FastQCFastQC Report
Fri 27 May 2016
SRR939425_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939425_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1387972
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59210.42659362004420837No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52790.3803390846501226No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34350.24748337862723455No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20340.14654474297752404No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT19720.1420777940765376No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73050.029.0875741
GTATCAA103050.027.8315871
GTACATG62850.026.7450081
TACATGG64750.024.8178632
GAGTACT62100.024.27998412-13
ACATGGG65900.023.8867383
TATCAAC123100.023.2995452
GTATAGG4500.023.2768861
ATCAACG124800.022.5428643
CATGGGA34250.022.4730244
TCAACGC126600.022.3177324
CAACGCA125100.022.2400935
AACGCAG125900.022.051476
GTACTTT70500.021.56588614-15
TACCTGG8550.021.0729732
ATGGGAG15850.020.382795
AGAGTAC97050.020.22652410-11
AGTACTT65450.020.11187212-13
ACGCAGA137350.019.728837
CGCAGAG138300.019.5182868
Produced by FastQC (version 0.11.5)