##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939419_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2880065 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.980360512696762 31.0 31.0 31.0 30.0 34.0 2 31.21508820113435 31.0 31.0 34.0 30.0 34.0 3 31.18223442873685 31.0 31.0 34.0 28.0 34.0 4 35.13856527543649 35.0 35.0 37.0 33.0 37.0 5 34.76801079142311 35.0 35.0 37.0 32.0 37.0 6 34.73911838795305 35.0 35.0 37.0 32.0 37.0 7 34.6044068449844 35.0 35.0 37.0 30.0 37.0 8 34.63364646284025 35.0 35.0 37.0 30.0 37.0 9 35.944781801799614 37.0 35.0 39.0 30.0 39.0 10-11 35.80284264417644 37.0 35.0 39.0 30.0 39.0 12-13 35.75725356892987 37.0 35.0 39.0 30.0 39.0 14-15 36.535962730007824 38.0 35.0 40.0 30.0 41.0 16-17 36.571941084663024 38.0 35.0 40.0 30.5 41.0 18-19 36.608145475883354 38.0 35.0 40.0 30.0 41.0 20-21 36.54343408916118 38.0 35.0 40.0 30.0 41.0 22-23 36.39914463736062 38.0 35.0 40.0 30.0 41.0 24-25 36.15665549215035 38.0 34.0 40.0 29.5 41.0 26-27 35.7579148387276 38.0 34.0 40.0 27.5 41.0 28-29 35.459007696006864 38.0 34.0 40.0 27.0 41.0 30-31 35.18272278577046 37.5 34.0 40.0 27.0 41.0 32-33 34.88600708664561 37.0 33.0 40.0 27.0 41.0 34-35 34.74293132273056 37.0 33.0 40.0 25.5 41.0 36-37 34.651284953638196 37.0 33.0 40.0 25.0 41.0 38-39 34.58975734922649 37.0 33.0 40.0 25.0 41.0 40-41 34.31725308282972 37.0 33.0 40.0 24.5 41.0 42-43 33.93357007567538 37.0 33.0 40.0 23.5 41.0 44-45 33.65140127045744 37.0 33.0 40.0 23.0 41.0 46-47 33.17161522396196 37.0 31.5 40.0 20.0 41.0 48-49 32.73761859541365 37.0 31.0 40.0 17.0 41.0 50-51 32.42198960787343 36.0 31.0 39.5 15.0 41.0 52-53 32.027695729089444 36.0 30.5 39.0 12.5 40.5 54-55 31.799736811495574 36.0 30.0 39.0 10.5 40.0 56-57 31.40081508577063 35.0 30.0 39.0 8.0 40.0 58-59 30.968371026348365 35.0 29.0 39.0 7.5 40.0 60-61 30.66798127820032 35.0 28.0 38.0 7.0 40.0 62-63 30.27978257435162 34.5 28.0 38.0 3.5 40.0 64-65 29.84705171584669 34.0 28.0 38.0 2.0 40.0 66-67 28.24743278363509 33.0 24.5 36.5 2.0 39.5 68-69 24.780976297409953 29.5 13.0 35.0 2.0 38.0 70-71 24.199048111761364 29.0 10.5 34.5 2.0 38.0 72-73 25.900556584660414 31.0 20.0 35.0 2.0 37.5 74-75 26.46108264917632 31.0 22.0 35.0 2.0 37.0 76-77 26.46865643657348 31.5 23.5 34.0 2.0 36.0 78-79 27.277885394947685 32.5 26.0 35.0 2.0 37.0 80-81 27.136423309890574 33.0 25.5 35.0 2.0 36.0 82-83 26.746690786492664 32.0 25.0 35.0 2.0 36.0 84-85 26.42909448224259 32.0 24.5 35.0 2.0 35.5 86-87 25.992621520694847 32.0 23.5 34.5 2.0 35.0 88-89 25.515361285248773 32.0 21.0 34.0 2.0 35.0 90-91 25.07964750795555 31.0 20.0 34.0 2.0 35.0 92-93 24.548057943136698 31.0 17.5 34.0 2.0 35.0 94-95 23.247373930796698 30.0 4.5 34.0 2.0 35.0 96-97 22.4654900149823 30.0 2.0 34.0 2.0 35.0 98-99 22.137413044497258 30.0 2.0 34.0 2.0 35.0 100-101 20.94463701340074 28.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3917717550033615 1101 2 0.25974452288274463 1101 3 0.27429343063758793 1101 4 0.16930412173913822 1101 5 0.34766145735520837 1101 6 0.5614617382917473 1101 7 0.5222792111448911 1101 8 0.4349383183348223 1101 9 0.6129906085249672 1101 10-11 0.7371810752893637 1101 12-13 0.6512061033983869 1101 14-15 0.8260521871562148 1101 16-17 0.7445667720438536 1101 18-19 0.8338986529515893 1101 20-21 0.7766417901377523 1101 22-23 0.8336098633295634 1101 24-25 0.7241552863853471 1101 26-27 0.8685149602821269 1101 28-29 0.7602568642813026 1101 30-31 0.8087000889056313 1101 32-33 0.7186496228890817 1101 34-35 0.7436146316799963 1101 36-37 0.9234180734024875 1101 38-39 0.7821439581809244 1101 40-41 0.8666019203412816 1101 42-43 0.9960716437914812 1101 44-45 0.7707473357570933 1101 46-47 0.8747021305645575 1101 48-49 0.8545187582544216 1101 50-51 0.760371855867092 1101 52-53 0.7993352287518967 1101 54-55 0.720299312407537 1101 56-57 0.5480160754392536 1101 58-59 0.6067043346870236 1101 60-61 0.35342560516380317 1101 62-63 0.2478948733319548 1101 64-65 0.29947256699830405 1101 