1103 46-47 0.8457095803259662 1103 48-49 1.0019896196504234 1103 50-51 0.6322853920902176 1103 52-53 0.4188342754184262 1103 54-55 0.6082552419075142 1103 56-57 0.8128696612311863 1103 58-59 0.8492456378925688 1103 60-61 0.9894902806542483 1103 62-63 0.6016244066476766 1103 64-65 0.45855313304592116 1103 66-67 0.4711905670939487 1103 68-69 -6.596348399505633 1103 70-71 -7.44418197947822 1103 72-73 -0.7422215299561934 1103 74-75 0.7446183128835955 1103 76-77 1.0478914147441891 1103 78-79 0.8227934400052348 1103 80-81 0.6005709686921925 1103 82-83 0.7278319913090101 1103 84-85 0.4224626936786251 1103 86-87 0.4891837827884622 1103 88-89 0.5761913661322744 1103 90-91 0.3455479057186537 1103 92-93 0.4795594593686907 1103 94-95 0.5550711338593999 1103 96-97 0.5710174176815599 1103 98-99 0.8710576668931012 1103 100-101 0.8530843835355917 1104 1 0.2579190180984057 1104 2 0.2726216207839336 1104 3 0.20113048704801528 1104 4 0.21730796541918096 1104 5 0.4048662333368185 1104 6 0.2658832270045224 1104 7 0.4857083789424266 1104 8 0.5777273829588481 1104 9 0.618456526987444 1104 10-11 0.5106185843523576 1104 12-13 0.47671614848224664 1104 14-15 0.5930965984409795 1104 16-17 0.6523867037105688 1104 18-19 0.5811084184750825 1104 20-21 0.4843839410544888 1104 22-23 0.5115882281968069 1104 24-25 0.424968044249205 1104 26-27 0.533828944257877 1104 28-29 0.4359581291178145 1104 30-31 0.5517314931524808 1104 32-33 0.8106168539164855 1104 34-35 0.4251004497747246 1104 36-37 0.6993771311630539 1104 38-39 0.6740356446694946 1104 40-41 0.7641917770214448 1104 42-43 0.6463405847192192 1104 44-45 0.7725576188399614 1104 46-47 0.7774040321226039 1104 48-49 0.9133142734854047 1104 50-51 0.7900297837016836 1104 52-53 0.5668328946452306 1104 54-55 0.6749778058268916 1104 56-57 0.7035413290361525 1104 58-59 0.7224495684689209 1104 60-61 0.8218299878785054 1104 62-63 0.638626876368459 1104 64-65 0.5655343667464159 1104 66-67 0.5621718291749431 1104 68-69 -6.304491167011928 1104 70-71 -3.873800489983015 1104 72-73 0.6154578024356532 1104 74-75 1.2333154688486516 1104 76-77 1.4244386920358991 1104 78-79 0.8466617520007453 1104 80-81 0.6778415169479963 1104 82-83 0.5214458844292658 1104 84-85 0.6386093801622863 1104 86-87 0.40950968351469186 1104 88-89 0.47672695559224465 1104 90-91 0.523001168890449 1104 92-93 0.35885332159121575 1104 94-95 0.6580522589736191 1104 96-97 0.38944620513688477 1104 98-99 0.5646617502305986 1104 100-101 0.8099794885773868 1105 1 0.1542654782095667 1105 2 0.28057597500938414 1105 3 0.13220575770092324 1105 4 0.1659483395083683 1105 5 0.3721244870615692 1105 6 0.3337549769136743 1105 7 0.2716196319412134 1105 8 0.38438950520933446 1105 9 0.4465184594943281 1105 10-11 0.42305870008457447 1105 12-13 0.30790500921580843 1105 14-15 0.37298700809692775 1105 16-17 0.44359212026444084 1105 18-19 0.45948848043848045 1105 20-21 0.6070686463706352 1105 22-23 0.5130607938246001 1105 24-25 0.5212537766521947 1105 26-27 0.55654289561555 1105 28-29 0.47875612372142484 1105 30-31 