##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939420_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3043628 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.887364027404136 31.0 31.0 31.0 28.0 34.0 2 31.14376724093746 31.0 31.0 34.0 28.0 34.0 3 31.1465862450996 31.0 31.0 34.0 28.0 34.0 4 35.053657345772876 35.0 35.0 37.0 32.0 37.0 5 34.61798156673549 35.0 35.0 37.0 30.0 37.0 6 34.68121301289119 35.0 35.0 37.0 31.0 37.0 7 34.52294629961349 35.0 35.0 37.0 30.0 37.0 8 34.416351472650405 35.0 35.0 37.0 30.0 37.0 9 35.93230283070073 37.0 35.0 39.0 30.0 39.0 10-11 35.73394136865609 37.0 35.0 39.0 30.0 39.0 12-13 35.71285764883225 37.0 35.0 39.0 30.0 39.0 14-15 36.54918373730298 38.0 35.0 40.0 30.5 41.0 16-17 36.46308698040628 38.0 35.0 40.0 30.0 41.0 18-19 36.46473912054955 38.0 35.0 40.0 30.0 41.0 20-21 36.34171258774069 38.0 34.5 40.0 29.5 41.0 22-23 36.182106354653065 38.0 34.0 40.0 29.0 41.0 24-25 36.01635909513252 38.0 34.0 40.0 29.0 41.0 26-27 35.544830544337216 38.0 34.0 40.0 27.0 41.0 28-29 35.33516382422556 38.0 34.0 40.0 27.0 41.0 30-31 35.03781030401876 37.5 33.0 40.0 27.0 41.0 32-33 34.797165422318365 37.0 33.0 40.0 25.5 41.0 34-35 35.048909886490726 38.0 33.0 40.0 27.0 41.0 36-37 34.900514287554195 38.0 33.0 40.0 25.5 41.0 38-39 34.72758382430442 38.0 33.0 40.0 25.0 41.0 40-41 34.56893138714718 37.5 33.0 40.0 25.0 41.0 42-43 34.40501878021887 37.0 33.0 40.0 24.5 41.0 44-45 34.02131255856497 37.0 33.0 40.0 24.0 41.0 46-47 33.62955377595422 37.0 32.0 40.0 23.0 41.0 48-49 33.2457782291397 37.0 31.5 40.0 21.5 41.0 50-51 33.0097158719791 37.0 31.0 39.0 20.5 40.5 52-53 32.89707053555822 36.5 31.0 39.0 20.0 40.0 54-55 32.56383648067373 36.0 31.0 39.0 18.5 40.0 56-57 32.071617162149906 35.5 30.0 39.0 16.5 40.0 58-59 31.611770558031402 35.0 29.5 39.0 14.0 40.0 60-61 31.245575346264392 35.0 29.0 38.0 13.0 40.0 62-63 31.08084118689932 35.0 29.0 38.0 12.0 40.0 64-65 30.620463801752383 34.0 28.0 38.0 8.0 40.0 66-67 29.762489042681956 34.0 27.0 37.5 7.0 39.5 68-69 26.325782585782495 31.0 19.0 36.0 2.0 39.0 70-71 25.01779504591231 30.0 13.5 35.0 2.0 38.0 72-73 26.030083998438705 31.0 19.5 35.0 2.0 38.0 74-75 26.77697471570113 31.0 22.5 35.0 2.0 37.5 76-77 26.43993171964511 30.5 23.0 34.0 2.0 36.0 78-79 27.54688976445216 32.0 26.0 35.0 2.0 37.0 80-81 27.569863662707796 32.0 26.0 35.0 2.0 36.5 82-83 27.247236193122156 32.0 26.0 35.0 2.0 36.0 84-85 26.889483865965225 32.0 25.0 35.0 2.0 36.0 86-87 26.435449897293623 32.0 24.5 34.0 2.0 35.0 88-89 25.949689975253218 31.5 23.5 34.0 2.0 35.0 90-91 25.352885602314082 31.0 21.0 34.0 2.0 35.0 92-93 24.78906012824169 31.0 19.5 34.0 2.0 35.0 94-95 23.947628783806692 31.0 13.5 34.0 2.0 35.0 96-97 23.277118787184243 30.0 4.5 34.0 2.0 35.0 98-99 22.41523767030662 30.0 2.0 34.0 2.0 35.0 100-101 20.998467125417427 28.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.30084508309843727 1101 2 0.21345472169045365 1101 3 0.28605700805475465 1101 4 0.3108975974702517 1101 5 0.46924159090697515 1101 6 0.3614785806177423 1101 7 0.5149793470128898 1101 8 0.6680452074567995 1101 9 0.6857219338064269 1101 10-11 0.7564900249503097 1101 12-13 0.6744306628241432 1101 14-15 0.6950739131953654 1101 16-17 0.61006412459313 1101 18-19 0.4440823543868433 1101 20-21 0.6168303861954172 1101 22-23 0.7120822740393677 1101 24-25 0.6956657355215938 1101 26-27 0.7913749449460994 1101 28-29 0.7618506065106345 1101 30-31 0.6231545822678228 1101 32-33 0.9885893986704417 1101 34-35 