66-67 -2.646947229613634 1101 68-69 -7.59170717265722 1101 70-71 -10.796499598980247 1101 72-73 -10.789125997185351 1101 74-75 -9.318207840154752 1101 76-77 -4.428158561083773 1101 78-79 -1.4964143244212167 1101 80-81 -0.7934881140219936 1101 82-83 -0.5318499104149872 1101 84-85 -0.5503071961075783 1101 86-87 -0.5342321828175294 1101 88-89 -0.5015751516340359 1101 90-91 -0.7107006482193903 1101 92-93 -1.3544885946063303 1101 94-95 -4.765632223058123 1101 96-97 -1.8343110094078092 1101 98-99 -1.105103722344925 1101 100-101 -0.7738537251900333 1102 1 0.4403061376789488 1102 2 0.25327860281282355 1102 3 0.4794762362723404 1102 4 0.2973072255387308 1102 5 0.4229075368008921 1102 6 0.4999264105236634 1102 7 0.5103174729118152 1102 8 0.573920553754995 1102 9 0.7454190618639842 1102 10-11 0.6650917758951209 1102 12-13 0.6685631285420612 1102 14-15 0.6326453590603833 1102 16-17 0.7976655368421888 1102 18-19 0.6375352846314115 1102 20-21 0.8925938128726187 1102 22-23 0.8048197528406149 1102 24-25 0.6470558779237408 1102 26-27 0.748232689585528 1102 28-29 0.7976915107403642 1102 30-31 0.5263754246240708 1102 32-33 0.566048282121379 1102 34-35 0.6615868883450062 1102 36-37 0.8791035352999899 1102 38-39 0.8091551097458591 1102 40-41 0.8553634806126453 1102 42-43 1.0473452151617693 1102 44-45 0.8968989204323776 1102 46-47 0.9191596501048096 1102 48-49 0.6377098670137755 1102 50-51 0.8111394802625895 1102 52-53 0.8211335633883543 1102 54-55 0.666514598686323 1102 56-57 0.6053824001351238 1102 58-59 0.6389256706108064 1102 60-61 0.5092490466167732 1102 62-63 0.6074897340295351 1102 64-65 0.4977567239310474 1102 66-67 -2.6505005404648223 1102 68-69 -7.3220826823885155 1102 70-71 -10.640813051147774 1102 72-73 -8.979876891600885 1102 74-75 -7.531280950562092 1102 76-77 -3.828330369158916 1102 78-79 -1.0544262868175913 1102 80-81 -0.4094778154096197 1102 82-83 -0.18198091366094715 1102 84-85 -0.260079064205307 1102 86-87 -0.14684956251567982 1102 88-89 -0.12015349164093436 1102 90-91 -0.11070512358775275 1102 92-93 -0.46287954830733113 1102 94-95 -4.249798139442447 1102 96-97 -1.000022875978651 1102 98-99 -0.17872583540325948 1102 100-101 -0.19472279565602335 1103 1 0.36705833360952766 1103 2 0.24646549023495368 1103 3 0.2948474976310358 1103 4 0.20269816351358827 1103 5 0.412424853233901 1103 6 0.31758204615586294 1103 7 0.48480188829057624 1103 8 0.3928253512778994 1103 9 0.6872605943489347 1103 10-11 0.6759463441382749 1103 12-13 0.6182889952060293 1103 14-15 0.7492274145929159 1103 16-17 0.6994325997366104 1103 18-19 0.6801171329530007 1103 20-21 0.6225093028169937 1103 22-23 0.6932728810519677 1103 24-25 0.9158181547508235 1103 26-27 0.6391354975464694 1103 28-29 0.8785743220032032 1103 30-31 0.8047212074602683 1103 32-33 0.9003315428466507 1103 34-35 0.9808448289041038 1103 36-37 1.0836024133285491 1103 38-39 0.9695763881194353 1103 40-41 1.0901490594050287 1103 42-43 1.2082798881222487 1103 44-45 1.0148257412774626 1103 46-47 1.1704947287270429 1103 48-49 1.3257217875391092 1103 50-51 1.082506423884368 1103 52-53 1.2388278615497335 1103 54-55 1.19559109490093 1103 56-57 1.375232534401075 1103 58-59 1.3308211116331812 1103 60-61 1.0736625891152336 1103 62-63 1.0163375569669455 1103 64-65 0.7178720208154701 1103 66-67 -2.6185134043895637 1103 68-69 -7.114521336157566 1103 70-71 -10.910337207226469 1103 72-73 -8.82485889120331 1103 74-75 -6.961637648192475 1103 76-77 -3.4128806456086345 1103 78-79 -0.5869209212334745 1103 80-81 -0.009684520957815579 1103 82-83 0.2504794804104584 1103 84-85 0.22391368004248235 1103 86-87 0.2777302878207415 1103 88-89 0.3014851847023152 1103 90-91 0.23848237367470304 1103 92-93 0.3302458075732524 1103 94-95 1.1174459803153667 1103 96-97 1.1460353570342647 1103 98-99 0.8938123133101783 1103 100-101 0.929541226459957 1104 1 0.41623899894171856 1104 2 0.14130978741699352 1104 3 0.33047791309929764 1104 4 0.22365457956976087 1104 5 0.45145863035806855 