FastQCFastQC Report
Fri 27 May 2016
SRR939425_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939425_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1387972
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81400.5864671621617727No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81290.5856746389696622No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53520.3855985567432196No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30720.22133011328758795No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT29190.21030683616096005No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20620.14856207473925986No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18410.13263956333413066No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT18330.13206318283077756No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78250.032.5168881
CTATACG700.00850750227.0674
GTACATG67950.024.963911
GTATCAA107150.024.8963221
TACATGG69050.024.2196272
GAGTACT71400.023.45143312-13
ACATGGG70450.022.8599933
GTACTTT77800.021.46138214-15
TATCAAC130250.020.4779762
CGCATAG1301.242006E-820.41956166-67
ATCAACG129750.020.4071623
TCAACGC130850.020.235614
CAACGCA131850.020.0102845
AACGCAG132200.019.9573046
CATGGGA38900.019.7262084
AGTACTT74850.019.58605812-13
AGAGTAC111350.019.48289310-11
ACTTTTT89800.019.1473416-17
CATGGGG30850.018.4248644
ACGCAGA144050.018.1840257
Produced by FastQC (version 0.11.5)