0.7532436928612256 1101 36-37 0.8457588979912387 1101 38-39 0.8179707854829346 1101 40-41 0.7471601859825228 1101 42-43 0.41369629708735545 1101 44-45 0.6295067051706269 1101 46-47 0.7206739720131239 1101 48-49 0.7049568593002533 1101 50-51 0.41768773537850024 1101 52-53 0.2915365162417203 1101 54-55 0.4296047732011985 1101 56-57 0.40085779263040067 1101 58-59 0.5126090995418089 1101 60-61 0.4952952345819881 1101 62-63 0.13654460561149406 1101 64-65 -0.007465093154184643 1101 66-67 -0.02031124870548595 1101 68-69 -6.976890275844195 1101 70-71 -7.978772529593549 1101 72-73 -4.912848887950801 1101 74-75 -2.973842556727895 1101 76-77 -0.21595341580421845 1101 78-79 0.0030192756602289705 1101 80-81 -0.1410553850619891 1101 82-83 -0.3228573611455836 1101 84-85 -0.38927827157332473 1101 86-87 -0.49252761448693505 1101 88-89 -0.5276351983093619 1101 90-91 -0.6546336052602904 1101 92-93 -0.46238240318231405 1101 94-95 -0.48167582896305916 1101 96-97 -0.5557311200914263 1101 98-99 -0.6523185377528158 1101 100-101 -0.6476505817490086 1102 1 0.26798299466555164 1102 2 0.24118258058547326 1102 3 0.22113830333713835 1102 4 0.2522339128284159 1102 5 0.43212659145163457 1102 6 0.3023059388273026 1102 7 0.456563722480432 1102 8 0.49914502046250675 1102 9 0.4721796960131499 1102 10-11 0.49049075810172127 1102 12-13 0.49986884735681514 1102 14-15 0.6813689085555339 1102 16-17 0.557376618406316 1102 18-19 0.5084456358857992 1102 20-21 0.5073156447442884 1102 22-23 0.6548845758274524 1102 24-25 0.4921928939903282 1102 26-27 0.6025172192613439 1102 28-29 0.5108822093143246 1102 30-31 0.214152866006458 1102 32-33 0.5121554706648297 1102 34-35 0.37224384066672656 1102 36-37 0.5639507712202274 1102 38-39 0.6780496060402896 1102 40-41 0.5462962696431362 1102 42-43 0.4095954404030877 1102 44-45 0.4590235529630604 1102 46-47 0.5713553011054557 1102 48-49 0.5669824183508112 1102 50-51 0.3356329949127499 1102 52-53 0.1882362500562209 1102 54-55 0.12167579939170281 1102 56-57 0.30982154050768074 1102 58-59 0.30778566834798227 1102 60-61 0.39765899249376346 1102 62-63 0.20024321438662085 1102 64-65 0.08426611599872658 1102 66-67 0.07331200638736135 1102 68-69 -6.953669942147297 1102 70-71 -7.849272767573957 1102 72-73 -1.7330586903931646 1102 74-75 -0.13029476379842464 1102 76-77 0.38497097874695285 1102 78-79 0.18030062183832385 1102 80-81 0.004174271681218755 1102 82-83 -0.15254330384857795 1102 84-85 -0.38269762740352675 1102 86-87 -0.3344144262139217 1102 88-89 -0.35739007170966275 1102 90-91 -0.4447104776779298 1102 92-93 -0.36151482560590154 1102 94-95 -0.2930513828381649 1102 96-97 -0.4451087866358989 1102 98-99 -0.6619847007357222 1102 100-101 -0.6549010062943026 1103 1 0.36691274492227777 1103 2 0.18545820018880477 1103 3 0.28192269777126455 1103 4 0.3006478858478232 1103 5 0.3120753632450075 1103 6 0.4273704309416999 1103 7 0.3462514245172841 1103 8 0.4700156977166401 1103 9 0.42070150481558954 1103 10-11 0.39412383753542457 1103 12-13 0.3848262701419287 1103 14-15 0.46688033368400284 1103 16-17 0.47073288207207753 1103 18-19 0.5233982257542706 1103 20-21 0.48045308231911577 1103 22-23 0.47457899497970857 1103 24-25 0.5662015428530296 1103 26-27 0.5892786825350811 1103 28-29 0.5433271985165575 1103 30-31 0.5367106640700285 1103 32-33 0.7526373394209784 1103 34-35 0.5633506530920798 1103 36-37 0.647921100944366 1103 38-39 0.7159891104853671 1103 40-41 0.612454933018789 1103 42-43 0.36631258242483966 1103 44-45 0.6019946310441213 1103 46-47 