FastQCFastQC Report
Fri 27 May 2016
SRR939420_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939420_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3043628
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114030.37465156714289655No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80780.26540694197845466No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60680.1993673339843108No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32060.10533481752697767No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA131850.042.52951
GGTATCA75550.042.441441
TATCAAC167850.033.0680542
ATCAACG172700.032.4594153
TCAACGC179050.031.7669324
CAACGCA178950.031.3350855
AACGCAG182150.030.4344026
ACGCAGA204800.026.4996727
CGCAGAG208100.026.0565818
GTACATG130150.025.1780451
TACATGG129450.024.1397572
GCAGAGT207100.023.863929
GAGTACT115000.022.1375612-13
ACATGGG137900.021.7734573
AGTACTT120800.020.5572512-13
GTACTTT126850.020.44097514-15
GTGGTAT45900.019.849571
AGAGTAC190650.019.6142910-11
CAGAGTA202700.019.15413910-11
TGGTATC47250.019.1471442
Produced by FastQC (version 0.11.5)