##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939427_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3564406 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.989202689031497 31.0 31.0 31.0 30.0 34.0 2 31.247625270521933 31.0 31.0 34.0 30.0 34.0 3 31.233572438156596 31.0 31.0 34.0 28.0 34.0 4 35.176242268697784 35.0 35.0 37.0 33.0 37.0 5 34.798929190445755 35.0 35.0 37.0 32.0 37.0 6 34.75404653678621 35.0 35.0 37.0 32.0 37.0 7 34.622101971548695 35.0 35.0 37.0 30.0 37.0 8 34.6387319514107 35.0 35.0 37.0 30.0 37.0 9 35.98552381518828 37.0 35.0 39.0 30.0 39.0 10-11 35.84359904567549 37.0 35.0 39.0 30.0 39.0 12-13 35.80479103671131 37.0 35.0 39.0 30.0 39.0 14-15 36.65120050858404 38.0 35.5 40.0 30.5 41.0 16-17 36.66302155253919 38.0 35.5 40.0 31.0 41.0 18-19 36.63695423585304 38.0 35.0 40.0 30.0 41.0 20-21 36.539475581625666 38.0 35.0 40.0 30.0 41.0 22-23 36.392047090034076 38.0 35.0 40.0 30.0 41.0 24-25 36.157469575575846 38.0 34.0 40.0 29.5 41.0 26-27 35.781518154778105 38.0 34.0 40.0 27.5 41.0 28-29 35.4862974363751 38.0 34.0 40.0 27.0 41.0 30-31 35.24698589330171 38.0 34.0 40.0 27.0 41.0 32-33 35.00977105301696 37.0 33.0 40.0 27.0 41.0 34-35 34.92519959847447 37.5 33.0 40.0 26.0 41.0 36-37 34.89626055505461 38.0 33.0 40.0 25.5 41.0 38-39 34.85239293728043 38.0 33.0 40.0 25.0 41.0 40-41 34.622232848895436 38.0 33.0 40.0 25.0 41.0 42-43 34.29052835170853 37.0 33.0 40.0 24.0 41.0 44-45 34.087750946441005 37.0 33.0 40.0 24.0 41.0 46-47 33.729455482905145 37.0 32.5 40.0 23.0 41.0 48-49 33.39358016454916 37.0 32.0 40.0 22.0 41.0 50-51 33.112573455436895 37.0 31.5 40.0 20.5 41.0 52-53 32.73568008245974 36.0 31.0 39.0 19.0 41.0 54-55 32.50917417937238 36.0 31.0 39.0 18.5 40.0 56-57 32.09949231372632 35.5 30.5 39.0 16.5 40.0 58-59 31.65485188836513 35.0 30.0 39.0 13.5 40.0 60-61 31.35520701064918 35.0 29.0 38.5 12.5 40.0 62-63 30.96473381539589 35.0 29.0 38.0 8.0 40.0 64-65 30.526175749900545 34.0 28.5 38.0 8.0 40.0 66-67 28.848368984902393 33.5 25.0 36.5 4.5 39.5 68-69 25.23369868640104 29.5 15.5 35.0 2.0 38.0 70-71 24.63288777989937 29.5 14.0 35.0 2.0 38.0 72-73 26.429275452908563 31.0 21.0 35.0 2.0 37.5 74-75 27.031219928369552 31.5 23.5 35.0 2.0 37.0 76-77 27.06218174921712 31.5 25.0 34.5 2.0 36.0 78-79 27.895404872508912 32.5 26.0 35.0 2.0 37.0 80-81 27.75065999776681 33.0 26.0 35.0 2.0 36.5 82-83 27.349667939061938 32.5 26.0 35.0 2.0 36.0 84-85 27.013164044724423 32.0 25.5 35.0 2.0 36.0 86-87 26.578775397639888 32.0 25.0 34.5 2.0 35.0 88-89 26.100491077615736 32.0 24.0 34.0 2.0 35.0 90-91 25.653550549516524 32.0 23.0 34.0 2.0 35.0 92-93 25.095033646559905 31.0 19.5 34.0 2.0 35.0 94-95 23.73950133626753 31.0 8.0 34.0 2.0 35.0 96-97 22.938923063197628 30.0 2.0 34.0 2.0 35.0 98-99 22.61395475150698 30.0 2.0 34.0 2.0 35.0 100-101 21.372980519054224 28.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.43438495960529266 1101 2 0.3319472751230599 1101 3 0.45216391067251394 1101 4 0.32496990649631385 1101 5 0.4715744751898896 1101 6 0.6264919068894272 1101 7 0.5959203578053547 1101 8 0.5657608408223354 1101 9 0.6933422328282646 1101 10-11 0.8155408274398397 1101 12-13 0.72779304180645 1101 14-15 0.8471521840068021 1101 16-17 0.8124378178666518 1101 18-19 0.7850238848634987 1101 20-21 0.8400692090407063 1101 22-23 0.927440226869976 1101 24-25 0.8315057120364244 