FastQCFastQC Report
Fri 27 May 2016
SRR939427_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939427_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3564406
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162390.4555878314647658No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128990.3618835789188998No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90800.25474090213067757No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA55380.15536950616736703No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA136900.036.3673671
GTATCAA209450.033.1445241
GTACATG163400.026.3311651
TATCAAC266150.026.1420442
ATCAACG268250.025.7032493
TCAACGC272800.025.3424434
CAACGCA272450.025.1414665
TACATGG172550.024.9577122
AACGCAG276200.024.8687826
ACATGGG175500.023.9709013
GAGTACT157150.022.25665512-13
ACGCAGA306800.021.9425757
CGCAGAG311800.021.5874528
CATGGGA98600.021.2678134
AGAGTAC241100.020.96746310-11
GTACTTT171000.020.90012714-15
GCAGAGT304950.020.7298569
AGTACTT166500.020.50322212-13
CATGGGG77650.019.730324
GAGTACA136950.018.7987961
Produced by FastQC (version 0.11.5)