0.4991629021409949 1105 32-33 0.5559523236944912 1105 34-35 0.5796342772218566 1105 36-37 0.5895548918145863 1105 38-39 0.4052045676916407 1105 40-41 0.5502834043207869 1105 42-43 0.4620025208031038 1105 44-45 0.5620647732468669 1105 46-47 0.6173771721028203 1105 48-49 0.6861093170638313 1105 50-51 0.5187430757531786 1105 52-53 0.2289532143640045 1105 54-55 0.3938390027724523 1105 56-57 0.5997262144217466 1105 58-59 0.7410184675901981 1105 60-61 0.7420543470873717 1105 62-63 0.419292336610507 1105 64-65 0.4126862006500325 1105 66-67 0.587985881257687 1105 68-69 -3.3769648496393323 1105 70-71 -3.300281855844286 1105 72-73 0.7554893328795806 1105 74-75 1.2733707578905928 1105 76-77 1.3512736931455827 1105 78-79 1.0098996810476955 1105 80-81 0.8235647457435 1105 82-83 0.8181638444660457 1105 84-85 0.9284550285382878 1105 86-87 0.7077613840124677 1105 88-89 0.7036426017976574 1105 90-91 0.5435805047228044 1105 92-93 0.4494850564119339 1105 94-95 0.5225320168107039 1105 96-97 0.6251599508170465 1105 98-99 0.5756031028732309 1105 100-101 0.6340755237165361 1106 1 0.08537331392723502 1106 2 0.17804483262240112 1106 3 0.17670659774273645 1106 4 0.19933501731255632 1106 5 0.2655980589390978 1106 6 0.2628964118374668 1106 7 0.24188169950055283 1106 8 0.2942646520162526 1106 9 0.21124991948165217 1106 10-11 0.33698175085664417 1106 12-13 0.17983681146828445 1106 14-15 0.3353641003395609 1106 16-17 0.361593597685669 1106 18-19 0.15502660871761265 1106 20-21 0.1868132691369908 1106 22-23 0.2822310433953277 1106 24-25 0.34824585966402566 1106 26-27 0.58138455790764 1106 28-29 0.48215554911391934 1106 30-31 0.3982140577483122 1106 32-33 0.5710200470557893 1106 34-35 0.516781031108728 1106 36-37 0.5967509485715681 1106 38-39 0.6585479834451249 1106 40-41 0.38732675105395487 1106 42-43 0.3665380477685076 1106 44-45 0.5148828699715082 1106 46-47 0.6356473108052967 1106 48-49 0.7416420005894295 1106 50-51 0.5806369641046984 1106 52-53 0.4380217298763185 1106 54-55 0.40063107073876125 1106 56-57 0.44972304910637106 1106 58-59 0.7961838512079247 1106 60-61 0.9034258248004683 1106 62-63 0.426526932109919 1106 64-65 0.4783209162670019 1106 66-67 0.5700658284914724 1106 68-69 -2.867391517709155 1106 70-71 -3.1291868478755056 1106 72-73 0.8956602373006213 1106 74-75 1.3427344308403413 1106 76-77 1.3302329271721476 1106 78-79 0.9865538871968766 1106 80-81 0.7447324120880161 1106 82-83 0.5736372194362929 1106 84-85 0.3882021342273454 1106 86-87 0.49077387632013014 1106 88-89 0.4264774644168057 1106 90-91 0.6898943305326242 1106 92-93 0.5111899507705608 1106 94-95 0.6713036380729065 1106 96-97 0.8152066166094833 1106 98-99 0.8098233890917328 1106 100-101 0.9197277143408726 1107 1 0.11167358479162814 1107 2 0.14033105569680515 1107 3 0.1906966240187593 1107 4 -0.013078857100076391 1107 5 0.29852509927519577 1107 6 0.18493910913556277 1107 7 0.13164094767566326 1107 8 0.083811618548431 1107 9 0.3882216611718192 1107 10-11 0.1390356387963365 1107 12-13 0.3238599591983302 1107 14-15 0.1603732444645516 