##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939421_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3067474 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.993296112697287 31.0 31.0 31.0 30.0 34.0 2 31.254000196904684 31.0 31.0 34.0 30.0 34.0 3 31.241104244078354 31.0 31.0 34.0 28.0 34.0 4 35.176872892810174 35.0 35.0 37.0 33.0 37.0 5 34.795185224063836 35.0 35.0 37.0 32.0 37.0 6 34.75170188891577 35.0 35.0 37.0 32.0 37.0 7 34.624814423854936 35.0 35.0 37.0 30.0 37.0 8 34.64217887421377 35.0 35.0 37.0 30.0 37.0 9 36.00220246365576 37.0 35.0 39.0 30.0 39.0 10-11 35.862663709619056 37.0 35.0 39.0 30.0 39.0 12-13 35.82389337285336 37.0 35.0 39.0 30.0 39.0 14-15 36.68661022065713 38.0 36.0 40.0 31.0 41.0 16-17 36.69404386149647 38.0 36.0 40.0 31.0 41.0 18-19 36.652205854067546 38.0 35.0 40.0 30.0 41.0 20-21 36.552992625202364 38.0 35.0 40.0 30.0 41.0 22-23 36.41203658123916 38.0 35.0 40.0 30.0 41.0 24-25 36.19444174587951 38.0 34.0 40.0 29.5 41.0 26-27 35.81779633666007 38.0 34.0 40.0 27.5 41.0 28-29 35.5390976419034 38.0 34.0 40.0 27.0 41.0 30-31 35.32247119290987 38.0 34.0 40.0 27.0 41.0 32-33 35.11787614173747 37.5 33.0 40.0 27.0 41.0 34-35 35.078121607550706 38.0 33.0 40.0 26.5 41.0 36-37 35.08113646603036 38.0 33.5 40.0 26.5 41.0 38-39 35.058207991330974 38.0 33.0 40.0 26.0 41.0 40-41 34.84384676121134 38.0 33.0 40.0 25.5 41.0 42-43 34.54235814223691 38.0 33.0 40.0 24.5 41.0 44-45 34.37011658452525 37.5 33.0 40.0 24.0 41.0 46-47 34.05732338725609 37.0 33.0 40.0 24.0 41.0 48-49 33.751556981412065 37.0 32.5 40.0 23.0 41.0 50-51 33.49861416918285 37.0 32.0 40.0 22.5 41.0 52-53 33.12784982040597 37.0 31.0 40.0 20.5 41.0 54-55 32.92188409746912 36.5 31.0 39.0 20.5 41.0 56-57 32.53403940832098 36.0 31.0 39.0 19.0 40.0 58-59 32.1057810106948 35.5 30.5 39.0 17.5 40.0 60-61 31.825346359903946 35.0 30.0 39.0 16.0 40.0 62-63 31.42937984152433 35.0 29.0 38.5 13.0 40.0 64-65 30.998686052432717 35.0 29.0 38.0 10.0 40.0 66-67 29.308891778707824 33.5 26.0 37.5 7.0 39.5 68-69 25.636266680662985 30.0 16.5 35.0 4.0 39.0 70-71 25.029753634423635 29.5 15.0 35.0 2.0 38.0 72-73 26.86853287102026 31.0 22.0 35.0 2.0 38.0 74-75 27.481380934280125 32.0 24.5 35.0 2.0 38.0 76-77 27.531420967219283 31.5 25.5 34.5 2.0 36.5 78-79 28.365023468821576 33.0 26.5 35.0 2.0 37.0 80-81 28.2213107592762 33.0 26.5 35.0 2.0 37.0 82-83 27.81217020910365 33.0 26.0 35.0 2.0 36.0 84-85 27.47713933353632 32.5 26.0 35.0 2.0 36.0 86-87 27.022733363021167 32.0 25.5 35.0 2.0 35.5 88-89 26.53330574277076 32.0 25.0 34.0 2.0 35.0 90-91 26.073809101560435 32.0 24.0 34.0 2.0 35.0 92-93 25.51504609330022 31.5 21.5 34.0 2.0 35.0 94-95 24.143080430347574 31.0 15.0 34.0 2.0 35.0 96-97 23.335279777432508 30.0 4.5 34.0 2.0 35.0 98-99 23.004930604138778 30.5 2.0 34.0 2.0 35.0 100-101 21.738481238960787 30.