1104 6 0.5706214603486615 1104 7 0.6207603505219694 1104 8 0.49224826399390764 1104 9 0.647422962675293 1104 10-11 0.7423830185148077 1104 12-13 0.7288921763703513 1104 14-15 0.8019982528495504 1104 16-17 0.7474043954550353 1104 18-19 0.8236555010026976 1104 20-21 0.7722308665395019 1104 22-23 0.6368123401206418 1104 24-25 0.7326944274347511 1104 26-27 0.8892531423288048 1104 28-29 0.9868123592888765 1104 30-31 0.9604997791887868 1104 32-33 0.7699962541877241 1104 34-35 0.914537224131287 1104 36-37 1.166902535769708 1104 38-39 1.117446101514929 1104 40-41 1.1000547912482048 1104 42-43 1.1941591176734647 1104 44-45 1.0463782159966968 1104 46-47 0.8494656878603521 1104 48-49 0.7510091166505433 1104 50-51 0.7847630216672528 1104 52-53 0.8238760845886013 1104 54-55 0.7426435527402511 1104 56-57 0.7838891318545969 1104 58-59 0.7285534183931333 1104 60-61 0.4533019929330564 1104 62-63 0.62477969714271 1104 64-65 0.3819422338564813 1104 66-67 -2.5412816118495023 1104 68-69 -7.223578358161564 1104 70-71 -8.508580893108517 1104 72-73 -7.876560633787054 1104 74-75 -6.638831996137775 1104 76-77 -3.4502510304954264 1104 78-79 -0.7207410891927601 1104 80-81 -0.01950822052495127 1104 82-83 0.15206740707092692 1104 84-85 0.131341234323763 1104 86-87 0.28179227499035875 1104 88-89 0.34238295745911884 1104 90-91 0.4042874098183127 1104 92-93 0.4568771897254962 1104 94-95 1.1651562402578435 1104 96-97 1.4519222895911774 1104 98-99 1.1468553299241329 1104 100-101 1.1369824287711054 1105 1 0.48188811045950786 1105 2 0.35824556381171035 1105 3 0.463751474631124 1105 4 0.45553993247272473 1105 5 0.5085754025988152 1105 6 0.6168913925366937 1105 7 0.693884762623604 1105 8 0.6110376493416894 1105 9 0.7966867448572827 1105 10-11 0.9353862338961818 1105 12-13 0.8587123281429143 1105 14-15 0.9902102630879526 1105 16-17 1.006471709433228 1105 18-19 0.9794361173148616 1105 20-21 1.0475325775158595 1105 22-23 1.0783183231071973 1105 24-25 1.2185790261883511 1105 26-27 1.1803711892459106 1105 28-29 1.1948373896700275 1105 30-31 1.169425075366611 1105 32-33 1.2381277056998172 1105 34-35 1.2520174225873362 1105 36-37 1.0540843533926108 1105 38-39 0.9743381659373114 1105 40-41 1.1708846834335418 1105 42-43 1.3286632952100632 1105 44-45 1.0990635410289258 1105 46-47 1.1366992507857105 1105 48-49 0.8897221853621531 1105 50-51 0.8577308658042568 1105 52-53 0.980445278816898 1105 54-55 1.018903217840549 1105 56-57 1.0975452807335238 1105 58-59 1.1494809606244765 1105 60-61 1.2589691794436213 1105 62-63 1.3437682762884293 1105 64-65 1.2403948546279686 1105 66-67 1.8310588333035547 1105 68-69 -4.838571995026353 1105 70-71 -5.091732315233376 1105 72-73 -1.4400161543447965 1105 74-75 1.2588275792023573 1105 76-77 1.3541653542191128 1105 78-79 1.3625171024472493 1105 80-81 1.331078397012714 1105 82-83 1.5351625753917375 1105 84-85 1.3892535521649307 1105 86-87 1.4653203619306616 1105 88-89 1.3897071617598193 1105 90-91 1.3838052870518105 1105 92-93 1.517739911999751 1105 94-95 2.4311278121059416 1105 96-97 2.433986855432721 1105 98-99 2.1393129141460143 1105 100-101 1.8872446391254947 1106 1 0.1141802717007856 1106 2 0.16858417703215522 1106 3 0.1843079099976883 1106 4 0.15403203510531682 1106 5 0.3878475317176324 1106 6 0.2095123137958197 1106 7 0.2877645453834603 1106 8 0.17326772009074176 1106 9 0.4520770593122947 1106 10-11 0.42042447759600066 1106 12-13 0.3486644099255116 1106 14-15 0.4625564586796358 1106 16-17 0.28436209280960156 1106 18-19 0.31565312057306016 1106 20-21 0.4249723683216473 1106 22-23 0.47314319647056635 1106 24-25 0.41202506382248316 1106 26-27 0.6199230676339056 1106 28-29 0.7058866587461878 1106 30-31 0.551577811238495 1106 32-33 0.5112279010280716 1106 34-35 0.6632972974465545 1106 36-37 0.6237889206838645 1106 38-39 0.5874656439156638 1106 40-41 0.5805643573486989 1106 42-43 0.7141329866567716 1106 44-45 0.5647535153697589 1106 46-47 0.5295405672552604 1106 48-49 