FastQCFastQC Report
Fri 27 May 2016
SRR939426_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939426_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3554649
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT234160.6587429588687941No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT203200.57164575180278No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153180.4309286233324303No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA84530.23780125688921747No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48610.13675049210203316No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT45200.1271574211687286No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43410.12212176223306435No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA154350.035.7045251
GTATCAA232100.031.4337641
GTACATG178800.025.379791
ATCAACG294700.024.6019543
TCAACGC296150.024.4532434
TATCAAC297400.024.4472392
CAACGCA300200.024.1167375
TACATGG185000.024.1139372
AACGCAG303800.023.7841386
ACATGGG183150.023.4977743
CATGGGA104200.022.1341194
GAGTACT187400.021.76759312-13
ACGCAGA340600.021.1865487
GTACTTT193350.021.08547614-15
CGCAGAG345000.020.9163468
AGAGTAC282400.020.4140210-11
AGTACTT194350.020.40377412-13
GCAGAGT352850.019.6985429
GTATAGG11800.019.3088721
TAAGGTG11450.019.04775
Produced by FastQC (version 0.11.5)