FastQCFastQC Report
Fri 27 May 2016
SRR939419_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939419_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2880065
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT306311.0635523851024196No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT265730.9226527873502856No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194680.6759569662490256No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA95610.3319716742504075No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66800.23193920970533655No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59950.20815502427896593No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47240.16402407584551043No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT42530.14767027827496948No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29040.10083105763237983No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA28840.10013662886080696No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA210050.033.398581
GTATCAA308350.029.4998651
GTACATG219300.023.6897741
TATCAAC386400.023.5104452
ATCAACG388550.023.3161323
TCAACGC392200.023.0628974
CAACGCA396650.022.7324815
TACATGG224200.022.722112
AACGCAG402000.022.4535226
GAGTACT230600.022.33615312-13
AGTACTT233500.021.7137612-13
GTACTTT238950.021.45647414-15
ACATGGG222450.021.3427723
ACGCAGA450750.019.9725447
CATGGGA148000.019.8813294
CGCAGAG451850.019.871498
TACTTTT256200.019.70663514-15
ACTTTTT267500.019.68900716-17
ATGGGAG46600.019.4206145
GCAGAGT451950.019.2275739
Produced by FastQC (version 0.11.5)