##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939426_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3554649 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.88729829583737 31.0 31.0 31.0 28.0 34.0 2 31.138239246687927 31.0 31.0 34.0 28.0 34.0 3 31.134758171622572 31.0 31.0 34.0 28.0 34.0 4 35.05418987922577 35.0 35.0 37.0 32.0 37.0 5 34.62667115656145 35.0 35.0 37.0 31.0 37.0 6 34.68782374856139 35.0 35.0 37.0 31.0 37.0 7 34.51876514390028 35.0 35.0 37.0 30.0 37.0 8 34.416082150445796 35.0 35.0 37.0 30.0 37.0 9 35.915661996444655 37.0 35.0 39.0 30.0 39.0 10-11 35.7136298126763 37.0 35.0 39.0 30.0 39.0 12-13 35.69857572435422 37.0 35.0 39.0 30.0 39.0 14-15 36.50350934789905 38.0 35.0 40.0 30.0 41.0 16-17 36.425365345495436 38.0 35.0 40.0 30.0 41.0 18-19 36.44245099867807 38.0 35.0 40.0 30.0 41.0 20-21 36.326122916777436 38.0 34.5 40.0 29.5 41.0 22-23 36.153519236357795 38.0 34.0 40.0 29.0 41.0 24-25 35.97412627800944 38.0 34.0 40.0 29.0 41.0 26-27 35.50192297467345 38.0 34.0 40.0 27.0 41.0 28-29 35.27810804948675 38.0 33.5 40.0 27.0 41.0 30-31 34.954560773792295 37.0 33.0 40.0 26.5 41.0 32-33 34.67694320873875 37.0 33.0 40.0 25.5 41.0 34-35 34.887020631291584 37.0 33.0 40.0 26.0 41.0 36-37 34.70188814704349 37.0 33.0 40.0 25.0 41.0 38-39 34.505528253281824 37.0 33.0 40.0 25.0 41.0 40-41 34.32979107079208 37.0 33.0 40.0 24.5 41.0 42-43 34.135906667578155 37.0 33.0 40.0 24.0 41.0 44-45 33.72359816679509 37.0 32.5 40.0 23.5 41.0 46-47 33.28135225165691 37.0 31.0 40.0 22.0 41.0 48-49 32.86267983139826 36.5 31.0 39.0 19.5 41.0 50-51 32.60830042572417 36.0 31.0 39.0 18.5 40.0 52-53 32.48903337572852 36.0 31.0 39.0 18.5 40.0 54-55 32.141213520659846 36.0 30.5 39.0 16.5 40.0 56-57 31.620025493375014 35.0 29.5 39.0 13.5 40.0 58-59 31.143916459824865 35.0 29.0 38.0 11.0 40.0 60-61 30.761451693261414 35.0 28.0 38.0 8.0 40.0 62-63 30.596482521903006 34.0 28.0 38.0 8.0 40.0 64-65 30.12707386861544 34.0 28.0 38.0 7.0 40.0 66-67 29.262376819764764 33.5 27.0 37.0 4.0 39.5 68-69 25.874537542243974 31.0 17.0 35.0 2.0 39.0 70-71 24.58512317249889 29.5 12.0 35.0 2.0 38.0 72-73 25.57661994194082 30.0 18.5 35.0 2.0 37.5 74-75 26.302361077000853 31.0 21.5 35.0 2.0 37.0 76-77 25.952134795868734 30.5 22.0 34.0 2.0 36.0 78-79 27.057005628403818 32.0 25.0 35.0 2.0 36.0 80-81 27.06968972182626 32.0 25.0 35.0 2.0 36.0 82-83 26.748993079204162 32.0 25.0 35.0 2.0 36.0 84-85 26.386878141836227 32.0 24.5 34.5 2.0 35.5 86-87 25.961930559107245 32.0 23.5 34.0 2.0 35.0 88-89 25.487246279449813 31.0 21.5 34.0 2.0 35.0 90-91 24.899832726100385 31.0 19.5 34.0 2.0 35.0 92-93 24.32856957184802 31.0 17.5 34.0 2.0 35.0 94-95 23.49279830441768 30.0 7.0 34.0 2.0 35.0 96-97 22.82568377355964 30.0 2.0 34.0 2.0 35.0 98-99 21.988825760293068 29.5 2.0 34.0 2.0 35.0 100-101 20.590791945983977 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.32634333334492993 1101 2 0.18096846647135933 1101 3 0.33980349603317705 1101 4 0.33227902531927356 1101 5 0.44010898744072335 1101 6 0.3755176462330283 1101 7 0.48637824730818835 1101 8 0.6609518709284359 1101 9 0.6362704450060903 1101 10-11 0.694163434007379 1101 12-13 0.6739260659570547 1101 14-15 0.6757540293167139 1101 16-17 0.6935802462313632 1101 18-19 0.5543053781778582 1101 20-21 0.7886154061550101 1101 22-23 0.7290702387320067 