FastQCFastQC Report
Fri 27 May 2016
SRR939419_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939419_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2880065
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT221120.7677604498509581No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167480.5815146533151161No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119760.4158239484178309No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA63130.21919644174697447No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39700.13784411115721346No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34520.11985840597347629No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30010.10419903717450822No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA282700.033.441931
GGTATCA184400.031.4098551
TATCAAC348950.027.0279372
ATCAACG363050.025.8640753
TCAACGC366300.025.7733634
CAACGCA366950.025.6719255
AACGCAG371400.025.236046
GTACATG202050.025.2102181
TACATGG205650.024.297752
GAGTACT192600.023.40809412-13
AGTACTT195400.022.8396112-13
ACATGGG209550.022.8053323
ACGCAGA405100.022.6705027
CGCAGAG405200.022.6558918
GTACTTT207950.021.96827514-15
GCAGAGT394900.021.7811249
CATGGGA137250.020.7298164
ACTTTTT242100.019.9866316-17
AGAGTAC336000.019.97264310-11
TACTTTT226900.019.94567914-15
Produced by FastQC (version 0.11.5)