0.5538452452257232 1106 50-51 0.628810184595693 1106 52-53 0.6382916954674833 1106 54-55 0.6920315432964337 1106 56-57 0.8725138576962159 1106 58-59 0.7074879895826776 1106 60-61 0.7045835853363478 1106 62-63 0.784489316296515 1106 64-65 0.8091027679238678 1106 66-67 1.502214220861152 1106 68-69 -5.2936548180863205 1106 70-71 -5.86970274949924 1106 72-73 -2.054912971402988 1106 74-75 0.6106065672733472 1106 76-77 0.8878110800223524 1106 78-79 0.603992040086176 1106 80-81 0.6957015707241645 1106 82-83 0.768211186907017 1106 84-85 0.7952093791855752 1106 86-87 0.9305983907183553 1106 88-89 0.8257341973140555 1106 90-91 0.8056350318793939 1106 92-93 0.819280074776696 1106 94-95 1.8512886898884489 1106 96-97 1.841436591336258 1106 98-99 1.6795019322667208 1106 100-101 1.5054315579524626 1107 1 -0.04775637761622065 1107 2 -0.011204712733963618 1107 3 -0.020199253866849176 1107 4 -0.03493231929387974 1107 5 0.16227050933223808 1107 6 0.04873944238555339 1107 7 0.14670391106972147 1107 8 0.07594025182942232 1107 9 0.10634603526658282 1107 10-11 0.18375598103833823 1107 12-13 0.15270161846007113 1107 14-15 0.09794535250080116 1107 16-17 0.009890833515896702 1107 18-19 0.26835217907519393 1107 20-21 0.207924102309768 1107 22-23 0.346073292279236 1107 24-25 0.43361711199889896 1107 26-27 0.3464142675170834 1107 28-29 0.3464421530323136 1107 30-31 0.285831097439079 1107 32-33 0.2689750625210223 1107 34-35 0.34607463370893754 1107 36-37 0.42414212958256314 1107 38-39 0.3642077293298911 1107 40-41 0.3515925646013116 1107 42-43 0.3189587072032296 1107 44-45 -0.06296934349137473 1107 46-47 -0.18361336917615034 1107 48-49 -0.005865931005068603 1107 50-51 0.10392269422002443 1107 52-53 0.2167839328691592 1107 54-55 0.21562438835094966 1107 56-57 0.3820342572944391 1107 58-59 0.31914963812912234 1107 60-61 0.25975331412643143 1107 62-63 0.30648702273755646 1107 64-65 0.4617609380390135 1107 66-67 1.1336563562159476 1107 68-69 -5.602617935491491 1107 70-71 -5.584979799809158 1107 72-73 -2.2260172005401557 1107 74-75 0.13865714763141668 1107 76-77 0.23593314798554843 1107 78-79 0.2698146733699822 1107 80-81 0.17028159947608046 1107 82-83 0.34960728826638743 1107 84-85 0.3851273249071383 1107 86-87 0.46153778220945085 1107 88-89 0.32336859983826116 1107 90-91 0.265941603645679 1107 92-93 0.38770867969012457 1107 94-95 1.2597566103222917 1107 96-97 1.25365747791896 1107 98-99 0.8691312221553495 1107 100-101 0.6859507657151305 1108 1 0.06378145096902799 1108 2 0.07756583567059394 1108 3 0.12258921518354171 1108 4 0.053928122514953714 1108 5 -0.005607553815949018 1108 6 0.14960065366569353 1108 7 0.1410046083625076 1108 8 0.11923873912432015 1108 9 0.1918557663797884 1108 10-11 0.19704848626199833 1108 12-13 0.3192310776244227 1108 14-15 0.21560770191667444 1108 16-17 0.10509987017718458 1108 18-19 0.1182441953842499 1108 20-21 0.2512200531090585 1108 22-23 0.29478545258413646 1108 24-25 0.3257056097218367 1108 26-27 0.24353801339756131 1108 28-29 0.38497400705435325 1108 30-31 0.3226342282457466 1108 32-33 0.3078381828692116 1108 34-35 0.4234701153782936 1108 36-37 0.4299689165589342 1108 38-39 0.1863949738413737 1108 40-41 0.18296334364433875 1108 42-43 0.3675263075505484 1108 44-45 0.2423409216657575 1108 46-47 0.276150353214355 1108 48-49 0.2340383599135265 1108 50-51 0.21862343136623963 1108 52-53 0.4355623606436687 1108 54-55 0.23450839521856182 1108 56-57 0.1887273691260951 1108 58-59 0.5172416252583361 1108 60-61 0.4694918744805143 1108 62-63 0.5002116717322238 1108 64-65 0.49937595035927984 1108 66-67 1.3846478723483635 1108 68-69 -1.8636776650929399 1108 70-71 2.821993361429371 1108 72-73 1.9945436734266515 1108 74-75 1.3051984775952015 1108 76-77 0.6707361809849495 1108 78-79 0.38100911118008085 1108 80-81 0.3669031293725382 1108 82-83 0.4824852631445289 1108 84-85 0.2752250794186004 1108 86-87 0.37461781026918217 1108 88-89 0.5330888466940706 1108 90-91 0.49325823073963093 