FastQCFastQC Report
Fri 27 May 2016
SRR939421_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939421_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3067474
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160330.5226776168273961No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138640.4519679710406673No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100330.32707693691943274No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA49130.160164356731304No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37560.12244602562238506No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31200.10171235355214095No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA87550.045.583541
GTATCAA143700.036.54561
ATCAACG183150.028.3248863
TATCAAC185200.028.0419642
TCAACGC185600.027.8744094
CAACGCA190300.027.185975
AACGCAG194450.026.6301256
ACGCAGA221250.023.2759427
CGCAGAG224000.022.8209888
GTACATG143050.021.7685591
GAGTACT131050.021.47457312-13
TACATGG141250.021.2015442
GCAGAGT234450.021.0561269
GTGGTAT49700.020.9806251
TGGTATC49450.020.6020772
ACATGGG143200.020.214323
GTACTTT140550.020.07363514-15
AGTACTT136000.020.04848512-13
AGAGTAC218050.018.8375310-11
CAGAGTA231550.018.77250710-11
Produced by FastQC (version 0.11.5)