##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939424_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1380401 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.85640549376594 31.0 31.0 31.0 28.0 34.0 2 31.108244633262363 31.0 31.0 34.0 28.0 34.0 3 31.104153792992037 31.0 31.0 34.0 28.0 34.0 4 35.02776584485233 35.0 35.0 37.0 32.0 37.0 5 34.59519733758524 35.0 35.0 37.0 30.0 37.0 6 34.65747416873793 35.0 35.0 37.0 31.0 37.0 7 34.49267133246064 35.0 35.0 37.0 30.0 37.0 8 34.40274094266811 35.0 35.0 37.0 30.0 37.0 9 35.896177270228 37.0 35.0 39.0 30.0 39.0 10-11 35.6883242622977 37.0 35.0 39.0 30.0 39.0 12-13 35.67375458290743 37.0 35.0 39.0 30.0 39.0 14-15 36.47594575779067 38.0 35.0 40.0 30.0 41.0 16-17 36.385148590880476 38.0 35.0 40.0 30.0 41.0 18-19 36.40161119848508 38.0 35.0 40.0 30.0 41.0 20-21 36.2903102069616 38.0 34.0 40.0 29.5 41.0 22-23 36.12915631037647 38.0 34.0 40.0 29.0 41.0 24-25 35.946229030549816 38.0 34.0 40.0 29.0 41.0 26-27 35.4699844465485 38.0 34.0 40.0 27.0 41.0 28-29 35.246973524359944 38.0 33.5 40.0 27.0 41.0 30-31 34.93694622069964 37.0 33.0 40.0 26.5 41.0 32-33 34.669875999800055 37.0 33.0 40.0 25.5 41.0 34-35 34.87465960978005 37.0 33.0 40.0 26.0 41.0 36-37 34.694971606076784 37.0 33.0 40.0 25.0 41.0 38-39 34.49531983822092 37.0 33.0 40.0 25.0 41.0 40-41 34.34043006343809 37.0 33.0 40.0 24.5 41.0 42-43 34.15248286548619 37.0 33.0 40.0 24.0 41.0 44-45 33.74037761491045 37.0 32.5 40.0 23.5 41.0 46-47 33.29928984403807 37.0 31.5 40.0 22.0 41.0 48-49 32.87657897958637 36.5 31.0 39.5 19.5 41.0 50-51 32.60905309399225 36.0 31.0 39.0 18.5 40.0 52-53 32.48914445874786 36.0 31.0 39.0 18.0 40.0 54-55 32.143142825889 36.0 30.5 39.0 16.5 40.0 56-57 31.644173685762325 35.0 29.5 39.0 13.5 40.0 58-59 31.181463574714883 35.0 29.0 38.5 10.5 40.0 60-61 30.802668572393095 35.0 28.0 38.0 8.0 40.0 62-63 30.623252953308494 34.0 28.0 38.0 8.0 40.0 64-65 30.178214518824603 34.0 28.0 38.0 7.0 40.0 66-67 29.295574981472775 33.5 27.0 37.0 4.0 39.5 68-69 25.83167101443711 31.0 16.5 35.5 2.0 39.0 70-71 24.499635975343395 29.5 12.0 35.0 2.0 38.0 72-73 25.523987594909016 30.0 18.0 35.0 2.0 37.5 74-75 26.28565503792014 31.0 21.5 35.0 2.0 37.0 76-77 25.961967935404274 30.5 22.0 34.0 2.0 36.0 78-79 27.09183164891941 32.0 25.0 35.0 2.0 37.0 80-81 27.094738775181995 32.0 25.0 35.0 2.0 36.0 82-83 26.769270306237104 32.0 25.0 35.0 2.0 36.0 84-85 26.407375103321428 32.0 24.5 34.5 2.0 35.5 86-87 25.964458153826314 31.5 23.5 34.0 2.0 35.0 88-89 25.47559549725044 31.0 21.0 34.0 2.0 35.0 90-91 24.863553778938147 31.0 19.5 34.0 2.0 35.0 92-93 24.28648740474688 31.0 17.5 34.0 2.0 35.0 94-95 23.437105232465058 30.0 7.0 34.0 2.0 35.0 96-97 22.766381652867537 30.0 2.0 34.0 2.0 35.0 98-99 21.901666254950555 29.5 2.0 34.0 2.0 35.0 100-101 20.498109969494372 27.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.30885870234198265 1101 2 0.24263812292306142 1101 3 0.3413904372848471 1101 4 0.4155406169860072 1101 5 0.4878647419419764 1101 6 0.39180179342834975 1101 7 0.47148888956333934 1101 8 0.7052243078644125 1101 9 0.5497788236500085 1101 10-11 0.7238486173697183 1101 12-13 0.6303667457839737 1101 14-15 0.6058291077596536 1101 16-17 0.6971389290722243 1101 18-19 0.4805635977202911 1101 20-21 0.6731185075983177 1101 22-23 0.6797214681132289 1101 24-25 0.7545047728672003 1101 26-27 0.6063307380100511 1101 28-29 0.6684554346603804 1101 30-31 0.366796593621288 1101 32-33 0.7055732312880778 1101 34-35 0.6149301840752486 1101 36-37 0.6982255189888349 1101 38-39 0.6922828089461674 1101 40-41 0.7201536954810521 1101 42-43 0.5194499863767703 1101 44-45 0.7538223511136053 1101 46-47 0.5680226133579538 1101 48-49 0.672729089024692 1101 50-51 0.384419461568001 1101 52-53 0.15247073866485295 1101 54-55 0.37168733460029557 1101 56-57 0.24778355784677686 1101 58-59 0.2966228360912986 1101 60-61 0.3692367404847303 1101 62-63 0.17770167076257692 1101 64-65 -0.15113040639494812 1101 66-67 -0.5564930913186217 