FastQCFastQC Report
Fri 27 May 2016
SRR939426_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939426_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3554649
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162400.45686648667702495No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119550.3363201261221572No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92710.2608133742600183No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA55360.1557397087588676No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA136300.034.3428761
GTATCAA206100.034.0679321
GTACATG166500.029.1415351
TACATGG173900.027.6756082
TATCAAC263900.026.4105282
ACATGGG177800.026.3058153
ATCAACG270400.026.0170443
TCAACGC275500.025.7152214
CAACGCA275850.025.3431855
AACGCAG279000.024.7575026
CATGGGA101950.023.0075784
CATGGGG77950.022.8743364
ACGCAGA306300.021.8702627
GAGTACT156200.021.83967412-13
CGCAGAG311650.021.5729588
AGTACTT165300.021.0009612-13
AGAGTAC239700.020.88304710-11
GTACTTT167900.020.71317914-15
GCAGAGT303250.020.6506029
GTATAGG12250.020.5439281
Produced by FastQC (version 0.11.5)