1101 24-25 0.6681133583413654 1101 26-27 0.7027947813379498 1101 28-29 0.6982367015714956 1101 30-31 0.5060842564865808 1101 32-33 0.8517617155004586 1101 34-35 0.7713938551144892 1101 36-37 0.9074973903940204 1101 38-39 0.9081056841709554 1101 40-41 0.8390978423571838 1101 42-43 0.4681663355285579 1101 44-45 0.599993187999786 1101 46-47 0.5294465992040571 1101 48-49 0.6488754122250739 1101 50-51 0.4087918181481669 1101 52-53 0.11121978991661763 1101 54-55 0.1421639211813357 1101 56-57 0.26819273517409314 1101 58-59 0.3175773835974596 1101 60-61 0.2744942261574437 1101 62-63 0.058012245878391155 1101 64-65 -0.14734165882529737 1101 66-67 -0.1649866233087458 1101 68-69 -7.180428107851551 1101 70-71 -7.977489035281629 1101 72-73 -4.818023101863513 1101 74-75 -2.8945577269514438 1101 76-77 -0.3658565331391195 1101 78-79 -0.21395391394796448 1101 80-81 -0.3051257354788497 1101 82-83 -0.5101404769295605 1101 84-85 -0.5018338819560242 1101 86-87 -0.6485852032402661 1101 88-89 -0.5177463251007275 1101 90-91 -0.7035924541698471 1101 92-93 -0.6660578956783922 1101 94-95 -0.7684546941248627 1101 96-97 -0.7515037503870623 1101 98-99 -0.7205597764598544 1101 100-101 -0.6777915606084797 1102 1 0.28750810732196896 1102 2 0.31659245701943206 1102 3 0.2831473192871776 1102 4 0.33695039176367914 1102 5 0.4111692052414284 1102 6 0.3519060288684628 1102 7 0.3781604837823167 1102 8 0.5375286976829159 1102 9 0.6077475363391045 1102 10-11 0.6403412182617103 1102 12-13 0.6077724653428405 1102 14-15 0.7707063048910712 1102 16-17 0.6846086604046491 1102 18-19 0.5430636056693032 1102 20-21 0.5897579918288116 1102 22-23 0.6239724475856505 1102 24-25 0.5034081183039092 1102 26-27 0.6845937150110615 1102 28-29 0.5741165678891704 1102 30-31 0.3979370302143366 1102 32-33 0.718247263448248 1102 34-35 0.5323190119134082 1102 36-37 0.7527964277618153 1102 38-39 0.9041391665522838 1102 40-41 0.6834569176311405 1102 42-43 0.6300232125278242 1102 44-45 0.7412319112041459 1102 46-47 0.792742756949842 1102 48-49 0.8994317891332173 1102 50-51 0.6562754548146046 1102 52-53 0.24309440629869528 1102 54-55 0.06495351604939259 1102 56-57 0.3590958778034974 1102 58-59 0.26827973170571084 1102 60-61 0.3938172374193165 1102 62-63 0.1899703613481698 1102 64-65 0.1013670675527436 1102 66-67 -0.010243894766734485 1102 68-69 -6.843185832319094 1102 70-71 -7.602925646152183 1102 72-73 -1.5421288596935092 1102 74-75 0.12383969806892736 1102 76-77 0.49606589277155777 1102 78-79 0.3327419116082666 1102 80-81 0.1219261424945195 1102 82-83 0.043152693048003954 1102 84-85 -0.07571643438467746 1102 86-87 -0.10247796278593668 1102 88-89 -0.0820951212474732 1102 90-91 -0.11865267170869842 1102 92-93 -0.032487504801846256 1102 94-95 0.030152903761859307 1102 96-97 -0.2781824781058013 1102 98-99 -0.3769318009988325 1102 100-101 -0.3713552767951107 1103 1 0.3543484063782252 1103 2 0.3027948102489937 1103 3 0.4315126214075349 1103 4 0.3412477934060405 1103 5 0.4402013068061663 1103 6 0.3730845842469819 1103 7 0.43504628473831986 1103 8 0.6550653136897822 1103 9 0.49680333361818185 1103 10-11 0.5893092545815222 1103 12-13 0.6084524404214875 1103 14-15 0.7408590247913835 1103 16-17 0.718238062496404 1103 18-19 0.7231129887636385 1103 20-21 0.5325186702207745 1103 22-23 0.6016087234772982 1103 24-25 0.7036149451209255 