1101 68-69 -7.51357100573615 1101 70-71 -7.911893756043696 1101 72-73 -4.959748205285326 1101 74-75 -3.0544121180731914 1101 76-77 -0.49236068698713353 1101 78-79 -0.2862920563711704 1101 80-81 -0.18891963451644145 1101 82-83 -0.6723047611506168 1101 84-85 -0.6968460968867802 1101 86-87 -0.6891096920955775 1101 88-89 -0.6948386116333332 1101 90-91 -0.9149519584139831 1101 92-93 -0.865177923846236 1101 94-95 -1.060675779906287 1101 96-97 -1.150631381499565 1101 98-99 -0.9711500627750382 1101 100-101 -1.0106577611856444 1102 1 0.28787683956892707 1102 2 0.2837740443917838 1102 3 0.30297478299484126 1102 4 0.272890566604822 1102 5 0.4554734417271433 1102 6 0.4436120306318472 1102 7 0.43854704022526647 1102 8 0.5972921228418784 1102 9 0.7159773255226654 1102 10-11 0.6818223114196655 1102 12-13 0.5941485350448588 1102 14-15 0.8072480986149984 1102 16-17 0.5692954814781643 1102 18-19 0.39544236601119565 1102 20-21 0.5239315775552953 1102 22-23 0.5747464052159614 1102 24-25 0.5354000845651399 1102 26-27 0.5827597100437387 1102 28-29 0.4687106676496029 1102 30-31 0.4964889356810218 1102 32-33 0.6169989871643011 1102 34-35 0.5367561693601388 1102 36-37 0.5462478197930309 1102 38-39 0.7341883109519074 1102 40-41 0.7709943325020276 1102 42-43 0.6367664594402527 1102 44-45 0.652911449765675 1102 46-47 0.7268382118801 1102 48-49 0.9320848594132229 1102 50-51 0.7358496218372039 1102 52-53 0.48413460109569684 1102 54-55 -0.04552235569799734 1102 56-57 0.3579615079979206 1102 58-59 0.49567723826624643 1102 60-61 0.567937444553877 1102 62-63 0.1731495404939345 1102 64-65 0.22431280545318444 1102 66-67 0.4068633096268961 1102 68-69 -6.642174527983087 1102 70-71 -7.47025354742123 1102 72-73 -1.3057409998251472 1102 74-75 0.2111906025108503 1102 76-77 0.5202258957742636 1102 78-79 0.38442292870459127 1102 80-81 0.2598824656502323 1102 82-83 0.0890725939321797 1102 84-85 -0.0902696885900447 1102 86-87 -0.22762080130551254 1102 88-89 -0.22264530314204833 1102 90-91 -0.1255975980649282 1102 92-93 -0.14986172645338414 1102 94-95 0.17416114665125448 1102 96-97 -0.12513065905237042 1102 98-99 -0.1498678140630858 1102 100-101 -0.05153187476194887 1103 1 0.37165369744394994 1103 2 0.19863375922193072 1103 3 0.26049498800173865 1103 4 0.40921452256213087 1103 5 0.4874353690172768 1103 6 0.4676163297692 1103 7 0.6342164572134337 1103 8 0.5306015199301086 1103 9 0.45360087878785293 1103 10-11 0.6011735922434482 1103 12-13 0.5162489640199155 1103 14-15 0.7014639753855718 1103 16-17 0.762468019564956 1103 18-19 0.728411962286529 1103 20-21 0.7506934729304291 1103 22-23 0.8308830013880453 1103 24-25 0.851499321421322 1103 26-27 0.8213583132934374 1103 28-29 0.7120542794881928 1103 30-31 0.7769407675077815 1103 32-33 0.9127758825863808 1103 34-35 0.878625590103006 1103 36-37 1.1029063199760714 1103 38-39 1.1729713640903725 1103 40-41 1.1659541102234883 1103 42-43 0.8623702629565386 1103 44-45 1.2729763592303485 1103 46-47 1.2644861707285386 1103 48-49 1.0925468758776162 1103 50-51 0.8467729976925682 1103 52-53 0.7638190650646095 1103 54-55 0.4787482928334441 1103 56-57 0.9051264709671472 1103 58-59 0.8392221961666131 1103 60-61 0.7582470199905984 1103 62-63 0.3567827808983175 1103 64-65 0.6208765462658903 1103 66-67 0.29640692522018597 1103 68-69 -6.640317122296587 1103 70-71 -7.957364348769165 1103 72-73 -0.9630162103233069 1103 74-75 0.5498849507409744 1103 76-77 0.6974714441020105 1103 78-79 0.7335823440970053 1103 80-81 0.6097505816330724 1103 82-83 0.3368655313318598 1103 84-85 0.18424384242851133 1103 86-87 0.20232813470492772 1103 88-89 0.4526231582736635 1103 90-91 0.2752570666868408 1103 92-93 0.1844830305702594 1103 94-95 0.2325374244243399 1103 96-97 0.2929545821404993 1103 98-99 0.059982654805715185 1103 100-101 0.1706704005893478 1104 1 0.3335242328132111 1104 2 0.2514171494154773 1104 3 0.17444837366855026 1104 4 0.22028404659247514 1104 5 0.3434686239608027 1104 6 0.2936268406278444 1104 7 