0.44591019906420115 1103 48-49 0.5930356357887661 1103 50-51 0.3911272174128442 1103 52-53 0.33685451315849946 1103 54-55 0.17063582634532537 1103 56-57 0.3751607903197858 1103 58-59 0.5735307039632467 1103 60-61 0.39206401326253015 1103 62-63 0.23765768914935492 1103 64-65 0.15725774994325903 1103 66-67 -0.06008691610642458 1103 68-69 -6.869756164999398 1103 70-71 -7.776975155716066 1103 72-73 -1.3494917956591976 1103 74-75 0.25679333828470163 1103 76-77 0.5611222738763537 1103 78-79 0.29608370513978244 1103 80-81 0.009590544771523213 1103 82-83 0.005842675867359759 1103 84-85 -0.18766362666404035 1103 86-87 -0.3027095439036067 1103 88-89 -0.2659173663337704 1103 90-91 -0.5222348338745313 1103 92-93 -0.556605778116289 1103 94-95 -0.09428300480330876 1103 96-97 -0.11336787035464724 1103 98-99 0.08438604322827814 1103 100-101 0.10905940743458942 1104 1 0.32859673525312516 1104 2 0.36045543577430905 1104 3 0.29706958965956076 1104 4 0.1879150626597763 1104 5 0.4814064239263516 1104 6 0.3184297545769539 1104 7 0.5149862114894006 1104 8 0.5384713488097432 1104 9 0.5922404228727416 1104 10-11 0.605701100471137 1104 12-13 0.35925263663749973 1104 14-15 0.5111850624424576 1104 16-17 0.5059713810579964 1104 18-19 0.49523514069569075 1104 20-21 0.4593284940069253 1104 22-23 0.5389706068363225 1104 24-25 0.5445860960046716 1104 26-27 0.6528847107782951 1104 28-29 0.6360107556391554 1104 30-31 0.46938772306892673 1104 32-33 0.6144482828631581 1104 34-35 0.3769362106709835 1104 36-37 0.6469427081679555 1104 38-39 0.6401770549787216 1104 40-41 0.7842112425324359 1104 42-43 0.6166344267582105 1104 44-45 0.6861996589192145 1104 46-47 0.6627777290213572 1104 48-49 0.7245731261483073 1104 50-51 0.7379187294705929 1104 52-53 0.601867820839928 1104 54-55 0.6744324347236699 1104 56-57 0.6133255095322561 1104 58-59 0.6897535129722918 1104 60-61 0.7680512416675533 1104 62-63 0.6072368122910774 1104 64-65 0.5416318270137381 1104 66-67 0.5425782769056973 1104 68-69 -6.682740637388147 1104 70-71 -4.247925533426066 1104 72-73 0.3958186725836299 1104 74-75 0.9132484385944544 1104 76-77 1.1827309671551198 1104 78-79 0.5818425439177268 1104 80-81 0.40655626743146556 1104 82-83 0.2827888936584344 1104 84-85 0.3112384808695836 1104 86-87 0.2863975957042264 1104 88-89 0.4103480006615001 1104 90-91 0.3172953661291089 1104 92-93 0.4832726506325038 1104 94-95 0.6820691263153194 1104 96-97 0.2961679774527859 1104 98-99 0.4615596953487575 1104 100-101 0.6725869647979366 1105 1 0.21791190956030704 1105 2 0.21177561804839584 1105 3 0.24904121932121015 1105 4 0.21884991687434763 1105 5 0.3988255590769114 1105 6 0.32563241395600784 1105 7 0.31747766979024306 1105 8 0.46695727441314006 1105 9 0.5654620695427823 1105 10-11 0.46776700718562836 1105 12-13 0.41206167205088917 1105 14-15 0.4103459994632672 1105 16-17 0.5055736464704736 1105 18-19 0.5697973797041698 1105 20-21 0.6009161632578213 1105 22-23 0.5413509960091503 1105 24-25 0.4615125112681895 1105 26-27 0.6700591693795346 1105 28-29 0.6266455016629422 1105 30-31 0.4576135581632812 1105 32-33 0.5469496705730919 1105 34-35 0.4220983625267465 1105 36-37 0.49095286016885353 1105 38-39 0.5458945943413624 1105 40-41 0.568350651296484 1105 42-43 0.5193380799503942 1105 44-45 0.5885355820629314 1105 46-47 0.5201631020876789 1105 48-49 0.6372855609015247 1105 50-51 0.4241474833785972 1105 52-53 0.3130415111854177 1105 54-55 0.3966668971696734 1105 56-57 0.6310166742072845 1105 58-59 0.6238631963092764 