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.43251002779933145 1101 2 0.31584965067871096 1101 3 0.3751276064692597 1101 4 0.3427651418932314 1101 5 0.43096406948676247 1101 6 0.6547074703957847 1101 7 0.5537655000535509 1101 8 0.6235791372936887 1101 9 0.7540693542893706 1101 10-11 0.8736449638553694 1101 12-13 0.7420769235768887 1101 14-15 0.7298208834199258 1101 16-17 0.7919895424797474 1101 18-19 0.9150952174079592 1101 20-21 0.8332760714496672 1101 22-23 0.8122616359391372 1101 24-25 0.7755465017002834 1101 26-27 0.8595117252311866 1101 28-29 0.7916194048037681 1101 30-31 0.8170524082404427 1101 32-33 0.7815932371689058 1101 34-35 0.9558203741953264 1101 36-37 0.8443318751095248 1101 38-39 0.7586140027741592 1101 40-41 0.9872474976815511 1101 42-43 0.9014604925600693 1101 44-45 0.6627097975634371 1101 46-47 0.6145199848134411 1101 48-49 0.5727843642776307 1101 50-51 0.5067113981788935 1101 52-53 0.6528609571916135 1101 54-55 0.5641748521081809 1101 56-57 0.471842416428764 1101 58-59 0.32505192830726415 1101 60-61 0.1863806228592857 1101 62-63 0.03792851474286252 1101 64-65 -0.20537379730558314 1101 66-67 -3.0833610140489185 1101 68-69 -7.896717351349437 1101 70-71 -11.421433158979427 1101 72-73 -11.385813984872696 1101 74-75 -9.67609168193848 1101 76-77 -4.56854860139773 1101 78-79 -1.5844683705144256 1101 80-81 -1.0037931377188478 1101 82-83 -0.6787432141172687 1101 84-85 -0.71607270092154 1101 86-87 -0.6282056968651837 1101 88-89 -0.6865249307435946 1101 90-91 -0.6551969107956666 1101 92-93 -1.176369243258307 1101 94-95 -4.994391340888651 1101 96-97 -1.8788712690836569 1101 98-99 -0.9957648810293271 1101 100-101 -0.616535075737854 1102 1 0.3482062205023375 1102 2 0.277504471518899 1102 3 0.35177683373683877 1102 4 0.20566759868192008 1102 5 0.4555130815453694 1102 6 0.38309770049173153 1102 7 0.4157845963834106 1102 8 0.4802740076843577 1102 9 0.7022955754334461 1102 10-11 0.6568780130403411 1102 12-13 0.6678261161183414 1102 14-15 0.6665056758977528 1102 16-17 0.6718178565664061 1102 18-19 0.67899081287878 1102 20-21 0.7764954502401764 1102 22-23 0.8007669471047549 1102 24-25 0.7203479231636791 1102 26-27 0.7408540520536206 1102 28-29 0.7024491535572679 1102 30-31 0.4897212445894894 1102 32-33 0.397298339512588 1102 34-35 0.5841062738301943 1102 36-37 0.7936970274294879 1102 38-39 0.797109778119534 1102 40-41 0.6925832721610092 1102 42-43 0.7799217672004559 1102 44-45 0.4619057293284712 1102 46-47 0.626729949620362 1102 48-49 0.6463038387304962 1102 50-51 0.515815020341428 1102 52-53 0.6081999520231207 1102 54-55 0.5531995344964855 1102 56-57 0.5489707873546408 1102 58-59 0.5991600652244706 1102 60-61 0.4308825873634987 1102 62-63 0.3591437378479547 1102 64-65 0.4888209008857025 1102 66-67 -2.7998153223521847 1102 68-69 -7.676492279155411 1102 70-71 -11.408890782277314 1102 72-73 -9.54375185748308 1102 74-75 -8.003178814467237 1102 76-77 -4.267385660708204 1102 78-79 -1.4506512478667801 1102 80-81 -0.8910830522381836 1102 82-83 -0.6808810806089873 1102 84-85 -0.5886418047566018 1102 86-87 -0.5052875873764329 1102 88-89 -0.362293266488912 1102 90-91 -0.5272419023341932 1102 92-93 -1.031821907571853 1102 94-95 -4.5118712776480905 1102 96-97 -1.5015232522569555 1102 98-99 -0.7982078222054838 1102 100-101 -0.63403576587719 1103 1 0.2923361191181719 1103 2 0.2571052158201006 1103 3 0.33836146146504475 1103 4 0.24364450665631665 1103 5 0.3623445778373835 1103 6 0.39060622704021597 1103 7 0.2997468691507592 1103 8 0.3966383063785983 1103 9 0.6258683160517862 1103 10-11 0.6415094121671245 1103 12-13 0.48754832399644954 1103 14-15 0.6736736323047765 1103 16-17 0.6814491063973449 1103 18-19 0.6016723012844665 1103 20-21 0.6129953042815472 