1108 92-93 0.4200398672834531 1108 94-95 1.355879292700891 1108 96-97 1.5723699263697597 1108 98-99 1.267387564053852 1108 100-101 1.224137504145009 1109 1 -0.020271650260291096 1109 2 -0.05917564967491984 1109 3 -0.028745125575618147 1109 4 -0.007551055577536658 1109 5 -0.01821782268871175 1109 6 0.1589324102523193 1109 7 0.052212981825149996 1109 8 -0.12216961469634668 1109 9 0.06475784386211814 1109 10-11 0.09947728107328402 1109 12-13 0.08741677753950938 1109 14-15 0.0049246906484015085 1109 16-17 0.055905009261358884 1109 18-19 0.08454396263089592 1109 20-21 0.248382474859433 1109 22-23 0.12598006049839228 1109 24-25 0.08004341450081398 1109 26-27 0.29063560854898896 1109 28-29 0.37486526083876015 1109 30-31 0.188402853869313 1109 32-33 0.03355781267861602 1109 34-35 0.2357191093333384 1109 36-37 0.25943777897741427 1109 38-39 0.17650679568301086 1109 40-41 0.1455015313205692 1109 42-43 0.002231049470765356 1109 44-45 -0.0940046526053564 1109 46-47 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2314 2 -0.25969172619725356 2314 3 -0.16637093192802155 2314 4 -0.14905471265273462 2314 5 -1.3100784001979449 2314 6 -2.6436269931924343 2314 7 -0.8106887115560895 2314 8 -0.2871463234275282 2314 9 0.020582143888368876 2314 10-11 0.250936843454447 2314 12-13 0.0045434389851806145 2314 14-15 -2.638092541924884 2314 16-17 -2.8182607667975645 2314 18-19 -0.37317120838226714 2314 20-21 -0.08097905000747119 2314 22-23 -0.009003029853715816 2314 24-25 -2.097953970242827 2314 26-27 -1.213385718806201 2314 28-29 -0.17006393172691503 2314 30-31 0.08950420521323821 2314 32-33 0.06605025853095015 2314 34-35 -0.014053524826245223 2314 36-37 0.19289949529294148 2314 38-39 -1.5949139222877875 2314 40-41 -4.2934070796938215 2314 42-43 -5.38495993344165 2314 44-45 -2.2144014554242517 2314 46-47 -3.193728347329248 2314 48-49 -0.98072111224635 2314 50-51 -0.9742424862988202 2314 52-53 -2.631405501758625 2314 54-55 -1.4020798014112579 2314 56-57 -3.487823946059553 2314 58-59 -4.715743286432215 2314 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82-83 -6.919879758403827 2315 84-85 -4.7083838799513344 2315 86-87 -5.392260777844168 2315 88-89 -3.9630266667913645 2315 90-91 -3.317594385517964 2315 92-93 -4.207691367360539 2315 94-95 -2.424342899382829 2315 96-97 -1.3194490238334922 2315 98-99 -2.857105665075604 2315 100-101 -2.3049016171130923 2316 1 -0.3291037878714782 2316 2 -0.375937160580019 2316 3 -0.9570320628290219 2316 4 -0.4320009708005017 2316 5 -0.4084877504430935 2316 6 -2.3468471102181283 2316 7 -0.4817879853993361 2316 8 -0.5508496881332903 2316 9 -0.10426914247373986 2316 10-11 -0.19857194563009983 2316 12-13 -0.847668421285654 2316 14-15 -1.226242605057628 2316 16-17 -1.7325176935965914 2316 18-19 -0.42979311188209124 2316 20-21 -4.185336728667913 2316 22-23 -4.251011137243637 2316 24-25 -2.8412271205056925 2316 26-27 -2.2982938246329923 2316 28-29 -1.4656111197582717 2316 30-31 -0.40863481186125483 2316 32-33 -1.9994975026449353 2316 34-35 -2.5660208000102998 2316 36-37 -5.001052614139454 2316 38-39 -2.4525999641342864 2316 40-41 -1.8936565712451454 2316 42-43 -3.155143522055873 2316 44-45 -0.9926461916127707 2316 46-47 -0.9772512236116597 2316 48-49 -0.8077397967580779 2316 50-51 -0.39890263764370815 2316 52-53 -2.6339086670795595 2316 54-55 -0.9534111901057223 2316 56-57 -0.20698269146354065 2316 58-59 -0.0924036687717198 2316 60-61 -1.5715341041019002 2316 62-63 -1.1569774125468726 2316 64-65 0.05858137677779496 2316 66-67 1.1090095914044156 2316 68-69 3.4569032566798974 2316 70-71 3.7988463109324684 2316 72-73 2.006281837485833 2316 74-75 0.8809208273816616 2316 76-77 -0.2116557900731273 2316 78-79 0.19863803750816444 2316 80-81 -0.4242266970769073 2316 82-83 -0.1398863473409726 2316 84-85 0.23318826167512086 2316 86-87 -0.2289354483490733 2316 88-89 0.3613147289056684 2316 90-91 0.5289878083376358 2316 92-93 0.7450387968515919 2316 94-95 1.0539975373971444 2316 96-97 0.8618055946930916 2316 98-99 -0.09572720733065765 2316 100-101 -0.2074649355449445 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 44.0 4 110.0 5 354.0 6 823.0 7 1796.0 8 3742.0 9 6241.0 10 10042.0 11 14725.0 12 18907.0 13 23603.0 14 28716.0 15 36923.0 16 44523.0 17 50059.0 18 43778.0 19 33379.0 20 30991.0 21 31292.0 22 32720.0 23 37475.0 24 44850.0 25 54935.0 26 68129.0 27 84691.0 28 103255.0 29 122204.0 30 143228.0 31 165672.0 32 192317.0 33 224298.0 34 260494.0 35 299464.0 36 306802.0 37 251871.0 38 101564.0 39 6039.