1105 60-61 0.6823701403753724 1105 62-63 0.46967385073142864 1105 64-65 0.39053934779100885 1105 66-67 0.5918857651664204 1105 68-69 -3.0200691972086524 1105 70-71 -3.2711348293904905 1105 72-73 0.8805468855377043 1105 74-75 1.246831664382448 1105 76-77 1.0572214569373521 1105 78-79 0.895159796911468 1105 80-81 0.6699951609198855 1105 82-83 0.678037814454477 1105 84-85 0.6262765465118392 1105 86-87 0.632200693358886 1105 88-89 0.39058678445891104 1105 90-91 0.43060087897471533 1105 92-93 0.33730588868398215 1105 94-95 0.5504247345300044 1105 96-97 0.600887796113728 1105 98-99 0.602162535383048 1105 100-101 0.7087556921833276 1106 1 0.0831179104844395 1106 2 0.14189006771639967 1106 3 0.1520055780597005 1106 4 0.0889240382069687 1106 5 0.24982373483441478 1106 6 0.15471936716015477 1106 7 0.1774317316618763 1106 8 0.24129558025565956 1106 9 0.2419419880382847 1106 10-11 0.37523165277247017 1106 12-13 0.22068489178944617 1106 14-15 0.2649581289095977 1106 16-17 0.37335598367696576 1106 18-19 0.35971142764316966 1106 20-21 0.38621714560746057 1106 22-23 0.11047120525300613 1106 24-25 0.3754865302962642 1106 26-27 0.4908292865634465 1106 28-29 0.3823430801258638 1106 30-31 0.4425292911597509 1106 32-33 0.5720587930573302 1106 34-35 0.5223424132494401 1106 36-37 0.7144729946625858 1106 38-39 0.7475305307881968 1106 40-41 0.5774235552898901 1106 42-43 0.5531645487058228 1106 44-45 0.6577056894968365 1106 46-47 0.785491914380053 1106 48-49 0.6922414844613769 1106 50-51 0.7380938243664019 1106 52-53 0.5609310385356281 1106 54-55 0.5043611450829601 1106 56-57 0.5639883913832868 1106 58-59 0.8226544943475425 1106 60-61 0.953535804485842 1106 62-63 0.5892087183502035 1106 64-65 0.5813208823873204 1106 66-67 0.6773588035405105 1106 68-69 -2.8449912853799013 1106 70-71 -3.012466144894006 1106 72-73 0.953756096902552 1106 74-75 1.436787626801074 1106 76-77 1.4087007748882634 1106 78-79 1.071386073633935 1106 80-81 0.8599918367289625 1106 82-83 0.7114571493054207 1106 84-85 0.5626991871841085 1106 86-87 0.7711857871356891 1106 88-89 0.7638936019823781 1106 90-91 0.8800066754310798 1106 92-93 0.6855738827661462 1106 94-95 1.0414221295795407 1106 96-97 0.9435506779364431 1106 98-99 1.0454306778350535 1106 100-101 0.9767985244363864 1107 1 0.027133095208796476 1107 2 0.010917945736775181 1107 3 0.08513441649027698 1107 4 -0.11666769078230743 1107 5 0.3216718492157682 1107 6 0.0795014409790511 1107 7 0.06172754154582094 1107 8 0.10936020684398073 1107 9 0.20214370812749394 1107 10-11 0.025331999919359305 1107 12-13 0.07319488038023536 1107 14-15 0.05715180720539337 1107 16-17 0.10818675694960689 1107 18-19 0.22381041395064472 1107 20-21 0.26988244184094157 1107 22-23 0.14639470895694728 1107 24-25 0.06940808351291139 1107 26-27 0.4791534836755744 1107 28-29 0.3784788201671603 1107 30-31 0.3164095874744177 1107 32-33 0.16528219654326648 1107 34-35 -0.0066205333959104 1107 36-37 0.15410502560470718 1107 38-39 0.30379439563115795 1107 40-41 0.23712689746425042 1107 42-43 0.1137987448881006 1107 44-45 0.271514279758712 1107 46-47 0.538775843486512 1107 48-49 0.3695719035025036 1107 50-51 0.30805085903533325 1107 52-53 0.1282893606885267 1107 54-55 0.3134491760427949 1107 56-57 0.6724187509430344 1107 58-59 0.6621484118972027 1107 60-61 0.6823036839526608 1107 62-63 0.3895878198714797 1107 64-65 0.24775305516964607 1107 66-67 0.507515491948137 1107 68-69 -2.4171125454699656 1107 70-71 -2.5577616551571296 1107 72-73 1.1379705073358508 