1103 26-27 0.7222794761337497 1103 28-29 0.6238230525482393 1103 30-31 0.6673150432469797 1103 32-33 0.8185900597468176 1103 34-35 0.6432789181519141 1103 36-37 0.9285965411335511 1103 38-39 1.0728539293908668 1103 40-41 0.9199056756488702 1103 42-43 0.5121169893387076 1103 44-45 0.7097540186928057 1103 46-47 0.8185422319672071 1103 48-49 0.8569763718026664 1103 50-51 0.6848903279608223 1103 52-53 0.6408693582944451 1103 54-55 0.5611789266389309 1103 56-57 0.7726727816315808 1103 58-59 0.8040939654761914 1103 60-61 0.8189363543454107 1103 62-63 0.5463782902201721 1103 64-65 0.5111637644518723 1103 66-67 0.37481771919739515 1103 68-69 -6.42741452149669 1103 70-71 -7.716180819376028 1103 72-73 -0.969893572485109 1103 74-75 0.40826691545668226 1103 76-77 0.6623464827131365 1103 78-79 0.45384271542409493 1103 80-81 0.2679087230803745 1103 82-83 0.2975910433360305 1103 84-85 0.10338141768052367 1103 86-87 0.10969634881270807 1103 88-89 0.07160082290418046 1103 90-91 -0.27814487357167295 1103 92-93 -0.14901781897433608 1103 94-95 0.10015714243489882 1103 96-97 0.13547779480346378 1103 98-99 0.2368109472954849 1103 100-101 0.3591782342560812 1104 1 0.21825723624182913 1104 2 0.2622174263475969 1104 3 0.17275631472320896 1104 4 0.17335602065900702 1104 5 0.41481557533189317 1104 6 0.4422642350159194 1104 7 0.44225081847339 1104 8 0.46015439086640697 1104 9 0.5283488883536265 1104 10-11 0.4781390929079663 1104 12-13 0.2994923987645137 1104 14-15 0.4858740900573508 1104 16-17 0.4868185150610458 1104 18-19 0.37639233822725515 1104 20-21 0.492329671909296 1104 22-23 0.5769042150195176 1104 24-25 0.42882954932908746 1104 26-27 0.71776760591748 1104 28-29 0.5117267308997455 1104 30-31 0.4514683298004698 1104 32-33 0.5613431865478233 1104 34-35 0.37790855527111233 1104 36-37 0.7705699898661464 1104 38-39 0.5627693010176174 1104 40-41 0.49350265956240236 1104 42-43 0.5150797407213759 1104 44-45 0.5313974471024565 1104 46-47 0.7047447485743916 1104 48-49 0.6732285330111196 1104 50-51 0.5942984096165844 1104 52-53 0.5286359073374527 1104 54-55 0.5521441905844071 1104 56-57 0.5827071081939685 1104 58-59 0.7291107748752985 1104 60-61 0.7058425907588664 1104 62-63 0.47049522802792154 1104 64-65 0.3884968584935109 1104 66-67 0.2613733595723069 1104 68-69 -6.535848669095852 1104 70-71 -4.068218674648364 1104 72-73 0.2624820885160766 1104 74-75 0.8203643745832956 1104 76-77 0.9790082624367038 1104 78-79 0.5959075415668806 1104 80-81 0.4846275030971583 1104 82-83 0.4159381864965006 1104 84-85 0.5750425401945023 1104 86-87 0.4209704001559409 1104 88-89 0.373172817686946 1104 90-91 0.35120534836294937 1104 92-93 0.31754664317544723 1104 94-95 0.512219592512249 1104 96-97 0.39825521308197764 1104 98-99 0.5084020517109202 1104 100-101 0.6161546404318266 1105 1 0.12165198636802543 1105 2 0.22366544306983727 1105 3 0.21693214710264286 1105 4 0.30000104984471676 1105 5 0.45012015901775015 1105 6 0.3140897108390561 1105 7 0.31828796005077464 1105 8 0.35500316549296684 1105 9 0.563540604018641 1105 10-11 0.45994112417685074 1105 12-13 0.3663618131305668 1105 14-15 0.45465497323628057 1105 16-17 0.41338986897960694 1105 18-19 0.5252265982040782 1105 20-21 0.5446182265587467 1105 22-23 0.438304062869868 1105 24-25 0.540828397542036 1105 26-27 0.6557786225472952 1105 28-29 