1103 22-23 0.6799607109651262 1103 24-25 0.764031225250271 1103 26-27 0.7130349251848287 1103 28-29 0.8752046312213935 1103 30-31 0.8305192378427222 1103 32-33 0.7252881554290695 1103 34-35 0.8822716563770499 1103 36-37 0.7580961187272308 1103 38-39 0.9091429959154524 1103 40-41 0.8947614895828124 1103 42-43 0.8723054578426357 1103 44-45 0.6474154182767506 1103 46-47 0.678136325149616 1103 48-49 0.8049641650665933 1103 50-51 0.7583234432610482 1103 52-53 0.8505641727322413 1103 54-55 0.7641684222987379 1103 56-57 0.7168041131353604 1103 58-59 0.6683078354297258 1103 60-61 0.5832954978348255 1103 62-63 0.6389009880927112 1103 64-65 0.42596274457331873 1103 66-67 -2.7335194099315814 1103 68-69 -7.6885357544532695 1103 70-71 -11.643321378508666 1103 72-73 -9.298645994442513 1103 74-75 -7.480216281046925 1103 76-77 -4.06126357430421 1103 78-79 -1.205071458377418 1103 80-81 -0.5092585640682081 1103 82-83 -0.20636754790775313 1103 84-85 -0.2783968085381794 1103 86-87 -0.09879099676633629 1103 88-89 0.057008076435888455 1103 90-91 -0.052792303377927396 1103 92-93 -0.040189412086732546 1103 94-95 0.8862680396027436 1103 96-97 0.9252538599541431 1103 98-99 0.6167800128187579 1103 100-101 0.3218886352796595 1104 1 0.337545178231057 1104 2 0.21480757942198636 1104 3 0.2718333417029406 1104 4 0.13839003571587227 1104 5 0.4628135750263098 1104 6 0.43211285888330764 1104 7 0.5356212440633854 1104 8 0.3681188897427319 1104 9 0.6215499709744918 1104 10-11 0.5549816107445551 1104 12-13 0.5599783550414799 1104 14-15 0.7058674484899896 1104 16-17 0.8010269535639196 1104 18-19 0.7594723466824504 1104 20-21 0.8143744757405997 1104 22-23 0.663212244877279 1104 24-25 0.7598040567697595 1104 26-27 0.9843626363650557 1104 28-29 0.9525964338398012 1104 30-31 0.9485169790160626 1104 32-33 0.6513771534761261 1104 34-35 0.8167809082929764 1104 36-37 0.7872549826149324 1104 38-39 0.8019584184973567 1104 40-41 0.8806756569380667 1104 42-43 0.8865267578029119 1104 44-45 0.6486903335361447 1104 46-47 0.5264162189626234 1104 48-49 0.6181142524715568 1104 50-51 0.6746363032096383 1104 52-53 0.7105966779675512 1104 54-55 0.6620316027321991 1104 56-57 0.5790042684296921 1104 58-59 0.6393017399195564 1104 60-61 0.5037094015218884 1104 62-63 0.5799908719541449 1104 64-65 0.36751162560983275 1104 66-67 -2.8979134090928227 1104 68-69 -7.285709764382698 1104 70-71 -8.971887404205589 1104 72-73 -8.263289371111547 1104 74-75 -6.911528642724935 1104 76-77 -3.5342398344789636 1104 78-79 -0.7117842704366737 1104 80-81 0.14519078465061241 1104 82-83 0.2998689659144098 1104 84-85 0.2503724354330963 1104 86-87 0.4632827915710891 1104 88-89 0.47425207954177395 1104 90-91 0.31243899798027286 1104 92-93 0.3819476773763668 1104 94-95 1.3739104100420114 1104 96-97 1.6606156043994638 1104 98-99 1.3656229177916366 1104 100-101 1.381693216324063 1105 1 0.5542044832344857 1105 2 0.38741601116548097 1105 3 0.4638616327136553 1105 4 0.38517208603631303 1105 5 0.48040169969121393 1105 6 0.5002596365582974 1105 7 0.5280640800538663 1105 8 0.52492440015601 1105 9 0.7257647315259774 1105 10-11 0.9136166680960827 1105 12-13 0.8233705052860785 1105 14-15 1.0373315444082962 1105 16-17 1.010256881919652 1105 18-19 1.0118445732105528 1105 20-21 1.114401318031554 1105 22-23 