1101 26-27 0.8527656169357485 1101 28-29 0.8083282583599427 1101 30-31 0.7785734390518471 1101 32-33 0.7769752967489367 1101 34-35 0.9577532252091956 1101 36-37 0.8194612813683335 1101 38-39 0.7608762145824102 1101 40-41 0.9374985598738661 1101 42-43 0.845766865906775 1101 44-45 0.6469599429667383 1101 46-47 0.6330824151362506 1101 48-49 0.43290269135203374 1101 50-51 0.4568744247733818 1101 52-53 0.7451727041661513 1101 54-55 0.40027960715199384 1101 56-57 0.4793856000174941 1101 58-59 0.3513835481705847 1101 60-61 0.10018972870907916 1101 62-63 0.045091454477489634 1101 64-65 -0.1785544890687234 1101 66-67 -3.0448119445605464 1101 68-69 -7.759182607200508 1101 70-71 -11.123239733698737 1101 72-73 -11.150617688013764 1101 74-75 -9.563264056112946 1101 76-77 -4.613424278500819 1101 78-79 -1.5329091248672988 1101 80-81 -0.8175683516215635 1101 82-83 -0.5233554936921756 1101 84-85 -0.6578217621647227 1101 86-87 -0.5238796403549486 1101 88-89 -0.5805861013368059 1101 90-91 -0.5805378208574261 1101 92-93 -0.942883441243648 1101 94-95 -4.5083662447444866 1101 96-97 -1.5847092840752843 1101 98-99 -0.8993805374873283 1101 100-101 -0.6533202800549667 1102 1 0.4187753995217385 1102 2 0.31727349124123805 1102 3 0.3862030240851517 1102 4 0.2292142808943538 1102 5 0.4077220166861366 1102 6 0.5269173449921709 1102 7 0.5363496422062681 1102 8 0.5206825389473408 1102 9 0.6856246199097029 1102 10-11 0.6456278694801867 1102 12-13 0.6436353342001624 1102 14-15 0.5806760341825452 1102 16-17 0.6021101945662082 1102 18-19 0.6993952809133717 1102 20-21 0.765202422294486 1102 22-23 0.7883719222431651 1102 24-25 0.7742400391531348 1102 26-27 0.7605908792970624 1102 28-29 0.7302147894367863 1102 30-31 0.5973408724765008 1102 32-33 0.5701062147320997 1102 34-35 0.6962174577632609 1102 36-37 0.9589298511708222 1102 38-39 0.9378063214031798 1102 40-41 0.8370718709424523 1102 42-43 0.997611603670542 1102 44-45 0.6070811795359603 1102 46-47 0.7095002294382198 1102 48-49 0.6958819932645426 1102 50-51 0.6927748494297177 1102 52-53 0.8014760207383844 1102 54-55 0.7126588240081659 1102 56-57 0.6295718171363376 1102 58-59 0.5479082770534696 1102 60-61 0.36533218245969223 1102 62-63 0.49834720516232167 1102 64-65 0.4661941412738777 1102 66-67 -2.678670938644128 1102 68-69 -7.706593860906086 1102 70-71 -11.045306462281424 1102 72-73 -9.254450539778059 1102 74-75 -7.743543256708342 1102 76-77 -3.9774982900334592 1102 78-79 -1.1018184491696452 1102 80-81 -0.4024476310191609 1102 82-83 -0.2825803551629029 1102 84-85 -0.28981154914859886 1102 86-87 -0.24261869560405458 1102 88-89 -0.23844458883477415 1102 90-91 -0.32059685997083776 1102 92-93 -0.7266909949980089 1102 94-95 -4.326568069078107 1102 96-97 -1.060791485408668 1102 98-99 -0.4239034245249904 1102 100-101 -0.24916307278014926 1103 1 0.3894640091361268 1103 2 0.24872205322918717 1103 3 0.38374309382131955 1103 4 0.30731459632136193 1103 5 0.5269582824527035 1103 6 0.516477511883636 1103 7 0.5859961160873368 1103 8 0.47839035444256695 1103 9 0.7557431198858637 1103 10-11 0.77312162099593 1103 12-13 0.6371011065831951 1103 14-15 0.7782639955764665 1103 16-17 0.6866334558095986 1103 18-19 0.6204326988848905 1103 20-21 0.7610407825942929 1103 22-23 0.7211266029641621 1103 24-25 0.7782725779388784 1103 26-27 0.8378650445018536 1103 28-29 0.9397012011352004 