1107 16-17 0.055048311689553486 1107 18-19 0.25212449945841087 1107 20-21 0.2575772653599273 1107 22-23 0.39921134402958813 1107 24-25 0.29782056355495 1107 26-27 0.5882860283174267 1107 28-29 0.4030061154677185 1107 30-31 0.2638969379849456 1107 32-33 0.31997135547931777 1107 34-35 0.3750642828005155 1107 36-37 0.32313297233185523 1107 38-39 0.43797708613973896 1107 40-41 0.31121438468372986 1107 42-43 0.1958186891009035 1107 44-45 0.27787684939770685 1107 46-47 0.33165278844330004 1107 48-49 0.22558956191542023 1107 50-51 0.33951662612663114 1107 52-53 0.019985474094291078 1107 54-55 0.14527176212729387 1107 56-57 0.4308661067595132 1107 58-59 0.5198814788651873 1107 60-61 0.5054587949480513 1107 62-63 0.11863529898967684 1107 64-65 -0.013812926996337183 1107 66-67 0.21448594009182642 1107 68-69 -2.8241561333832514 1107 70-71 -2.8317095486911548 1107 72-73 1.0783978428352476 1107 74-75 1.368563755019995 1107 76-77 0.8598621013816832 1107 78-79 0.6279401106808002 1107 80-81 0.47834793983604484 1107 82-83 0.47976989363106526 1107 84-85 0.44001710024482676 1107 86-87 0.41267200268515225 1107 88-89 0.5422896663975401 1107 90-91 0.5413649127455784 1107 92-93 0.5944647480533369 1107 94-95 0.7979831772822905 1107 96-97 0.5693515401598574 1107 98-99 0.6894122364707371 1107 100-101 0.8955961152408669 1108 1 -0.008218154613167883 1108 2 0.0032657557376190027 1108 3 -0.028810046441140713 1108 4 -0.016080919389288795 1108 5 -0.10488831537352894 1108 6 -0.1919289591778508 1108 7 -0.037571675673078175 1108 8 0.13316532855957064 1108 9 0.13545248347269734 1108 10-11 0.1447574140751655 1108 12-13 0.06578153174518064 1108 14-15 0.10418798993393352 1108 16-17 0.23224178672470686 1108 18-19 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1.0958093725507005 2312 90-91 1.372555879198238 2312 92-93 1.6049353080201918 2312 94-95 1.9051295832398836 2312 96-97 1.7775863177888418 2312 98-99 1.855421493854898 2312 100-101 2.0615782791885557 2313 1 -0.24309860079862133 2313 2 -0.1046801548827645 2313 3 -0.06883128179280718 2313 4 0.03801812772678659 2313 5 0.05802257404188538 2313 6 0.039333080310846924 2313 7 -2.696235356647829 2313 8 -2.955324878816569 2313 9 -0.8475463912304448 2313 10-11 0.003469681136785141 2313 12-13 0.03567307345712578 2313 14-15 -0.2708555828642645 2313 16-17 -0.020972242307614408 2313 18-19 0.2658063787493248 2313 20-21 -2.432687816211569 2313 22-23 -0.5541729142082659 2313 24-25 0.09141018243871457 2313 26-27 -1.383017632211235 2313 28-29 -1.5630729150426603 2313 30-31 -0.7251572597059877 2313 32-33 -0.6752392871265371 2313 34-35 -1.2543533811168501 2313 36-37 -1.9298947807810833 2313 38-39 -1.7371152666473648 2313 40-41 -0.75895226875992 2313 42-43 0.028860527440322414 2313 44-45 -0.05523386635133676 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68-69 -1.19965773696536 2314 70-71 1.6679293108147668 2314 72-73 1.1856777675647798 2314 74-75 0.7982669972559648 2314 76-77 0.7335222969102482 2314 78-79 -1.482049635408135 2314 80-81 -1.0001932159543863 