FastQCFastQC Report
Fri 27 May 2016
SRR939422_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939422_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126215
Sequences flagged as poor quality0
Sequence length101
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA59764.73477795824585No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38263.0313354197203184No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34182.7080774868280315No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26962.136037713425504No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25211.997385413778077No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT14671.1623024204730024No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT11240.8905439131640455No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11060.8762825337717387No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT9100.7209919581666204No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT7290.5775858653884245No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6900.5466862100384264No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6390.5062789684268906No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6320.5007328764409935No Hit
ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT5160.4088262092461276RNA PCR Primer, Index 31 (95% over 22bp)
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC4990.39535712870894907No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT4610.3652497722140791No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC4540.3597036802281821No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC4440.3517806916769005No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC4100.3248425306025433No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4070.32246563403715883No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT3830.3034504615140831No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA3710.29394287525254525No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG3420.2709662084538288No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA3340.2646278176128036No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA3310.2622509210474191No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA3260.25828942677177835No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC3260.25828942677177835No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3110.246404943944856No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT3080.24402804737947154No Hit
ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCT2730.21629758744998615RNA PCR Primer, Index 31 (95% over 24bp)
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC2590.20520540347819197No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG2460.19490551836152595No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA2460.19490551836152595No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT2430.1925286217961415No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA2410.1909440240858852No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC2280.1806441389692192No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1920.15212138018460564No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC1900.15053678247434932No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG1790.14182149506793962No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT1760.13944459850255517No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1650.13072931109614547No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT1590.12597551796537654No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1520.12042942597947946No Hit
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG1510.1196371271243513No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1460.11567563284871052No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG1450.11488333399358237No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA1420.11250643742819792No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA1410.11171413857306978No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT1320.10458344887691637No Hit
GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1280.10141425345640376No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTATGA200.002082953271.136199
TAGTGTA250.005042757456.9089556
AGTGTAT250.005042757456.9089557
GGGGCAG451.5225596E-552.6934787
CCTTAAG405.380678E-447.4805641
TGGGGCA502.8392975E-547.424136
CTGTGCG405.4123433E-447.424139
ATGGGGC652.5994377E-643.776125
TCTGAAC459.611782E-442.204953
CAACTCA608.326905E-539.5201076
TGGGCAG651.3339818E-436.48018
GTACATA550.002576122834.531321
GCCTGTG550.002591202734.4902767
ATGGGCA851.6385951E-533.4758537
GGGCAGT753.0919112E-431.6160878
TTGTGGT600.003968688731.6160859
AAGCCTC404.5259917E-429.6400824-25
GGCAGTG856.4352545E-427.8965449
AGACTCG350.0080760227.34871764-65
TTAAGCC700.00838046627.131753
Produced by FastQC (version 0.11.5)