1103 30-31 0.9640209401368551 1103 32-33 0.9604905809875106 1103 34-35 1.036299358108323 1103 36-37 0.9337026718135064 1103 38-39 1.0840858179338326 1103 40-41 1.0229663496565422 1103 42-43 1.1018120756507344 1103 44-45 0.9506451706196515 1103 46-47 0.9299898323837326 1103 48-49 0.8001339781034389 1103 50-51 0.7079167291033244 1103 52-53 0.9285428148101218 1103 54-55 0.8374732374590437 1103 56-57 0.7859632942063541 1103 58-59 0.8056572645835338 1103 60-61 0.7857191549597253 1103 62-63 0.7949071471313012 1103 64-65 0.5392898995979856 1103 66-67 -2.8001832057173885 1103 68-69 -7.270487114787212 1103 70-71 -11.013896050410617 1103 72-73 -8.901054924338379 1103 74-75 -7.263681851799678 1103 76-77 -3.6332181235806225 1103 78-79 -0.6944771718904654 1103 80-81 -0.06874835237769616 1103 82-83 0.0737345347819165 1103 84-85 -0.03520506913698895 1103 86-87 0.19328642995088785 1103 88-89 0.3810333665943588 1103 90-91 0.3327035905626623 1103 92-93 0.4137734138243587 1103 94-95 1.1737498379391411 1103 96-97 1.234345732606247 1103 98-99 0.9318159117445752 1103 100-101 0.8611611177858087 1104 1 0.34065954125155073 1104 2 0.17819968671193465 1104 3 0.3485908260743926 1104 4 0.16234497998249964 1104 5 0.4844857908203011 1104 6 0.5817124275442254 1104 7 0.544879545027456 1104 8 0.4252625009890991 1104 9 0.5769049258588765 1104 10-11 0.6830465303817661 1104 12-13 0.5895368068659437 1104 14-15 0.7286989232902386 1104 16-17 0.7656535112036238 1104 18-19 0.7430410205997262 1104 20-21 0.7691917839966109 1104 22-23 0.6318262653229141 1104 24-25 0.8973697750151146 1104 26-27 0.9370726422149929 1104 28-29 1.009743022665944 1104 30-31 1.0888243477965034 1104 32-33 0.8731888952121523 1104 34-35 0.8650058976222752 1104 36-37 0.894816447343608 1104 38-39 0.7110932058303092 1104 40-41 0.9662182028345114 1104 42-43 1.0618739508029833 1104 44-45 0.8250583689133677 1104 46-47 0.7620620542880303 1104 48-49 0.7345430375444053 1104 50-51 0.7657169345646295 1104 52-53 0.8107961257657124 1104 54-55 0.7531553492589609 1104 56-57 0.8653526368061222 1104 58-59 0.9283763692948632 1104 60-61 0.6489692887855902 1104 62-63 0.7397004665747673 1104 64-65 0.4940358368373303 1104 66-67 -2.502574466192396 1104 68-69 -6.9610077556219 1104 70-71 -8.431908878195348 1104 72-73 -7.950198609758221 1104 74-75 -6.7053219073355095 1104 76-77 -3.317004297710728 1104 78-79 -0.3313026237205001 1104 80-81 0.43202462806727127 1104 82-83 0.5806227999379168 1104 84-85 0.5535027693867285 1104 86-87 0.7304832137242556 1104 88-89 0.7656419873599809 1104 90-91 0.6174489681896134 1104 92-93 0.740862553241783 1104 94-95 1.6403219233587834 1104 96-97 1.8306395475978796 1104 98-99 1.459070839498871 1104 100-101 1.4293923020218564 1105 1 0.525067162109373 1105 2 0.4469657452822098 1105 3 0.5011086624329515 1105 4 0.38728992384329075 1105 5 0.45181128790185454 1105 6 0.5484053701378713 1105 7 0.628846068454088 1105 8 0.6149232097450437 1105 9 0.8424224688219866 1105 10-11 0.8668135563434518 1105 12-13 0.8023652368850236 1105 14-15 1.0170081315852428 1105 16-17 0.9743143719963854 1105 18-19 0.9631712218222148 1105 20-21 1.0524623894336358 1105 22-23 1.103312625993894 1105 24-25 1.1474085151638675 1105 26-27 1.2273696745988332 1105 28-29 1.3538802493619784 1105 30-31 1.2992501894270347 1105 32-33 1.313643983589266 1105 34-35 1.48137599716825 