2314 82-83 0.16439508360307897 2314 84-85 0.25791841480185695 2314 86-87 -0.01370047908528349 2314 88-89 0.042381853001817404 2314 90-91 0.4339961113967945 2314 92-93 -0.14550803964422343 2314 94-95 0.36179215311658197 2314 96-97 0.5476443766480017 2314 98-99 0.3188533560626112 2314 100-101 0.3587487280867059 2315 1 -0.41470870922672987 2315 2 -0.3595006230662321 2315 3 -0.40438486464721635 2315 4 -0.007791451530643201 2315 5 -0.22826063602965263 2315 6 -0.10171136887320387 2315 7 -0.9723689232466697 2315 8 -2.804238868406088 2315 9 -0.7331132321737286 2315 10-11 -1.2861730854228526 2315 12-13 -0.6598913032109479 2315 14-15 -0.3769664089584879 2315 16-17 -0.17597479304863128 2315 18-19 -0.07374732803938855 2315 20-21 -0.4772742647154331 2315 22-23 -0.24383095639204555 2315 24-25 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0.18982823314809139 2315 90-91 0.23303586591460856 2315 92-93 -0.923111432019784 2315 94-95 0.0737581023616869 2315 96-97 0.1701809636537348 2315 98-99 -2.0086034268208692 2315 100-101 -3.4679321450708684 2316 1 -0.5142865754123704 2316 2 -0.45981197249419026 2316 3 -1.0331665658670808 2316 4 -0.3683843333249399 2316 5 -0.3398536660855811 2316 6 -0.5096989643166339 2316 7 -0.7659275730774056 2316 8 -1.0470720658329213 2316 9 -5.150052123670921 2316 10-11 -1.5943955314103206 2316 12-13 -0.7751267303279974 2316 14-15 -0.4333540142860244 2316 16-17 -0.2903039709378774 2316 18-19 -0.5140233211864071 2316 20-21 -3.7414358245925357 2316 22-23 -1.2829457726711695 2316 24-25 -0.48308330938756683 2316 26-27 -0.5778992188138616 2316 28-29 -0.001110176856435885 2316 30-31 0.04397968002638919 2316 32-33 -1.2768816360772064 2316 34-35 -0.47649970950298126 2316 36-37 -0.40702842744979506 2316 38-39 -5.240566899299651 2316 40-41 -4.8056901964113905 2316 42-43 -3.648126528461905 2316 44-45 -2.3136051307584893 2316 46-47 -2.6427797188672884 2316 48-49 -2.993803336991135 2316 50-51 -1.4343428366402264 2316 52-53 -0.9443140837762201 2316 54-55 -0.22825427878715487 2316 56-57 -4.049476446534406 2316 58-59 -1.0372929922333327 2316 60-61 -0.5234089063047271 2316 62-63 -0.25349446997553926 2316 64-65 -0.001336318550375637 2316 66-67 -0.2958202427326775 2316 68-69 2.705719831202906 2316 70-71 3.839870547872284 2316 72-73 2.264584906937035 2316 74-75 0.9730216999176449 2316 76-77 -1.3367655547729562 2316 78-79 -0.0037496408290742522 2316 80-81 0.11051313196125889 2316 82-83 0.4461048467081916 2316 84-85 0.5352643247059099 2316 86-87 0.4370828461166454 2316 88-89 0.12076858255133871 2316 90-91 0.07552589943821175 2316 92-93 0.07708099922388456 2316 94-95 0.557363248687679 2316 96-97 0.7593156511099757 2316 98-99 0.8274726437516655 2316 100-101 0.9595347637564622 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 52.0 4 99.0 5 239.0 6 641.0 7 1569.0 8 3271.0 9 5450.0 10 8726.0 11 12753.0 12 16368.0 13 20830.0 14 25425.0 15 32353.0 16 38091.0 17 42596.0 18 38628.0 19 33088.0 20 32190.0 21 32589.0 22 34344.0 23 38484.0 24 45486.0 25 55879.0 26 69432.0 27 86127.0 28 105563.0 29 125169.0 30 145835.0 31 168816.0 32 194863.0 33 224724.0 34 257422.0 35 285693.0 36 284617.0 37 228888.0 38 92420.0 39 4920.