1.1083051754581845 1105 24-25 1.0495908949492971 1105 26-27 1.1566226044737746 1105 28-29 1.1706648723060198 1105 30-31 1.1053429770770293 1105 32-33 1.1569192357989664 1105 34-35 1.1966616562935215 1105 36-37 1.0697435303671199 1105 38-39 0.9179445480126276 1105 40-41 1.0074749238275231 1105 42-43 1.110968590940395 1105 44-45 0.995447416706277 1105 46-47 0.9598836248513933 1105 48-49 0.7286655993781324 1105 50-51 0.9052077627357704 1105 52-53 1.1742710368777978 1105 54-55 0.9930427132877213 1105 56-57 1.088584848084274 1105 58-59 1.0584836993998543 1105 60-61 1.346332320463027 1105 62-63 1.4056843961906829 1105 64-65 1.201140467217396 1105 66-67 1.7720644434127806 1105 68-69 -5.007855939234702 1105 70-71 -5.178218504038075 1105 72-73 -1.4311167151067892 1105 74-75 1.232780444345483 1105 76-77 1.3336143170824037 1105 78-79 1.2509792562722737 1105 80-81 1.2014038547975439 1105 82-83 1.280750170893672 1105 84-85 1.1433243026564703 1105 86-87 1.3350302906890796 1105 88-89 1.350537071749521 1105 90-91 1.3447044387216636 1105 92-93 1.4322745421690044 1105 94-95 2.395974912101888 1105 96-97 2.410624111981978 1105 98-99 2.005937260811809 1105 100-101 1.884724867309 1106 1 0.21050237774669256 1106 2 0.13314205386278744 1106 3 0.14553513106047333 1106 4 0.08902186944572321 1106 5 0.2577117914546747 1106 6 0.33977889690767427 1106 7 0.32255834171968445 1106 8 0.2872713109339955 1106 9 0.36373487722229925 1106 10-11 0.4084844203485929 1106 12-13 0.43159980446378654 1106 14-15 0.49758204396623995 1106 16-17 0.32786938292431245 1106 18-19 0.3847328842473914 1106 20-21 0.42072959481298966 1106 22-23 0.3744145336128284 1106 24-25 0.4647115995130733 1106 26-27 0.607143675980538 1106 28-29 0.6790977707952877 1106 30-31 0.5801759265613953 1106 32-33 0.5376603414502767 1106 34-35 0.6541470922046031 1106 36-37 0.5716991507751388 1106 38-39 0.5581135108444073 1106 40-41 0.6365855374592897 1106 42-43 0.6610858242346538 1106 44-45 0.6685700557579892 1106 46-47 0.7268143782208085 1106 48-49 0.9060561331360546 1106 50-51 0.7191258906614024 1106 52-53 0.8026747517597741 1106 54-55 0.7519036310886094 1106 56-57 0.6884801557038784 1106 58-59 0.9854316636145946 1106 60-61 0.81810729628193 1106 62-63 0.7581682592910219 1106 64-65 0.7873533606625891 1106 66-67 1.5496885776740044 1106 68-69 -5.27413383757154 1106 70-71 -6.432998255104398 1106 72-73 -2.451574513892332 1106 74-75 0.45079938181828894 1106 76-77 0.8231085302474526 1106 78-79 0.7205730830940311 1106 80-81 0.7260846850753246 1106 82-83 0.8731922956154854 1106 84-85 0.8616562433247843 1106 86-87 0.7234828458483129 1106 88-89 0.8351725468769402 1106 90-91 0.7207993436462914 1106 92-93 0.6756398097962624 1106 94-95 1.6840022887089958 1106 96-97 1.7524352070185465 1106 98-99 1.5420796895641722 1106 100-101 1.4938257946437545 1107 1 0.004437193779246229 1107 2 -0.015944964541358075 1107 3 -0.016707676504211122 1107 4 -0.05318218698134558 1107 5 0.09198406031721618 1107 6 0.1369155988242241 1107 7 0.1412927931517558 1107 8 -0.07368616009782158 1107 9 0.12319901602990058 1107 10-11 0.20359015942239012 1107 12-13 0.38999767015517506 1107 14-15 0.2754797612655224 1107 16-17 0.08268146422768297 1107 18-19 0.21451074019635996 1107 20-21 0.17520858693093544 1107 22-23 0.2163717339435891 1107 