FastQCFastQC Report
Fri 27 May 2016
SRR939418_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939418_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2867806
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT217490.7583846327122546No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155330.5416335693558072No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116600.4065825931042755No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA63420.22114466599205107No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38090.1328193050715425No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34740.12113790123878673No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30350.10583003173854856No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA272350.034.604351
GGTATCA178000.031.4219781
TATCAAC336600.027.4619582
ATCAACG352450.026.348533
GTACATG194900.026.0555291
TCAACGC356600.025.96934
CAACGCA357450.025.3960635
AACGCAG363950.024.9183376
TACATGG198000.024.8394662
AACGCGG4750.024.5302246
CAACGCG5100.023.8218065
GAGTACT189200.023.31719812-13
ACATGGG204200.023.0571063
AGTACTT194050.022.59806312-13
ACGCAGA396350.022.4897947
CGCAGAG400050.022.2085748
GTACTTT206400.021.6640114-15
GCAGAGT385450.021.525739
AGAGTAC325500.020.18717610-11
CATGGGA136750.020.0857244
Produced by FastQC (version 0.11.5)