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.926635811983346 21.321953833975453 16.158502420076953 21.59290793396425 2 15.841320429865767 22.283900870379007 39.00032615494114 22.874452544814083 3 18.852172356704997 26.527432994764865 30.96593136158217 23.654463286947962 4 12.91076582657621 18.749552152391434 37.41680782823288 30.922874192799473 5 12.79697313008632 38.74851672277616 34.13679977234044 14.317710374797086 6 29.91392965104729 38.033751604087136 17.52527611776013 14.52704262710545 7 26.734320216061814 34.4608566943903 20.9864889776618 17.818334111886085 8 26.050947776113286 35.22895715096227 19.775347261373582 18.94474781155086 9 26.121787127569895 17.038886472690695 20.862994403369076 35.976331996370334 10-11 24.243618103260943 28.022583073756746 26.96995092955328 20.763847893429027 12-13 25.639836128069245 25.561372328982383 27.838182732594873 20.9606088103535 14-15 22.57998063461893 26.625931354914457 27.088445382287297 23.705642628179312 16-17 20.860031725753185 29.890058792692447 27.691431523041977 21.558477958512395 18-19 20.540209189444596 27.655245486175744 31.321197434171903 20.483347890207757 20-21 21.795646905745002 26.61957764855592 31.48184540269075 20.102930043008328 22-23 21.27824653109322 26.711409972546562 31.365911743444347 20.644431752915864 24-25 20.832779224675058 26.978794358598122 31.607730088735543 20.58069632799128 26-27 20.873087935416816 27.186518775698758 31.36688891120224 20.573504377682188 28-29 20.658015854393447 27.206584454060433 31.599519689663254 20.535880001882866 30-31 20.873100731267904 27.069637795264317 31.482516901778002 20.574744571689777 32-33 20.695450379318512 27.203402313514484 31.170858974269482 20.930288332897522 34-35 21.027509050690128 27.227851515524126 31.201617714098244 20.543021719687506 36-37 21.117398254678754 26.933714622278178 31.194930270984088 20.753956852058984 38-39 20.941053298776236 27.20979140872607 31.256354273914866 20.59280101858283 40-41 21.04970063977434 27.125891068199966 31.2570908412214 20.567317450804296 42-43 20.833988632720068 27.22309495959833 30.91357392224895 21.02934248543265 44-45 20.992578419362566 27.438264102803387 30.667519528377746 20.9016379494563 46-47 21.169023827632 27.60810525670957 30.056390593614797 21.166480322043633 48-49 21.155444714085185 27.764492762059547 29.862717258665317 21.217345265189948 50-51 21.341581910683598 27.944981224874333 29.549567137753808 21.16386972668826 52-53 21.4577361703225 28.018395993439878 29.42746490803615 21.09640292820147 54-55 21.220456437850856 28.02426336947471 29.44521939995165 21.31006079272278 56-57 21.313932488037388 28.243296854702766 29.108697582556715 21.334073074703134 58-59 21.302466114599376 27.946625459136126 29.30463724352555 21.44627118273895 60-61 21.30551437032057 28.011371599891095 29.506194253375384 21.17691977641295 62-63 21.32464844027561 27.964679096208407 