FastQCFastQC Report
Fri 27 May 2016
SRR939424_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939424_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1380401
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58910.42676005015933777No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49480.35844656733804164No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33330.24145157820082713No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19340.1401042160937293No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT18920.13706162194898439No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67600.028.196741
GTATCAA93400.026.3154751
GTACATG63850.026.0818981
TACATGG64250.025.3232142
ACATGGG66800.023.1149023
TATCAAC112500.021.4546852
TCAACGC118000.021.1707124
GAGTACT60950.020.96190812-13
CAACGCA117750.020.91755
ATCAACG117550.020.8694523
AACGCAG119850.020.6531436
AGTACTT63650.020.07271612-13
CATGGGG28200.019.610794
GTACTTT68200.019.20114314-15
AGAGTAC95900.018.9661210-11
ACGCAGA130600.018.6564127
ATGGGAG17700.018.6269875
CGCAGAG131400.018.5059628
CATGGGA38750.018.3316174
ACCGGTC1350.00839783717.9407068
Produced by FastQC (version 0.11.5)