0.3998171329114868 1104 8 0.4645360257983029 1104 9 0.5058046260414528 1104 10-11 0.45927148013498 1104 12-13 0.37831464584562724 1104 14-15 0.5951850622436865 1104 16-17 0.49849034409876225 1104 18-19 0.4392280995459785 1104 20-21 0.32195505198820484 1104 22-23 0.47828344081259644 1104 24-25 0.2936785580591774 1104 26-27 0.5000498107595703 1104 28-29 0.29432355399604404 1104 30-31 0.48204742180872984 1104 32-33 0.5860339175416058 1104 34-35 0.3624963352478048 1104 36-37 0.6957181954798131 1104 38-39 0.7727210135444054 1104 40-41 0.6760840606502114 1104 42-43 0.40819793787069614 1104 44-45 0.5223373718941673 1104 46-47 0.4632153725878112 1104 48-49 0.7929266800135011 1104 50-51 0.6300113799919345 1104 52-53 0.45024465547234627 1104 54-55 0.5397584724675539 1104 56-57 0.5171369487033317 1104 58-59 0.48538468388370504 1104 60-61 0.7423703238449448 1104 62-63 0.5481256755799571 1104 64-65 0.5444202623843815 1104 66-67 0.18321646007052905 1104 68-69 -6.593188010972021 1104 70-71 -3.998581102106254 1104 72-73 0.5107425257238596 1104 74-75 1.1782800022463924 1104 76-77 1.2403843430895698 1104 78-79 0.8636404187991751 1104 80-81 0.4012254140886604 1104 82-83 0.5407935783215798 1104 84-85 0.5302575551243507 1104 86-87 0.269293455495891 1104 88-89 0.1981612877382979 1104 90-91 -0.00556061628711646 1104 92-93 -0.03071079098803864 1104 94-95 0.17979267709345592 1104 96-97 0.20127301036966472 1104 98-99 0.29228422370593776 1104 100-101 0.3998267164892333 1105 1 0.028964013593068216 1105 2 0.023979073179468458 1105 3 0.1210713167159021 1105 4 0.11449791040393364 1105 5 0.24283845071254717 1105 6 0.06829122328100823 1105 7 0.008141741275608183 1105 8 0.39550199982940626 1105 9 0.3240421164110572 1105 10-11 0.3225622657208618 1105 12-13 0.16045898458703078 1105 14-15 0.22363824514067687 1105 16-17 0.16810374565147157 1105 18-19 0.25463503116949937 1105 20-21 0.2821945911969479 1105 22-23 0.20089997206585508 1105 24-25 0.30512778720461853 1105 26-27 0.5947112584365541 1105 28-29 0.6048341551864027 1105 30-31 0.5306706639978813 1105 32-33 0.4307355800362771 1105 34-35 0.22990878688419514 1105 36-37 0.21718585288907377 1105 38-39 0.31391368626351124 1105 40-41 0.2629960424082114 1105 42-43 0.015862176597138955 1105 44-45 0.1065274454661207 1105 46-47 0.010100205816257812 1105 48-49 0.13035439917014457 1105 50-51 0.008418703886306389 1105 52-53 -0.07016471726116436 1105 54-55 -0.44359259301345944 1105 56-57 -0.14777343575032376 1105 58-59 0.08922922045379167 1105 60-61 0.6002455139834311 1105 62-63 0.15929354210627267 1105 64-65 0.05236586372995333 1105 66-67 -0.0580442250770794 1105 68-69 -3.5766138619034535 1105 70-71 -3.702058202236916 1105 72-73 0.2977225199103515 1105 74-75 0.8340609175638605 1105 76-77 0.7349108947465588 1105 78-79 0.2530002835645071 1105 80-81 0.18839731056302256 1105 82-83 0.19606369490222164 1105 84-85 -0.11935708814905155 1105 86-87 -0.012252599642152973 1105 88-89 0.091508769161873 1105 90-91 0.27711709793086925 1105 92-93 0.36660031930780335 1105 94-95 0.4730518270227613 1105 96-97 0.33593338565151853 1105 98-99 0.42105630305189123 1105 100-101 0.43373697959291135 1106 1 0.08602834373710522 1106 2 0.18592860925022592 1106 3 0.08174003610587022 1106 4 0.08362150776707722 1106 5 0.31811691158337396 1106 6 0.20212437407351302 1106 7 0.259084271911469 1106 8 0.16968066941672788 1106 9 0.2586497913919459 1106 10-11 0.37922032197099753 1106 12-13 0.2681225997220835 1106 14-15 0.41313670559819826 1106 16-17 0.4576584149081526 1106 18-19 0.2730566384931734 1106 20-21 0.31021364432666587 1106 22-23 0.33531504924962974 1106 24-25 0.29483103780906816 1106 26-27 0.5406925359468389 1106 28-29 0.38780454224184524 1106 30-31 0.3950113471827734 1106 32-33 0.5655060952147224 1106 34-35 0.4557268108969268 1106 36-37 0.5217534377705562 1106 38-39 0.43510047719571787 1106 40-41 0.5876074370890407 1106 42-43 0.5669074122814663 1106 44-45 0.6777960697970897 1106 46-47 0.5467822334660255 1106 48-49 