29.568728228032313 21.14194423548367 64-65 21.188241339741385 27.841950380969582 29.442561378531927 21.527246900757106 66-67 21.001642701343844 28.097972687132433 29.443413922019367 21.456970689504352 68-69 21.506194775778756 27.53978235175769 29.760577558910047 21.19344531355351 70-71 21.06400955301241 27.44047921076776 30.188035657308614 21.307475578911216 72-73 21.27693626113945 27.876624474180577 29.311422866137278 21.535016398542695 74-75 21.1377207550089 28.225052906708342 28.92760456976433 21.70962176851842 76-77 20.532510995646216 28.299079425095286 29.493043592720475 21.675365986538022 78-79 21.075893558772766 28.256709678828283 29.00102425820042 21.666372504198527 80-81 20.92543454973551 28.487078728608108 28.83486170386989 21.752625017786492 82-83 21.19104867309064 28.53015306073138 28.577242887932652 21.701555378245324 84-85 21.07184533440243 28.300210478085937 28.64139617130339 21.986548016208243 86-87 20.991474787335033 28.345401410941314 28.67102114980119 21.99210265192247 88-89 21.053913628659433 28.25447848412552 28.566831386347896 22.12477650086715 90-91 20.891086751972377 28.389507651901013 28.681039326169493 22.038366269957113 92-93 20.99509812607626 28.283331069167666 28.68096509658859 22.040605708167483 94-95 20.758303634901747 28.141342384033724 28.76750359614169 22.33285038492284 96-97 20.825087654999805 28.006307328464676 28.685363893355515 22.483241123180004 98-99 20.711708850903047 28.0156576734808 28.530163468316445 22.742470007299705 100-101 20.617158826149158 27.92073657092696 28.827774492100293 22.634330110823594 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 6575.0 1 4360.5 2 2167.5 3 2257.5 4 2092.0 5 1994.5 6 2999.5 7 5476.5 8 8101.5 9 9395.5 10 8477.5 11 6623.5 12 5749.5 13 5466.5 14 5444.5 15 5514.5 16 5674.0 17 6005.0 18 6374.0 19 6775.0 20 7514.0 21 8660.5 22 10096.0 23 11859.5 24 13885.0 25 16757.5 26 20963.5 27 25968.5 28 29998.5 29 34118.5 30 39996.5 31 46368.0 32 53537.0 33 60382.5 34 66550.0 35 72299.5 36 79493.0 37 86098.0 38 88329.5 39 88845.5 40 88933.5 41 89286.5 42 89856.5 43 91259.5 44 93743.5 45 95874.5 46 96123.5 47 96169.5 48 99374.0 49 98740.5 50 92970.5 51 88177.0 52 84250.5 53 81112.5 54 77431.5 55 71922.0 56 65554.0 57 59346.0 58 53701.5 59 47298.5 60 39614.0 61 32370.0 62 25665.5 63 18950.0 64 13460.5 65 9730.0 66 6981.0 67 5157.0 68 3701.0 69 2572.5 70 1969.5 71 1531.0 72 1214.0 73 1029.5 74 840.5 75 628.0 76 418.0 77 252.0 78 163.5 79 107.0 80 77.5 81 55.5 82 32.0 83 15.5 84 8.5 85 8.5 86 6.5 87 2.0 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.019759131886836017 2 0.01768299121756701 3 7.159105756100006E-4 4 0.09009734594051856 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.01123979603707701 12-13 0.0 14-15 0.0 16-17 5.548306960977505E-4 18-19 1.7897764390250014E-4 20-21 0.0 22-23 1.9687540829275017E-4 24-25 0.004277565689269754 26-27 0.0035974506424402528 28-29 0.001234945742927251 30-31 1.4318211512200012E-4 32-33 5.906262248782504E-4 34-35 0.0026309713653667522 36-37 0.08630301988978556 