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.68659462128945 19.849836197887814 15.486097918690389 20.97747126213235 2 16.22583114200502 21.977772776965146 38.102754197557104 23.69364188347273 3 19.647577398426773 26.210450111369017 30.658301114731785 23.48367137547243 4 13.017206208887647 17.85004852321041 35.9545704697637 33.17817479813824 5 13.093151087319965 38.84737938024273 32.655362052843984 15.404107479593328 6 30.73677156591952 36.5154605885631 16.82552303507039 15.922244810446987 7 27.44865133252201 35.183025383107676 19.475497948831016 17.892825335539303 8 27.56833613130259 33.398586490235466 18.951447276363552 20.08163010209839 9 27.33066788423178 16.495773532889014 20.062081932178614 36.11147665070059 10-11 25.319202865213114 27.49267811663973 25.370885726537423 21.81723329160974 12-13 27.039320293118386 25.18713987357924 26.84602604455108 20.927513788751295 14-15 23.605387378409862 25.608293562818897 27.03154269087677 23.754776367894475 16-17 21.263080922469136 29.26725654007062 27.353298706655742 22.116363830804502 18-19 20.93055676358758 26.46327979046041 31.846282076722503 20.759881369229504 20-21 22.361925859312205 25.173060330235604 31.944174871053256 20.520838939398935 22-23 21.85862124639548 25.50919857711545 31.82488936881633 20.807290807672743 24-25 21.5945647060049 25.68495849920054 32.07533510528408 20.645141689510478 26-27 21.346824307503102 25.82641823107871 32.09204079513925 20.734716666278942 28-29 20.885278198515003 26.086238561740256 32.32799178895346 20.700491450791283 30-31 20.929410917058238 25.973417872058207 32.06968541906653 21.027485791817025 32-33 20.82796338335062 26.16347276539041 31.795995433770134 21.212568417488832 34-35 21.486360680033574 26.04456074885782 31.64335427576716 20.82572429534145 36-37 21.421229148140807 25.81794307360545 31.710848658135255 21.049979120118493 38-39 21.309120168503885 26.01296103521446 31.841308129582547 20.83661066669911 40-41 21.341969385630303 26.0819718120866 31.731617247028893 20.844441555254196 42-43 21.183937703624032 26.312721676154567 31.137048238573378 21.366292381648027 44-45 21.271314402412997 26.548279338208474 30.843039213999617 21.33736704537891 46-47 21.48323769054556 26.783097032912913 30.158445766177454 21.57521951036407 48-49 21.75654210967179 26.947878615147737 29.67783867951744 21.61774059566304 50-51 21.5318519774891 27.327588364178308 29.530193411335524 21.610366246997064 52-53 21.94064969783819 27.455035372816226 29.037851406547016 21.566463522798564 54-55 21.36941300323904 27.238598865423917 29.32068755553664 22.0713005758004 56-57 21.69850331374489 27.694366103987385 28.81409770463185 21.79303287763588 58-59 21.874252293429837 27.179014452853288 29.00429533007436 21.942437923642512 60-61 21.73988757948651 27.145812444386568 29.509195477136096 21.60510449899082 62-63 21.83883860266627 27.182841330431263 29.2930397722094 21.68528029469306 64-65 21.520732726778714 27.286520233582266 29.25622259588132 21.936524443757698 66-67 21.184523512773946 27.494956206942113 29.421394049666418 21.899126230617526 68-69 21.21281582651862 27.11388433699944 29.880952901723806 21.79234693475813 70-71 20.82526453233527 27.232584261398678 30.024223039697272 21.917928166568775 72-73 21.62010714162616 27.324439564066505 28.90870749402999 22.146745800277344 74-75 21.151718406631616 27.761081671256505 28.879958413307744 22.20724150880414 