1107 74-75 1.2117769998555659 1107 76-77 0.8800991628396737 1107 78-79 0.8260777962765609 1107 80-81 0.6350552847595061 1107 82-83 0.5862856606622309 1107 84-85 0.4477523514309354 1107 86-87 0.5496099144346864 1107 88-89 0.4432688778032734 1107 90-91 0.41252116202415223 1107 92-93 0.45180783349549003 1107 94-95 0.635703153987567 1107 96-97 0.5818623108994565 1107 98-99 0.8975720442536073 1107 100-101 1.213489751154885 1108 1 0.06755844870921024 1108 2 0.1300274077033805 1108 3 0.04528346840688613 1108 4 0.133238496654144 1108 5 -0.0493479300364541 1108 6 -0.12201213825849777 1108 7 0.1224709143957341 1108 8 0.23503130733348598 1108 9 0.22528868260418733 1108 10-11 0.21523954999639727 1108 12-13 0.2349450349875326 1108 14-15 0.2527101229816182 1108 16-17 0.31228516056375355 1108 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-5.417815260751805 2316 40-41 -4.879256291630657 2316 42-43 -3.6280302131512983 2316 44-45 -2.0032830643286275 2316 46-47 -2.1933582186304363 2316 48-49 -2.6403062992756148 2316 50-51 -1.030332670751104 2316 52-53 -0.7692819160529751 2316 54-55 -0.02221900521991671 2316 56-57 -3.4261484930476485 2316 58-59 -0.5070447416843145 2316 60-61 -0.3245194045641817 2316 62-63 0.013615275164976737 2316 64-65 0.06375926168055912 2316 66-67 0.054342987235855844 2316 68-69 2.848171357966205 2316 70-71 3.9548263413915166 2316 72-73 2.863605508206099 2316 74-75 1.1537159420083682 2316 76-77 -1.3975061089914327 2316 78-79 0.40496269808704 2316 80-81 0.4960235152743593 2316 82-83 0.5282792245548542 2316 84-85 0.7306596351936498 2316 86-87 0.7770344716282764 2316 88-89 0.5892514951289165 2316 90-91 0.6533678545389385 2316 92-93 0.6008955578612323 2316 94-95 0.9216525909207505 2316 96-97 0.8807163653138197 2316 98-99 1.1859489083362043 2316 100-101 0.9847107599721312 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9.0 3 47.0 4 108.0 5 270.0 6 619.0 7 1523.0 8 3207.0 9 5274.0 10 8168.0 11 11930.0 12 15700.0 13 19722.0 14 24231.0 15 30331.0 16 35770.0 17 39829.0 18 37333.0 19 34326.0 20 33697.0 21 34871.0 22 36921.0 23 41034.0 24 48674.0 25 59922.0 26 75193.0 27 93241.0 28 114290.0 29 135721.0 30 159043.0 31 185254.0 32 213313.0 33 246953.0 34 283095.0 35 317645.0 36 322497.0 37 261317.0 38 106485.0 39 6065.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.175647085036445 20.498009499979297 15.574569973782054 22.7517734412022 2 15.900520002208301 21.36701692767553 38.85734904030937 23.8751140298068 3 19.22062816955551 25.174653084089428 30.364748382263794 25.23997036409127 4 13.330880017048246 17.676308265439612 36.5771699516769 32.41564176583523 5 13.553101758822036 37.920928576028345 33.95250667952851 14.573462985621108 6 31.678181433473473 36.66154339492211 17.06019920962746 14.600075961976957 7 27.884912348026763 33.282713919046614 20.48841054162992 18.3439631912967 8 26.356341839410074 35.3361514613481 19.371092656527015 18.936414042714812 9 26.0744085676699 16.799556318971966 20.48387647899152 36.642158634366616 10-11 24.826150146207045 27.303783704921685 26.878174815585908 20.99189133328536 12-13 25.681045778261996 24.56438500368639 27.763363328238537 21.991205889813077 14-15 22.657088842657515 25.877751814610722 26.370436860220764 25.094722482511006 16-17 21.738429036571702 29.132280417004807 26.959334474522027 22.16995607190146 18-19 21.559676885186867 27.28581125658097 29.955309854305263 21.1992020039269 20-21 22.488917830957003 26.426176260699403 29.974343119461377 21.110562788882213 