38-39 0.09695218970198433 40-41 0.07846379908685606 42-43 0.08232971619515007 44-45 0.08084420175075932 46-47 0.07953766495027106 48-49 0.07411464234002531 50-51 0.0734703228219763 52-53 0.05813193873953204 54-55 0.04903987442928504 56-57 0.02781312586244852 58-59 0.02247959207415402 60-61 0.01326224341317526 62-63 0.0020940384336592516 64-65 0.0064610929448802555 66-67 0.15524520832102862 68-69 0.72231797526171 70-71 0.8832725704232285 72-73 0.358438527443537 74-75 0.052476245192213035 76-77 0.19673222617762817 78-79 0.014461393627322012 80-81 0.0033110864121962524 82-83 0.0017181853814640011 84-85 1.7897764390250014E-4 86-87 0.0017539809102445011 88-89 3.579552878050003E-4 90-91 0.004420747804391753 92-93 0.004349156746830753 94-95 0.07558225902002581 96-97 0.03652933712050028 98-99 0.013673891994151012 100-101 0.013494914350248511 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2793645.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.06679275583335 #Duplication Level Percentage of deduplicated Percentage of total 1 72.9663798555953 37.261589982290985 2 14.470464309409934 14.779204039386402 3 5.24159213358847 8.03013897589706 4 2.538310194673838 5.184934426457115 5 1.3664887022255756 3.4891097679870566 6 0.8256340943373109 2.5297491112604167 7 0.538571756710897 1.925219259887033 8 0.3661262117495227 1.4957513102312983 9 0.26178143173946644 1.20315043097682 >10 1.2656887922837015 11.692782566772179 >50 0.09969362195596154 3.5258684560408633 >100 0.055592123170360434 5.147863518716236 >500 0.0027575794198604303 0.9477596661491903 >1k 6.363644814940633E-4 0.7107836417201331 >5k 7.070716461045148E-5 0.2761169676522153 >10k+ 2.1212149383135443E-4 1.7999778785749876 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20077 0.718666831326099 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17574 0.6290706227885074 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12199 0.4366696555933198 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7647 0.2737284085844837 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4781 0.17113842309957064 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3659 0.1309758398078496 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 3.579552878050003E-5 0.0 4 0.0 0.0 0.0 3.579552878050003E-5 0.0 5 0.0 0.0 0.0 3.579552878050003E-5 0.0 6 0.0 0.0 0.0 3.579552878050003E-5 0.0 7 0.0 0.0 0.0 7.159105756100006E-5 0.0 8 0.0 0.0 0.0 1.0738658634150008E-4 0.0 9 0.0 0.0 0.0 1.4318211512200012E-4 0.0 10-11 0.0 0.0 0.0 1.6107987951225015E-4 0.0 12-13 7.159105756100006E-5 0.0 0.0 2.1477317268300017E-4 0.0 14-15 7.159105756100006E-5 0.0 0.0 3.2215975902450024E-4 0.0 16-17 8.948882195125007E-5 0.0 0.0 5.369329317075004E-4 0.0 18-19 1.0738658634150008E-4 0.0 0.0 6.622172824392505E-4 0.0 20-21 1.0738658634150008E-4 0.0 0.0 7.696038687807507E-4 0.0 22-23 1.0738658634150008E-4 0.0 0.0 0.001342332329268751 0.0 24-25 1.0738658634150008E-4 0.0 0.0 0.0020761406692690015 0.0 26-27 1.0738658634150008E-4 0.0 0.0 0.0027383579517082524 0.0 