1105 36-37 1.4105189514763126 1105 38-39 1.2032247288577835 1105 40-41 1.3838955138575812 1105 42-43 1.4098066633278208 1105 44-45 1.2871652596894094 1105 46-47 1.2266702747505533 1105 48-49 1.1755558696545805 1105 50-51 1.2755590616257138 1105 52-53 1.422947108487918 1105 54-55 1.330899804364627 1105 56-57 1.4397471019035066 1105 58-59 1.4992889244844783 1105 60-61 1.5228078152692355 1105 62-63 1.6260105242928198 1105 64-65 1.5589076619248274 1105 66-67 2.153217723132588 1105 68-69 -4.77030506716363 1105 70-71 -5.148484202010874 1105 72-73 -1.2634233270282316 1105 74-75 1.2790587574975625 1105 76-77 1.5165368468061082 1105 78-79 1.3712955468037862 1105 80-81 1.4097139571171304 1105 82-83 1.5163506685394665 1105 84-85 1.475136279062422 1105 86-87 1.624226485841163 1105 88-89 1.5973367935486422 1105 90-91 1.4401619392177025 1105 92-93 1.569943686490607 1105 94-95 2.3934912425338766 1105 96-97 2.3864536144605175 1105 98-99 2.1618429116588977 1105 100-101 1.9528471580216369 1106 1 0.11846873728496377 1106 2 0.1688258555943669 1106 3 0.17898888778985267 1106 4 0.16503509990569398 1106 5 0.3232317461362655 1106 6 0.24010412951868432 1106 7 0.2722235736934877 1106 8 0.2545413697379857 1106 9 0.261856889734446 1106 10-11 0.28120825625310175 1106 12-13 0.2794182652842139 1106 14-15 0.41539492991584837 1106 16-17 0.3147497625641904 1106 18-19 0.32588365719535517 1106 20-21 0.5011919832647536 1106 22-23 0.4311396449667484 1106 24-25 0.49402085190506995 1106 26-27 0.5356854642141968 1106 28-29 0.7365911772796494 1106 30-31 0.6849990054303632 1106 32-33 0.5993734180223598 1106 34-35 0.6599686426442659 1106 36-37 0.7369276996915772 1106 38-39 0.661865746221018 1106 40-41 0.7155370617144001 1106 42-43 0.750287815936467 1106 44-45 0.7100494911476574 1106 46-47 0.6179960350429567 1106 48-49 0.520751730228028 1106 50-51 0.5070199071044144 1106 52-53 0.7345555858995851 1106 54-55 0.6524565584925526 1106 56-57 0.5834473769436244 1106 58-59 0.7075564268147225 1106 60-61 0.6633947012655419 1106 62-63 0.7294792036689017 1106 64-65 0.7135991908390977 1106 66-67 1.4242845894858291 1106 68-69 -5.252708601069937 1106 70-71 -6.107304614265331 1106 72-73 -2.173321489823298 1106 74-75 0.6018034660052223 1106 76-77 0.6959318341705938 1106 78-79 0.6418606330200483 1106 80-81 0.6349712259911051 1106 82-83 0.6702657489329233 1106 84-85 0.7248700869109435 1106 86-87 0.815796162938689 1106 88-89 0.732532539396523 1106 90-91 0.6454156842863448 1106 92-93 0.8015329412814083 1106 94-95 1.5964213783183148 1106 96-97 1.723277489119699 1106 98-99 1.3903104774278923 1106 100-101 1.2217059097651948 1107 1 0.034380326904997816 1107 2 0.009396117829133743 1107 3 0.058182923167720446 1107 4 0.05269943476726979 1107 5 0.13968215196145195 1107 6 0.20659923867835062 1107 7 0.2086744086812118 1107 8 0.16083359691319998 1107 9 0.21731238488764149 1107 10-11 0.1765844840315154 1107 12-13 0.3068717338950222 1107 14-15 0.23954847426857384 1107 16-17 0.09855932918966204 1107 18-19 0.3228392689530679 1107 20-21 0.3633415188970659 1107 22-23 0.3286598753401506 1107 24-25 0.49765533864344746 1107 26-27 0.5458909099840739 1107 28-29 0.42660717148306304 1107 30-31 0.3689693039596378 1107 32-33 0.43400734862664336 1107 34-35 0.4498762996346599 1107 36-37 0.41773203586565444 1107 38-39 0.27906246702425364 1107 40-41 0.3016388828972367 