22-23 22.071592262515914 26.453981683025994 29.808205675797772 21.666220378660313 24-25 21.826082056591353 26.633391615600548 30.01235575303148 21.52817057477662 26-27 21.771735782258165 26.861249953795163 29.76836796300327 21.5986463009434 28-29 21.632684307900966 26.890375145921475 29.937320709202197 21.53961983697536 30-31 21.779312134892002 26.709641808110078 29.85380895118313 21.657237105814787 32-33 21.701104167409344 26.79488192540065 29.722922369538363 21.781091537651644 34-35 22.025437193027646 26.878559491091856 29.600755437220954 21.49524787865954 36-37 21.986284128756424 26.687219702371745 29.641980279674087 21.684515889197755 38-39 21.789196860788117 26.936236218014965 29.747299922271175 21.52726699892574 40-41 22.000789797993217 26.73520460437105 29.691784785636592 21.572220811999145 42-43 21.815584735436918 26.84748934992057 29.463452360264103 21.873473554378414 44-45 21.974412757056992 27.058338427260527 29.221512013502554 21.745736802179927 46-47 22.038821032750477 27.20448054377855 28.734202979181507 22.022495444289465 48-49 22.102143077904397 27.266342695188435 28.693685844112288 21.93782838279488 50-51 22.069936475636553 27.444675311609025 28.526846650993125 21.958541561761297 52-53 22.231644328931864 27.4599054111073 28.368712257353586 21.93973800260725 54-55 21.991506404382786 27.367167793957908 28.472758548202936 22.168567253456366 56-57 22.027194257679124 27.678891709197234 28.291959590661463 22.001954442462175 58-59 22.149550999965985 27.344793017647305 28.349663818916547 22.155992163470156 60-61 22.09087360213669 27.351804801236586 28.579634176476464 21.977687420150264 62-63 22.120479378387163 27.30745327890815 28.526204372403228 22.045862970301457 64-65 21.9680410194739 27.253898353257384 28.479734641724434 22.29832598554428 66-67 21.761686163280597 27.489592199138745 28.50183443505833 22.246887202522323 68-69 21.878433347717543 27.024748563123296 29.04723028944031 22.049587799718847 70-71 21.388139777547078 26.922912465345313 29.432319447593365 22.256628309514245 72-73 21.866152031650977 27.24418356590497 28.54494928662315 22.344715115820904 74-75 21.89029000466795 27.44853748496065 28.096429346289636 22.56474316408176 76-77 21.27228147635572 27.560359604683722 28.6318884644772 22.535470454483356 78-79 21.853028309260196 27.541710733616338 28.09497857758869 22.510282379534775 80-81 21.657042409705852 27.656582416770025 28.027533862872822 22.6588413106513 82-83 21.821662770756706 27.67952440163735 27.864241296382726 22.634571531223216 84-85 21.79199503749559 27.60856340852822 27.76702579441058 22.83241575956561 86-87 21.77985698402143 27.524168938117533 27.985156276695573 22.71081780116547 88-89 21.82344380341203 27.488517353298192 27.81495488341247 22.87308395987731 90-91 21.65451808470948 27.71100607475645 27.854935926491127 22.779539914042942 92-93 21.73777187082614 27.56260483424484 27.85618179100015 22.843441503928872 94-95 21.51065401404029 27.457844352704264 27.889022474956178 23.14247915829927 96-97 21.587879277907714 27.28023606964291 27.91782196962227 23.2140626828271 98-99 21.508979812468066 27.199392747472658 27.73481334706881 23.55681409299047 100-101 21.42420458057358 27.179175094550303 27.908303749825635 23.488316575050487 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4049.0 1 2740.0 2 1468.0 3 1598.0 4 1500.5 5 1499.5 6 2419.0 7 4491.0 8 6596.0 9 7420.0 10 6626.0 11 5380.0 12 4905.5 13 4768.5 14 4767.0 15 4909.5 16 5087.5 17 5303.0 18 5602.5 19 6090.0 20 