0.5765925157146441 1105 30-31 0.5288295821200464 1105 32-33 0.6286831805755924 1105 34-35 0.6382293137442119 1105 36-37 0.7685356993171979 1105 38-39 0.7412198630470357 1105 40-41 0.7752305712018526 1105 42-43 0.7490578141086957 1105 44-45 0.76315993012782 1105 46-47 0.8829504669837789 1105 48-49 0.9415002658818423 1105 50-51 0.7251804332068943 1105 52-53 0.62576817088339 1105 54-55 0.5945208000646147 1105 56-57 0.6559095738714298 1105 58-59 0.8291193955036604 1105 60-61 0.8193847091938551 1105 62-63 0.41015927236461636 1105 64-65 0.40849938247129813 1105 66-67 0.5467014944593664 1105 68-69 -2.933939955329155 1105 70-71 -3.0727333954821994 1105 72-73 0.6846155962764193 1105 74-75 1.1742936036680547 1105 76-77 1.1589296636354156 1105 78-79 0.8563727177831915 1105 80-81 0.65175082607389 1105 82-83 0.6566816757002734 1105 84-85 0.5957364918905483 1105 86-87 0.5655432258024007 1105 88-89 0.7267563329125757 1105 90-91 0.7046502225550988 1105 92-93 0.5452076622936914 1105 94-95 0.7089712153233201 1105 96-97 0.7396205428698934 1105 98-99 0.6101850109504596 1105 100-101 0.7156451346337889 1106 1 0.1299898518950151 1106 2 0.20977958135503272 1106 3 0.19001669984452718 1106 4 0.12485524000768322 1106 5 0.29429048999297436 1106 6 0.2128905386626485 1106 7 0.26890268486522473 1106 8 0.3491702579630811 1106 9 0.26246693676630173 1106 10-11 0.32768417398505534 1106 12-13 0.201368267008597 1106 14-15 0.3823658204806222 1106 16-17 0.36268471019518245 1106 18-19 0.3568204773266146 1106 20-21 0.36594816494490345 1106 22-23 0.2636691529157318 1106 24-25 0.45562422778679235 1106 26-27 0.5736772491126985 1106 28-29 0.35141096666054494 1106 30-31 0.3310917935975084 1106 32-33 0.5713710215824719 1106 34-35 0.5510021063945558 1106 36-37 0.5585414540893652 1106 38-39 0.5228910293509514 1106 40-41 0.49642830918313763 1106 42-43 0.4726920131338588 1106 44-45 0.5437931041404127 1106 46-47 0.5778435757533416 1106 48-49 0.7508006356882859 1106 50-51 0.6949424981805592 1106 52-53 0.39414771545704497 1106 54-55 0.41339699252845463 1106 56-57 0.5551458649708856 1106 58-59 0.7505727954713812 1106 60-61 0.8901964225069889 1106 62-63 0.4411736775437589 1106 64-65 0.49107541537451027 1106 66-67 0.6299826732957889 1106 68-69 -2.9010001175011517 1106 70-71 -3.133220703112425 1106 72-73 0.89174929050602 1106 74-75 1.4066104662043095 1106 76-77 1.4673653397273334 1106 78-79 1.1306621486390505 1106 80-81 0.9020126694926809 1106 82-83 0.7562537560851119 1106 84-85 0.5915993658736305 1106 86-87 0.7121997006022021 1106 88-89 0.808656135106741 1106 90-91 0.8274697576291352 1106 92-93 0.6513345027155815 1106 94-95 0.9542009101533289 1106 96-97 0.867514249991828 1106 98-99 0.9187103884933272 1106 100-101 0.9035001493304371 1107 1 0.052878841598030135 1107 2 0.04574547722262068 1107 3 0.07827777947803582 1107 4 -0.09120739988532733 1107 5 0.20955836063772182 1107 6 0.07415153035191224 1107 7 -0.04471733060009342 1107 8 0.05094598084863833 1107 9 0.2784892778204977 1107 10-11 0.021491558941661992 1107 12-13 0.10395185693533193 1107 14-15 -0.1098688774899017 1107 16-17 -0.09453115483829322 1107 18-19 0.08276558296159209 1107 20-21 0.10149936886936217 1107 22-23 0.08758997253459455 1107 24-25 0.0052536018061388745 1107 26-27 0.3582764700554364 1107 28-29 0.15077898809749257 1107 30-31 0.08144162666432919 