24-25 0.39745913059963556 1107 26-27 0.43319551523205035 1107 28-29 0.396517066281703 1107 30-31 0.30840176314396217 1107 32-33 0.3787199633570424 1107 34-35 0.5715807158131199 1107 36-37 0.47600148225844663 1107 38-39 0.4491287561073136 1107 40-41 0.5421747563669115 1107 42-43 0.6488519342957773 1107 44-45 0.3765650421489184 1107 46-47 0.2251472580358893 1107 48-49 0.5154120443940471 1107 50-51 0.43120271759390505 1107 52-53 0.584571037304265 1107 54-55 0.5966684736484495 1107 56-57 0.5453875089736968 1107 58-59 0.5770034321736581 1107 60-61 0.5567160867346388 1107 62-63 0.6346579962668812 1107 64-65 0.5827852275340639 1107 66-67 1.3962837364958602 1107 68-69 -5.634055031137596 1107 70-71 -6.034718228964831 1107 72-73 -2.0106039096297756 1107 74-75 0.5214790564694631 1107 76-77 0.48345167202924344 1107 78-79 0.537093636987862 1107 80-81 0.582436008514442 1107 82-83 0.6384521862494381 1107 84-85 0.5509351422482531 1107 86-87 0.6536540466401135 1107 88-89 0.6060947774254117 1107 90-91 0.6189863162991429 1107 92-93 0.7962573077994897 1107 94-95 1.5816820493641472 1107 96-97 1.7603036018952452 1107 98-99 1.528600239933251 1107 100-101 1.2567114114650089 1108 1 0.04370723943099364 1108 2 -0.00963215033578635 1108 3 0.04705461724848803 1108 4 0.03871437686724022 1108 5 0.03314393184364661 1108 6 0.1889095798545668 1108 7 0.22515258301881858 1108 8 0.09187712447429419 1108 9 0.2162167816058158 1108 10-11 0.20817811689063603 1108 12-13 0.1613893021512922 1108 14-15 0.17300757424404622 1108 16-17 0.11701400656067307 1108 18-19 0.2118804009440396 1108 20-21 0.29834111333389046 1108 22-23 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0.5527504794370728 2316 94-95 1.1538614558940203 2316 96-97 1.184577325049073 2316 98-99 0.010719930333376482 2316 100-101 -0.19114176924368564 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 32.0 4 83.0 5 234.0 6 568.0 7 1435.0 8 3003.0 9 4841.0 10 7671.0 11 11245.0 12 14710.0 13 18647.0 14 22641.0 15 27839.0 16 33453.0 17 38046.0 18 35643.0 19 31256.0 20 30900.0 21 31212.0 22 33073.0 23 38083.0 24 45873.0 25 57290.0 26 71816.0 27 89940.0 28 109798.0 29 131218.0 30 154032.0 31 179927.0 32 208444.0 33 242091.0 34 283023.0 35 327449.0 36 348861.0 37 298484.0 38 126715.0 39 7890.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.41191983965061 20.35451902192609 15.441487438221497 22.792073700201808 2 15.898784448241232 21.249201152948235 38.985529456262306 23.866484942548226 3 19.324792972980372 25.020358770767086 30.252546557851833 25.402301698400702 4 13.349355202358684 17.649375349228713 36.48431901949291 32.516950428919685 5 13.588014610095362 37.83866693963881 33.94441278605026 14.62890566421557 6 31.68770134644988 36.632779935543056 17.072809745086673 14.60670897292039 7 28.03218543987659 33.22724169789214 20.431077818426495 18.309495043804773 8 26.24726403548979 35.44375600249586 19.374997147490085 18.933982814524263 9 26.136130249188742 16.81337804330208 20.38012384131047 36.6703678661987 10-11 24.86120501754864 27.275015207952862 26.8242371410483 21.039542633450196 12-13 25.77982731067973 24.501348666687967 27.721979061599217 21.996844961033084 14-15 22.712417448363052 25.839241017201775 26.344689474140615 25.10365206029456 16-17 21.717044527107614 29.11566981046423 