76-77 21.362945234628 27.664679579348437 28.72936551357957 22.243009672443996 78-79 21.102324089859227 27.690228766391456 28.82557927756136 22.381867866187953 80-81 21.056664818875042 27.991577820181114 28.631991262543995 22.319766098399853 82-83 21.177247735728187 27.93244249695753 28.313041545937327 22.577268221376947 84-85 20.98313753335428 27.715971688138985 28.238807110256193 23.062083668250544 86-87 21.202765851398247 27.6362921730447 28.348015062914627 22.81292691264242 88-89 21.086538201125954 27.59740643031396 28.144481025650286 23.1715743429098 90-91 20.834531264974146 27.73578072503684 28.441240932178317 22.988447077810694 92-93 20.933420532119772 27.748727912182236 28.37580917701834 22.942042378679652 94-95 20.477786662602384 27.430045925032033 28.722979288151564 23.369188124214023 96-97 20.362978718073283 27.313760521601388 28.76089395506422 23.562366805261114 98-99 20.5086044163179 27.34442390206703 28.12940586536578 24.017565816249284 100-101 20.348515103148728 27.14983246502895 28.625379859655713 23.876272572166606 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 8616.0 1 5764.0 2 2997.5 3 3227.0 4 3084.0 5 3053.5 6 4947.0 7 9552.0 8 13988.0 9 15712.5 10 14206.5 11 11374.0 12 10119.5 13 9776.0 14 9563.5 15 9374.0 16 9259.0 17 9324.5 18 9576.5 19 9956.5 20 10488.5 21 11443.0 22 12557.5 23 13843.5 24 15273.0 25 17205.5 26 20191.0 27 23577.0 28 26684.5 29 30043.0 30 33963.5 31 37980.0 32 41854.5 33 45611.5 34 49669.5 35 53418.0 36 57849.5 37 63172.5 38 65726.0 39 66052.5 40 66884.0 41 71117.0 42 76343.5 43 81048.0 44 85904.5 45 89122.5 46 92508.0 47 100302.5 48 115694.5 49 118288.0 50 108227.5 51 102423.0 52 98326.5 53 97367.5 54 95193.5 55 89446.5 56 82849.0 57 75949.0 58 69727.0 59 62903.5 60 55057.5 61 45577.5 62 35407.5 63 26611.5 64 19558.5 65 14319.0 66 10395.5 67 7740.0 68 5484.5 69 3638.0 70 2539.5 71 1954.5 72 1614.0 73 1391.5 74 1094.5 75 809.5 76 561.0 77 358.0 78 232.5 79 131.0 80 76.0 81 45.5 82 32.0 83 19.5 84 10.5 85 7.0 86 3.0 87 2.0 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023471692479162795 2 0.01850652676241682 3 0.0 4 0.0 5 6.944287715728638E-5 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 4.166572629437183E-4 18-19 0.0012673325081204766 20-21 0.0 22-23 0.0 24-25 0.0 26-27 5.555430172582911E-4 28-29 0.01838500172739157 30-31 0.0042533762258837905 32-33 0.007899127276641327 34-35 0.00923590266191909 36-37 0.10046648252730406 38-39 0.10346988696435672 40-41 0.11951119158768986 42-43 0.1034525262450674 44-45 0.09772348887959126 46-47 0.0995116429663914 48-49 0.10190742222831775 50-51 0.10003246454507103 52-53 0.07706423292529857 54-55 0.06588392970297546 56-57 0.03720402143701618 58-59 0.014791332834502 60-61 0.008767163241107405 62-63 0.0017881540868001243 64-65 0.004774197804563438 66-67 0.40582417410718163 68-69 0.7840968867022099 70-71 0.8656749066427321 72-73 0.27054944940478776 74-75 0.143920362908476 76-77 0.013524000326381524 78-79 0.008662998925371477 80-81 0.001753432648221481 82-83 0.0 84-85 0.0 86-87 3.992965436543967E-4 88-89 6.597073329942207E-4 90-91 0.003506865296442962 92-93 0.001927039841114697 94-95 0.21704371255509858 96-97 0.22405744314798448 98-99 0.013228868098463055 100-101 0.007586634329433538 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2880065.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.75557698522722 #Duplication Level Percentage of deduplicated Percentage of total 1 67.62416109358229 26.88437542417319 2 14.525730065200845 11.549575597474439 3 6.3936795899321845 7.625532634692751 4 3.456474561315896 5.496565620794945 5 2.04820339691228 4.0713753913675035 6 1.3477378541519054 3.2148057609984604 7 0.9243125753521809 2.572260582347909 8 0.6795295037372073 2.161206999964624 9 0.4407994045436548 1.577181119613983 >10 2.3053695837535306 16.00530910181343 >50 0.1488037131757145 4.057640082242172 >100 0.09405865899050056 7.095192514744903 >500 0.00716142815489057 