1107 32-33 0.007171593005530497 1107 34-35 0.04661709402338232 1107 36-37 0.2001822871358101 1107 38-39 0.2906290874349011 1107 40-41 0.22487754658860837 1107 42-43 0.032847749511340396 1107 44-45 0.2686322037200952 1107 46-47 0.25821439478233543 1107 48-49 0.15754140073881473 1107 50-51 0.2555057140010817 1107 52-53 0.023260315185275715 1107 54-55 0.09416838684146 1107 56-57 0.3937733476528358 1107 58-59 0.4429014838474039 1107 60-61 0.4203537890703757 1107 62-63 0.21308365749890612 1107 64-65 0.16152589633297154 1107 66-67 0.4196459943276558 1107 68-69 -2.766370491446491 1107 70-71 -2.8106948587672953 1107 72-73 1.1070195608487552 1107 74-75 1.2431740075257665 1107 76-77 0.9166643463864297 1107 78-79 0.6680774620905474 1107 80-81 0.5231512336850024 1107 82-83 0.5996434327184339 1107 84-85 0.5533604465796316 1107 86-87 0.3823967385973326 1107 88-89 0.3373423795552597 1107 90-91 0.3151910794954027 1107 92-93 0.4410279205182839 1107 94-95 0.6272486955136181 1107 96-97 0.5978361902562384 1107 98-99 0.641893495370244 1107 100-101 0.7806073254730954 1108 1 -0.0549969405931634 1108 2 -0.004744198856982962 1108 3 -0.030093041598693304 1108 4 -0.012766364109531025 1108 5 -0.023687783850796507 1108 6 -0.17368276102074987 1108 7 0.10534290582007344 1108 8 0.13035965662653837 1108 9 0.12238789241743575 1108 10-11 0.2605839922646851 1108 12-13 0.12659324298885366 1108 14-15 0.16536509654304865 1108 16-17 0.31677806870352754 1108 18-19 0.217907427448516 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 51.0 4 148.0 5 364.0 6 843.0 7 2155.0 8 4501.0 9 7386.0 10 11186.0 11 16290.0 12 21158.0 13 26105.0 14 31667.0 15 38998.0 16 45914.0 17 51302.0 18 47435.0 19 41736.0 20 41695.0 21 42741.0 22 44792.0 23 49929.0 24 60096.0 25 72227.0 26 90063.0 27 111801.0 28 135414.0 29 160953.0 30 186528.0 31 215583.0 32 248758.0 33 286657.0 34 330651.0 35 364887.0 36 362023.0 37 286131.0 38 110863.0 39 5610.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.628056305752416 20.409625305510986 15.265954243838149 22.69636414489845 2 16.10092686617601 21.982859405084778 37.865667009658644 24.050546719080568 3 18.689496215221094 25.75483882583659 30.742892444874055 24.81277251406826 4 12.820309215618861 17.75848383146462 36.642476022653774 32.778730930262746 5 13.054594138549264 38.663845572375784 33.92675338690262 14.354806902172337 6 31.371086146620947 37.09378900701588 16.811983405393892 14.723141440969279 7 27.805023787158735 33.74901431899465 20.723536979319196 17.722424914527426 8 26.77943729465272 34.37436438872024 19.709625338535535 19.136572978091507 9 26.639451602675816 16.01063846247548 20.466521448390544 36.88338848645816 10-11 25.083158435089437 27.02306940544726 26.741431533509775 21.152340625953535 12-13 25.802786716775692 24.598462464226426 27.76772052599286 21.831030293005018 14-15 22.82661382319323 25.775442244789854 26.356343481452036 25.041600450564882 16-17 21.823901122705387 29.05686614745622 26.955830539792068 22.163402190046327 18-19 21.48031461949906 26.878662639258007 30.237621612071873 21.40340112917106 20-21 22.30331321039011 26.069929829921325 30.270302356153874 21.356454603534694 22-23 22.158510602534065 25.980692026813923 30.360481797692625 21.500315572959387 24-25 22.01927933942331 26.329778292801258 30.19598509564062 21.454957272134813 