27.00560739254535 22.161678269882803 18-19 21.608544853569935 27.23020880298438 29.897500747366614 21.263745596079072 20-21 22.446538241738395 26.397982965131234 29.96037780935643 21.19510098377394 22-23 22.038312305173573 26.4768340334751 29.80427869967276 21.680574961678566 24-25 21.906999048728693 26.593265338190314 29.96253594977496 21.53719966330603 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27.437318133417833 28.525246189877766 21.980039831575677 52-53 22.307902114488606 27.407685448817016 28.35512877105443 21.929283665639943 54-55 22.007073984878275 27.333097914422233 28.4451342091678 22.214693891531688 56-57 22.04177665837934 27.671504318110628 28.26181922066758 22.024899802842455 58-59 22.114461792080405 27.37136119030117 28.368577188991743 22.14559982862668 60-61 22.142906513024222 27.40197273872525 28.49943662118774 21.95568412706279 62-63 22.192273047796863 27.285023663216535 28.519546889420717 22.00315639956589 64-65 21.979022235204866 27.22170295268576 28.554452501725436 22.244822310383938 66-67 21.504260991390282 27.44413029468877 28.906451666233174 22.145157047687782 68-69 21.592017187439918 27.039422720772794 29.344950696538945 22.023609395248343 70-71 21.141471986557836 26.962779775045092 29.625093791519504 22.27065444687757 72-73 21.885845217577785 27.315253720882183 28.406607884007336 22.392293177532697 74-75 21.686239357915387 27.450599489339915 28.32775882060734 22.535402332137352 76-77 21.881978446270388 27.54954054944958 28.117923739327228 22.450557264952806 78-79 21.813667196410545 27.588272125881357 28.122178315081918 22.475882362626177 80-81 21.711521277265096 27.63056618425398 28.01768190885592 22.640230629625005 82-83 21.83788680849455 27.671008784426537 27.866658364504477 22.624446042574444 84-85 21.845710835690866 27.600022037676602 27.74316913525591 22.81109799137662 86-87 21.772228859986992 27.52416082987092 27.98499738383323 22.718612926308857 88-89 21.87463935889503 27.48156940576778 27.81062079496547 22.833170440371724 90-91 21.64851725219957 27.671232206822637 27.940029911691234 22.74022062928656 92-93 21.707410700677848 27.576197931651087 27.891853828417833 22.824537539253235 94-95 21.32280173150329 27.43695627101444 28.16749344021109 23.07274855727118 96-97 21.260224324948677 27.315272714559296 28.224462835949105 23.200040124542923 98-99 21.531436977855854 27.24776095265763 27.763975114776752 23.45682695470977 100-101 21.421120590653153 27.17857715147631 27.920512472334735 23.4797897855358 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4109.0 1 2803.0 2 1489.5 3 1578.0 4 1523.5 5 1506.5 6 2431.0 7 4614.0 8 6679.5 9 7534.0 10 6896.0 11 5582.0 12 5079.0 13 4926.0 14 4803.0 15 4889.5 16 5012.0 17 5245.0 18 5581.0 19 6071.5 20 6780.0 21 7634.0 22 8891.0 23 10665.0 24 12744.5 25 15450.5 26 19055.0 27 23360.5 28 27602.5 29 31748.5 30 36636.5 31 42144.0 32 47774.0 33 53513.0 34 59622.0 35 65334.5 36 72040.5 37 78597.0 38 82049.0 39 84535.5 40 88238.0 41 93381.0 42 98038.5 43 101533.0 44 105322.0 45 109321.0 46 111644.0 47 113202.5 48 117814.5 49 118546.5 50 114241.5 51 110750.0 52 106957.0 53 104267.0 54 100655.0 55 93328.5 56 84929.0 57 76967.0 58 69671.0 59 61386.5 60 51386.5 61 41340.0 62 32144.0 63 24424.5 64 18131.5 65 13278.0 66 9600.0 67 7080.0 68 5012.5 69 3383.5 70 2442.5 71 1840.0 72 1404.5 73 1100.5 74 804.0 75 552.0 76 362.0 77 208.0 78 121.5 79 73.0 80 41.0 81 23.5 82 20.5 83 13.5 84 9.5 85 4.0 86 2.0 87 1.