1107 42-43 0.5254454459561018 1107 44-45 0.4246459345354907 1107 46-47 0.2071073585558878 1107 48-49 0.5315337150639792 1107 50-51 0.45057318470497876 1107 52-53 0.5206615341516567 1107 54-55 0.555074311775293 1107 56-57 0.6030977226860585 1107 58-59 0.5695434796653664 1107 60-61 0.5834608410057349 1107 62-63 0.6386835488211666 1107 64-65 0.6205788021297316 1107 66-67 1.3345964636169292 1107 68-69 -5.358755290663449 1107 70-71 -5.946867726796949 1107 72-73 -2.139100154244449 1107 74-75 0.425115042157973 1107 76-77 0.4429181685689123 1107 78-79 0.5008822516881963 1107 80-81 0.4384058625282812 1107 82-83 0.5506331357654943 1107 84-85 0.7241786218020216 1107 86-87 0.7805492144522148 1107 88-89 0.6836144601411256 1107 90-91 0.5921365368927773 1107 92-93 0.7472131405444742 1107 94-95 1.6511377265610747 1107 96-97 1.7652130214020332 1107 98-99 1.6427882164311107 1107 100-101 1.6166795343700038 1108 1 0.0457506276154902 1108 2 -0.004581706014601394 1108 3 0.05182617648016574 1108 4 0.06816576588040846 1108 5 -0.0012969001843430306 1108 6 0.19265197226757635 1108 7 0.1295091736992262 1108 8 0.05719212500569171 1108 9 0.12027051786851217 1108 10-11 0.20289887064442524 1108 12-13 0.2139305342689184 1108 14-15 0.21428545016356537 1108 16-17 0.020202398356879314 1108 18-19 0.1617157295216316 1108 20-21 0.20149530951993455 1108 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0.5264349898032172 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 16.0 3 56.0 4 127.0 5 296.0 6 827.0 7 1846.0 8 4046.0 9 6446.0 10 10271.0 11 15277.0 12 19866.0 13 24536.0 14 29059.0 15 36285.0 16 42860.0 17 49035.0 18 45191.0 19 38807.0 20 37131.0 21 37856.0 22 40267.0 23 45962.0 24 55129.0 25 68255.0 26 85623.0 27 106851.0 28 128686.0 29 153967.0 30 181016.0 31 210092.0 32 243153.0 33 281612.0 34 327444.0 35 377719.0 36 393996.0 37 326573.0 38 131137.0 39 7090.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.79678105957608 20.257043799870637 15.20683467116774 22.73934046938555 2 16.100408895353993 21.857549972669226 37.917439729184 24.124601402792788 3 18.704378794110436 25.627215306000494 30.706631062791388 24.961774837097682 4 12.88750495875049 17.697759458378197 36.55021341564345 32.86452216722786 5 13.071063853664011 38.59552799910223 33.937156323644935 14.396251823588823 6 31.4678518664821 37.03668998424983 16.767281841630837 14.728176307637234 7 27.852999910784575 33.73294175803767 20.713801963076037 17.700256368101723 8 26.777954026561506 34.35183870748731 19.708220668464815 19.16198659748637 9 26.691572172193627 15.978651141312186 20.406625956751277 36.92315072974291 10-11 25.114857847282266 26.94798235666756 26.74019177388883 21.196968022161336 12-13 25.888619309921484 24.551201518569997 27.71017106356571 21.85000810794281 14-15 22.88915740799449 25.729827073571304 26.33450005414647 25.04651546428774 16-17 21.816141437648298 28.99428304503075 26.987646579821696 22.201928937499254 18-19 21.493844489077585 26.87367771051634 30.21431294813357 21.418164852272508 20-21 22.273602147679323 26.01959849966565 30.316373936691548 21.390425415963477 22-23 22.14173974569676 25.944869916614437 30.390042548463896 21.523347789224907 24-25 22.080551429887617 26.283159101404273 30.182883206907405 21.453406261800705 26-27 21.596641449199858 26.50391251585648 30.255391203190328 21.64405483175333 