6741.0 21 7681.0 22 8886.0 23 10428.5 24 12460.0 25 15259.5 26 19047.0 27 23323.5 28 27460.5 29 31547.5 30 36351.0 31 41724.5 32 47452.5 33 53236.5 34 59213.0 35 64948.0 36 71333.5 37 77602.0 38 81467.5 39 84236.5 40 87635.5 41 92711.5 42 97219.5 43 100810.5 44 105008.5 45 108553.0 46 110606.5 47 112124.0 48 116259.5 49 116945.5 50 113030.5 51 109733.0 52 105893.0 53 103243.5 54 99557.0 55 92378.0 56 84220.5 57 76367.5 58 69117.0 59 61169.5 60 51708.5 61 41525.5 62 32043.5 63 24336.0 64 18008.0 65 13236.0 66 9747.5 67 7242.0 68 5157.5 69 3439.5 70 2408.5 71 1805.5 72 1391.5 73 1125.5 74 824.0 75 529.0 76 347.0 77 210.0 78 129.0 79 84.5 80 55.0 81 35.0 82 24.0 83 12.5 84 8.0 85 7.0 86 4.0 87 2.0 88 1.0 89 0.0 90 0.0 91 0.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02214462477017559 2 0.018530516869998567 3 7.556771064006508E-4 4 0.09350682803548922 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.011203734490548779 12-13 0.0 14-15 0.0 16-17 5.913994745744223E-4 18-19 1.3142210546098274E-4 20-21 0.0 22-23 9.856657909573705E-5 24-25 0.0034991135578986655 26-27 0.003794813295185877 28-29 0.0012813655282445818 30-31 2.628442109219655E-4 32-33 7.39249343218028E-4 34-35 0.0030391361887852257 36-37 0.08386373104728961 38-39 0.09621740896062199 40-41 0.0770133538001359 42-43 0.08061103393713029 44-45 0.07967465143572079 46-47 0.07852470801293719 48-49 0.07254500221446247 50-51 0.07216716366126216 52-53 0.056757921795961924 54-55 0.047755507571884605 56-57 0.026826537277223107 58-59 0.022982440692489357 60-61 0.013536476862481222 62-63 0.0020206148714626095 64-65 0.006423255404405533 66-67 0.1538131466788977 68-69 0.716119052656895 70-71 0.8778996644793648 72-73 0.35480682921828816 74-75 0.05283168639531507 76-77 0.19578608161049904 78-79 0.014341437258429741 80-81 0.0033841192156203056 82-83 0.0016920596078101528 84-85 1.149943422783599E-4 86-87 0.0017084873709927758 88-89 4.435496059308168E-4 90-91 0.004320501717029808 92-93 0.004369785006577676 94-95 0.07587983814053491 96-97 0.0373238779509191 98-99 0.013569332388846467 100-101 0.013355771467472372 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3043628.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.044405418746244 #Duplication Level Percentage of deduplicated Percentage of total 1 71.3098932168374 36.39971099727751 2 14.985768655436521 15.29879301519283 3 5.667487442751765 8.678805802004234 4 2.771206491566427 5.658183506183124 5 1.5274546064587355 3.8984006095405594 6 0.930925459959701 2.851112195568948 7 0.6069185416747714 2.1685857268180886 8 0.41676745276002547 1.7018917459216731 9 0.283815284083584 1.303846418231919 >10 1.3633835407625008 12.232980430354491 >50 0.08782267589522348 3.078553260740298 >100 0.04556584335358997 4.098686106223794 >500 0.0022755999153937097 0.7170515611726713 >1k 5.201371232013402E-4 0.6228319282453562 >5k 6.501714040016753E-5 0.3245748525731115 >10k+ 1.3003428080033506E-4 0.9659918439514884 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15671 0.5148789536697651 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13436 0.44144685224344105 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9780 0.3213270478521028 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4719 0.1550452289175944 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3504 0.11512576438382088 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 3.2855526365245684E-5 0.0 12-13 0.0 0.0 0.0 4.9283289547868526E-5 0.0 14-15 0.0 0.0 