FastQCFastQC Report
Fri 27 May 2016
SRR939427_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939427_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3564406
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240280.6741095150215772No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210210.589747632564865No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154380.4331156439530177No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA83570.2344570175226952No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49330.13839613108046614No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT46980.13180316720373605No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46480.13040040893209134No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA156350.035.680071
GTATCAA232700.030.59291
GTACATG181850.026.2981341
TACATGG190500.024.7504862
ACATGGG188600.023.8139213
ATCAACG297450.023.8117583
CTACGCG1000.001679553123.688324
TCAACGC298850.023.652654
TATCAAC302300.023.5287152
CAACGCA301950.023.3784395
AACGCAG306200.023.115846
GAGTACT191400.021.37424512-13
CATGGGA109300.021.1959534
CGTACAC2509.795258E-920.845723
GTACTTT199450.020.45217314-15
ACGCAGA344400.020.4280837
AGTACTT200400.020.29611612-13
CGCAGAG347000.020.2067538
AGAGTAC286650.020.02330410-11
CATGGGG79650.019.2718544
Produced by FastQC (version 0.11.5)