0.613059447629567 1106 50-51 0.4624897088252453 1106 52-53 0.33742202407503896 1106 54-55 0.49386147902473 1106 56-57 0.6600383474324971 1106 58-59 0.5960517701014503 1106 60-61 0.9109667224726081 1106 62-63 0.566600066046675 1106 64-65 0.6811534481981418 1106 66-67 0.6917024220267187 1106 68-69 -2.9264594943557682 1106 70-71 -3.4254470555360825 1106 72-73 0.7920456378455043 1106 74-75 1.1665584894263965 1106 76-77 1.3585014384427012 1106 78-79 0.9714592945376204 1106 80-81 0.9481894206661394 1106 82-83 0.946581353144456 1106 84-85 0.7046149314413661 1106 86-87 0.5964998543265594 1106 88-89 0.683255788235666 1106 90-91 0.949212115599444 1106 92-93 0.7384113998806363 1106 94-95 1.056286312774244 1106 96-97 1.0543027105259775 1106 98-99 1.0071117679110557 1106 100-101 1.0451360000719312 1107 1 0.023967905964578762 1107 2 0.06491639587408571 1107 3 0.07917501018227568 1107 4 -0.09351843626398448 1107 5 0.1893102647299969 1107 6 0.016549607819740686 1107 7 -0.10996620859702233 1107 8 0.037508076840204296 1107 9 0.3758548155800696 1107 10-11 0.030649265286179173 1107 12-13 -0.07534118317051508 1107 14-15 -0.12187294795842973 1107 16-17 -0.19758552721389577 1107 18-19 0.07211124074494535 1107 20-21 0.0599524559629927 1107 22-23 0.185182502663082 1107 24-25 0.27871480464856546 1107 26-27 0.6004001091077882 1107 28-29 0.28330229697643006 1107 30-31 0.3682250053384024 1107 32-33 0.28869719224923784 1107 34-35 0.24435687359081015 1107 36-37 0.2717130880731844 1107 38-39 0.4559705988956111 1107 40-41 0.3821148887670205 1107 42-43 0.11315315495452438 1107 44-45 0.4125744718622997 1107 46-47 0.521436549365113 1107 48-49 0.35477018802482974 1107 50-51 0.36019129461173804 1107 52-53 0.2276098887950866 1107 54-55 0.1639749354318738 1107 56-57 0.6004696119399497 1107 58-59 0.5940006604847738 1107 60-61 0.4913760766605151 1107 62-63 0.4405834055264677 1107 64-65 0.26514546503220515 1107 66-67 0.30479372948707706 1107 68-69 -2.4059709593101033 1107 70-71 -2.711546584789616 1107 72-73 0.981549727048705 1107 74-75 0.889509440681362 1107 76-77 0.47002952053047053 1107 78-79 0.5447254506866095 1107 80-81 0.21328910177336624 1107 82-83 0.4625780557498338 1107 84-85 0.3889629367345542 1107 86-87 0.42871496593902947 1107 88-89 0.3234512456407046 1107 90-91 0.18154936097333163 1107 92-93 0.22591015251095925 1107 94-95 0.4645536620594477 1107 96-97 0.5612474140858659 1107 98-99 0.6408130933769272 1107 100-101 0.8563369459035464 1108 1 -0.03657842131448419 1108 2 0.12542188337847548 1108 3 0.12149363847407457 1108 4 0.23069129140732514 1108 5 0.025800512645581364 1108 6 -0.016430325330084372 1108 7 0.1671352927638452 1108 8 0.17572394136831804 1108 9 0.18692457263786366 1108 10-11 0.1903870104042369 1108 12-13 0.10254643847529366 1108 14-15 0.1991986968045083 1108 16-17 0.39491452043251485 1108 18-19 0.2121694328861068 1108 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2312 90-91 2.1691927255060186 2312 92-93 2.4358354553480517 2312 94-95 2.3159255395208227 2312 96-97 2.4155613038628374 2312 98-99 2.493477594340341 2312 100-101 2.3171619710455467 2313 1 -0.33349627425950246 2313 2 -0.2013379442302501 2313 3 -0.07764875846119068 2313 4 -0.030877643336488347 2313 5 0.02660587592971808 2313 6 -0.1650742676921908 2313 7 -2.8842773579696868 2313 8 -3.517280281347279 2313 9 -1.1809087824620477 2313 10-11 -0.07190022132272844 2313 12-13 -0.004458045439214686 2313 14-15 -0.6841977595970477 2313 16-17 -0.5552617888269893 2313 18-19 0.04298872677840393 2313 20-21 -2.3892430619551632 2313 22-23 -0.7214251884413727 2313 24-25 0.07000930606034927 2313 26-27 -1.681582506497982 2313 28-29 -1.6518898550416807 2313 30-31 -1.1525098752280911 2313 32-33 -1.0350371985034457 2313 34-35 -1.4139506084579097 2313 36-37 -2.188042827085127 2313 38-39 -2.113965736726186 2313 40-41 -1.1947864508566326 2313 42-43 -0.07531669637727845 2313 44-45 0.034264660629006016 2313 46-47 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2314 7 -0.8312519149471953 2314 8 -0.22422601463565428 2314 9 -0.2971016684413783 2314 10-11 -0.29971329104562017 2314 12-13 -2.8288245061229205 2314 14-15 -0.7829390356569732 2314 16-17 -2.103263972982319 2314 18-19 -0.5893794406496369 2314 20-21 -0.967989768565225 2314 22-23 -0.8220872978222005 2314 24-25 -0.5266395671005029 2314 26-27 -2.287807945939882 2314 28-29 -1.2366713350754637 2314 30-31 -0.4894628293578478 2314 32-33 -0.2665971986227973 2314 34-35 -0.4727124430290388 2314 36-37 -1.1297755604719697 2314 38-39 -0.7625666241138802 2314 40-41 -0.843813278371023 2314 42-43 -0.816434613910495 2314 44-45 -4.278935604334098 2314 46-47 -4.519651237819193 2314 48-49 -4.380424876878621 2314 50-51 -4.732422134249759 2314 52-53 -1.5780136582198772 2314 54-55 -1.9504018012065742 2314 56-57 -1.624063925939005 2314 58-59 -2.1356705497579505 2314 60-61 -1.493191109604041 2314 62-63 -1.8339543871536215 2314 64-65 -2.220195267715461 2314 66-67 -1.6698520878661292 2314 68-69 -2.020837293550734 2314 70-71 1.0527427511423006 2314 72-73 0.25705725981953975 2314 74-75 -0.026936606667486984 2314 76-77 0.007649349322004895 2314 78-79 -2.3648579570072528 2314 80-81 -1.6934370887731731 2314 82-83 -0.7545751750033993 2314 84-85 -0.5976879201942147 2314 86-87 -0.6879159288811039 2314 88-89 -0.7019939424746902 2314 90-91 0.09079327099374268 2314 92-93 -0.563305817648704 2314 94-95 -0.13717420086031495 2314 96-97 0.2005162146928825 2314 98-99 0.046017601575098155 2314 100-101 -0.010441980539216189 2315 1 -0.3842291288879345 2315 2 -0.27716698116113747 2315 3 -0.45397467238776557 2315 4 -0.25992391987423247 2315 5 -0.28768362111861023 2315 6 -0.1137027937276116 2315 7 -1.0556952486567468 2315 8 -2.6874723344305664 2315 9 -0.7940285157995319 2315 10-11 -1.5761688464220214 2315 12-13 -0.676002881189433 2315 14-15 -0.3508112079857 2315 16-17 -0.11872808997495099 2315 18-19 -0.15810938695813803 2315 20-21 -0.5999313689908874 2315 22-23 -0.22134964444673244 2315 24-25 -4.728495390128188 2315 26-27 -0.7803403140087681 2315 28-29 -0.7420356471632985 2315 30-31 -1.0550744770255704 2315 32-33 -0.2298386813463864 2315 34-35 -2.1580021029829197 2315 36-37 -3.6981548191101723 2315 38-39 -1.1317980134819976 2315 40-41 -0.169193391558494 2315 42-43 -1.482713777419633 2315 44-45 -5.138788718601845 2315 46-47 -3.3918001873685384 2315 48-49 -2.524206015525209 2315 50-51 -1.0110527593606093 2315 52-53 -0.1665199705979461 2315 54-55 -1.314664712156091 2315 56-57 -1.054279498083929 2315 58-59 -0.542939055586622 2315 60-61 -1.651504422836652 2315 62-63 -0.6139651899462244 2315 64-65 0.11924459942631671 2315 66-67 0.5007997881113617 2315 68-69 3.2691072413648605 2315 70-71 3.4235437701519906 2315 72-73 -2.6975992274992713 2315 74-75 -2.205359237711807 2315 76-77 -0.5354997453394823 2315 78-79 -0.38127820176306315 2315 80-81 -0.7054951013818034 2315 82-83 -1.268955788021568 2315 84-85 -0.7773827009942629 2315 86-87 -0.2915912969016148 2315 88-89 -0.19252698773355448 2315 90-91 -0.11608826912976511 2315 92-93 -1.404068623795009 2315 94-95 -0.22258317523995785 2315 96-97 -0.49834304265543494 2315 98-99 -2.5840069062528954 2315 100-101 -3.8218631120914317 2316 1 -0.4457989555953752 2316 2 -0.486850977083666 2316 3 -0.805146945578386 2316 4 -0.4755202501494651 2316 5 -0.23549197993614257 2316 6 -0.4124795521373983 2316 7 -0.7150224657821269 2316 8 -0.9637210602205784 2316 9 -4.619308841997292 2316 10-11 -1.464751924913358 2316 12-13 -0.647103798620627 2316 14-15 -0.36814046384706245 2316 16-17 -0.43131728467423613 2316 18-19 -0.35897584975120367 2316 20-21 -3.510583764505668 2316 22-23 -1.07440774842226 2316 24-25 -0.4843057876817056 2316 26-27 -0.3385971947427109 2316 28-29 -0.15849844023160387 2316 30-31 -0.02138436489509843 2316 32-33 -1.343039496840781 2316 34-35 -0.7300204516067765 2316 36-37 -0.4935676631468695 2316 38-39 -5.497139501759268 2316 40-41 -5.224850883913234 2316 42-43 -3.856006336033289 2316 44-45 -2.4122851508138723 2316 46-47 -2.8448582913440745 2316 48-49 -2.8486708473294904 2316 50-51 -1.1950996502882347 2316 52-53 -0.6392518768160862 2316 54-55 -0.044073478720825676 2316 56-57 -3.614014462406047 2316 58-59 -0.803183703904665 2316 60-61 -0.7478346980660113 2316 62-63 -0.24260943051706718 2316 64-65 -0.11893073186828218 2316 66-67 -0.14981183268374565 2316 68-69 2.320177577756297 2316 70-71 3.6738495805495432 2316 72-73 2.5603008744375266 2316 74-75 1.239798764326924 2316 76-77 -1.6793325689888228 2316 78-79 0.064848199867928 2316 80-81 0.08614608108506516 2316 82-83 0.3726344260457104 2316 84-85 0.8771534865694477 2316 86-87 0.9212527397956336 2316 88-89 0.7076768858648137 2316 90-91 0.6576120366806322 2316 92-93 0.7798763303334333 2316 94-95 0.9001487309781844 2316 96-97 1.1287721459276447 2316 98-99 1.0811307084260022 2316 100-101 1.1744671631379227 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 19.0 4 66.0 5 137.0 6 307.0 7 798.0 8 1670.0 9 2772.0 10 4379.0 11 6270.0 12 8147.0 13 10024.0 14 12381.0 15 15570.0 16 18146.0 17 19943.0 18 18705.0 19 16574.0 20 15993.0 21 16539.0 22 17454.0 23 19204.0 24 22908.0 25 28167.0 26 35437.0 27 43398.0 28 52894.0 29 62569.0 30 73214.0 31 84266.0 32 96756.0 33 110762.0 34 127537.0 35 140425.0 36 140883.0 37 110201.0 38 43376.0 39 2510.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.598381591179326 20.458278658175626 15.363507982271118 22.579831768373932 2 16.292000504288627 21.823162788136464 38.1106710661502 23.774165641424702 3 19.453823791271642 25.06684671148952 31.0321595888719 24.44716990836694 4 13.18990693452355 17.720142338846497 36.3404154739843 32.74953525264565 5 13.08474856219316 38.30444921439495 34.20353940630295 14.407262817108943 6 31.40978599696755 36.60943450490111 16.838730195066507 15.142049303064834 7 27.15435587195315 33.87037534745338 20.784467701776514 18.190801078816953 8 26.340534380951624 35.04793172418739 19.513242891015004 19.098291003845986 9 26.07003327294025 16.502233771201265 20.704273613247164 36.723459342611314 10-11 25.185329281881636 26.84586660638756 26.8377521108556 21.131052000875204 12-13 25.832312494702624 24.63892738414417 27.742518297219434 21.786241823933768 14-15 22.705141477005593 25.870018929282146 26.6360644479394 24.78877514577286 16-17 21.537321396166096 29.064734755173248 27.371052260784868 22.026891587875784 18-19 21.217995666470348 27.069564669345603 30.47416000736019 21.23827965682386 20-21 22.24784682132221 25.99632280764792 30.51182228932028 21.24400808170959 22-23 21.825098142056234 26.143203730521 30.396219348491776 21.635478778930988 24-25 21.84596771856617 26.353885942797532 30.546668984902492 21.253477353733807 26-27 21.616958665028914 26.44535476469823 30.62221061481391 21.315475955458947 28-29 21.259428106960364 26.826407646297522 30.672557313001743 21.24160693374037 30-31 21.406326291402912 26.32552350843742 30.568068049939217 21.700082150220442 32-33 21.445574880178963 26.585129441491983 30.37948640675982 21.589809271569234 34-35 21.942286690223366 26.59253128265685 30.171169425311923 21.294012601807864 36-37 21.924376623946326 26.30126884883482 30.31326597607898 21.461088551139877 38-39 21.57078727541496 26.757676037724014 30.379063904647918 21.292472782213107 40-41 21.67851408730756 26.618179788127588 30.323173757779003 21.380132366785848 42-43 21.505965742658105 26.770110092168835 29.82396418814276 21.899959977030296 44-45 21.72436157011536 26.83386702735271 29.643817698871867 21.797953703660067 46-47 21.9178901576944 27.194235997824904 29.196084828711257 21.691789015769437 48-49 22.061921293569164 27.0934024884082 29.040020212924457 21.80465600509818 50-51 21.672531867105725 27.454938005773847 29.01951103513175 21.853019091988674 52-53 22.12939478573256 27.552460529110345 28.61432006268661 21.70382462247048 54-55 21.63533408833522 27.24642355605889 28.84403171007928 22.274210645526612 56-57 22.009605783180024 27.544054283979296 28.562277445974715 21.88406248686596 58-59 22.269787885561747 