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.021874676036373903 2 0.01806046277816862 3 0.0 4 0.0 5 1.9560067990796336E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 1.7930062324896643E-4 18-19 0.001206204192765774 20-21 3.260011331799389E-5 22-23 0.0 24-25 0.0 26-27 5.053017564289054E-4 28-29 0.018728765101187494 30-31 0.004286914901316197 32-33 0.008035927932885495 34-35 0.009339932465605251 36-37 0.10106035128578109 38-39 0.10344015955799461 40-41 0.11868071253415677 42-43 0.10360316012458458 44-45 0.09780033995398169 46-47 0.09962594629978935 48-49 0.10189165417538991 50-51 0.10011494799955925 52-53 0.07768607003677945 54-55 0.06573812850573468 56-57 0.03731082969244401 58-59 0.015827355015886033 60-61 0.008671630142586376 62-63 0.001793006232489664 64-65 0.0042706148446572 66-67 0.4187321555129726 68-69 0.8080590088131147 70-71 0.8960793147716981 72-73 0.277818165695944 74-75 0.14916181848648105 76-77 0.014376649973235308 78-79 0.008753130425881361 80-81 0.0014670050993097251 82-83 0.0 84-85 0.0 86-87 2.9340101986194503E-4 88-89 6.520022663598779E-4 90-91 0.003634912634956319 92-93 0.0016789058358766856 94-95 0.2210613684093166 96-97 0.22829859356591123 98-99 0.014115849066691355 100-101 0.007351325553207623 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3067474.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.966704545063166 #Duplication Level Percentage of deduplicated Percentage of total 1 70.21201292427553 35.082629053014315 2 15.397671128299848 15.38741767899616 3 6.01918868747651 9.022770682443761 4 2.915951886245915 5.828020254706772 5 1.5780834597180504 3.9425814989591452 6 0.9733159473876447 2.918003422327001 7 0.6211928128076925 2.172727042015509 8 0.4136637359665706 1.6535530940838923 9 0.3166811428182479 1.4241161788373111 >10 1.4089621997674506 12.427607240722798 >50 0.09258292152680254 3.1836764009160627 >100 0.04740100388403972 4.2127109592015755 >500 0.002502033867720825 0.7606052867200914 >1k 5.925869683554227E-4 0.6699011566719527 >5k 0.0 0.0 >10k+ 1.9752898945180758E-4 1.3136800503836101 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16033 0.5226776168273961 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13864 0.4519679710406673 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10033 0.32707693691943274 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4913 0.160164356731304 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3756 0.12244602562238506 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3120 0.10171235355214095 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 3.260011331799389E-5 0.0 0.0 0.0 0.0 7 3.260011331799389E-5 0.0 0.0 0.0 0.0 8 3.260011331799389E-5 0.0 0.0 0.0 0.0 9 3.260011331799389E-5 0.0 0.0 0.0 0.0 10-11 3.260011331799389E-5 0.0 0.0 1.6300056658996945E-5 0.0 12-13 4.890016997699084E-5 0.0 0.0 3.260011331799389E-5 0.0 14-15 6.520022663598778E-5 0.0 0.0 3.260011331799389E-5 0.0 16-17 6.520022663598778E-5 0.0 0.0 3.260011331799389E-5 0.0 18-19 9.780033995398168E-5 0.0 0.0 6.520022663598778E-5 0.0 20-21 9.780033995398168E-5 0.0 0.0 2.119007365669603E-4 