26-27 21.589911639066067 26.53827023231318 30.212600210661826 21.65921791795892 28-29 21.55620969927878 26.615647394808622 30.4168157104398 21.4113271954728 30-31 21.594001875574335 26.436877161696483 30.143647276120017 21.82547368660917 32-33 21.338935793299385 26.73336654823893 30.04222100252938 21.885476655932308 34-35 22.152675864003353 26.535193619096237 29.892572703077064 21.41955781382335 36-37 21.948448481988446 26.29270173000326 29.75817064867942 22.00067913932887 38-39 21.66568153229274 26.814968110087996 30.052662214178444 21.46668814344082 40-41 21.71316692794205 26.474164707101284 30.251156594050126 21.561511770906534 42-43 21.616007613287778 26.736098499726186 29.44955704123536 22.19833684575068 44-45 21.80221132296465 26.778208867816154 29.370628878402577 22.04895093081662 46-47 21.949422400662204 27.01582591215084 29.029512046106586 22.00523964108036 48-49 22.07963797032298 27.19038765486459 28.66276611258979 22.06720826222264 50-51 21.783122469543017 27.266730612275925 28.752719964121386 22.19742695405967 52-53 22.289608872500036 27.19207329946095 28.374758975946857 22.14355885209216 54-55 21.86377360853788 27.131271949668896 28.561874933592467 22.443079508200757 56-57 22.06665764308313 27.56307527103815 28.17761226411935 22.192654821759366 58-59 22.189662304187923 26.974539857447965 28.35112654377439 22.48467129458972 60-61 22.04065388236853 27.3083209920415 28.662437496931382 21.988587628658582 62-63 21.899105977223847 27.329397253071168 28.731894867749137 22.039601901955844 64-65 21.816268697762844 27.143000827846592 28.523809557302528 22.516920917088036 66-67 21.681948720766336 27.6110616854003 28.32334135649979 22.383648237333574 68-69 21.807317984744728 26.839774891397084 29.089344970915022 22.263562152943166 70-71 21.305867476569443 26.8995009437214 29.497760852299404 22.296870727409754 72-73 21.82364593103129 27.143237885076992 28.47752963477222 22.555586549119496 74-75 21.764658693320797 27.59175493361331 27.9745093127141 22.669077060351793 76-77 21.178372899376694 27.442521162320233 28.790917037165237 22.588188901137837 78-79 21.413373714796354 27.596011486432875 28.228209850803548 22.762404947967223 80-81 21.611143128307152 27.800246081496105 27.875010180718117 22.713600609478625 82-83 21.858597373061823 27.630788828374094 27.671088783826114 22.83952501473797 84-85 21.561800694050497 27.629784221498632 27.63135962694399 23.177055457506885 86-87 21.712894272356895 27.73272330448609 27.832960012783488 22.721422410373528 88-89 21.471554537729677 27.671402595287198 27.514424474745724 23.342618392237405 90-91 21.42023487244627 27.703385795259926 27.785366786867005 23.091012545426796 92-93 21.731845068540363 27.644307527834634 27.64027036341513 22.983577040209877 94-95 21.27509041919059 27.270875525788313 27.909894582618993 23.544139472402104 96-97 21.464095969974768 27.184546213383808 27.862033311212915 23.489324505428506 98-99 21.194298952046758 27.23613464210605 27.635685191700837 23.93388121414635 100-101 21.024008832542542 27.000971686597236 28.012836898599218 23.962182582261 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 6708.0 1 4478.0 2 2471.5 3 2852.5 4 2687.5 5 2640.0 6 4142.0 7 7530.5 8 10964.5 9 12505.5 10 11236.0 11 8904.5 12 7839.0 13 7508.0 14 7412.5 15 7308.0 16 7198.5 17 7218.0 18 7390.0 19 7845.5 20 