28-29 21.525686497892664 26.587910453624563 30.484308619596266 21.402094428886507 30-31 21.59449297734249 26.396289933727974 30.166873863189693 21.842343225739842 32-33 21.31099153323346 26.700329100254017 30.091075994676373 21.897603371836148 34-35 22.18570500259254 26.461292027770483 29.917661596062032 21.435341373574946 36-37 21.964027696062256 26.306962349699997 29.706652587413767 22.022357366823982 38-39 21.622355225741277 26.79346576799188 30.055035148043608 21.529143858223236 40-41 21.72978255077895 26.428072160339955 30.26821026453352 21.573935024347577 42-43 21.67966588836097 26.67103562588079 29.426625356739045 22.222673129019192 44-45 21.79971302162616 26.802892699322406 29.36071839232575 22.036675886725686 46-47 21.89044743610122 26.994514817462946 29.049857534655892 22.065180211779946 48-49 22.069799506260487 27.205662123051816 28.62387487925765 22.100663491430048 50-51 21.803256406180758 27.25840232887692 28.722220880473188 22.216120384469136 52-53 22.329557084124282 27.14564357444744 28.36267883931226 22.162120502116014 54-55 21.877460840612624 27.076889820313895 28.547982970456577 22.497666368616905 56-57 22.090745697961147 27.563850693136665 28.141862176466525 22.203541432435664 58-59 22.18458677698125 26.95304273993274 28.36747483808676 22.494895644999247 60-61 22.064918439853116 27.30899564053913 28.582070445801342 22.044015473806407 62-63 21.95642933795319 27.314624788704734 28.698196713262682 22.0307491600794 64-65 21.86535466495783 27.10435065370489 28.509088797193666 22.521205884143612 66-67 21.471768499200085 27.495284102618715 28.670726191145313 22.36222120703589 68-69 21.455682902264268 26.86944217314583 29.377766627909246 22.297108296680655 70-71 21.04047723690645 26.932929077607614 29.72637011338552 22.30022357210041 72-73 21.83271468495917 27.19145642070359 28.3962628170781 22.57956607725914 74-75 21.516409016765493 27.60103570385478 28.240996278439745 22.641559000939978 76-77 21.76555665188839 27.38312212051876 28.29481959588389 22.556501631708958 78-79 21.46937198566432 27.556325701657453 28.219940745450238 22.75436156722799 80-81 21.644741271925394 27.861649777239112 27.83390286054158 22.65970609029391 82-83 21.88980436010937 27.612749501599986 27.69469863982947 22.802747498461173 84-85 21.592405578937974 27.62144660288418 27.6247851675707 23.161362650607142 86-87 21.744968966038197 27.675922591773123 27.84999108545993 22.729117356728754 88-89 21.51660920457673 27.64519429611722 27.51686899737654 23.32132750192951 90-91 21.42750529699059 27.667208710407238 27.823551295518207 23.08173469708396 92-93 21.732852066586737 27.64671811232559 27.66356553457881 22.956864286508864 94-95 21.066746184990325 27.294453709397644 28.124236414612135 23.514563690999893 96-97 21.164059549629425 27.182671409895935 28.194825583406235 23.458443457068405 98-99 21.18271004410285 27.226354974351434 27.632913218004106 23.958021763541606 100-101 21.051595679761405 27.01498672195794 28.003299519802027 23.93011807847863 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 6734.0 1 4520.0 2 2458.5 3 2779.0 4 2705.5 5 2720.0 6 4145.0 7 7532.5 8 11038.0 9 12570.5 10 11256.5 11 9028.0 12 8079.0 13 7692.0 14 7540.5 15 7339.5 16 7253.5 17 7302.5 18 7391.0 19 7850.5 20 8382.5 21 9168.5 22 10496.0 23 11983.0 24 13501.5 25 15763.5 26 19215.0 27 22921.5 28 25971.0 29 