1.9372709798309597 >1k 0.003448095037528487 2.275193238336229 >5k 2.6523807980988363E-4 0.7815726378440352 >10k+ 2.6523807980988363E-4 2.6949423137604724 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 30631 1.0635523851024196 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26573 0.9226527873502856 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19468 0.6759569662490256 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 9561 0.3319716742504075 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6680 0.23193920970533655 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5995 0.20815502427896593 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4724 0.16402407584551043 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4253 0.14767027827496948 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2904 0.10083105763237983 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2884 0.10013662886080696 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 3.472143857864319E-5 0.0 12-13 0.0 0.0 0.0 3.472143857864319E-5 0.0 14-15 0.0 0.0 0.0 6.944287715728638E-5 0.0 16-17 0.0 0.0 0.0 1.0416431573592958E-4 0.0 18-19 0.0 0.0 0.0 1.0416431573592958E-4 0.0 20-21 0.0 0.0 0.0 2.951322279184671E-4 1.7360719289321596E-5 22-23 0.0 0.0 0.0 8.853966837554014E-4 3.472143857864319E-5 24-25 0.0 0.0 0.0 0.0017707933675108027 3.472143857864319E-5 26-27 0.0 0.0 0.0 0.0027950758055807767 3.472143857864319E-5 28-29 0.0 0.0 0.0 0.005815840961922734 3.472143857864319E-5 30-31 0.0 0.0 0.0 0.012933735870544588 3.472143857864319E-5 32-33 0.0 0.0 0.0 0.024235564127892947 6.944287715728638E-5 34-35 0.0 0.0 0.0 0.042221269311630116 6.944287715728638E-5 36-37 0.0 0.0 0.0 0.06831443040348048 6.944287715728638E-5 38-39 0.0 0.0 0.0 0.10796631326029099 6.944287715728638E-5 40-41 0.0 0.0 0.0 0.15188893306227463 1.0416431573592958E-4 42-43 0.0 0.0 0.0 0.19829413572263127 1.0416431573592958E-4 44-45 0.0 0.0 0.0 0.2537095516941458 1.0416431573592958E-4 46-47 0.0 0.0 0.0 0.3190552990991523 1.0416431573592958E-4 48-49 0.0 0.0 0.0 0.39146685925491265 1.0416431573592958E-4 50-51 0.0 0.0 0.0 0.4729580755989882 1.0416431573592958E-4 52-53 0.0 0.0 0.0 0.5499007834892615 1.0416431573592958E-4 54-55 0.0 0.0 0.0 0.6351071937612519 1.0416431573592958E-4 56-57 0.0 0.0 0.0 0.7312682179048042 1.0416431573592958E-4 58-59 0.0 0.0 0.0 0.8284361637671372 1.0416431573592958E-4 60-61 0.0 0.0 0.0 0.9352219481157544 1.2152503502525117E-4 62-63 0.0 0.0 0.0 1.035723152081637 1.3888575431457277E-4 64-65 0.0 0.0 0.0 1.1401478786069066 1.3888575431457277E-4 66-67 0.0 0.0 0.0 1.252193960900188 1.3888575431457277E-4 68-69 0.0 0.0 0.0 1.3883714430056266 1.3888575431457277E-4 70-71 0.0 0.0 0.0 1.5695131880704083 1.3888575431457277E-4 72-73 0.0 0.0 0.0 1.766539991284919 1.3888575431457277E-4 74-75 0.0 0.0 0.0 1.9763963660542383 1.3888575431457277E-4 76-77 0.0 0.0 0.0 2.208405018636732 1.3888575431457277E-4 78-79 0.0 0.0 0.0 2.4731039056410182 1.3888575431457277E-4 80-81 0.0 0.0 0.0 2.768756955138165 1.3888575431457277E-4 82-83 0.0 0.0 0.0 3.074288253910936 1.3888575431457277E-4 84-85 0.0 0.0 0.0 3.3778577914040135 1.5624647360389435E-4 86-87 0.0 0.0 0.0 3.6894479812087573 1.7360719289321596E-4 88-89 0.0 0.0 0.0 4.023867516878959 1.7360719289321596E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 21005 0.0 33.39858 1 GTATCAA 30835 0.0 29.499865 1 GTACATG 21930 0.0 23.689774 1 TATCAAC 38640 0.0 23.510445 2 ATCAACG 38855 0.0 23.316132 3 TCAACGC 39220 0.0 23.062897 4 CAACGCA 39665 0.0 22.732481 5 TACATGG 22420 0.0 22.72211 2 AACGCAG 40200 0.0 22.453522 6 GAGTACT 23060 0.0 22.336153 12-13 AGTACTT 23350 0.0 21.71376 12-13 GTACTTT 23895 0.0 21.456474 14-15 ACATGGG 22245 0.0 21.342772 3 ACGCAGA 45075 0.0 19.972544 7 CATGGGA 14800 0.0 19.881329 4 CGCAGAG 45185 0.0 19.87149 8 TACTTTT 25620 0.0 19.706635 14-15 ACTTTTT 26750 0.0 19.689007 16-17 ATGGGAG 4660 0.0 19.420614 5 GCAGAGT 45195 0.0 19.227573 9 >>END_MODULE