8422.5 21 9274.5 22 10537.0 23 12027.0 24 13452.5 25 15682.0 26 19251.0 27 22904.5 28 26001.5 29 30419.5 30 37072.5 31 45072.0 32 54685.5 33 63372.0 34 69789.0 35 77847.0 36 89662.0 37 99318.5 38 102442.0 39 101367.5 40 99665.0 41 100697.5 42 101933.0 43 106485.5 44 113146.5 45 115760.0 46 115635.0 47 119456.5 48 130292.0 49 133546.0 50 128773.0 51 124621.5 52 119825.5 53 119170.0 54 119344.0 55 112831.5 56 104751.0 57 95623.0 58 85945.0 59 76429.5 60 64807.5 61 52688.0 62 42182.5 63 33227.0 64 25187.0 65 18755.5 66 13855.5 67 10471.5 68 7604.0 69 5230.0 70 3735.0 71 2911.0 72 2266.0 73 1797.5 74 1354.0 75 897.5 76 597.5 77 371.5 78 241.0 79 159.0 80 95.5 81 70.0 82 52.0 83 36.0 84 18.5 85 11.0 86 6.0 87 2.0 88 0.5 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02157737655672895 2 0.01772326887971217 3 4.501147651990393E-4 4 0.09216099817450331 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.012364089956561113 12-13 0.0 14-15 0.0 16-17 5.907756293237392E-4 18-19 1.6879303694963975E-4 20-21 0.0 22-23 9.846260488728985E-5 24-25 0.003333662479755385 26-27 0.0038541076770167745 28-29 0.0012518816907098281 30-31 1.2659477771222982E-4 32-33 5.626434564987992E-4 34-35 0.0029679442330311657 36-37 0.08706907489318917 38-39 0.09919404138073828 40-41 0.07992350299565443 42-43 0.08338376025312204 44-45 0.08239913420424914 46-47 0.08099252556300214 48-49 0.07525356230671439 50-51 0.07516916578823957 52-53 0.05904943075954898 54-55 0.049976805023505835 56-57 0.028075908479290076 58-59 0.024165536456623424 60-61 0.014727192473856069 62-63 0.002742886850431646 64-65 0.0069345806013477 66-67 0.1561757574376542 68-69 0.7175954644185685 70-71 0.8748683765963954 72-73 0.3571379340126128 74-75 0.05567357002055617 76-77 0.1955748654789826 78-79 0.015275769843942397 80-81 0.003811909417779364 82-83 0.0015332034189592277 84-85 2.3912346901198967E-4 86-87 0.0017723268879712177 88-89 3.7978433313668944E-4 90-91 0.004684006775352503 92-93 0.004894998071539552 94-95 0.07653357617024915 96-97 0.038569208942992685 98-99 0.01492411768363065 100-101 0.014614663782556308 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3554649.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.012231389190852 #Duplication Level Percentage of deduplicated Percentage of total 1 63.770623729269325 18.501280914665863 2 13.504812835647842 7.836095097110595 3 6.512512840616402 5.66827588371119 4 3.8827416593863404 4.505879977862694 5 2.538181904877799 3.681916036608595 6 1.7953928589180577 3.1253011834458935 7 1.31559294467816 2.6717800847492814 8 1.0007730039779088 2.322772636757016 9 0.7675793219814333 2.004227000899521 >10 4.363720983632941 22.636697766473947 >50 0.3020053410168385 6.095417802207343 >100 0.22131365653186003 12.522889678631314 >500 0.017592605403118935 3.3993265920813607 >1k 0.006758741058638721 3.0814939964301584 >5k 9.939325086233414E-5 0.24375239796956943 >10k+ 2.981797525870024E-4 1.7028929503957118 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23416 0.6587429588687941 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20320 0.57164575180278 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15318 0.4309286233324303 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8453 0.23780125688921747 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4861 