27.14218482182742 28.4862730833455 22.10175420926533 60-61 22.00569262642945 27.256790922227943 29.106514133935757 21.631002317406853 62-63 21.81614811079912 27.317310116403405 29.003123795614517 21.863417977182962 64-65 21.651270333338886 27.390500808878027 28.748061119736406 22.21016773804668 66-67 21.49978358284496 27.53348660916586 28.67572655755953 22.291003250429657 68-69 21.720449256475934 26.913458243576066 29.3119524016143 22.0541400983337 70-71 21.29500342131273 27.028809207717547 29.634343546585413 22.041843824384312 72-73 21.741362261516983 27.176411993950673 28.891126686831086 22.191099057701255 74-75 21.588986206664103 27.710728097792465 28.328110259706417 22.372175435837015 76-77 21.03777663160516 27.570476609790774 28.84710803346421 22.54463872513986 78-79 21.704740009868228 27.629770458640753 28.304352960866897 22.361136570624126 80-81 21.60976014390569 27.733612200107437 28.179224304586846 22.477403351400028 82-83 21.647830649347263 27.72967704147812 28.155176279549416 22.46731602962521 84-85 21.531484182658716 27.639100130071043 28.0878108112905 22.741604875979743 86-87 21.61952368015938 27.65462283799692 28.14778592773703 22.578067554106674 88-89 21.47057289440551 27.58969540580443 27.87367229935801 23.066059400432053 90-91 21.432568895238376 27.661367121180785 27.992952453329384 22.913111530251456 92-93 21.64657547842674 27.622205245623967 28.024101456787736 22.707117819161557 94-95 21.369396214992488 27.32386809569642 28.134594156638627 23.172141532672473 96-97 21.28299712650967 27.291055944283997 28.201042863199394 23.224904066006935 98-99 21.339557091570395 27.243324312868832 27.822445379094983 23.59467321646579 100-101 21.08049538856239 27.034301676665752 28.1918071133404 23.693395821431455 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2618.0 1 1792.0 2 1022.5 3 1118.0 4 1060.0 5 979.5 6 1374.5 7 2396.0 8 3380.0 9 3868.5 10 3556.5 11 2907.0 12 2655.5 13 2559.5 14 2582.5 15 2690.5 16 2797.5 17 2980.5 18 3248.0 19 3434.5 20 3701.5 21 4282.5 22 5009.0 23 5820.0 24 6698.0 25 7899.5 26 9700.5 27 11635.0 28 13387.5 29 15306.0 30 17377.0 31 19757.0 32 22386.5 33 24643.0 34 26787.5 35 29056.0 36 31818.0 37 34681.5 38 36093.5 39 36183.5 40 36823.5 41 38817.0 42 40933.5 43 42691.5 44 44470.0 45 45717.0 46 46666.5 47 49539.0 48 54656.5 49 55187.0 50 51434.0 51 48747.0 52 47119.5 53 46690.0 54 45370.0 55 42041.5 56 38049.0 57 34295.5 58 30654.5 59 26867.0 60 22905.0 61 18428.0 62 14618.0 63 11647.5 64 8902.0 65 6757.5 66 5037.5 67 3883.0 68 2905.5 69 2039.0 70 1509.5 71 1213.5 72 1012.0 73 817.5 74 664.0 75 525.0 76 349.5 77 196.5 78 101.5 79 59.0 80 42.5 81 32.5 82 19.5 83 11.0 84 7.5 85 6.0 86 3.5 87 1.5 88 0.5 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.020283960964965975 2 0.0173138095379531 3 2.897708709280854E-4 4 0.0838162244159487 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.01122862124846331 12-13 0.0 14-15 0.0 16-17 4.7087766525813875E-4 18-19 1.448854354640427E-4 20-21 0.0 22-23 2.897708709280854E-4 24-25 0.0029701514270128754 26-27 0.004093013551859205 28-29 0.0014850757135064377 30-31 2.1732815319606405E-4 32-33 6.519844595881921E-4 34-35 0.003151258221342929 36-37 0.08964786319337642 38-39 0.10036938541771558 40-41 0.08167916424285407 42-43 0.08642416225430147 44-45 0.08530130012945514 46-47 0.08338156810955658 48-49 0.07863657009810918 50-51 0.07816569243285104 52-53 0.06085188289489793 54-55 0.05168787910179723 56-57 0.02890464437507652 58-59 0.023217891033112843 60-61 0.014090108598878152 62-63 0.0021008388142286193 64-65 0.006809615466810007 66-67 0.16484340419921456 68-69 0.7440229324667252 70-71 0.9058961852389269 72-73 0.3651837400871196 74-75 0.05690375477850277 76-77 0.20787437853203528 78-79 0.015502741594652569 80-81 0.0038032426809311206 82-83 0.0016299611489704803 84-85 1.0866407659803202E-4 86-87 0.001883510661032555 88-89 5.070990241241494E-4 90-91 0.004527669858251334 92-93 0.005034768882375484 94-95 0.08283824772656641 96-97 0.039553723881683656 98-99 0.014923199852796398 100-101 0.013872780445682088 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1380401.