28-29 2.1477317268300017E-4 0.0 0.0 0.004599725448294253 0.0 30-31 2.505687014635002E-4 0.0 0.0 0.009539508420003256 0.0 32-33 2.505687014635002E-4 0.0 0.0 0.018613674965860014 0.0 34-35 2.8636423024400024E-4 0.0 0.0 0.03216228260927927 0.0 36-37 2.8636423024400024E-4 0.0 0.0 0.05369329317075004 0.0 38-39 3.2215975902450024E-4 0.0 0.0 0.08354676417368706 0.0 40-41 3.2215975902450024E-4 0.0 0.0 0.11420563457418534 0.0 42-43 3.400575234147503E-4 0.0 0.0 0.14581308648736685 0.0 44-45 3.579552878050003E-4 0.0 0.0 0.18849925455811314 0.0 46-47 3.937508165855003E-4 0.0 0.0 0.23870248367276442 0.0 48-49 4.653418741465004E-4 0.0 0.0 0.29370231364400273 0.0 50-51 4.653418741465004E-4 0.0 0.0 0.35478738350792605 0.0 52-53 4.653418741465004E-4 0.0 0.0 0.4147448942152636 0.0 54-55 5.011374029270004E-4 0.0 0.0 0.48372287817528714 0.0 56-57 5.011374029270004E-4 0.0 0.0 0.5661420831923885 0.0 58-59 5.011374029270004E-4 0.0 0.0 0.6514070327475395 0.0 60-61 5.011374029270004E-4 0.0 0.0 0.7345600461046411 0.0 62-63 5.011374029270004E-4 0.0 0.0 0.8193059604924748 1.7897764390250015E-5 64-65 5.011374029270004E-4 0.0 0.0 0.9124817219081165 3.579552878050003E-5 66-67 5.011374029270004E-4 0.0 0.0 1.0142842057598584 3.579552878050003E-5 68-69 5.011374029270004E-4 0.0 0.0 1.130727060882825 3.579552878050003E-5 70-71 5.369329317075004E-4 0.0 3.579552878050003E-5 1.2727279235550686 3.579552878050003E-5 72-73 5.369329317075004E-4 0.0 3.579552878050003E-5 1.430872569707318 7.159105756100006E-5 74-75 5.369329317075004E-4 0.0 3.579552878050003E-5 1.5989146795673752 7.159105756100006E-5 76-77 5.369329317075004E-4 1.7897764390250015E-5 3.579552878050003E-5 1.7974545799484187 7.159105756100006E-5 78-79 5.369329317075004E-4 3.579552878050003E-5 3.579552878050003E-5 2.028693695870449 7.159105756100006E-5 80-81 5.727284604880005E-4 3.579552878050003E-5 3.579552878050003E-5 2.2791371129832174 7.159105756100006E-5 82-83 6.443195180490005E-4 3.579552878050003E-5 3.579552878050003E-5 2.5378313994798907 7.159105756100006E-5 84-85 6.443195180490005E-4 3.579552878050003E-5 3.579552878050003E-5 2.805868318988275 7.159105756100006E-5 86-87 6.443195180490005E-4 3.579552878050003E-5 3.579552878050003E-5 3.0897626577464212 1.252843507317501E-4 88-89 6.443195180490005E-4 3.579552878050003E-5 3.579552878050003E-5 3.400915291670917 1.4318211512200012E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10460 0.0 47.182934 1 GTATCAA 17295 0.0 36.740353 1 ATCAACG 22605 0.0 27.93395 3 TATCAAC 22745 0.0 27.890656 2 TCAACGC 22915 0.0 27.559013 4 CAACGCA 23365 0.0 27.088114 5 AACGCAG 24015 0.0 26.374678 6 ACGCAGA 27360 0.0 23.115486 7 CGCAGAG 27475 0.0 22.915201 8 GTACATG 15870 0.0 22.785645 1 TACATGG 16030 0.0 22.16979 2 GAGTACT 16235 0.0 22.103064 12-13 GCAGAGT 28355 0.0 21.50179 9 GTACTTT 17170 0.0 20.913237 14-15 ACATGGG 16285 0.0 20.450699 3 AGTACTT 16875 0.0 20.32374 12-13 TATGCGA 120 0.004818163 19.753864 5 AGAGTAC 26775 0.0 19.166132 10-11 CAGAGTA 28160 0.0 19.056795 10-11 TGGTATC 5635 0.0 18.945316 2 >>END_MODULE