0.13675049210203316 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4520 0.1271574211687286 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4341 0.12212176223306435 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 2.8132172824939958E-5 0.0 10-11 0.0 0.0 0.0 4.219825923740994E-5 1.4066086412469979E-5 12-13 0.0 0.0 0.0 5.6264345649879916E-5 2.8132172824939958E-5 14-15 0.0 0.0 0.0 7.03304320623499E-5 2.8132172824939958E-5 16-17 0.0 0.0 0.0 3.375860738992795E-4 2.8132172824939958E-5 18-19 0.0 0.0 0.0 5.485773700863292E-4 5.6264345649879916E-5 20-21 0.0 0.0 0.0 8.439651847481987E-4 5.6264345649879916E-5 22-23 0.0 0.0 0.0 0.001491005159721818 5.6264345649879916E-5 24-25 0.0 0.0 0.0 0.002306838171645077 5.6264345649879916E-5 26-27 0.0 0.0 0.0 0.0032351998748680953 5.6264345649879916E-5 28-29 0.0 0.0 0.0 0.005457641528038352 5.6264345649879916E-5 30-31 0.0 0.0 0.0 0.011829578672887253 5.6264345649879916E-5 32-33 0.0 0.0 0.0 0.024249932975098247 5.6264345649879916E-5 34-35 0.0 0.0 0.0 0.04204353228687277 5.6264345649879916E-5 36-37 0.0 0.0 0.0 0.07016163902540026 5.6264345649879916E-5 38-39 0.0 0.0 0.0 0.11354144952145767 5.6264345649879916E-5 40-41 0.0 0.0 0.0 0.16139427549668056 5.6264345649879916E-5 42-43 0.0 0.0 0.0 0.21006293448382668 5.6264345649879916E-5 44-45 0.0 0.0 0.0 0.2695624800085747 5.6264345649879916E-5 46-47 0.0 0.0 0.0 0.3404977537866608 5.6264345649879916E-5 48-49 0.0 0.0 0.0 0.4199430098442912 5.6264345649879916E-5 50-51 0.0 0.0 0.0 0.5058867978244828 5.6264345649879916E-5 52-53 0.0 0.0 0.0 0.5898613337069286 5.6264345649879916E-5 54-55 0.0 0.0 0.0 0.685440390879662 5.6264345649879916E-5 56-57 0.0 0.0 0.0 0.7959716979088511 5.6264345649879916E-5 58-59 0.0 0.0 0.0 0.9055465110619924 5.6264345649879916E-5 60-61 0.0 0.0 0.0 1.0162606772145435 5.6264345649879916E-5 62-63 0.0 0.0 0.0 1.1263137373057086 5.6264345649879916E-5 64-65 0.0 0.0 0.0 1.241135200690701 5.6264345649879916E-5 66-67 0.0 0.0 0.0 1.3707682530680243 5.6264345649879916E-5 68-69 0.0 0.0 0.0 1.5155926787708154 5.6264345649879916E-5 70-71 0.0 0.0 0.0 1.6991832386263734 5.6264345649879916E-5 72-73 0.0 0.0 0.0 1.8988794674242098 5.6264345649879916E-5 74-75 0.0 0.0 0.0 2.1131622278317774 5.6264345649879916E-5 76-77 0.0 0.0 0.0 2.357644875766918 5.6264345649879916E-5 78-79 0.0 0.0 0.0 2.6478423045425865 5.6264345649879916E-5 80-81 0.0 0.0 0.0 2.9611503132939427 5.6264345649879916E-5 82-83 0.0 0.0 0.0 3.276680763698469 5.6264345649879916E-5 84-85 0.0 0.0 0.0 3.5996381077287802 5.6264345649879916E-5 86-87 0.0 0.0 0.0 3.933384140037455 5.6264345649879916E-5 88-89 0.0 0.0 0.0 4.29429178520861 5.6264345649879916E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 15435 0.0 35.704525 1 GTATCAA 23210 0.0 31.433764 1 GTACATG 17880 0.0 25.37979 1 ATCAACG 29470 0.0 24.601954 3 TCAACGC 29615 0.0 24.453243 4 TATCAAC 29740 0.0 24.447239 2 CAACGCA 30020 0.0 24.116737 5 TACATGG 18500 0.0 24.113937 2 AACGCAG 30380 0.0 23.784138 6 ACATGGG 18315 0.0 23.497774 3 CATGGGA 10420 0.0 22.134119 4 GAGTACT 18740 0.0 21.767593 12-13 ACGCAGA 34060 0.0 21.186548 7 GTACTTT 19335 0.0 21.085476 14-15 CGCAGAG 34500 0.0 20.916346 8 AGAGTAC 28240 0.0 20.41402 10-11 AGTACTT 19435 0.0 20.403774 12-13 GCAGAGT 35285 0.0 19.698542 9 GTATAGG 1180 0.0 19.308872 1 TAAGGTG 1145 0.0 19.0477 5 >>END_MODULE