FastQCFastQC Report
Fri 27 May 2016
SRR939424_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939424_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1380401
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78060.5654878546161587No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77560.5618657187295576No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53670.3888000660677586No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29730.21537219981729946No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT27540.19950724463398678No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20550.14886978493930386No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT17880.12952757930485417No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17610.1275716259260896No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72600.030.721241
GTATCAA97150.024.6187061
GGACCGT1000.001671856723.7057616
TATACCG1401.8056826E-523.7057615
GTACATG68200.022.9618591
CGTAGAC1050.002213832522.5883733
TACATGG69600.022.4959032
ACATGGG70250.021.8777663
CGCATAG1601.4551915E-1121.01126366-67
CGCGGAA1950.020.66356782-83
GAGTACT67300.020.56560912-13
ACGCATA1552.237357E-1020.13798164-65
AGTACTT70650.019.92590712-13
AACGCAT2050.019.91128764-65
TCAACGC122250.019.5224174
ATCAACG122100.019.4248923
TATCAAC122900.019.3026452
GCACCGT3455.2750693E-1119.2394586
ATTCGCG1857.2759576E-1219.2184580-81
CATGGGG29450.019.1723844
Produced by FastQC (version 0.11.5)