FastQCFastQC Report
Fri 27 May 2016
SRR939420_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939420_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3043628
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156710.5148789536697651No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134360.44144685224344105No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97800.3213270478521028No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA47190.1550452289175944No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35040.11512576438382088No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA84500.043.815491
GTATCAA137500.036.0747531
ATCAACG176950.027.8887923
TATCAAC179700.027.4387512
TCAACGC180100.027.328264
CAACGCA184150.026.6240735
AACGCAG190850.025.7142496
ACGCAGA219500.022.2067137
GTGGTAT48850.022.0572361
CGCAGAG220800.021.9685928
TGGTATC49100.021.8435822
GTACATG139100.021.225261
TACATGG139000.020.7580092
GAGTACT130500.020.43326612-13
GCAGAGT232650.020.1159139
AGTACTT135750.019.6779512-13
ACATGGG140400.019.433353
GTACTTT140100.019.01620914-15
AGAGTAC216050.018.26861810-11
GTATAGG7400.017.9604031
Produced by FastQC (version 0.11.5)