0.0 8.213881591311422E-5 0.0 16-17 0.0 0.0 0.0 1.4784986864360559E-4 0.0 18-19 1.6427763182622842E-5 0.0 0.0 1.6427763182622843E-4 0.0 20-21 3.2855526365245684E-5 1.6427763182622842E-5 0.0 1.6427763182622843E-4 0.0 22-23 3.2855526365245684E-5 3.2855526365245684E-5 0.0 5.092606586613081E-4 0.0 24-25 6.571105273049137E-5 3.2855526365245684E-5 0.0 0.0010678046068704847 0.0 26-27 6.571105273049137E-5 3.2855526365245684E-5 0.0 0.001692059607810153 0.0 28-29 1.4784986864360559E-4 3.2855526365245684E-5 0.0 0.003104847241515717 0.0 30-31 1.9713315819147413E-4 3.2855526365245684E-5 0.0 0.007408921195362903 0.0 32-33 1.9713315819147413E-4 3.2855526365245684E-5 0.0 0.015803508181683176 0.0 34-35 1.9713315819147413E-4 3.2855526365245684E-5 0.0 0.029109996359607676 0.0 36-37 2.299886845567198E-4 3.2855526365245684E-5 0.0 0.051928159420270806 0.0 38-39 2.628442109219655E-4 3.2855526365245684E-5 0.0 0.08576935157647386 0.0 40-41 2.628442109219655E-4 3.2855526365245684E-5 0.0 0.12143402544594806 0.0 42-43 2.792719741045883E-4 3.2855526365245684E-5 0.0 0.15880718668641503 0.0 44-45 3.2855526365245687E-4 3.2855526365245684E-5 0.0 0.2045913626763849 0.0 46-47 4.764051322960625E-4 3.2855526365245684E-5 0.0 0.2606593184186767 0.0 48-49 4.928328954786854E-4 3.2855526365245684E-5 0.0 0.32338051824993064 0.0 50-51 4.928328954786854E-4 3.2855526365245684E-5 0.0 0.39053721414049286 0.0 52-53 4.928328954786854E-4 3.2855526365245684E-5 0.0 0.4568560941087413 0.0 54-55 4.928328954786854E-4 3.2855526365245684E-5 0.0 0.5288096968486293 0.0 56-57 4.928328954786854E-4 3.2855526365245684E-5 0.0 0.6171089239552271 0.0 58-59 4.928328954786854E-4 3.2855526365245684E-5 0.0 0.705096023561355 0.0 60-61 4.928328954786854E-4 3.2855526365245684E-5 0.0 0.7929845565883873 0.0 62-63 5.25688421843931E-4 3.2855526365245684E-5 0.0 0.8815794834322723 0.0 64-65 5.25688421843931E-4 3.2855526365245684E-5 0.0 0.9786675638415734 0.0 66-67 5.25688421843931E-4 3.2855526365245684E-5 0.0 1.0823596050502888 0.0 68-69 5.25688421843931E-4 3.2855526365245684E-5 0.0 1.2028901035211925 0.0 70-71 5.25688421843931E-4 3.2855526365245684E-5 0.0 1.3534669808531135 0.0 72-73 5.25688421843931E-4 3.2855526365245684E-5 0.0 1.514606909911461 0.0 74-75 5.25688421843931E-4 3.2855526365245684E-5 0.0 1.6911232253087434 0.0 76-77 5.25688421843931E-4 3.2855526365245684E-5 0.0 1.8918376358740292 0.0 78-79 5.25688421843931E-4 3.2855526365245684E-5 0.0 2.1264260941218835 0.0 80-81 5.585439482091766E-4 3.2855526365245684E-5 0.0 2.382945616218539 0.0 82-83 5.913994745744223E-4 3.2855526365245684E-5 0.0 2.6550386578123213 0.0 84-85 6.406827641222909E-4 3.2855526365245684E-5 0.0 2.9242896963755096 0.0 86-87 6.735382904875366E-4 4.9283289547868526E-5 0.0 3.2119069741768707 0.0 88-89 6.899660536701594E-4 6.571105273049137E-5 0.0 3.521389604774302 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8450 0.0 43.81549 1 GTATCAA 13750 0.0 36.074753 1 ATCAACG 17695 0.0 27.888792 3 TATCAAC 17970 0.0 27.438751 2 TCAACGC 18010 0.0 27.32826 4 CAACGCA 18415 0.0 26.624073 5 AACGCAG 19085 0.0 25.714249 6 ACGCAGA 21950 0.0 22.206713 7 GTGGTAT 4885 0.0 22.057236 1 CGCAGAG 22080 0.0 21.968592 8 TGGTATC 4910 0.0 21.843582 2 GTACATG 13910 0.0 21.22526 1 TACATGG 13900 0.0 20.758009 2 GAGTACT 13050 0.0 20.433266 12-13 GCAGAGT 23265 0.0 20.115913 9 AGTACTT 13575 0.0 19.67795 12-13 ACATGGG 14040 0.0 19.43335 3 GTACTTT 14010 0.0 19.016209 14-15 AGAGTAC 21605 0.0 18.268618 10-11 GTATAGG 740 0.0 17.960403 1 >>END_MODULE