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.31450530151254 #Duplication Level Percentage of deduplicated Percentage of total 1 80.61318944549713 47.81531452736774 2 11.783085604888234 13.978157871586387 3 3.294977375914051 5.863198590960535 4 1.3906235559103692 3.299365931178153 5 0.7024508770298752 2.0832763134820333 6 0.45424165329129895 1.6165871371388745 7 0.29284149601456266 1.215882392750105 8 0.2082672225861281 0.9882613822572949 9 0.16315793814650623 0.870986914845732 >10 0.9640838868285727 10.948372182890912 >50 0.08801401404704653 3.5654203327388947 >100 0.04197169751914421 4.490246632300608 >500 0.0018571393962955544 0.7319452804143242 >1k 8.66665051604592E-4 0.9960242638968074 >5k 3.7142787925911085E-4 1.5369602461915712 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7806 0.5654878546161587 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7756 0.5618657187295576 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5367 0.3888000660677586 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2973 0.21537219981729946 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2754 0.19950724463398678 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2055 0.14886978493930386 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1788 0.12952757930485417 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1761 0.1275716259260896 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 3.622135886601068E-5 0.0 22-23 0.0 0.0 0.0 7.244271773202135E-5 0.0 24-25 0.0 0.0 0.0 3.2599222979409607E-4 0.0 26-27 0.0 0.0 0.0 9.055339716502668E-4 0.0 28-29 0.0 0.0 0.0 0.0021008388142286193 0.0 30-31 0.0 0.0 0.0 0.007026943620006071 0.0 32-33 0.0 0.0 0.0 0.016806710513828954 0.0 34-35 0.0 0.0 0.0 0.03230945210848152 0.0 36-37 0.0 0.0 0.0 0.05875104408066932 0.0 38-39 0.0 0.0 0.0 0.09518973109987605 0.0 40-41 0.0 0.0 0.0 0.132352845296403 0.0 42-43 0.0 0.0 0.0 0.16886397503334177 0.0 44-45 0.0 0.0 0.0 0.21845101532091038 0.0 46-47 0.0 0.0 0.0 0.28111396615910883 0.0 48-49 0.0 0.0 0.0 0.35055031110525126 0.0 50-51 0.0 0.0 0.0 0.4228119220429426 0.0 52-53 0.0 0.0 0.0 0.49601528831115016 0.0 54-55 0.0 0.0 0.0 0.5754125069454455 0.0 56-57 0.0 0.0 0.0 0.6720510923999621 0.0 58-59 0.0 0.0 0.0 0.7660817400161257 0.0 60-61 0.0 0.0 0.0 0.8634447526479625 0.0 62-63 0.0 0.0 0.0 0.9579462779293842 0.0 64-65 7.244271773202135E-5 0.0 0.0 1.0599818458549364 0.0 66-67 7.244271773202135E-5 0.0 0.0 1.1679577166345143 0.0 68-69 7.244271773202135E-5 0.0 0.0 1.2970868609918422 0.0 70-71 7.244271773202135E-5 0.0 0.0 1.4625822496506449 0.0 72-73 7.244271773202135E-5 0.0 0.0 1.642095304190594 0.0 74-75 7.244271773202135E-5 0.0 0.0 1.8280195392498269 0.0 76-77 7.244271773202135E-5 0.0 0.0 2.0381758633904203 0.0 78-79 7.244271773202135E-5 0.0 0.0 2.2899143075091946 0.0 80-81 7.244271773202135E-5 0.0 0.0 2.569651862031395 0.0 82-83 7.244271773202135E-5 0.0 0.0 2.862030670797833 0.0 84-85 7.244271773202135E-5 0.0 0.0 3.149266046605298 0.0 86-87 7.244271773202135E-5 0.0 0.0 3.4503379814995787 0.0 88-89 7.244271773202135E-5 0.0 0.0 3.785566657804508 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7260 0.0 30.72124 1 GTATCAA 9715 0.0 24.618706 1 GGACCGT 100 0.0016718567 23.705761 6 TATACCG 140 1.8056826E-5 23.705761 5 GTACATG 6820 0.0 22.961859 1 CGTAGAC 105 0.0022138325 22.588373 3 TACATGG 6960 0.0 22.495903 2 ACATGGG 7025 0.0 21.877766 3 CGCATAG 160 1.4551915E-11 21.011263 66-67 CGCGGAA 195 0.0 20.663567 82-83 GAGTACT 6730 0.0 20.565609 12-13 ACGCATA 155 2.237357E-10 20.137981 64-65 AGTACTT 7065 0.0 19.925907 12-13 AACGCAT 205 0.0 19.911287 64-65 TCAACGC 12225 0.0 19.522417 4 ATCAACG 12210 0.0 19.424892 3 TATCAAC 12290 0.0 19.302645 2 GCACCGT 345 5.2750693E-11 19.239458 6 ATTCGCG 185 7.2759576E-12 19.21845 80-81 CATGGGG 2945 0.0 19.172384 4 >>END_MODULE