30116.5 30 36898.5 31 45058.0 32 54849.5 33 63475.5 34 69893.5 35 78066.0 36 89656.0 37 99354.5 38 102092.0 39 101139.0 40 99905.0 41 101134.5 42 102487.0 43 106561.0 44 112951.5 45 116010.0 46 116582.0 47 120285.5 48 130422.0 49 133243.0 50 128588.5 51 125065.5 52 121298.5 53 120785.0 54 120285.0 55 114061.0 56 105664.0 57 96075.5 58 86495.5 59 76716.5 60 64942.5 61 52708.0 62 42013.5 63 32850.0 64 24829.5 65 18759.0 66 13910.0 67 10367.5 68 7553.5 69 5220.5 70 3631.0 71 2730.0 72 2157.5 73 1763.0 74 1330.0 75 898.0 76 597.5 77 358.5 78 226.5 79 132.5 80 80.0 81 65.5 82 40.5 83 20.5 84 14.5 85 8.5 86 6.5 87 3.5 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022752739166077042 2 0.018460298854844258 3 0.0 4 0.0 5 1.6833099259736404E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.3846890617959906E-4 18-19 0.0010941514518828662 20-21 1.4027582716447004E-5 22-23 0.0 24-25 0.0 26-27 3.226344024782811E-4 28-29 0.01868474017830741 30-31 0.004404660972964359 32-33 0.008262246219987286 34-35 0.009370425254586598 36-37 0.10130720237818025 38-39 0.10328509154119928 40-41 0.11770544657370681 42-43 0.10324300879304994 44-45 0.09848765825217441 46-47 0.0993152856324448 48-49 0.10160178161522566 50-51 0.09953972695590793 52-53 0.07740420142935457 54-55 0.06623824558706276 56-57 0.03725725969488324 58-59 0.015079651420180529 60-61 0.008935570190376742 62-63 0.002272468400064415 64-65 0.005134095274219604 66-67 0.4201401299403042 68-69 0.8062773993759409 70-71 0.8974426594501299 72-73 0.28104261972401573 74-75 0.1481453010683968 76-77 0.014153830960895026 78-79 0.008178080723688605 80-81 0.0012905376099131244 82-83 0.0 84-85 0.0 86-87 1.2624824444802305E-4 88-89 7.574894666881382E-4 90-91 0.0033105095210814927 92-93 0.0017534478395558755 94-95 0.2242449373051218 96-97 0.2294631980756401 98-99 0.013017596760862822 100-101 0.007041846523656396 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3564406.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 28.143631260167346 #Duplication Level Percentage of deduplicated Percentage of total 1 62.888548669470154 17.699121242406555 2 13.83195525282304 7.785628964851727 3 6.515702681806743 5.501266010329573 4 4.033540830763107 4.540739432553037 5 2.6304784077868533 3.7015607173292655 6 1.796659771085758 3.0338718058448544 7 1.3531920766695256 2.6658617180977053 8 1.0448832746512675 2.352544767336115 9 0.758188097879394 1.9204349627308797 >10 4.570516721652159 23.103845237835035 >50 0.31671385611827935 6.193066698169165 >100 0.23339354847421917 12.807744489767014 >500 0.018632174482021327 3.504149624561777 >1k 0.007189589066284487 3.2281962028971463 >5k 1.012618178349928E-4 0.23816407563550163 >10k+ 3.0378545350497835E-4 1.7238040496547309 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24028 0.6741095150215772 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21021 0.589747632564865 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15438 0.4331156439530177 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8357 0.2344570175226952 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4933 0.13839613108046614 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4698 0.13180316720373605 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4648 0.13040040893209134 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 