FastQCFastQC Report
Fri 27 May 2016
SRR939421_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939421_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3067474
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113790.3709566894454525No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87970.28678319685839226No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61770.20137089996524826No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32670.10650457020988605No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA84100.045.4462281
GTATCAA138950.039.475251
TATCAAC175000.030.9627672
ATCAACG176900.030.575893
TCAACGC182250.029.6581274
CAACGCA183550.029.367385
AACGCAG187950.028.820346
ACGCAGA211050.025.2885277
CGCAGAG213200.024.6701748
GTACATG130650.024.6147231
TACATGG129100.023.9349382
GCAGAGT214850.022.935319
GAGTACT120200.022.62337512-13
ACATGGG134650.021.9884763
GTACTTT130950.021.0727614-15
AGTACTT124950.020.3827612-13
CAGAGTA208750.019.9845810-11
AGAGTAC196300.019.58940510-11
ACTTTTT157100.018.74779716-17
CATGGGA88150.018.628984
Produced by FastQC (version 0.11.5)