0.0 22-23 9.780033995398168E-5 0.0 0.0 3.260011331799389E-4 0.0 24-25 9.780033995398168E-5 0.0 0.0 7.824027196318534E-4 0.0 26-27 9.780033995398168E-5 0.0 0.0 0.0014670050993097253 0.0 28-29 9.780033995398168E-5 0.0 0.0 0.0033578116717533713 0.0 30-31 1.3040045327197556E-4 0.0 0.0 0.007889227422954522 0.0 32-33 1.6300056658996947E-4 0.0 0.0 0.01660975773551789 0.0 34-35 1.793006232489664E-4 0.0 0.0 0.031198308445320157 0.0 36-37 1.9560067990796336E-4 0.0 0.0 0.05452368952434479 0.0 38-39 1.9560067990796336E-4 0.0 0.0 0.0875476043154726 0.0 40-41 1.9560067990796336E-4 0.0 0.0 0.1244183324781237 0.0 42-43 2.2820079322595724E-4 0.0 0.0 0.16164766188727273 0.0 44-45 2.608009065439511E-4 0.0 0.0 0.20621201679297035 0.0 46-47 3.586012464979328E-4 0.0 0.0 0.2624309122098508 0.0 48-49 3.586012464979328E-4 0.0 0.0 0.3237191252476794 0.0 50-51 3.586012464979328E-4 0.0 0.0 0.3885770506938282 0.0 52-53 3.586012464979328E-4 0.0 0.0 0.45407067834967796 0.0 54-55 3.586012464979328E-4 1.6300056658996945E-5 0.0 0.5265081301422604 0.0 56-57 3.912013598159267E-4 3.260011331799389E-5 0.0 0.6092146176300108 1.6300056658996945E-5 58-59 3.912013598159267E-4 3.260011331799389E-5 0.0 0.6915136037012865 3.260011331799389E-5 60-61 3.912013598159267E-4 3.260011331799389E-5 0.0 0.7729975869396122 3.260011331799389E-5 62-63 3.912013598159267E-4 3.260011331799389E-5 0.0 0.858964085759162 3.260011331799389E-5 64-65 3.912013598159267E-4 3.260011331799389E-5 0.0 0.950472603842771 3.260011331799389E-5 66-67 4.238014731339206E-4 3.260011331799389E-5 0.0 1.0457464350146082 3.260011331799389E-5 68-69 4.238014731339206E-4 3.260011331799389E-5 0.0 1.157923424941825 3.260011331799389E-5 70-71 4.238014731339206E-4 3.260011331799389E-5 0.0 1.3071504436549422 3.260011331799389E-5 72-73 4.401015297929176E-4 3.260011331799389E-5 0.0 1.4750247271859518 3.260011331799389E-5 74-75 4.5640158645191453E-4 3.260011331799389E-5 0.0 1.654569851284803 3.260011331799389E-5 76-77 4.5640158645191453E-4 3.260011331799389E-5 0.0 1.8546693468306494 3.260011331799389E-5 78-79 4.5640158645191453E-4 3.260011331799389E-5 0.0 2.0910853686127413 3.260011331799389E-5 80-81 4.5640158645191453E-4 3.260011331799389E-5 0.0 2.3454966529463657 3.260011331799389E-5 82-83 4.5640158645191453E-4 4.890016997699084E-5 0.0 2.610160672918499 3.260011331799389E-5 84-85 4.890016997699084E-4 9.780033995398168E-5 0.0 2.872542684958373 3.260011331799389E-5 86-87 5.216018130879022E-4 9.780033995398168E-5 0.0 3.150295650427681 3.260011331799389E-5 88-89 5.216018130879022E-4 9.780033995398168E-5 0.0 3.453509304398342 3.260011331799389E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8755 0.0 45.58354 1 GTATCAA 14370 0.0 36.5456 1 ATCAACG 18315 0.0 28.324886 3 TATCAAC 18520 0.0 28.041964 2 TCAACGC 18560 0.0 27.874409 4 CAACGCA 19030 0.0 27.18597 5 AACGCAG 19445 0.0 26.630125 6 ACGCAGA 22125 0.0 23.275942 7 CGCAGAG 22400 0.0 22.820988 8 GTACATG 14305 0.0 21.768559 1 GAGTACT 13105 0.0 21.474573 12-13 TACATGG 14125 0.0 21.201544 2 GCAGAGT 23445 0.0 21.056126 9 GTGGTAT 4970 0.0 20.980625 1 TGGTATC 4945 0.0 20.602077 2 ACATGGG 14320 0.0 20.21432 3 GTACTTT 14055 0.0 20.073635 14-15 AGTACTT 13600 0.0 20.048485 12-13 AGAGTAC 21805 0.0 18.83753 10-11 CAGAGTA 23155 0.0 18.772507 10-11 >>END_MODULE