2.805516543289401E-5 0.0 0.0 0.0 0.0 9 2.805516543289401E-5 0.0 0.0 0.0 0.0 10-11 2.805516543289401E-5 0.0 0.0 5.611033086578802E-5 0.0 12-13 2.805516543289401E-5 0.0 0.0 1.1222066173157604E-4 0.0 14-15 2.805516543289401E-5 0.0 0.0 1.9638615803025804E-4 0.0 16-17 2.805516543289401E-5 0.0 0.0 3.5068956791117507E-4 0.0 18-19 2.805516543289401E-5 0.0 0.0 4.909653950756452E-4 0.0 20-21 2.805516543289401E-5 0.0 0.0 7.574894666881383E-4 0.0 22-23 2.805516543289401E-5 0.0 0.0 0.0016271995951078525 0.0 24-25 2.805516543289401E-5 0.0 0.0 0.002651213133408484 0.0 26-27 2.805516543289401E-5 0.0 0.0 0.003534950844544645 0.0 28-29 2.805516543289401E-5 0.0 0.0 0.005863529575474848 0.0 30-31 2.805516543289401E-5 0.0 0.0 0.012765100271966773 0.0 32-33 2.805516543289401E-5 0.0 0.0 0.024464104257483577 0.0 34-35 2.805516543289401E-5 0.0 0.0 0.04209677573205746 0.0 36-37 2.805516543289401E-5 0.0 0.0 0.06888945872047124 0.0 38-39 2.805516543289401E-5 0.0 0.0 0.11052332422288594 0.0 40-41 2.805516543289401E-5 0.0 0.0 0.15877820876746365 0.0 42-43 2.805516543289401E-5 0.0 0.0 0.20867432048986562 0.0 44-45 2.805516543289401E-5 0.0 0.0 0.2688526503434233 0.0 46-47 2.805516543289401E-5 0.0 0.0 0.3431988387405924 0.0 48-49 2.805516543289401E-5 0.0 0.0 0.4241379910144916 0.0 50-51 2.805516543289401E-5 0.0 0.0 0.5113755279280755 0.0 52-53 2.805516543289401E-5 0.0 0.0 0.595653244888489 0.0 54-55 2.805516543289401E-5 0.0 0.0 0.6891190285281755 0.0 56-57 2.805516543289401E-5 0.0 0.0 0.7944240919805432 0.0 58-59 2.805516543289401E-5 0.0 0.0 0.9014124653588844 0.0 60-61 2.805516543289401E-5 0.0 0.0 1.0055392118630706 0.0 62-63 2.805516543289401E-5 2.805516543289401E-5 0.0 1.111405378624096 0.0 64-65 2.805516543289401E-5 2.805516543289401E-5 0.0 1.2220268959259972 0.0 66-67 2.805516543289401E-5 2.805516543289401E-5 0.0 1.3403215009737948 0.0 68-69 2.805516543289401E-5 2.805516543289401E-5 0.0 1.48132676243952 0.0 70-71 2.805516543289401E-5 2.805516543289401E-5 0.0 1.6687212399485356 0.0 72-73 2.805516543289401E-5 2.805516543289401E-5 0.0 1.8693437279591607 0.0 74-75 2.805516543289401E-5 2.805516543289401E-5 0.0 2.088300266580182 0.0 76-77 2.805516543289401E-5 2.805516543289401E-5 0.0 2.3355504395402766 0.0 78-79 2.805516543289401E-5 2.805516543289401E-5 0.0 2.6164527834371283 0.0 80-81 2.805516543289401E-5 2.805516543289401E-5 0.0 2.9221138108285083 0.0 82-83 2.805516543289401E-5 2.805516543289401E-5 0.0 3.232431995681749 0.0 84-85 2.805516543289401E-5 2.805516543289401E-5 0.0 3.5496517512314814 0.0 86-87 2.805516543289401E-5 2.805516543289401E-5 0.0 3.876845118092608 0.0 88-89 2.805516543289401E-5 2.805516543289401E-5 0.0 4.22979312682113 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 15635 0.0 35.68007 1 GTATCAA 23270 0.0 30.5929 1 GTACATG 18185 0.0 26.298134 1 TACATGG 19050 0.0 24.750486 2 ACATGGG 18860 0.0 23.813921 3 ATCAACG 29745 0.0 23.811758 3 CTACGCG 100 0.0016795531 23.68832 4 TCAACGC 29885 0.0 23.65265 4 TATCAAC 30230 0.0 23.528715 2 CAACGCA 30195 0.0 23.378439 5 AACGCAG 30620 0.0 23.11584 6 GAGTACT 19140 0.0 21.374245 12-13 CATGGGA 10930 0.0 21.195953 4 CGTACAC 250 9.795258E-9 20.84572 3 GTACTTT 19945 0.0 20.452173 14-15 ACGCAGA 34440 0.0 20.428083 7 AGTACTT 20040 0.0 20.296116 12-13 CGCAGAG 34700 0.0 20.206753 8 AGAGTAC 28665 0.0 20.023304 10-11 CATGGGG 7965 0.0 19.271854 4 >>END_MODULE